LOCUS       sequence-243668-       12692 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10
            promoter in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12692)
  AUTHORS   Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC
  TITLE     Proximity labeling of protein complexes and cell type-specific 
            organellar proteomes in Arabidopsis enabled by TurboID.
  JOURNAL   Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864.
  PUBMED    31535972
REFERENCE   2  (bases 1 to 12692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-19"; volume: "8. pii: 47864. doi"; pages: " 
            10.7554/eLife.47864"
COMMENT     Sequence Label: R4pGWB601_UBQ10p-miniTurbo-NES-YFP
FEATURES             Location/Qualifiers
     source          1..12692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    322..342
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2361..2385
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2399..2440
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2447..3217
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             3224..3250
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(3281..3283,3284..3286,3287..4000)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3587..3606)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3934..3955
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4023..4047)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4079..4331
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4364..4380)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4364..4380)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4377..4399)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4388..4404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4412..4418,4419..4436,4437..4442))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4457..4478
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4514..4766)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4926..5477)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5529..5708)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6214..6238
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6759..7550
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7796..8384
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8285..8304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8538..8555
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8570..8710
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8636..8655)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8796..8818
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9054..9248
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9314..10387)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10816..11445)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgtgg atcccccatc acaactttgt atagaaaagt tggctccgaa ttcgccctta
      361 gtctagctca acagagcttt taacccaaat tggtacaata gaatacaact ttagatcata
      421 attctcaaaa gaaagagatt ccttagctat tctatctgcc actccatttc cttctcggct
      481 tgtatgcaca agcataaaat cctcaaactt gctaagtaga tactttatgt cttggataat
      541 tggattgaga cttgacaagc ataactttca tgtaaccaaa gacacaagtt gctgagaatc
      601 cacctcaaaa atgatcttcc tataattgaa tcgggataat gacagcacag cccatctaag
      661 agcctccact tctacttcca gcacgcttct tacttttacc acagctcttg cacctaacca
      721 taacaccttc cctgtatgat cgcgaagcac ccaccctaag ccacatttta atccttctgt
      781 tggccatgcc ccatcaaagt tgcacttaac ccaagattgt ggtggagctt cccatgtttc
      841 tcgtctgtcc cgacggtgtt gtggttggtg ctttccttac attctgagcc tctttccttc
      901 taatccactc atctgcatct tcttgtgtcc ttactaatac ctcattggtt ccaaattccc
      961 tccctttaag caccagctcg tttctgttct tccacagcct cccaagtatc caagggacta
     1021 aagcctccac attcttcaga tcaggatatt cttgtttaag atgttgaact ctatggaggt
     1081 ttgtatgaac tgatgatcta ggaccggata agttcccttc ttcatagcga acttattcaa
     1141 agaatgtttt gtgtatcatt cttgttacat tgttattaat gaaaaaatat tattggtcat
     1201 tggactgaac acgagtgtta aatatggacc aggccccaaa taagatccat tgatatatga
     1261 attaaataac aagaataaat cgagtcacca aaccacttgc cttttttaac gagacttgtt
     1321 caccaacttg atacaaaagt cattatccta tgcaaatcaa taatcataca aaaatatcca
     1381 ataacactaa aaaattaaaa gaaatggata atttcacaat atgttatacg ataaagaagt
     1441 tacttttcca agaaattcac tgattttata agcccacttg cattagataa atggcaaaaa
     1501 aaaacaaaaa ggaaaagaaa taaagcacga agaattctag aaaatacgaa atacgcttca
     1561 atgcagtggg acccacggtt caattattgc caattttcag ctccaccgta tatttaaaaa
     1621 ataaaacgat aatgctaaaa aaatataaat cgtaacgatc gttaaatctc aacggctgga
     1681 tcttatgacg accgttagaa attgtggttg tcgacgagtc agtaataaac ggcgtcaaag
     1741 tggttgcagc cggcacacac gagtcgtgtt tatcaactca aagcacaaat acttttcctc
     1801 aacctaaaaa taaggcaatt agccaaaaac aactttgcgt gtaaacaacg ctcaatacac
     1861 gtgtcatttt attattagct attgcttcac cgccttagct ttctcgtgac ctagtcgtcc
     1921 tcgtcttttc ttcttcttct tctataaaac aatacccaaa gagctcttct tcttcacaat
     1981 tcagatttca atttctcaaa atcttaaaaa ctttctctca attctctcta ccgtgatcaa
     2041 ggtaaatttc tgtgttcctt attctctcaa aatcttcgat tttgttttcg ttcgatccca
     2101 atttcgtata tgttctttgg tttagattct gttaatctta gatcgaagac gattttctgg
     2161 gtttgatcgt tagatatcat cttaattctc gattagggtt tcatagatat catccgattt
     2221 gttcaaataa tttgagtttt gtcgaataat tactcttcga tttgtgattt ctatctagat
     2281 ctggtgttag tttctagttt gtgcgatcga atttgtcgat taatctgagt ttttctgatt
     2341 aacagaaggg cgaattcgac ccaagtttgt acaaaaaagc aggctccgcg gccgcatggg
     2401 caagcccatc cccaaccccc tgctgggcct ggacagcacc gctagcatcc cgctgctgaa
     2461 cgctaaacag attctgggac agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga
     2521 ctccaccaat cagtacctgc tggatcgaat cggcgagctg aagagtgggg atgcttgcat
