LOCUS       sequence-243690-        9638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses flippase fused with destabilizing domain (DD) downstream 
            of a 10XUAS sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Sethi S, Wang JW
  TITLE     A versatile genetic tool for post-translational control of gene 
            expression in Drosophila melanogaster.
  JOURNAL   Elife. 2017 Nov 15;6. pii: e30327. doi: 10.7554/eLife.30327.
  PUBMED    29140243
REFERENCE   2  (bases 1 to 9638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-11-15"; volume: "6. pii: e30327. doi"; pages: " 
            10.7554/eLife.30327"
COMMENT     Sequence Label: 10XUAS-flp-DD
FEATURES             Location/Qualifiers
     source          1..9638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    263..357
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    391..485
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        516..754
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     701..722
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          769..835
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             879..2147
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFLSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     primer_bind     complement(3339..3357)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3424..3528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3529..3597,3598..4389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3747..3766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4560..5148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5049..5068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5235..5304
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(5529..5547)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5999..6070,6472..6745,6820..7474,7536..7851,8055..8186,
                     8257..8871)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 gggcgcgcct ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg
       61 aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc
      121 agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc
      181 gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccagggcc
      241 gcaagcttgc atgcctgcag gtcggagtac tgtcctccga gcggagtact gtcctccgag
      301 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
      361 agactctagc cctagggcat gcctgcaggt cggagtactg tcctccgagc ggagtactgt
      421 cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct
      481 ccgagcggag actctagcgc tagcgacgtc gagcgccgga gtataaatag aggcgcttcg
      541 tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta
      601 agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagtaaagtg caagttaaag
      661 tgaatcaatt aaaagtaacc agcaaccaag taaatcaact gcaactactg aaatctgcca
      721 agaagtaatt attgaataca agaagagaac tctgaataga tctaaaaggt aggttcaacc
      781 actgatgcct aggcacaccg aaacgactaa ccctaattct tatcctttac ttcaggcggc
      841 cgcggctgga gggtaccaac ttaaaaaaaa aaatcaaaat gccacaattt gatatattat
      901 gtaaaacacc acctaaggtg cttgttcgtc agtttgtgga aaggtttgaa agaccttcag
      961 gtgagaaaat agcattatgt gctgctgaac taacctattt atgttggatg attacacata
     1021 acggaacagc aatcaagaga gccacattca tgagctataa tactatcata agcaattcgc
     1081 tgagtttcga tattgtcaat aaatcactcc agtttaaata caagacgcaa aaagcaacaa
     1141 ttctggaagc ctcattaaag aaattgattc ctgcttggga atttacaatt attccttact
     1201 atggacaaaa acatcaatct gatatcactg atattgtaag tagtttgcaa ttacagttcg
     1261 aatcatcgga agaagcagat aagggaaata gccacagtaa aaaaatgctt aaagcacttc
     1321 taagtgaggg tgaaagcatc tgggagatca ctgagaaaat actaaattcg tttgagtata
     1381 cttcgagatt tacaaaaaca aaaactttat accaattcct cttcctagct actttcatca
     1441 attgtggaag attcagcgat attaagaacg ttgatccgaa atcatttaaa ttagtccaaa
     1501 ataagtatct gggagtaata atccagtgtt tagtgacaga gacaaagaca agcgttagta
     1561 ggcacatata cttccttagc gcaaggggta ggatcgatcc acttgtatat ttggatgaat
     1621 ttttgaggaa ttctgaacca gtcctaaaac gagtaaatag gaccggcaat tcttcaagca
     1681 ataaacagga ataccaatta ttaaaagata acttagtcag atcgtacaat aaagctttga
     1741 agaaaaatgc gccttattca atctttgcta taaaaaatgg cccaaaatct cacattggaa
     1801 gacatttgat gacctcattt ctttcaatga agggcctaac ggagttgact aatgttgtgg
     1861 gaaattggag cgataagcgt gcttctgccg tggccaggac aacgtatact catcagataa
     1921 cagcaatacc tgatcactac ttcgcactag tttctcggta ctatgcatat gatccaatat
     1981 caaaggaaat gatagcattg aaggatgaga ctaatccaat tgaggagtgg cagcatatag
     2041 aacagctaaa gggtagtgct gaaggaagca tacgataccc cgcatggaat gggataatat
