LOCUS       sequence-243703-       10061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Hussain NK, Jenna S, Glogauer M, Quinn CC, Wasiak S, Guipponi M, 
            Antonarakis SE, Kay BK, Stossel TP, Lamarche-Vane N, McPherson PS
  TITLE     Endocytic protein intersectin-l regulates actin assembly via Cdc42 
            and N-WASP.
  JOURNAL   Nat Cell Biol. 2001 Oct;3(10):927-32.
  PUBMED    11584276
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "2001-10"; volume: "3(10)"; pages: "927-32"
COMMENT     Sequence Label: GFP-Intersectin Long
FEATURES             Location/Qualifiers
     source          1..10061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        127..430
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        431..634
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     585..605
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      673..682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(679..681,682..684,685..1395)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(679..681,682..684,685..1395)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(724..745)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(985..1004)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1332..1353
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6915..7036
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6952..6971)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7006..7025
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7043..7498)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7180..7201)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7392..7411
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7525..7629
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7631..7988
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7631..7822
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7654..7674)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7839..7974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8023..8817
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8023..8817
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8077..8096)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8687..8706
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9005..9024)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9049..9096
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9425..10013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9914..9933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
       61 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      121 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      181 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      241 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      301 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      361 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      421 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      481 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      541 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      601 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      661 gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      721 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      781 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      841 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
      901 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      961 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1021 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1081 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1141 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1201 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1261 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     1321 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1381 gacgagctgt acaagtccgg ccggactcag atctcgaggg ctcagtttcc aacacctttt
     1441 ggtggcagcc tggatatctg ggccataact gtagaggaaa gagcgaagca tgatcagcag
     1501 ttccatagtt taaagccaat atctggattc attactggtg atcaagctag aaactttttt
     1561 tttcaatctg ggttacctca acctgtttta gcacagatat gggcactagc tgacatgaat
     1621 aatgatggaa gaatggatca agtggagttt tccatagcta tgaaacttat caaactgaag
     1681 ctacaaggat atcagctacc ctctgcactt ccccctgtca tgaaacagca accagttgct
     1741 atttctagcg caccagcatt tggtatggga ggtatcgcca gcatgccacc gcttacagct
     1801 gttgctccag tgccaatggg atccattcca gttgttggaa tgtctccaac cctagtatct
     1861 tctgttccca cagcagctgt gccccccctg gctaacgggg ctccccctgt tatacaacct
     1921 ctgcctgcat ttgctcatcc tgcagccaca ttgccaaaga gttcttcctt tagtagatct
