LOCUS       sequence-243692-        9083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP fused with destabilizing domain (DD) downstream of a 
            10XUAS sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Sethi S, Wang JW
  TITLE     A versatile genetic tool for post-translational control of gene 
            expression in Drosophila melanogaster.
  JOURNAL   Elife. 2017 Nov 15;6. pii: e30327. doi: 10.7554/eLife.30327.
  PUBMED    29140243
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-11-15"; volume: "6. pii: e30327. doi"; pages: " 
            10.7554/eLife.30327"
COMMENT     Sequence Label: 10xUAS-GFP-DD
FEATURES             Location/Qualifiers
     source          1..9083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    144..238
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    272..366
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        397..635
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     582..603
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          650..716
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             760..1473
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2665..2683)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2750..2854
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2855..2923,2924..3715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3073..3092)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3886..4474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4375..4394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4561..4630
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(4855..4873)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5325..5396,5798..6071,6146..6800,6862..7177,7381..7512,
                     7583..8197)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc
       61 cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccagggc
      121 cgcaagcttg catgcctgca ggtcggagta ctgtcctccg agcggagtac tgtcctccga
      181 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      241 gagactctag ccctagggca tgcctgcagg tcggagtact gtcctccgag cggagtactg
      301 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
      361 tccgagcgga gactctagcg ctagcgacgt cgagcgccgg agtataaata gaggcgcttc
      421 gtctacggag cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct
      481 aagcaaataa acaagcgcag ctgaacaagc taaacaatct gcagtaaagt gcaagttaaa
      541 gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac tgcaactact gaaatctgcc
      601 aagaagtaat tattgaatac aagaagagaa ctctgaatag atctaaaagg taggttcaac
      661 cactgatgcc taggcacacc gaaacgacta accctaattc ttatccttta cttcaggcgg
      721 ccgcggctcg agggtaccaa cttaaaaaaa aaaatcaaaa tgtccaaagg tgaagaactg
      781 tttaccggag tagtcccgat attggttgaa ctcgacggcg atgtcaacgg tcataaattc
      841 agtgtgtccg gcgagggtga gggcgacgcc acatacggta agctgacgtt gaagttcata
      901 tgcaccacgg gcaagctgcc cgtgccatgg ccgacgttgg tcacgacgct gacgtatggt
      961 gtccagtgtt tcagccgtta ccccgatcat atgaagcagc atgacttttt caagtcggcg
     1021 atgccggagg gatacgttca agagaggacc attttcttca aggatgacgg caactataag
     1081 acgcgagcgg aggtgaaatt tgaaggcgac acactcgtta accgtattga gttgaagggc
     1141 attgatttta aggaggatgg caacattctg ggccataagt tggaatacaa ctacaactcg
     1201 cataatgtgt atataatggc agataagcag aaaaacggaa taaaggttaa cttcaagatt
     1261 cgccacaaca tagaggacgg ttccgtgcaa cttgcagatc attaccaaca gaacacaccc
     1321 attggagatg gcccagttct cttgccagac aatcactacc tttccacaca gtccgcgttg
     1381 agcaaggacc ccaatgaaaa gcgggaccac atggtgttgc tggagtttgt gaccgcagct
     1441 ggtattacac acggcatgga tgagctctac aagctcgaga tctccctgat tgccgccctg
     1501 gccgtggacc acgtgattgg catggaaaat gccatgccct ggaacctgcc cgccgatctg
     1561 gcctggttca agcgcaacac cttggacaag cccgtgatca tgggccgcca cacttgggaa
     1621 agcatcggtc gtcccttgcc cggccgcaag gatatcattc tgagcagcca gccgtccacc
     1681 gacgatcgcg tcacttgggt gaagagcgtc gacgaggcca tcgctgcctg cggagatgtc
     1741 ccggagatca tggtgatcgg aggaggacgt gtctacgagc agttcctgcc caaggcccag
     1801 aagctctacc tgacccatat cgatgccgag gtggagggag atacccactt ccccgactac
     1861 gagcccgacg gttgggagtc ggtgttctcg gagttccacg acgccgacgc ccagaactcg
     1921 cactcgtact gcttcgagat tctggagcgc cgttaatcta gaatgaatcg tttttaaaat
     1981 aacaaatcaa ttgttttata atattcgtac gattctttga ttatgtaata aaatgtgatc