     2101 cacaggaggt actagactac ctttcatcct acataaatag acgcatactc gagatctccc
     2161 tgattgccgc cctggccgtg gaccacgtga ttggcatgga aaatgccatg ccctggaacc
     2221 tgcccgccga tctggcctgg ttcaagcgca acaccttgga caagcccgtg atcatgggcc
     2281 gccacacttg ggaaagcatc ggtcgtccct tgcccggccg caaggatatc attctgagca
     2341 gccagccgtc caccgacgat cgcgtcactt gggtgaagag cgtcgacgag gccatcgctg
     2401 cctgcggaga tgtcccggag atcatggtga tcggaggagg acgtgtctac gagcagttcc
     2461 tgcccaaggc ccagaagctc tacctgaccc atatcgatgc cgaggtggag ggagataccc
     2521 acttccccga ctacgagccc gacggttggg agtcggtgtt ctcggagttc cacgacgccg
     2581 acgcccagaa ctcgcactcg tactgcttcg agattctgga gcgccgttaa tctagaatga
     2641 atcgttttta aaataacaaa tcaattgttt tataatattc gtacgattct ttgattatgt
     2701 aataaaatgt gatcattagg aagattacga aaaatataaa aaatatgagt tctgtgtgta
     2761 taacaaatgc tgtaaacgcc acaattgtgt ttgttgcaaa taaacccatg attatttgat
     2821 taaaattgtt gttttctttg ttcatagaca atagtgtgtt ttgcctaaac gtgtactgca
     2881 taaactccat gcgagtgtat agcgagctag tggctaacgc ttgccccacc aaagtagatt
     2941 cgtcaaaatc ctcaatttca tcaccctcct ccaagtttaa catttggccg tcggaattaa
     3001 cttctaaaga tgccacataa tctaataaat gaaatagaga ttcaaacgtg gcgtcatcgt
     3061 ccgtttcgac catttccgaa aagaactcgg gcataaactc tatgatttct ctggacgtgg
     3121 tgttgtcgaa actctcaaag tacgcagtca ggaacgtgcg cgacatgtcg tcgggaaact
     3181 cgcgcggaaa catgttgttg taaccgaacg ggtcccatag cgccaaaacc aaatctgcca
     3241 gcgtcaatag aatgagcacg atgccgacaa tggagctggc ttggatagcg attcgagtta
     3301 acggccggcc gtttaaacga attcttgaag acgaaagggc ctcgtgatac gcctattttt
     3361 ataggttaat gtcatgataa taatggtttc ttagactcag gtggcacttt tcggggaaat
     3421 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3481 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3541 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3601 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3661 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3721 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     3781 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     3841 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     3901 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3961 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4021 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4081 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4141 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4201 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4261 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4321 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4381 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4441 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4501 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4561 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4621 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4681 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4741 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4801 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4861 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4921 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4981 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5041 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5101 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5161 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt accgtcgacg
     5221 atgtaggtca cggtctcgaa gccgcggtgc gggtgccagg gcgtgccctt gggctccccg
     5281 ggcgcgtact ccacctcacc catctggtcc atcatgatga acgggtcgag gtggcggtag
     5341 ttgatcccgg cgaacgcgcg gcgcaccggg aagccctcgc cctcgaaacc gctgggcgcg
     5401 gtggtcacgg tgagcacggg acgtgcgacg gcgtcggcgg gtgcggatac gcggggcagc
     5461 gtcagcgggt tctcgacggt cacggcgggc atgtcgacaa gccgaacata tgggcgcgcc
     5521 tagtatgtat gtaagttaat aaaacccatt tttgcggaaa gtagataaaa aaaacatttt
     5581 ttttttttac tgcactggat atcattgaac ttatctgatc agttttaaat ttacttcgat
     5641 ccaagggtat ttgatgtacc aggttctttc gattacctct cactcaaaat gacattccac
     5701 tcaaagtcag cgctgtttgc ctccttctct gtccacagaa atatcgccgt ctctttcgcc
     5761 gctgcgtccg ctatctcttt cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct
     5821 tcagttgcat tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg ccatcaatta
     5881 aacacaaagt gctgtgccaa aactcctctc gcttcttatt tttgtttgtt ttttgagtga
     5941 ttggggtggt gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat
     6001 gggccaagag gatcaggagc tattaattcg cggaggcagc aaacacccat ctgccgagca
     6061 tctgaacaat gtgagtagta catgtgcata catcttaagt tcacttgatc tataggaact
