LOCUS       sequence-243639-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBC with 4 repeats of 80bp DNA template.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Chavan D, Chen H, Crum M, Vu B, Safari M, Smith M, Vekilov P, Conrad
            JC, Kourentzi K, Willson RC
  TITLE     Neutral DNA-avidin nanoparticles as ultrasensitive reporters in 
            immuno-PCR.
  JOURNAL   Analyst. 2020 Jul 21;145(14):4942-4949. doi: 10.1039/d0an00134a. 
            Epub 2020 Jun 5.
  PUBMED    32500871
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/d0an00134a"; journalName: "Analyst"; date: "2020-07-21- 
            21"; volume: "145"; issue: "14"; pages: "4942-4949"
COMMENT     Sequence Label: pMCRW2
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     100..117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    234..255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(270..275,276..293,294..300)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    308..324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     313..335
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     332..348
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     332..348
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     367..387
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        369..387
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     755..771
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     755..771
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(805..821)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(806..822)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
       61 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      121 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      181 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
      241 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
      301 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
      361 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agcttcaggt agtagtacgt
      421 ctgtttcacg atgagactgg atgaatcacg gtagcataag gtgcaagata atactctcgc
      481 agcagagctt caggtagtag tacgtctgtt tcacgatgag actggatgaa tcacggtagc
      541 ataaggtgca agataatact ctcgcagcag agcttcaggt agtagtacgt ctgtttcacg
      601 atgagactgg atgaatcacg gtagcataag gtgcaagata atactctcgc agcagagctt
      661 caggtagtag tacgtctgtt tcacgatgag actggatgaa tcacggtagc ataaggtgca
      721 agataatact ctcgcagcag agctcatgtc accatctaga actagtggat cccccgggct
      781 gcaggaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca
//
