LOCUS       sequence-243686-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Beilina A, Van Der Brug M, Ahmad R, Kesavapany S, Miller DW, Petsko 
            GA, Cookson MR
  TITLE     Mutations in PTEN-induced putative kinase 1 associated with 
            recessive parkinsonism have differential effects on protein 
            stability.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Apr 19. 102(16):5703-8.
  PUBMED    15824318
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Apr 19. 102(16):5703-8."
COMMENT     Sequence Label: pLenti6-DEST PINK1-V5 WT
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     59..76
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    193..214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(229..234,235..252,253..259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    267..283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     272..294
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     291..307
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     291..307
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     326..346
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        328..346
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        374..600
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             601..781
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    828..953
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1446..1679
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1864..1908
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2187..2490
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2491..2694
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2645..2665
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2691..2715
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2811..2835
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2853..4595
                     /codon_start=1
                     /product="human PTEN-induced putative kinase 1, a 
                     mitochondria serine/threonine protein kinase"
                     /label=PINK1
                     /translation="MAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGER
                     PGWAAGPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAVFLAF
                     GLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIG
                     KGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIKMMW
                     NISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAF
                     TSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAA
                     MMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLP
                     FSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGK
                     AHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHIL
                     ALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCS
                     WRAAL"
     protein_bind    complement(4615..4639)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4692..4733
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4770..4790)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4775..5104
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4955..5090
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5152..5199
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5218..5616
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5703..5936
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6008..6129
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6045..6064)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6099..6118
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6169..6304
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6324..6343)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6325..6343)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6353..6370)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6353..6369)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6362..6384)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6511..6966
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6598..6617)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6808..6829
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6992..7096
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7097..7165,7166..7957)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7315..7334)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8128..8716
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8617..8636
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
       61 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
      121 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      181 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      241 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
      301 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
      361 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
      421 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
      481 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
      541 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
      601 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
      661 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      721 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      781 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      841 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      901 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      961 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     1021 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     1081 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     1141 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     1201 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     1261 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     1321 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1381 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1441 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1501 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1561 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1621 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1681 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1741 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1801 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1861 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1921 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1981 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2041 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2101 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     2161 cggtatcgat aagcttggga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2221 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2281 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2341 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2401 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2461 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2521 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2581 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2641 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     2701 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     2761 cgactctaga ggatccacta gtccagtgtg gtggaattct gcagatatca acaagtttgt
     2821 acaaaaaagc aggcttgaag gaattcggta ccatggcggt gcgacaggcg ctgggccgcg
     2881 gcctgcagct gggtcgagcg ctgctgctgc gcttcacggg caagcccggc cgggcctacg
     2941 gcttggggcg gccgggcccg gcggcgggct gtgtccgcgg ggagcgtcca ggctgggccg
     3001 caggaccggg cgcggagcct cgcagggtcg ggctcgggct ccctaaccgt ctccgcttct
     3061 tccgccagtc ggtggccggg ctggcggcgc ggttgcagcg gcagttcgtg gtgcgggcct
     3121 ggggctgcgc gggcccttgc ggccgggcag tctttctggc cttcgggcta gggctgggcc
     3181 tcatcgagga aaaacaggcg gagagccggc gggcggtctc ggcctgtcag gagatccagg
     3241 caatttttac ccagaaaagc aagccggggc ctgacccgtt ggacacgaga cgcttgcagg
     3301 gctttcggct ggaggagtat ctgatagggc agtccattgg taagggctgc agtgctgctg
     3361 tgtatgaagc caccatgcct acattgcccc agaacctgga ggtgacaaag agcaccgggt
     3421 tgcttccagg gagaggccca ggtaccagtg caccaggaga agggcaggag cgagctccgg
     3481 gggcccctgc cttccccttg gccatcaaga tgatgtggaa catctcggca ggttcctcca
     3541 gcgaagccat cttgaacaca atgagccagg agctggtccc agcgagccga gtggccttgg
     3601 ctggggagta tggagcagtc acttacagaa aatccaagag aggtcccaag caactagccc
     3661 ctcaccccaa catcatccgg gttctccgcg ccttcacctc ttccgtgccg ctgctgccag
     3721 gggccctggt cgactaccct gatgtgctgc cctcacgcct ccaccctgaa ggcctgggcc
     3781 atggccggac gctgttcctc gttatgaaga actatccctg taccctgcgc cagtaccttt
     3841 gtgtgaacac acccagcccc cgcctcgccg ccatgatgct gctgcagctg ctggaaggcg
     3901 tggaccatct ggttcaacag ggcatcgcgc acagagacct gaaatccgac aacatccttg
     3961 tggagctgga cccagacggc tgcccctggc tggtgatcgc agattttggc tgctgcctgg
     4021 ctgatgagag catcggcctg cagttgccct tcagcagctg gtacgtggat cggggcggaa
     4081 acggctgtct gatggcccca gaggtgtcca cggcccgtcc tggccccagg gcagtgattg
     4141 actacagcaa ggctgatgcc tgggcagtgg gagccatcgc ctatgaaatc ttcgggcttg
     4201 tcaatccctt ctacggccag ggcaaggccc accttgaaag ccgcagctac caagaggctc
     4261 agctacctgc actgcccgag tcagtgcctc cagacgtgag acagttggtg agggcactgc
     4321 tccagcgaga ggccagcaag agaccatctg cccgagtagc cgcaaatgtg cttcatctaa
     4381 gcctctgggg tgaacatatt ctagccctga agaatctgaa gttagacaag atggttggct
     4441 ggctcctcca acaatcggcc gccactttgt tggccaacag gctcacagag aagtgttgtg
     4501 tggaaacaaa aatgaagatg ctctttctgg ctaacctgga gtgtgaaacg ctctgccagg
     4561 cagccctcct cctctgctca tggagggcag ccctgaagct cgagtgcggc cgcaacccag
     4621 ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc
     4681 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     4741 gttagtaatg agtttggaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4801 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4861 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4921 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4981 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5041 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5101 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     5161 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     5221 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
     5281 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
     5341 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
     5401 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
     5461 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
     5521 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
     5581 gaattgctgc cctctggtta tgtgtgggag ggctaagcac aattcgagct cggtaccttt
     5641 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5701 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     5761 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5821 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5881 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     5941 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6001 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6061 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6121 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     6181 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6241 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6301 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     6361 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     6421 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6481 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     6541 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6601 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6661 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6721 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6781 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6841 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6901 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6961 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7021 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7081 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7141 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7201 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7261 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7321 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7381 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7441 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7501 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7561 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7621 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7681 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7741 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7801 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7861 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7921 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7981 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8041 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8101 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8161 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8221 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8281 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8341 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8401 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8461 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8521 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8581 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8641 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8701 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8761 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata a
//