     1981 ggtccagggt cacaactaaa cactaaatta caaaaggcac agtcatttga tgtggccagt
     2041 gtcccaccag tggcagagtg ggctgttcct cagtcatcaa gactgaaata caggcaatta
     2101 ttcaatagtc atgacaaaac tatgagtgga cacttaacag gtccccaagc aagaactatt
     2161 cttatgcagt caagtttacc acaggctcag ctggcttcaa tatggaatct ttctgacatt
     2221 gatcaagatg gaaaacttac agcagaggaa tttatcctgg caatgcacct cattgatgta
     2281 gctatgtctg gccaaccact gccacctgtc ctgcctccag aatacattcc accttctttt
     2341 agaagagttc gatctggcag tggtatatct gtcataagct caacatctgt agatcagagg
     2401 ctaccagagg aaccagtttt agaagatgag caacaacaat tagaaaagaa attacctgta
     2461 acgtttgaag ataagaagcg ggagaacttt gaacgtggca acctggaact ggagaaacga
     2521 aggcaagctc tcctggaaca gcagcgcaag gagcaggagc gcctggccca gctggagcgg
     2581 gcggagcagg agaggaagga gcgtgagcgc caggagcaag agcgcaaaag acaactggaa
     2641 ctggagaagc aactggaaaa gcagcgggag ctagaacggc agagagagga ggagaggagg
     2701 aaagaaattg agaggcgaga ggctgcaaaa cgggaacttg aaaggcaacg acaacttgag
     2761 tgggaacgga atcgaaggca agaactacta aatcaaagaa acaaagaaca agaggacata
     2821 gttgtactga aagcaaagaa aaagactttg gaatttgaat tagaagctct aaatgataaa
     2881 aagcatcaac tagaagggaa acttcaagat atcagatgtc gattgaccac ccaaaggcaa
     2941 gaaattgaga gcacaaacaa atctagagag ttgagaattg ccgaaatcac ccatctacag
     3001 caacaattac aggaatctca gcaaatgctt ggaagactta ttccagaaaa acagatactc
     3061 aatgaccaat taaaacaagt tcagcagaac agtttgcaca gagattcact tgttacactt
     3121 aaaagagcct tagaagcaaa agaactagct cggcagcacc tacgagacca actggatgaa
     3181 gtggagaaag aaactagatc aaaactacag gagattgata ttttcaataa tcagctgaag
     3241 gaactaagag aaatacacaa taagcaacaa ctccagaagc aaaagtccat ggaggctgaa
     3301 cgactgaaac agaaagaaca agaacgaaag atcatagaat tagaaaaaca aaaagaagaa
     3361 gcccaaagac gagctcagga aagggacaag cagtggctgg agcatgtgca gcaggaggac
     3421 gagcatcaga gaccaagaaa actccacgaa gaggaaaaac tgaaaaggga ggagagtgtc
     3481 aaaaagaagg atggcgagga aaaaggcaaa caggaagcac aagacaagct gggtcggctt
     3541 ttccatcaac accaagaacc agctaagcca gctgtccagg caccctggtc cactgcagaa
     3601 aaaggtccac ttaccatttc tgcacaggaa aatgtaaaag tggtgtatta ccgggcactg
     3661 tacccctttg aatccagaag ccatgatgaa atcactatcc agccaggaga catagtcatg
     3721 gttaaagggg aatgggtgga tgaaagccaa actggagaac ccggctggct tggaggagaa
     3781 ttaaaaggaa agacagggtg gttccctgca aactatgcag agaaaatccc agaaaatgag
     3841 gttcccgctc cagtgaaacc agtgactgat tcaacatctg cccctgcccc caaactggcc
     3901 ttgcgtgaga cccccgcccc tttggcagta acctcttcag agccctccac gacccctaat
     3961 aactgggccg acttcagctc cacgtggccc accagcacga atgagaaacc agaaacggat
     4021 aactgggatg catgggcagc ccagccctct ctcaccgttc caagtgccgg ccagttaagg
     4081 cagaggtccg cctttactcc agccacggcc actggctcct ccccgtctcc tgtgctaggc
     4141 cagggtgaaa aggtggaggg gctacaagct caagccctat atccttggag agccaaaaaa
     4201 gacaaccact taaattttaa caaaaatgat gtcatcaccg tcctggaaca gcaagacatg
     4261 tggtggtttg gagaagttca aggtcagaag ggttggttcc ccaagtctta cgtgaaactc
     4321 atttcagggc ccataaggaa gtctacaagc atggattctg gttcttcaga gagtcctgct
     4381 agtctaaagc gagtagcctc tccagcagcc aagccggtcg tttcgggaga agaatttatt
     4441 gccatgtaca cttacgagag ttctgagcaa ggagatttaa cctttcagca aggggatgtg
     4501 attttggtta ccaagaaaga tggtgactgg tggacaggaa cagtgggcga caaggccgga
     4561 gtcttccctt ctaactatgt gaggcttaaa gattcagagg gctctggaac tgctgggaaa
     4621 acagggagtt taggaaaaaa acctgaaatt gcccaggtta ttgcctcata caccgccacc
     4681 ggccccgagc agctcactct cgcccctggt cagctgattt tgatccgaaa aaagaaccca
     4741 ggtggatggt gggaaggaga gctgcaagca cgtgggaaaa agcgccagat aggctggttc
     4801 ccagctaatt atgtaaagct tctaagccct gggacgagca aaatcactcc aacagagcca
     4861 cctaagtcaa cagcattagc ggcagtgtgc caggtgattg ggatgtacga ctacaccgcg
     4921 cagaatgacg atgagctggc cttcaacaag ggccagatca tcaacgtcct caacaaggag
     4981 gaccctgact ggtggaaagg agaagtcaat ggacaagtgg ggctcttccc atccaattat
     5041 gtgaagctga ccacagacat ggacccaagc cagcaatggt gttcagactt acatctcttg
     5101 gatatgttga ccccaactga aagaaagcga caaggataca tccacgagct cattgtcacc
     5161 gaggagaact atgtgaatga cctgcagctg gtcacagaga tttttcaaaa acccctgatg
     5221 gagtctgagc tgctgacaga aaaagaggtt gctatgattt ttgtgaactg gaaggagctg
     5281 attatgtgta atatcaaact actaaaagcg ctgagagtcc gcaagaagat gtccggggag
     5341 aagatgcctg tgaagatgat tggagacatc ctgagcgcac agctgccgca catgcagccc
     5401 tacatccgct tctgcagccg ccagctcaac ggggctgccc tgatccagca gaagacggac
     5461 gaggccccag acttcaagga gttcgtcaaa agattggcaa tggatcctcg gtgtaaaggg
     5521 atgccactct ctagttttat actgaagcct atgcaacggg taacaagata cccactgatc
     5581 attaaaaata tcctggaaaa cacccctgaa aaccacccgg accacagcca cttgaagcac