     2041 attaggaaga ttacgaaaaa tataaaaaat atgagttctg tgtgtataac aaatgctgta
     2101 aacgccacaa ttgtgtttgt tgcaaataaa cccatgatta tttgattaaa attgttgttt
     2161 tctttgttca tagacaatag tgtgttttgc ctaaacgtgt actgcataaa ctccatgcga
     2221 gtgtatagcg agctagtggc taacgcttgc cccaccaaag tagattcgtc aaaatcctca
     2281 atttcatcac cctcctccaa gtttaacatt tggccgtcgg aattaacttc taaagatgcc
     2341 acataatcta ataaatgaaa tagagattca aacgtggcgt catcgtccgt ttcgaccatt
     2401 tccgaaaaga actcgggcat aaactctatg atttctctgg acgtggtgtt gtcgaaactc
     2461 tcaaagtacg cagtcaggaa cgtgcgcgac atgtcgtcgg gaaactcgcg cggaaacatg
     2521 ttgttgtaac cgaacgggtc ccatagcgcc aaaaccaaat ctgccagcgt caatagaatg
     2581 agcacgatgc cgacaatgga gctggcttgg atagcgattc gagttaacgg ccggccgttt
     2641 aaacgaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     2701 tgataataat ggtttcttag actcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2761 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2821 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     2881 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     2941 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     3001 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     3061 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     3121 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     3181 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     3241 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     3301 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     3361 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     3421 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     3481 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     3541 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     3601 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     3661 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     3721 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3781 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3841 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3901 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3961 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4021 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4081 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4141 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4201 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4261 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4321 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4381 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4441 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4501 gttcctggcc ttttgctggc cttttgctca catgttaccg tcgacgatgt aggtcacggt
     4561 ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
     4621 ctcacccatc tggtccatca tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa
     4681 cgcgcggcgc accgggaagc cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag
     4741 cacgggacgt gcgacggcgt cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc
     4801 gacggtcacg gcgggcatgt cgacaagccg aacatatggg cgcgcctagt atgtatgtaa
     4861 gttaataaaa cccatttttg cggaaagtag ataaaaaaaa catttttttt ttttactgca
     4921 ctggatatca ttgaacttat ctgatcagtt ttaaatttac ttcgatccaa gggtatttga
     4981 tgtaccaggt tctttcgatt acctctcact caaaatgaca ttccactcaa agtcagcgct
     5041 gtttgcctcc ttctctgtcc acagaaatat cgccgtctct ttcgccgctg cgtccgctat
     5101 ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt ccgccttcag ttgcattttg
     5161 tcagcggttt cgtgacgaag ctccaagcgg tttacgccat caattaaaca caaagtgctg
     5221 tgccaaaact cctctcgctt cttatttttg tttgtttttt gagtgattgg ggtggtgatt
     5281 ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc ggcaatgggc caagaggatc
     5341 aggagctatt aattcgcgga ggcagcaaac acccatctgc cgagcatctg aacaatgtga
     5401 gtagtacatg tgcatacatc ttaagttcac ttgatctata ggaactgcga ttgcaacatc
     5461 aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc caaaccgcaa ttgcacaaac
     5521 aaatagtgac acgaaacaga ttattctggt agctgttctc gctatataag acaatttttg
     5581 agatcatatc atgatcaaga catctaaagg cattcatttt cgactatatt cttttttaca
     5641 aaaaatataa caaccagata ttttaagctg atcctagatg cacaaaaaat aaataaaagt
