LOCUS       sequence-243695-       11342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GAL80 fused with destabilizing domain (DD) downstream of a
            n-Synaptobrevin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11342)
  AUTHORS   Sethi S, Wang JW
  TITLE     A versatile genetic tool for post-translational control of gene 
            expression in Drosophila melanogaster.
  JOURNAL   Elife. 2017 Nov 15;6. pii: e30327. doi: 10.7554/eLife.30327.
  PUBMED    29140243
REFERENCE   2  (bases 1 to 11342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-11-15"; volume: "6. pii: e30327. doi"; pages: " 
            10.7554/eLife.30327"
COMMENT     Sequence Label: nSYB-GAL80-DD
FEATURES             Location/Qualifiers
     source          1..11342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..34
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1962..3266
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL80"
                     /product="transcriptional repressor that inhibits GAL4"
                     /label=GAL80
                     /note="GAL80 binds to and inhibits the transcriptional 
                     activation domain of GAL4."
                     /translation="MDYNKRSSVSTVPNAAPIRVGFVGLNAAKGWAIKTHYPAILQLSS
                     QFQITALYSPKIETSIATIQRLKLSNATAFPTLESFASSSTIDMIVIAIQVASHYEVVM
                     PLLEFSKNNPNLKYLFVEWALACSLDQAESIYKAAAERGVQTIISLQGRKSPYILRAKE
                     LISQGYIGDINSIEIAGNGGWYGYERPVKSPKYIYEIGNGVDLVTTTFGHTIDILQYMT
                     SSYFSRINAMVFNNIPEQELIDERGNRLGQRVPKTVPDHLLFQGTLLNGNVPVSCSFKG
                     GKPTKKFTKNLVIDIHGTKGDLKLEGDAGFAEISNLVLYYSGTRANDFPLANGQQAPLD
                     PGYDAGKEIMEVYHLRNYNAIVGNIHRLYQSISDFHFNTKKIPELPSQFVMQGFDFEGF
                     PTLMDALILHRLIESVYKSNMMGSTLNVSNISHYSL"
     protein_bind    4075..4144
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(4363..4381)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4402..4418)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4402..4418)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4415..4437)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4426..4442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4450..4456,4457..4474,4475..4480))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4495..4516
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4633..4650)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4804..5392)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4884..4903)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5563..6354,6355..6423))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6186..6205
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6424..6528)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6554..7009)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6691..6712)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6903..6922
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7136..7158
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7150..7167
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7151..7167
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7174..7193
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7174..7192
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7226..7244)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7696..7767,8169..8442,8517..9171,9233..9548,9752..9883,
                     9954..10568)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 ataacttcgt ataatgtatg ctatacgaag ttatgctagc ggatccgacc tcaaagatgg
       61 aagcggagca gaaggagcga tttcagacag acgcccccaa gttttgggat tccttctacg
      121 gcattcacga caaccgcttc ttcaaggatc gccactggct gttcacggag ttccccgaac
      181 tagctcctct ggcggcggat tcggcggtac tgcaaccacg cagcattttc gaactgggct
      241 gtggagtagg aaacacaatt ttaccccttc tacagtacag ctctgaaccg cagctcaagg
      301 ttttcggatg tgacttctcc gcccgggcca ttgaaatcct gcgaagccaa cggcagttcg
      361 acgagaagcg ctgcgaggtg tttgtcatgg atgccacgct ggatcattgg caggtgccct
      421 tcgaggaaaa ctcacaggac attattgtga tgatttttgt gctgtccgca attgagccaa
      481 agaaaatgca gcgggtattg gacaattgct atcgctacct gcgtcccggt ggcttgctcc
      541 tcttccgcga ctacggacga tacgacctgg cacagctgcg cttcaagagc ggcaagtgta
      601 tggaggacaa cttctacgtg cgaggcgacg gcaccatggt gtacttcttt acagaggaag
      661 aactgcgggg tatgatgact caagcggggc tgcaggagga gcagctcatt gtggaccgaa
      721 gactgcaagt taatcgctgt cgcggcttga agatgtaccg cgtgtggatt cagacaaagt
      781 tcaggaaacc gctttaaatc aatttagacg aatagttctg tgcattttaa accttgttca
      841 aatcaattga tataattatt tacacgatat atttaaaaat acaaagttaa aacttcaaac
      901 tgtaatatta ctagaacttt gagcatttct ttttcttgat ggaaagcccg gcttgttttc
      961 aaatttccca ccccttggcc atcggcactt ggaaatcgct aaggaaaagc ttctcgctgg
     1021 aacaccattc gctgctggcc atccaggcag ctttttcccc aaatacacat aacagttagg
     1081 aaagctgcaa gttttcgcag ttggccaaaa acgtcgtaag tgaaaatttt cctggtgctg
     1141 cgtaaaaaaa ttcgtaaaat attagttaag cctggttttt gtaaactcaa gttgcccgag
     1201 caaaagtaaa gtgattctga tcaaaagtgc taaaactgat tgattaggaa aacgaagatc
     1261 aaacgaaatg tcaaccaaag tcagcgaaaa cccttgtcgt gcagaatttc attgattttt
     1321 tcttctgagc ccacgttggc cagttgggca cttgaaatta tttttaaaac gttttgcatt
     1381 ttgaagacat cttaggtgcc cctgggggta tgcaacggaa atgaggaagg acatgcctca
     1441 tggctgggcg tgttgctgcc acccaaatag tcctcttacg tcccaattag aatcccatag
     1501 ctgcccattt caagcattaa ctgatgtact aaaaatagtt gcaactattt tcgcactcta
     1561 ttcagcagca gttccagcgc tgagccagaa aattcctgcg aaaggctgtc gtggcaagtg
     1621 ttacccgcac acatacacat catccagatc cagccaatcc ccccgtggag tggagtgaat
     1681 tttcattgac gacggggcga aaaagctctg gcggaagagc gacagagaga gagagaaaca
     1741 gagagagaga gagagaagcg gcactgagag cgcaaccctc tagaacaaaa acaacgaaac