     5641 gccctggaga aggcggaaga gctctgttcc caggtgaacg aaggggtgcg ggagaaggag
     5701 aactctgacc ggctggagtg gatccaggcc cacgtgcagt gtgaaggcct gtctgagcaa
     5761 cttgtgttca attcagtgac caattgcttg gggccgcgca aatttctgca cagtgggaag
     5821 ctctacaagg ccaagagcaa caaggagctg tatggcttcc ttttcaacga cttcctcctg
     5881 ctgactcaga tcacgaagcc tttggggtct tctggcaccg acaaagtctt cagccccaaa
     5941 tcaaacctgc agtataaaat gtataaaaca cctattttcc taaatgaggt tctagtaaaa
     6001 ttacccaccg acccttctgg agacgagccc atcttccaca tctcccacat tgaccgcgtc
     6061 tatactctcc gagcagaaag cataaatgaa aggactgcct gggtgcagaa aatcaaagct
     6121 gcttctgaac tctacataga gactgagaaa aagaagcgcg agaaagcgta cctggtccgt
     6181 tcccaaaggg caacaggcat tggaaggttg atggtgaacg tggttgaagg catcgagttg
     6241 aaaccctgtc ggtcacatgg aaagagcaac ccgtactgtg aggtgaccat gggttcccag
     6301 tgccacatca ccaagacgat ccaggacact ctgaacccca agtggaattc caactgccag
     6361 ttcttcatcc gagacctgga gcaggaagtc ctctgcatca ctgtgttcga gagggaccag
     6421 ttctcaccag atgatttttt gggtcggacg gagatccgtg tggcggacat caagaaagac
     6481 cagggctcca aaggtccagt tacgaagtgt cttctgctgc acgaagtccc cacgggagag
     6541 attgtggtcc gcttggacct gcagttgttt gatgagccgt aggcagcggg ctcagggtgt
     6601 gctcagcagg gtcccagccc acggccacac atgctgtctg gaaattgtat tccttttcta
     6661 agaaaccacc atttggtatt cagtcacagg gatatgggat ggcaaagacc cggaattctt
     6721 ttgcttttta ccctggaaga aatactcata agcttatcga taccgtcgac ggtaccgcgg
     6781 gcccgggatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
     6841 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     6901 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6961 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7021 tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
     7081 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     7141 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     7201 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     7261 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     7321 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     7381 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     7441 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
     7501 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7561 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7621 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     7681 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7981 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     8041 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     8101 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     8161 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     8221 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     8281 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     8341 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     8401 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     8461 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     8521 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     8581 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     8641 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     8701 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     8761 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     8821 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     8881 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     8941 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc
     9001 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     9061 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     9121 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     9181 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     9241 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac
     9301 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9361 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9421 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9481 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9541 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     9601 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9661 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9721 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9781 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9841 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9901 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9961 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10021 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc t
//