     5701 ataaacctac ttcgtaggat acttcggggt actttttgtt cggggttaga tgagcataac
     5761 gcttgtagtt gatatttgag atcccctatc attgcagggt gacagcggag cggcttcgca
     5821 gagctgcatt aaccagggct tcgggcaggc caaaaactac ggcacgctcc ggccacccag
     5881 tccgccggag gactccggtt cagggagcgg ccaactagcc gagaacctca cctatgcctg
     5941 gcacaatatg gacatctttg gggcggtcaa tcagccgggc tccggatggc ggcagctggt
     6001 caaccggaca cgcggactat tctgcaacga gcgacacata ccggcgccca ggaaacattt
     6061 gctcaagaac ggtgagtttc tattcgcagt cggctgatct gtgtgaaatc ttaataaagg
     6121 gtccaattac caatttgaaa ctcagtttgc ggcgtggcct atccgggcga acttttggcc
     6181 gtgatgggca gttccggtgc cggaaagacg accctgctga atgcccttgc ctttcgatcg
     6241 ccgcagggca tccaagtatc gccatccggg atgcgactgc tcaatggcca acctgtggac
     6301 gccaaggaga tgcaggccag gtgcgcctat gtccagcagg atgacctctt tatcggctcc
     6361 ctaacggcca gggaacacct gattttccaa gccatggtgc ggatgccacg acatctgacc
     6421 tatcggcagc gagtggcccg cgtggatcag gtgatccagg agctttcgct cagcaaatgt
     6481 cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc tgtccggcgg agaaaggaag
     6541 cgtctggcat tcgcctccga ggctctaacc gatccgccgc ttctgatctg cgatgagccc
     6601 acctccggac tggactcctt taccgcccac agcgtcgtcc aggtgctgaa gaagctgtcg
     6661 cagaagggca agaccgtcat cctgaccatt catcagccgt cttccgagct gtttgagctc
     6721 tttgacaaga tccttctgat ggccgagggc agggtagctt tcttgggcac tcccagcgaa
     6781 gccgtcgact tcttttccta gtgagttcga tgtgtttatt aagggtatct agtattacat
     6841 aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct accaactaca atccggcgga
     6901 cttttacgta caggtgttgg ccgttgtgcc cggacgggag atcgagtccc gtgatcggat
     6961 cgccaagata tgcgacaatt ttgccattag caaagtagcc cgggatatgg agcagttgtt
     7021 ggccaccaaa aatctggaga agccactgga gcagccggag aatgggtaca cctacaaggc
     7081 cacctggttc atgcagttcc gggcggtcct gtggcgatcc tggctgtcgg tgctcaagga
     7141 accactcctc gtaaaagtgc gacttattca gacaacggtg agtggttcca gtggaaacaa
     7201 atgatataac gcttacaatt cttggaaaca aattcgctag attttagata gaattgcctg
     7261 attccacacc cttcttagtt tttttcaatg agatgtatag tttatagttt tgcagaagat
     7321 aaataaattt catttaactc gcgaatatta atgagatgcg agtaacattt taatttgcag
     7381 atggttgcca tcttgattgg cctcatcttt ttgggccaac aactcacgca agtgggtgtg
     7441 atgaatatca acggagccat cttcctcttc ctgaccaaca tgacctttca aaacgtcttt
     7501 gccacgataa atgtaagtca tgtttagaat acatttgcat ttcaataatt tactaacttt
     7561 ctaatgaatc gattcgattt aggtgttcac ctcagagctg ccagttttta tgagggaggc
     7621 ccgaagtcga ctttatcgct gtgacacata ctttctgggc aaaacgattg ccgaattgcc
     7681 gctttttctc acagtgccac tggtcttcac ggcgattgcc tatccgatga tcggactgcg
     7741 ggccggagtg ctgcacttct tcaactgcct ggcgctggtc actctggtgg ccaatgtgtc
     7801 aacgtccttc ggatatctaa tatcctgcgc cagctcctcg acctcgatgg cgctgtctgt
     7861 gggtccgccg gttatcatac cattcctgct ctttggcggc ttcttcttga actcgggctc
     7921 ggtgccagta tacctcaaat ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg
     7981 tctgctgatt aaccaatggg cggacgtgga gccgggcgaa attagctgca catcgtcgaa
     8041 caccacgtgc cccagttcgg gcaaggtcat cctggagacg cttaacttct ccgccgccga
     8101 tctgccgctg gactacgtgg gtctggccat tctcatcgtg agcttccggg tgctcgcata
     8161 tctggctcta agacttcggg cccgacgcaa ggagtagccg acatatatcc gaaataactg
     8221 cttgtttttt ttttttacca ttattaccat cgtgtttact gtttattgcc ccctcaaaaa
     8281 gctaatgtaa ttatatttgt gccaataaaa acaagatatg acctatagaa tacaagtatt
     8341 tccccttcga acatccccac aagtagactt tggatttgtc ttctaaccaa aagacttaca
     8401 cacctgcata ccttacatca aaaactcgtt tatcgctaca taaaacaccg ggatatattt
     8461 tttatataca tacttttcaa atcgcgcgcc ctcttcataa ttcacctcca ccacaccacg
     8521 tttcgtagtt gctctttcgc tgtctcccac ccgctctccg caacacattc accttttgtt
     8581 cgacgacctt ggagcgactg tcgttagttc cgcgcgattc ggttcgctca aatggttccg
     8641 agtggttcat ttcgtctcaa tagaaattag taataaatat ttgtatgtac aatttatttg
     8701 ctccaatata tttgtatata tttccctcac agctatattt attctaattt aatattatga
     8761 ctttttaagg taattttttg tgacctgttc ggagtgatta gcgttacaat ttgaactgaa
     8821 agtgacatcc agtgtttgtt ccttgtgtag atgcatctca aaaaaatggt gggcataata
     8881 gtgttgttta tatatatcaa aaataacaac tataataata agaatacatt taatttagaa
     8941 aatgcttgga tttcactgga actagggcgc gcctccggaa cataatggtg cagggcgctg
     9001 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     9061 aggtcgcaga cgttttgcag cag
//
