LOCUS       sequence-243701-        8709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human MTP in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Sellers JA, Hou L, Athar H, Hussain MM, Shelness GS
  TITLE     A Drosophila microsomal triglyceride transfer protein homolog 
            promotes the assembly and secretion of human apolipoprotein B. 
            Implications for human and insect transport and metabolism.
  JOURNAL   J Biol Chem. 2003 May 30;278(22):20367-73. doi: 
            10.1074/jbc.M300271200. Epub 2003 Mar 25.
  PUBMED    12657646
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M300271200"; journalName: "J Biol Chem"; date: 
            "2003-05-30- 30"; volume: "278"; issue: "22"; pages: "20367-73"
COMMENT     Sequence Label: hMTP
FEATURES             Location/Qualifiers
     source          1..8709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        79..458
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        459..662
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     613..633
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     707..726
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        707..725
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4012..4030)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4013..4030)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4050..4067)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4056..4280
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4326..4839
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4413..4432)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4623..4644
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4862..4882)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4867..5196
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5047..5182
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5263..6057
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5317..5336)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5927..5946
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6231..6365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6268..6287)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6322..6341
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6396..6412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6396..6412)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6409..6431)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6420..6436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6444..6450,6451..6468,6469..6474))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6489..6510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6627..6644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6798..7386)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6878..6897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7557..8348,8349..8417))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8180..8199
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8418..8522)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8597..8616)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
       61 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      121 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      181 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      241 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      301 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      361 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      421 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      481 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      541 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      601 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      661 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
      721 atagggagac ccaagcttgg taccgagctc ggatcccccg ggctgcagga attcggcacg
      781 agtgaaagag tccacttctc agtgactcct agctgggcac tggatgcagt tgaggattgc
      841 tggtcaatat gattcttctt gctgtgcttt ttctctgctt catttcctca tattcagctt
      901 ctgttaaagg tcacacaact ggtctctcat taaataatga ccggctgtac aagctcacgt
      961 actccactga agttcttctt gatcggggca aaggaaaact gcaagacagc gtgggctacc
     1021 gcatttcctc caacgtggat gtggccttac tatggaggaa tcctgatggt gatgatgacc
     1081 agttgatcca aataacgatg aaggatgtaa atgttgaaaa tgtgaatcag cagagaggag
     1141 agaagagcat cttcaaagga aaaagcccat ctaaaataat gggaaaggaa aacttggaag
     1201 ctctgcaaag acctacgctc cttcatctaa tccatggaaa ggtcaaagag ttctactcat
     1261 atcaaaatga ggcagtggcc atagaaaata tcaagagagg tctggctagc ctatttcaga
     1321 cacagttaag ctctggaacc accaatgagg tagatatctc tggaaattgt aaagtgacct
     1381 accaggctca tcaagacaaa gtgatcaaaa ttaaggcctt ggattcatgc aaaatagcga
     1441 ggtctggatt tacgacccca aatcaggtct tgggtgtcag ttcaaaagct acatctgtca
     1501 ccacctataa gatagaagac agctttgtta tagctgtgct tgctgaagaa acacacaatt
     1561 ttggactgaa tttcctacaa accattaagg ggaaaatagt atcgaagcag aaattagagc
     1621 tgaagacaac cgaagcaggc ccaagattga tgtctggaaa gcaggctgca gccataatca
     1681 aagcagttga ttcaaagtac acggccattc ccattgtggg gcaggtcttc cagagccact
     1741 gtaaaggatg tccttctctc tcggagctct ggcggtccac caggaaatac ctgcagcctg
     1801 acaacctttc caaggctgag gctgtcagaa acttcctggc cttcattcag cacctcagga
     1861 ctgcgaagaa agaagagatc cttcaaatac taaagatgga aaataaggaa gtattacctc
     1921 agctggtgga tgctgtcacc tctgctcaga cctcagactc attagaagcc attttggact
     1981 ttttggattt caaaagtgac agcagcatta tcctccagga gaggtttctc tatgcctgtg
     2041 gatttgcttc tcatcccaat gaagaactcc tgagagccct cattagtaag ttcaaaggtt
     2101 ctattggtag cagtgacatc agagaaactg ttatgatcat cactgggaca cttgtcagaa
     2161 agttgtgtca gaatgaaggc tgcaaactca aagcagtagt ggaagctaag aagttaatcc
     2221 tgggaggact tgaaaaagca gagaaaaaag aggacaccag gatgtatctg ctggctttga
     2281 agaatgccct gcttccagaa ggcatcccaa gtcttctgaa gtatgcagaa gcaggagaag
     2341 ggcccatcag ccacctggct accactgctc tccagagata tgatctccct ttcataactg
     2401 atgaggtgaa gaagacctta aacagaatat accaccaaaa ccgtaaagtt catgaaaaga
     2461 ctgtgcgcac tgctgcagct gctatcattt taaataacaa tccatcctac atggacgtca
     2521 agaacatcct gctgtctatt ggggagcttc cccaagaaat gaataaatac atgctcgcca
     2581 ttgttcaaga catcctacgt tttgaaatgc ctgcaagcaa aattgtccgt cgagttctga
     2641 aggaaatggt cgctcacaat tatgaccgtt tctccaggag tggatcttct tctgcctaca
     2701 ctggctacat agaacgtagt ccccgttcgg catctactta cagcctagac attctctact
