LOCUS       sequence-243670-       12680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 
            promoter in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12680)
  AUTHORS   Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC
  TITLE     Proximity labeling of protein complexes and cell type-specific 
            organellar proteomes in Arabidopsis enabled by TurboID.
  JOURNAL   Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864.
  PUBMED    31535972
REFERENCE   2  (bases 1 to 12680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-19"; volume: "8. pii: 47864. doi"; pages: " 
            10.7554/eLife.47864"
COMMENT     Sequence Label: R4pGWB601_UBQ10p-miniTurbo-YFP-NLS
FEATURES             Location/Qualifiers
     source          1..12680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    322..342
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2361..2385
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2399..2440
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2447..3217
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             join(3245..3247,3248..3250,3251..3961)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3551..3570)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3898..3919
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3971..3991
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(4011..4035)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4067..4319
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4352..4368)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4352..4368)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4365..4387)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4376..4392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4400..4406,4407..4424,4425..4430))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4445..4466
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4502..4754)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4914..5465)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5517..5696)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6202..6226
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6747..7538
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7784..8372
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8273..8292
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8526..8543
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8558..8698
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8624..8643)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8784..8806
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9042..9236
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9302..10375)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10804..11433)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgtgg atcccccatc acaactttgt atagaaaagt tggctccgaa ttcgccctta
      361 gtctagctca acagagcttt taacccaaat tggtacaata gaatacaact ttagatcata
      421 attctcaaaa gaaagagatt ccttagctat tctatctgcc actccatttc cttctcggct
      481 tgtatgcaca agcataaaat cctcaaactt gctaagtaga tactttatgt cttggataat
      541 tggattgaga cttgacaagc ataactttca tgtaaccaaa gacacaagtt gctgagaatc
      601 cacctcaaaa atgatcttcc tataattgaa tcgggataat gacagcacag cccatctaag
      661 agcctccact tctacttcca gcacgcttct tacttttacc acagctcttg cacctaacca
      721 taacaccttc cctgtatgat cgcgaagcac ccaccctaag ccacatttta atccttctgt
      781 tggccatgcc ccatcaaagt tgcacttaac ccaagattgt ggtggagctt cccatgtttc
      841 tcgtctgtcc cgacggtgtt gtggttggtg ctttccttac attctgagcc tctttccttc
      901 taatccactc atctgcatct tcttgtgtcc ttactaatac ctcattggtt ccaaattccc
      961 tccctttaag caccagctcg tttctgttct tccacagcct cccaagtatc caagggacta
     1021 aagcctccac attcttcaga tcaggatatt cttgtttaag atgttgaact ctatggaggt
     1081 ttgtatgaac tgatgatcta ggaccggata agttcccttc ttcatagcga acttattcaa
     1141 agaatgtttt gtgtatcatt cttgttacat tgttattaat gaaaaaatat tattggtcat
     1201 tggactgaac acgagtgtta aatatggacc aggccccaaa taagatccat tgatatatga
     1261 attaaataac aagaataaat cgagtcacca aaccacttgc cttttttaac gagacttgtt
     1321 caccaacttg atacaaaagt cattatccta tgcaaatcaa taatcataca aaaatatcca
     1381 ataacactaa aaaattaaaa gaaatggata atttcacaat atgttatacg ataaagaagt
     1441 tacttttcca agaaattcac tgattttata agcccacttg cattagataa atggcaaaaa
     1501 aaaacaaaaa ggaaaagaaa taaagcacga agaattctag aaaatacgaa atacgcttca
     1561 atgcagtggg acccacggtt caattattgc caattttcag ctccaccgta tatttaaaaa
     1621 ataaaacgat aatgctaaaa aaatataaat cgtaacgatc gttaaatctc aacggctgga
     1681 tcttatgacg accgttagaa attgtggttg tcgacgagtc agtaataaac ggcgtcaaag
     1741 tggttgcagc cggcacacac gagtcgtgtt tatcaactca aagcacaaat acttttcctc
     1801 aacctaaaaa taaggcaatt agccaaaaac aactttgcgt gtaaacaacg ctcaatacac
     1861 gtgtcatttt attattagct attgcttcac cgccttagct ttctcgtgac ctagtcgtcc
     1921 tcgtcttttc ttcttcttct tctataaaac aatacccaaa gagctcttct tcttcacaat
     1981 tcagatttca atttctcaaa atcttaaaaa ctttctctca attctctcta ccgtgatcaa
     2041 ggtaaatttc tgtgttcctt attctctcaa aatcttcgat tttgttttcg ttcgatccca
     2101 atttcgtata tgttctttgg tttagattct gttaatctta gatcgaagac gattttctgg
     2161 gtttgatcgt tagatatcat cttaattctc gattagggtt tcatagatat catccgattt
     2221 gttcaaataa tttgagtttt gtcgaataat tactcttcga tttgtgattt ctatctagat
     2281 ctggtgttag tttctagttt gtgcgatcga atttgtcgat taatctgagt ttttctgatt
     2341 aacagaaggg cgaattcgac ccaagtttgt acaaaaaagc aggctccgcg gccgcatggg
     2401 caagcccatc cccaaccccc tgctgggcct ggacagcacc gctagcatcc cgctgctgaa
     2461 cgctaaacag attctgggac agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga
     2521 ctccaccaat cagtacctgc tggatcgaat cggcgagctg aagagtgggg atgcttgcat
     2581 tgcagaatat cagcaggcag ggagaggaag cagagggagg aaatggttct ctccttttgg
     2641 agctaacctg tacctgagta tgttttggcg cctgaagcgg ggaccagcag caatcggcct
     2701 gggcccggtc atcggaattg tcatggcaga agcgctgcga aagctgggag cagacaaggt
     2761 gcgagtcaaa tggcccaatg acctgtatct gcaggataga aagctggcag gcatcctggt
     2821 ggagctggcc ggaataacag gcgatgctgc acagatcgtc attggcgccg ggattaacgt
     2881 ggctatgagg cgcgtggagg aaagcgtggt caatcagggc tggatcacac tgcaggaagc
     2941 agggattaac ctggacagga atactctggc cgctatgctg atccgagagc tgcgggcagc
     3001 cctggaactg ttcgagcagg aaggcctggc tccatatctg tcacggtggg agaagctgga
     3061 taacttcatc aatagacccg tgaagctgat cattggggac aaagagattt tcgggattag
     3121 ccgggggatt gataaacagg gagccctgct gctggaacag gacggagtta tcaaaccctg
     3181 gatgggcgga gaaatcagtc tgcggtctgc cgaaaaggga ggcggtggaa gcggcggagg
     3241 ttccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     3301 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     3361 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     3421 caccctcgtg accaccttcg gctacggcct gcagtgcttc gcccgctacc ccgaccacat
     3481 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     3541 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     3601 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     3661 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     3721 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     3781 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3841 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3901 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3961 ggcttcggat ccaaaaaaga agagaaaggt atagaagggt gggcgcgccg acccagcttt
     4021 cttgtacaaa gtggttgata acagcgctta gagctcgaat ttccccgatc gttcaaacat
     4081 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     4141 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     4201 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     4261 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     4321 ggaattggtt ccggaaccaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
     4381 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     4441 cctaatgagt gagctaactc acattaggct gaattaggcg cgcctatttc tattaattcc
     4501 cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
     4561 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
     4621 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
     4681 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
     4741 atgtttgaac gatcggggaa attcgagctg gggacgtccc cagctcggta ccccctcctg
     4801 ggcacggtcg tacgcgatcc gccgcggttc cacctcacca ttgcgggtat gccagttgag
     4861 ttcgatgagt tcccgtacca gctccaactc gggcgatgcc acgcgcactc cgttcagatc
     4921 tcggtgacgg gcaggaccgg acggggcggt accggcaggc tgaagtccag ctgccagaaa