     2581 tgcagaatat cagcaggcag ggagaggaag cagagggagg aaatggttct ctccttttgg
     2641 agctaacctg tacctgagta tgttttggcg cctgaagcgg ggaccagcag caatcggcct
     2701 gggcccggtc atcggaattg tcatggcaga agcgctgcga aagctgggag cagacaaggt
     2761 gcgagtcaaa tggcccaatg acctgtatct gcaggataga aagctggcag gcatcctggt
     2821 ggagctggcc ggaataacag gcgatgctgc acagatcgtc attggcgccg ggattaacgt
     2881 ggctatgagg cgcgtggagg aaagcgtggt caatcagggc tggatcacac tgcaggaagc
     2941 agggattaac ctggacagga atactctggc cgctatgctg atccgagagc tgcgggcagc
     3001 cctggaactg ttcgagcagg aaggcctggc tccatatctg tcacggtggg agaagctgga
     3061 taacttcatc aatagacccg tgaagctgat cattggggac aaagagattt tcgggattag
     3121 ccgggggatt gataaacagg gagccctgct gctggaacag gacggagtta tcaaaccctg
     3181 gatgggcgga gaaatcagtc tgcggtctgc cgaaaagctg cagctgcctc ccctggagcg
     3241 cctgaccctg gacggaggcg gtggaagcgg cggaggttcc atggtgagca agggcgagga
     3301 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3361 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     3421 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
     3481 cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     3541 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     3601 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     3661 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     3721 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     3781 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     3841 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     3901 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     3961 cgccgggatc actctcggca tggacgagct gtacaagtaa gggcccaagg gtgggcgcgc
     4021 cgacccagct ttcttgtaca aagtggttga taacagcgct tagagctcga atttccccga
     4081 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     4141 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     4201 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     4261 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     4321 gttactagat cgggaattgg ttccggaacc aattcgtaat catgtcatag ctgtttcctg
     4381 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     4441 aagcctgggg tgcctaatga gtgagctaac tcacattagg ctgaattagg cgcgcctatt
     4501 tctattaatt cccgatctag taacatagat gacaccgcgc gcgataattt atcctagttt
     4561 gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata
     4621 aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa
     4681 ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa
     4741 ctttattgcc aaatgtttga acgatcgggg aaattcgagc tggggacgtc cccagctcgg
     4801 taccccctcc tgggcacggt cgtacgcgat ccgccgcggt tccacctcac cattgcgggt
     4861 atgccagttg agttcgatga gttcccgtac cagctccaac tcgggcgatg ccacgcgcac
     4921 tccgttcaga tctcggtgac gggcaggacc ggacggggcg gtaccggcag gctgaagtcc
     4981 agctgccaga aacccacgtc atgccagttc ccgtgcttga agccggccgc ccgcagcatg
     5041 ccgcgggggg catatccgag cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg
     5101 atgacagcga ccacgctctt gaagccctgt gcctccaggg acttcagcag gtgggtgtag
     5161 agcgtggagc ccagtcccgt ccgctggtgg cggggggaga cgtacacggt cgactcggcc
     5221 gtccagtcgt aggcgttgcg tgccttccag gggcccgcgt aggcgatgcc ggcgacctcg
     5281 ccgtccacct cggcgacgag ccagggatag cgctcccgca gacggacgag gtcgtccgtc
     5341 cactcctgcg gttcctgcgg ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg
     5401 ttgacgatgg tgcagaccgc cggcatgtcc gcctcggtgg cacggcggat gtcggccggg
     5461 cgtcgttctg ggctcatatc ttattgcccc ccgggatcag atctggattg agagtgaata
     5521 tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa
     5581 gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct
     5641 gacgtatgtg cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt
     5701 tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata
     5761 acgtgactcc cttaattctc cgctcatgat cttgatcccc tgcgccatca gatccttggc
     5821 ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca
     5881 gctggcaatt ccggttcgct tgctgtccat aaaaccgccc agtctagcta tcgccatgta
     5941 agcccactgc aagctacctg ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc
     6001 cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtgttc
     6061 cgcttccttt agcagccctt gcgccctgag tgcttgcggc agcgtgaagc tgatccgtcg
     6121 agattaatag aaataaattc agcctaattc ggcgttaatt cagtacatta aaaacgtccg
     6181 caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc
     6241 agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag
     6301 cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat
     6361 gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc
     6421 tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaattc
     6481 gggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
     6541 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     6601 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
     6661 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     6721 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg gtgatcgccg
     6781 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     6841 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     6901 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     6961 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     7021 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     7081 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     7141 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     7201 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     7261 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     7321 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     7381 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     7441 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     7501 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctagcta
     7561 gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact
     7621 aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc
     7681 gtgcataata agccctacac aaattgggag atatatcatg catgaccaaa atcccttaac
     7741 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7801 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7861 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7921 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     7981 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8041 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8101 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8161 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8221 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8281 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8341 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8401 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8461 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8521 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     8581 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     8641 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     8701 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     8761 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     8821 ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga
     8881 gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt
     8941 ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg
     9001 accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac
     9061 aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc
     9121 cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg
     9181 ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc
     9241 tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca
     9301 tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca
     9361 tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat
     9421 ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac
     9481 tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg
     9541 ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg
     9601 tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga
     9661 tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg
     9721 cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg
     9781 caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc
     9841 catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg
     9901 gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca
     9961 tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta
    10021 cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca
    10081 gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca
    10141 tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac
    10201 cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt
    10261 caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact
    10321 tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac
    10381 cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac
    10441 ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc
    10501 ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact
    10561 catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg
    10621 taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca
    10681 actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct
    10741 tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct
    10801 ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc
    10861 gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc
    10921 tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag
    10981 cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc
    11041 cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc
    11101 ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt
    11161 gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg
    11221 ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag
    11281 cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac
    11341 taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt
    11401 cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt
    11461 atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac
    11521 tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc
    11581 ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt
    11641 gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct
    11701 tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt
    11761 ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg
    11821 caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca
    11881 gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt
    11941 cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact
    12001 tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac
    12061 gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg
    12121 tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg
    12181 caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa
    12241 cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc
    12301 cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt
    12361 tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc
    12421 tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa
    12481 aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg
    12541 acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac
    12601 tatagcagcg gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc
    12661 atgcacatac aaatggacga acggataaac ct
//