     2761 cgggttctgg cattctaagg agaagtaacc tgaacatctt tcagtacatt gggaaggctg
     2821 gtcttcacgg tagccaggtg gttattgaag cccaaggact ggaagcctta atcgcagcca
     2881 cccctgacga gggggaggag aaccttgact cctatgctgg tatgtcagcc atcctctttg
     2941 atgttcagct cagacctgtc acctttttca acggatacag tgatttgatg tccaaaatgc
     3001 tgtcagcatc tggcgaccct atcagtgtgg tgaaaggact tattctgcta atagatcatt
     3061 ctcaggaact tcagttacaa tctggactaa aagccaatat agaggtccag ggtggtctag
     3121 ctattgatat ttcaggtgca atggagttta gcttgtggta tcgtgagtct aaaacccgag
     3181 tgaaaaatag ggtgactgtg gtaataacca ctgacatcac agtggactcc tcttttgtga
     3241 aagctggcct ggaaaccagt acagaaacag aagcaggctt ggagtttatc tccacagtgc
     3301 agttttctca gtacccattc ttagtttgca tgcagatgga caaggatgaa gctccattca
     3361 ggcaatttga gaaaaagtac gaaaggctgt ccacaggcag aggttatgtc tctcagaaaa
     3421 gaaaagaaag cgtattagca ggatgtgaat tcccgctcca tcaagagaac tcagagatgt
     3481 gcaaagtggt gtttgcccct cagccggata gtacttccag cggatggttt tgaaactgac
     3541 ctgtgatatt ttacttgaat ttgtctcccc gaaagggaca caatgtggca tgactaagta
     3601 cttgctctct gagagcacag cgtttacata tttacctgta tttaagattt ttgtaaaaag
     3661 ctacaaaaaa ctgcagtttg atcaaatttg ggtatatgca gtatgctacc cacagcgtca
     3721 ttttgaatca tcatgtgacg ctttcaacaa cgttcttagt ttacttatac ctctctcaaa
     3781 tctcatttgg tacagtcaga atagttattc tctaagagga aactagtgtt tgttaaaaac
     3841 aaaaataaaa acaaaaccac acaaggagaa cccaattttg tttcaacaat ttttgatcaa
     3901 tgtatatgaa gctcttgata ggacttcctt aagcatgacg ggaaaaccaa acacgttccc
     3961 taatcaggaa aaaaaaaaaa aaaactcgag catgcatcta gagggcccta ttctatagtg
     4021 tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     4081 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4141 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     4201 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     4261 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct cgagggggga
     4321 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4381 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4441 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4501 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4561 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4621 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4681 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4741 atttaacgcg aattttaaca aaatattaac gcttacaatt taaatatttg cttatacaat
     4801 cttcctgttt ttggggcttt tctgattatc aaccggggtg ggtaccgagc tcgaattctg
     4861 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4921 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     4981 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5041 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5101 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     5161 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttggatatcc
     5221 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     5281 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     5341 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     5401 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     5461 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     5521 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     5581 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     5641 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     5701 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     5761 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     5821 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     5881 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     5941 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6001 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     6061 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     6121 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     6181 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     6241 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6301 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6361 ggatccgtcg acctcgagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6421 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6481 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6541 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6601 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6661 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6721 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6781 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6841 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6901 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6961 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7021 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7081 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7141 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7201 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7261 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7321 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7381 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7441 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7501 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7561 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7621 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7681 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7741 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7801 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7861 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7921 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7981 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8041 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8101 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8161 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8221 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8281 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8341 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8401 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8461 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8521 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     8581 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     8641 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     8701 aaggcttga
//