     4981 cccacgtcat gccagttccc gtgcttgaag ccggccgccc gcagcatgcc gcggggggca
     5041 tatccgagcg cctcgtgcat gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc
     5101 acgctcttga agccctgtgc ctccagggac ttcagcaggt gggtgtagag cgtggagccc
     5161 agtcccgtcc gctggtggcg gggggagacg tacacggtcg actcggccgt ccagtcgtag
     5221 gcgttgcgtg ccttccaggg gcccgcgtag gcgatgccgg cgacctcgcc gtccacctcg
     5281 gcgacgagcc agggatagcg ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt
     5341 tcctgcggct cggtacggaa gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg
     5401 cagaccgccg gcatgtccgc ctcggtggca cggcggatgt cggccgggcg tcgttctggg
     5461 ctcatatctt attgcccccc gggatcagat ctggattgag agtgaatatg agactctaat
     5521 tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct
     5581 agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct
     5641 tagctcatta aactccagaa acccgcggct gagtggctcc ttcaacgttg cggttctgtc
     5701 agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct
     5761 taattctccg ctcatgatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc
     5821 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
     5881 ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa
     5941 gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca
     6001 ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag
     6061 cagcccttgc gccctgagtg cttgcggcag cgtgaagctg atccgtcgag attaatagaa
     6121 ataaattcag cctaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt
     6181 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca
     6241 gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
     6301 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
     6361 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
     6421 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc
     6481 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     6541 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     6601 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     6661 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     6721 gtcgccctaa aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc
     6781 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     6841 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     6901 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     6961 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     7021 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     7081 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     7141 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     7201 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     7261 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     7321 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     7381 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     7441 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     7501 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca
     7561 agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt
     7621 gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag
     7681 ccctacacaa attgggagat atatcatgca tgaccaaaat cccttaacgt gagttttcgt
     7741 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7801 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7861 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7921 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7981 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8041 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8101 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8161 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8221 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8281 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8341 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8401 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8461 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8521 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     8581 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     8641 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     8701 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     8761 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     8821 accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg
     8881 cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag
     8941 cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag
     9001 gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg
     9061 ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt
     9121 ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt
     9181 acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa
     9241 agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac
     9301 attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg
     9361 ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca
     9421 gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga
     9481 tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga
     9541 gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg
     9601 ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact
     9661 ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg
     9721 ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg
     9781 tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc
     9841 ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct
     9901 tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt
     9961 cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg
    10021 gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg
    10081 ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa
    10141 aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg
    10201 gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg
    10261 cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt
    10321 catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga
    10381 ttcctcgggc ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac
    10441 gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg
    10501 ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt
    10561 tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc
    10621 cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga
    10681 cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg
    10741 cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt
    10801 aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc
    10861 gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac
    10921 gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac
    10981 ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct
    11041 tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat
    11101 gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac
    11161 agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc
    11221 cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc
    11281 ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc
    11341 ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc
    11401 gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt
    11461 actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat
    11521 caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc
    11581 agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg
    11641 ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa
    11701 aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct
    11761 cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc
    11821 tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat
    11881 gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc
    11941 gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg
    12001 ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg
    12061 caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg
    12121 gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta
    12181 ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc
    12241 gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct
    12301 cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct
    12361 ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg
    12421 cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa
    12481 gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca
    12541 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
    12601 ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa
    12661 atggacgaac ggataaacct
//