     1801 agagttcagc tgcaccacca cccactacct acaccaccaa cgccaccacc gcccactgac
     1861 catcccacat cccgacctgc gccagtggaa gacagtgaaa gaggccaagt gcatcgtgca
     1921 tagagtacct tcatcctaat catcgacagc cgaattccaa catggactac aacaagagat
     1981 cttcggtctc aaccgtgcct aatgcagctc ccataagagt cggattcgtc ggtctcaacg
     2041 cagccaaagg atgggcaatc aagacacatt accccgccat actgcaacta tcgtcacaat
     2101 ttcaaatcac tgccttatac agtccaaaaa ttgagacttc tattgccacc attcagcgtc
     2161 taaaattgag taatgccact gcttttccca ctttagagtc atttgcatca tcttccacta
     2221 tagatatgat agtgatagct atccaagtgg ccagccatta tgaagttgtt atgcctctct
     2281 tggagttctc caaaaataat ccgaacctca agtatctttt cgtagaatgg gcccttgcat
     2341 gttcactaga tcaagccgaa tccatttata aggctgctgc tgaacgtggg gttcaaacca
     2401 tcatctcttt acaaggtcgt aaatcaccat atattttgag agcaaaagaa ttaatatctc
     2461 aaggctatat cggcgacatt aattcgatcg agattgctgg aaatggcggt tggtacggct
     2521 acgaaaggcc tgttaaatca ccaaaataca tctatgaaat cgggaacggt gtagatctgg
     2581 taaccacaac atttggtcac acaatcgata ttttacaata catgacaagt tcgtactttt
     2641 ccaggataaa tgcaatggtt ttcaataata ttccagagca agagctgata gatgagcgtg
     2701 gtaaccgatt gggccagcga gtcccaaaga cagtaccgga tcatctttta ttccaaggca
     2761 cattgttaaa tggcaatgtt ccagtgtcat gcagtttcaa aggtggcaaa cctaccaaaa
     2821 aatttaccaa aaatttggtc attgacattc acggtaccaa gggagatttg aaacttgaag
     2881 gcgatgccgg cttcgcagaa atttcaaatc tggtccttta ctacagtgga actagagcaa
     2941 acgacttccc gctagccaat ggacaacaag ctcctttaga cccggggtat gatgcaggta
     3001 aagaaatcat ggaagtatat catttacgaa attataatgc cattgtgggt aatattcatc
     3061 gactgtatca atctatctct gacttccact tcaatacaaa gaaaattcct gaattaccct
     3121 cacaatttgt aatgcaaggt ttcgatttcg aaggctttcc caccttgatg gatgctctga
     3181 tattacacag gttaatcgag agcgtttata aaagtaacat gatgggctcc acattaaacg
     3241 ttagcaatat ctcgcattat agtttactcg agatctccct gattgccgcc ctggccgtgg
     3301 accacgtgat tggcatggaa aatgccatgc cctggaacct gcccgccgat ctggcctggt
     3361 tcaagcgcaa caccttggac aagcccgtga tcatgggccg ccacacttgg gaaagcatcg
     3421 gtcgtccctt gcccggccgc aaggatatca ttctgagcag ccagccgtcc accgacgatc
     3481 gcgtcacttg ggtgaagagc gtcgacgagg ccatcgctgc ctgcggagat gtcccggaga
     3541 tcatggtgat cggaggagga cgtgtctacg agcagttcct gcccaaggcc cagaagctct
     3601 acctgaccca tatcgatgcc gaggtggagg gagataccca cttccccgac tacgagcccg
     3661 acggttggga gtcggtgttc tcggagttcc acgacgccga cgcccagaac tcgcactcgt
     3721 actgcttcga gattctggag cgccgttaat agacgtctat cgataccgtc gactaaagcc
     3781 aaatagaaaa ttattcagtt cctggcttaa gtttttaaaa gtgatattat ttatttggtt
     3841 gtaaccaacc aaaagaatgt aaataactaa tacataatta tgttagtttt aagttagcaa
     3901 caaattgatt ttagctatat tagctacttg gttaataaat agaatatatt tatttaaaga
     3961 taattgcgtt tttattgtca gggagtgagt ttgcttaaaa actcgtttag atccgcggcc
     4021 gcggctcgag ggtacctcta gagatccact agtgtcgacg atgtaggtca cggtctcgaa
     4081 gccgcggtgc gggtgccagg gcgtgccctt gggctccccg ggcgcgtact ccacctcacc
     4141 catctggtcc atcatgatga acgggtcgag gtggcggtag ttgatcccgg cgaacgcgcg
     4201 gcgcaccggg aagccctcgc cctcgaaacc gctgggcgcg gtggtcacgg tgagcacggg
     4261 acgtgcgacg gcgtcggcgg gtgcggatac gcggggcagc gtcagcgggt tctcgacggt
     4321 cacggcgggc atgtcgacac tagttctagc cagcttttgt tccctttagt gagggttaat
     4381 ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4441 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4501 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4561 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4621 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4681 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4741 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4801 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4861 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4921 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4981 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5041 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5101 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5161 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5221 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5281 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5341 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5401 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5461 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5521 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5581 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5641 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5701 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5761 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5821 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5881 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5941 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6001 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6061 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6121 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6181 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6241 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6301 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6361 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6421 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6481 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6541 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     6601 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     6661 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     6721 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     6781 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     6841 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     6901 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     6961 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
