LOCUS       sequence-243667-       12866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expressing nuclear TurboID-YFP under the UBQ10 
            promoter in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12866)
  AUTHORS   Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC
  TITLE     Proximity labeling of protein complexes and cell type-specific 
            organellar proteomes in Arabidopsis enabled by TurboID.
  JOURNAL   Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864.
  PUBMED    31535972
REFERENCE   2  (bases 1 to 12866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-19"; volume: "8. pii: 47864. doi"; pages: " 
            10.7554/eLife.47864"
COMMENT     Sequence Label: R4pGWB601_UBQ10p-Turbo-YFP-NLS
FEATURES             Location/Qualifiers
     source          1..12866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    322..342
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2361..2385
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2399..2440
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2447..3403
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             join(3431..3433,3434..3436,3437..4147)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3737..3756)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4084..4105
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4157..4177
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(4197..4221)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4253..4505
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4538..4554)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4538..4554)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4551..4573)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4562..4578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4586..4592,4593..4610,4611..4616))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4631..4652
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4688..4940)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5100..5651)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5703..5882)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6388..6412
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6933..7724
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7970..8558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8459..8478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8712..8729
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8744..8884
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8810..8829)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8970..8992
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9228..9422
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9488..10561)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10990..11619)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgtgg atcccccatc acaactttgt atagaaaagt tggctccgaa ttcgccctta
      361 gtctagctca acagagcttt taacccaaat tggtacaata gaatacaact ttagatcata
      421 attctcaaaa gaaagagatt ccttagctat tctatctgcc actccatttc cttctcggct
      481 tgtatgcaca agcataaaat cctcaaactt gctaagtaga tactttatgt cttggataat
      541 tggattgaga cttgacaagc ataactttca tgtaaccaaa gacacaagtt gctgagaatc
      601 cacctcaaaa atgatcttcc tataattgaa tcgggataat gacagcacag cccatctaag
      661 agcctccact tctacttcca gcacgcttct tacttttacc acagctcttg cacctaacca
      721 taacaccttc cctgtatgat cgcgaagcac ccaccctaag ccacatttta atccttctgt
      781 tggccatgcc ccatcaaagt tgcacttaac ccaagattgt ggtggagctt cccatgtttc
      841 tcgtctgtcc cgacggtgtt gtggttggtg ctttccttac attctgagcc tctttccttc
      901 taatccactc atctgcatct tcttgtgtcc ttactaatac ctcattggtt ccaaattccc
      961 tccctttaag caccagctcg tttctgttct tccacagcct cccaagtatc caagggacta
     1021 aagcctccac attcttcaga tcaggatatt cttgtttaag atgttgaact ctatggaggt
     1081 ttgtatgaac tgatgatcta ggaccggata agttcccttc ttcatagcga acttattcaa
     1141 agaatgtttt gtgtatcatt cttgttacat tgttattaat gaaaaaatat tattggtcat
     1201 tggactgaac acgagtgtta aatatggacc aggccccaaa taagatccat tgatatatga
     1261 attaaataac aagaataaat cgagtcacca aaccacttgc cttttttaac gagacttgtt
     1321 caccaacttg atacaaaagt cattatccta tgcaaatcaa taatcataca aaaatatcca
     1381 ataacactaa aaaattaaaa gaaatggata atttcacaat atgttatacg ataaagaagt
     1441 tacttttcca agaaattcac tgattttata agcccacttg cattagataa atggcaaaaa
     1501 aaaacaaaaa ggaaaagaaa taaagcacga agaattctag aaaatacgaa atacgcttca
     1561 atgcagtggg acccacggtt caattattgc caattttcag ctccaccgta tatttaaaaa
     1621 ataaaacgat aatgctaaaa aaatataaat cgtaacgatc gttaaatctc aacggctgga
     1681 tcttatgacg accgttagaa attgtggttg tcgacgagtc agtaataaac ggcgtcaaag
     1741 tggttgcagc cggcacacac gagtcgtgtt tatcaactca aagcacaaat acttttcctc
     1801 aacctaaaaa taaggcaatt agccaaaaac aactttgcgt gtaaacaacg ctcaatacac
     1861 gtgtcatttt attattagct attgcttcac cgccttagct ttctcgtgac ctagtcgtcc
     1921 tcgtcttttc ttcttcttct tctataaaac aatacccaaa gagctcttct tcttcacaat
     1981 tcagatttca atttctcaaa atcttaaaaa ctttctctca attctctcta ccgtgatcaa
     2041 ggtaaatttc tgtgttcctt attctctcaa aatcttcgat tttgttttcg ttcgatccca
     2101 atttcgtata tgttctttgg tttagattct gttaatctta gatcgaagac gattttctgg
     2161 gtttgatcgt tagatatcat cttaattctc gattagggtt tcatagatat catccgattt
     2221 gttcaaataa tttgagtttt gtcgaataat tactcttcga tttgtgattt ctatctagat
     2281 ctggtgttag tttctagttt gtgcgatcga atttgtcgat taatctgagt ttttctgatt
     2341 aacagaaggg cgaattcgac ccaagtttgt acaaaaaagc aggctccgcg gccgcatggg
     2401 caagcccatc cccaaccccc tgctgggcct ggacagcacc gctagcaaag acaatactgt
     2461 gcctctgaag ctgatcgctc tcctggctaa tggcgagttc catagtggcg aacagctggg
     2521 agaaaccctg ggcatgtcca gggccgctat caacaagcac attcagactc tgcgcgactg
     2581 gggcgtggac gtgttcaccg tgcccggaaa gggctactct ctgcccgagc ctatcccgct
     2641 gctgaacgct aaacagattc tgggacagct ggacggcggg agcgtggcag tcctgcctgt
     2701 ggtcgactcc accaatcagt acctgctgga tcgaatcggc gagctgaaga gtggggatgc
     2761 ttgcattgca gaatatcagc aggcagggag aggaagcaga gggaggaaat ggttctctcc
     2821 ttttggagct aacctgtacc tgagtatgtt ttggcgcctg aagcggggac cagcagcaat
     2881 cggcctgggc ccggtcatcg gaattgtcat ggcagaagcg ctgcgaaagc tgggagcaga
     2941 caaggtgcga gtcaaatggc ccaatgacct gtatctgcag gatagaaagc tggcaggcat
     3001 cctggtggag ctggccggaa taacaggcga tgctgcacag atcgtcattg gcgccgggat
     3061 taacgtggct atgaggcgcg tggaggaaag cgtggtcaat cagggctgga tcacactgca
     3121 ggaagcaggg attaacctgg acaggaatac tctggccgct acgctgatcc gagagctgcg
     3181 ggcagccctg gaactgttcg agcaggaagg cctggctcca tatctgccac ggtgggagaa
     3241 gctggataac ttcatcaata gacccgtgaa gctgatcatt ggggacaaag agattttcgg
     3301 gattagccgg gggattgata aacagggagc cctgctgctg gaacaggacg gagttatcaa
     3361 accctggatg ggcggagaaa tcagtctgcg gtctgccgaa aagggaggcg gtggaagcgg
     3421 cggaggttcc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     3481 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     3541 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     3601 ctggcccacc ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga
     3661 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     3721 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3781 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     3841 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     3901 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     3961 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     4021 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga
     4081 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     4141 gtacaaggct tcggatccaa aaaagaagag aaaggtatag aagggtgggc gcgccgaccc
     4201 agctttcttg tacaaagtgg ttgataacag cgcttagagc tcgaatttcc ccgatcgttc
     4261 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     4321 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     4381 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     4441 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     4501 agatcgggaa ttggttccgg aaccaattcg taatcatgtc atagctgttt cctgtgtgaa
     4561 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     4621 ggggtgccta atgagtgagc taactcacat taggctgaat taggcgcgcc tatttctatt
     4681 aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
     4741 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
     4801 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
     4861 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
     4921 tgccaaatgt ttgaacgatc ggggaaattc gagctgggga cgtccccagc tcggtacccc
     4981 ctcctgggca cggtcgtacg cgatccgccg cggttccacc tcaccattgc gggtatgcca