     6121 gcgattgcaa catcaaattg tctgcggcgt gagaactgcg acccacaaaa atcccaaacc
     6181 gcaattgcac aaacaaatag tgacacgaaa cagattattc tggtagctgt tctcgctata
     6241 taagacaatt tttgagatca tatcatgatc aagacatcta aaggcattca ttttcgacta
     6301 tattcttttt tacaaaaaat ataacaacca gatattttaa gctgatccta gatgcacaaa
     6361 aaataaataa aagtataaac ctacttcgta ggatacttcg gggtactttt tgttcggggt
     6421 tagatgagca taacgcttgt agttgatatt tgagatcccc tatcattgca gggtgacagc
     6481 ggagcggctt cgcagagctg cattaaccag ggcttcgggc aggccaaaaa ctacggcacg
     6541 ctccggccac ccagtccgcc ggaggactcc ggttcaggga gcggccaact agccgagaac
     6601 ctcacctatg cctggcacaa tatggacatc tttggggcgg tcaatcagcc gggctccgga
     6661 tggcggcagc tggtcaaccg gacacgcgga ctattctgca acgagcgaca cataccggcg
     6721 cccaggaaac atttgctcaa gaacggtgag tttctattcg cagtcggctg atctgtgtga
     6781 aatcttaata aagggtccaa ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg
     6841 gcgaactttt ggccgtgatg ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc
     6901 ttgcctttcg atcgccgcag ggcatccaag tatcgccatc cgggatgcga ctgctcaatg
     6961 gccaacctgt ggacgccaag gagatgcagg ccaggtgcgc ctatgtccag caggatgacc
     7021 tctttatcgg ctccctaacg gccagggaac acctgatttt ccaagccatg gtgcggatgc
     7081 cacgacatct gacctatcgg cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt
     7141 cgctcagcaa atgtcagcac acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg
     7201 gcggagaaag gaagcgtctg gcattcgcct ccgaggctct aaccgatccg ccgcttctga
     7261 tctgcgatga gcccacctcc ggactggact cctttaccgc ccacagcgtc gtccaggtgc
     7321 tgaagaagct gtcgcagaag ggcaagaccg tcatcctgac cattcatcag ccgtcttccg
     7381 agctgtttga gctctttgac aagatccttc tgatggccga gggcagggta gctttcttgg
     7441 gcactcccag cgaagccgtc gacttctttt cctagtgagt tcgatgtgtt tattaagggt
     7501 atctagtatt acataacatc tcaactccta tccagcgtgg gtgcccagtg tcctaccaac
     7561 tacaatccgg cggactttta cgtacaggtg ttggccgttg tgcccggacg ggagatcgag
     7621 tcccgtgatc ggatcgccaa gatatgcgac aattttgcca ttagcaaagt agcccgggat
     7681 atggagcagt tgttggccac caaaaatctg gagaagccac tggagcagcc ggagaatggg
     7741 tacacctaca aggccacctg gttcatgcag ttccgggcgg tcctgtggcg atcctggctg
     7801 tcggtgctca aggaaccact cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt
     7861 tccagtggaa acaaatgata taacgcttac aattcttgga aacaaattcg ctagatttta
     7921 gatagaattg cctgattcca cacccttctt agtttttttc aatgagatgt atagtttata
     7981 gttttgcaga agataaataa atttcattta actcgcgaat attaatgaga tgcgagtaac
     8041 attttaattt gcagatggtt gccatcttga ttggcctcat ctttttgggc caacaactca
     8101 cgcaagtggg tgtgatgaat atcaacggag ccatcttcct cttcctgacc aacatgacct
     8161 ttcaaaacgt ctttgccacg ataaatgtaa gtcatgttta gaatacattt gcatttcaat
     8221 aatttactaa ctttctaatg aatcgattcg atttaggtgt tcacctcaga gctgccagtt
     8281 tttatgaggg aggcccgaag tcgactttat cgctgtgaca catactttct gggcaaaacg
     8341 attgccgaat tgccgctttt tctcacagtg ccactggtct tcacggcgat tgcctatccg
     8401 atgatcggac tgcgggccgg agtgctgcac ttcttcaact gcctggcgct ggtcactctg
     8461 gtggccaatg tgtcaacgtc cttcggatat ctaatatcct gcgccagctc ctcgacctcg
     8521 atggcgctgt ctgtgggtcc gccggttatc ataccattcc tgctctttgg cggcttcttc
     8581 ttgaactcgg gctcggtgcc agtatacctc aaatggttgt cgtacctctc atggttccgt
     8641 tacgccaacg agggtctgct gattaaccaa tgggcggacg tggagccggg cgaaattagc
     8701 tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg tcatcctgga gacgcttaac
     8761 ttctccgccg ccgatctgcc gctggactac gtgggtctgg ccattctcat cgtgagcttc
     8821 cgggtgctcg catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat
     8881 atccgaaata actgcttgtt tttttttttt accattatta ccatcgtgtt tactgtttat
     8941 tgccccctca aaaagctaat gtaattatat ttgtgccaat aaaaacaaga tatgacctat
     9001 agaatacaag tatttcccct tcgaacatcc ccacaagtag actttggatt tgtcttctaa
     9061 ccaaaagact tacacacctg cataccttac atcaaaaact cgtttatcgc tacataaaac
     9121 accgggatat attttttata tacatacttt tcaaatcgcg cgccctcttc ataattcacc
     9181 tccaccacac cacgtttcgt agttgctctt tcgctgtctc ccacccgctc tccgcaacac
     9241 attcaccttt tgttcgacga ccttggagcg actgtcgtta gttccgcgcg attcggttcg
     9301 ctcaaatggt tccgagtggt tcatttcgtc tcaatagaaa ttagtaataa atatttgtat
     9361 gtacaattta tttgctccaa tatatttgta tatatttccc tcacagctat atttattcta
     9421 atttaatatt atgacttttt aaggtaattt tttgtgacct gttcggagtg attagcgtta
     9481 caatttgaac tgaaagtgac atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa
     9541 tggtgggcat aatagtgttg tttatatata tcaaaaataa caactataat aataagaata
     9601 catttaattt agaaaatgct tggatttcac tggaacta
//