     7021 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     7081 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     7141 tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg
     7201 ggtacgtacc gggcccctag tatgtatgta agttaataaa acccattttt gcggaaagta
     7261 gataaaaaaa acattttttt tttttactgc actggatatc attgaactta tctgatcagt
     7321 tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat tacctctcac
     7381 tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc cacagaaata
     7441 tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt agcgttacgt
     7501 agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa gctccaagcg
     7561 gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct tcttattttt
     7621 gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca ggggaaagtg
     7681 tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg aggcagcaaa
     7741 cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat cttaagttca
     7801 cttgatctat aggaactgcg attgcaacat caaattgtct gcggcgtgag aactgcgacc
     7861 cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag attattctgg
     7921 tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag acatctaaag
     7981 gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat attttaagct
     8041 gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga tacttcgggg
     8101 tactttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga gatcccctat
     8161 cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc ttcgggcagg
     8221 ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt tcagggagcg
     8281 gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt ggggcggtca
     8341 atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta ttctgcaacg
     8401 agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt ctattcgcag
     8461 tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa actcagtttg
     8521 cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg ccggaaagac
     8581 gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat cgccatccgg
     8641 gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca ggtgcgccta
     8701 tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc tgattttcca
     8761 agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc gcgtggatca
     8821 ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg tgcccggcag
     8881 ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg aggctctaac
     8941 cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct ttaccgccca
     9001 cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca tcctgaccat
     9061 tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga tggccgaggg
     9121 cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct agtgagttcg
     9181 atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc agcgtgggtg
     9241 cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg gccgttgtgc
     9301 ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat tttgccatta
     9361 gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag aagccactgg
     9421 agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc cgggcggtcc
     9481 tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg cgacttattc
     9541 agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat tcttggaaac
     9601 aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt ttttttcaat
     9661 gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact cgcgaatatt
     9721 aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg gcctcatctt
     9781 tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca tcttcctctt
     9841 cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc atgtttagaa
     9901 tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt taggtgttca
     9961 cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc tgtgacacat
    10021 actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca ctggtcttca
    10081 cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc ttcaactgcc
    10141 tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta atatcctgcg
    10201 ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata ccattcctgc
    10261 tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa tggttgtcgt
    10321 acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg gcggacgtgg
    10381 agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg ggcaaggtca
    10441 tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg ggtctggcca
    10501 ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg gcccgacgca
    10561 aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc attattacca
    10621 tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg tgccaataaa
    10681 aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca caagtagact
    10741 ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc aaaaactcgt
    10801 ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca aatcgcgcgc
    10861 cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg ctgtctccca
    10921 cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact gtcgttagtt
    10981 ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca atagaaatta
    11041 gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat atttccctca
    11101 cagctatatt tattctaatt taatattatg actttttaag gtaatttttt gtgacctgtt
    11161 cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt tccttgtgta
    11221 gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca aaaataacaa
    11281 ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg aactaggcta
    11341 gc
//