     5041 gttgagttcg atgagttccc gtaccagctc caactcgggc gatgccacgc gcactccgtt
     5101 cagatctcgg tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc
     5161 cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg
     5221 ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca
     5281 gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg
     5341 gagcccagtc ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag
     5401 tcgtaggcgt tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc
     5461 acctcggcga cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc
     5521 tgcggttcct gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg
     5581 atggtgcaga ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt
     5641 tctgggctca tatcttattg ccccccggga tcagatctgg attgagagtg aatatgagac
     5701 tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata
     5761 tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta
     5821 tgtgcttagc tcattaaact ccagaaaccc gcggctgagt ggctccttca acgttgcggt
     5881 tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga
     5941 ctcccttaat tctccgctca tgatcttgat cccctgcgcc atcagatcct tggcggcaag
     6001 aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc
     6061 aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca
     6121 ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat agcccagtag
     6181 ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt gttccgcttc
     6241 ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg aagctgatcc gtcgagatta
     6301 atagaaataa attcagccta attcggcgtt aattcagtac attaaaaacg tccgcaatgt
     6361 gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag
     6421 ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc
     6481 agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc
     6541 gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg
     6601 agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca attcgggcac
     6661 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     6721 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     6781 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     6841 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     6901 agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat
     6961 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     7021 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     7081 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     7141 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     7201 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     7261 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     7321 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     7381 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     7441 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     7501 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     7561 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     7621 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     7681 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt
     7741 cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac
     7801 ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat
     7861 aataagccct acacaaattg ggagatatat catgcatgac caaaatccct taacgtgagt
     7921 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7981 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8041 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8101 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8161 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8221 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8281 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8341 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8401 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8461 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8521 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8581 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8641 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8701 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     8761 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     8821 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     8881 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     8941 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     9001 gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg
     9061 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
     9121 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
     9181 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
     9241 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
     9301 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
     9361 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
     9421 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
     9481 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
     9541 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
     9601 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
     9661 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
     9721 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
     9781 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
     9841 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
     9901 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
     9961 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
    10021 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
    10081 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
    10141 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
    10201 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
    10261 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
    10321 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
    10381 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
    10441 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
    10501 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca
    10561 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc
    10621 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
    10681 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
    10741 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
    10801 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
    10861 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
    10921 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
    10981 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
    11041 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
    11101 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
    11161 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
    11221 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
    11281 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
    11341 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
    11401 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
    11461 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
    11521 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
    11581 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
    11641 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
    11701 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
    11761 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
    11821 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
    11881 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
    11941 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
    12001 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
    12061 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
    12121 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
    12181 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
    12241 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
    12301 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt
    12361 ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc
    12421 cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga
    12481 tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca
    12541 agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt
    12601 tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg
    12661 cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt
    12721 gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
    12781 agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac
    12841 atacaaatgg acgaacggat aaacct
//
