LOCUS       sequence-243709-       11421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing an mRuby-tagged TIR1 in the soma of C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11421)
  AUTHORS   Zhang L, Ward JD, Cheng Z, Dernburg AF
  TITLE     The auxin-inducible degradation (AID) system enables versatile 
            conditional protein depletion in C. elegans.
  JOURNAL   Development. 2015 Nov 9. pii: dev.129635.
  PUBMED    26552885
REFERENCE   2  (bases 1 to 11421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2015 Nov 9. pii: dev.129635."
COMMENT     Sequence Label: pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54...
FEATURES             Location/Qualifiers
     source          1..11421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1491..1502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3601..4200
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     primer_bind     9406..9428
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9466..9484)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9552..9656
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9657..9725,9726..10517)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9875..9894)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10688..11276
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11177..11196
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggccctgtc ggattatggg ctcttctttt atcctctact ttctttctag aaaatgcaaa
       61 tgttgagtca gaaattgctg aatggagtac aactgcaatg ggtttagctt atgtttctgg
      121 aacaattact ggtggaattg tgattgaaaa gtaaggttat atttcatttc aataaatgtt
      181 accacagtat aacaaaaaca agttacattg ttcagagtat gtctgaatgt attattatta
      241 ttgtatttgt gcatcagtac tgtagttgta ctgaagaagg gtttaaaata atctcaacgt
      301 ttttaagacg agttagtgag aatacggtcg cagttaggat gtttggacat agttttatta
      361 gataatcgtg aactaactca ctataaaaac ccgctaactc attgaataag atagttttaa
      421 acgttcaaaa tttgaagaca aaattgtatg aataagttta cagagttggt cgacgcaagc
      481 ttctccttct cttcacattt ctcaataatt tagctctaat tgcattcgta ttcttcgcaa
      541 aaatacgtat tctgattgac ccgatgaaat acggatgcct agttgcattg atcatctacg
      601 gttatactta tgggtgaggt ttttcaaata tattttgatt taatcgaata atgaaatttc
      661 agaactggag ttggtccaat ttcgtggttt atatcctctg aactggtacc tcaaaaacat
      721 cgtagtattg ctcaatctgt ggcatattca atgaatactg taatggtggt tatcagtaca
      781 tttacagtat tacctctata ttccgttatt ggtagttatg ctttccttat tctatattca
      841 attccttcat ttataagtat gcttatatta tttcgctact taccggaaac caaaggacga
      901 gagattcatg atattgtgaa tgaacttaaa aaaaaatgaa aacctttgaa aacgttcata
      961 cggccgaaat tttacttgat cgagccaatt gaacttttgt taagacttac agaaaagtga
     1021 gagattttgt tacaaaaaag tgagaaaaaa gcgaaaatga acaggaaaaa gaagaaaaat
     1081 acacccgaag tatcatataa aaattaaaaa gattgtttga cctggcggaa cttttttgca
     1141 aaaactctga atatccatgg cacatgcttc gtgcaaaaca ggatccaaat gaatatcaac
     1201 aagagattct tgaagacgac gagccacttg ctcagagcat tgcttatctc gaatgagacc
     1261 cttgttaaaa tcatttttca aacattatat gacattgtcg aaatgtcctc ctgattccat
     1321 gatggtagca aactcacttc gtctttgagc caatttatcc aagtccttgt aaaaagtatt
     1381 cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc
     1441 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct
     1501 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc
     1561 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct
     1621 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat
     1681 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat
     1741 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct
     1801 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg
     1861 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc
     1921 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca
     1981 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca ataggtttct
     2041 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt
     2101 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc
     2161 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa
     2221 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct
     2281 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg
     2341 agcaacaacg atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa
     2401 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt
     2461 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat
     2521 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact
     2581 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag
     2641 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc
     2701 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga
     2761 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt
     2821 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc
     2881 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg
     2941 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc
     3001 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct
     3061 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt
     3121 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat
     3181 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga
     3241 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg
     3301 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact
     3361 cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat
     3421 tttcaatttc aactgaaaga ttttttcatt agagaatgtc tagaactagg cccgggctac
     3481 gtaatacgac tcacttaagg ccttgactag agggtaccag agctcaccta ggtatctaga
     3541 accggtgacg tcaccatggg aagcttcgtg gatccagata tcctgcagga attcctcgag
     3601 gcacctttgg tcttttattg tcaacttcca ttggttcttc cattgtttct gttaaattaa
     3661 tgaatttttc ataaaataaa gacattatac aatataaaaa tgaagaattt attgaaaata
     3721 aactgccaga gagaaaaagt atgcaacact cccgccgaga gtgtttgaaa tggtgtacgg
     3781 tacattttcg tgctaggagt tagatgtgca ggcagcaacg agagggggag agattttttt
     3841 gggccttgtg aaattaacgt gagttttctg gtcatctgac taatcatgtt ggttttttgt
     3901 tggtttattt tgtttttatc tttgttttta tccagattag gaaatttaaa ttttatgaat
     3961 ttataatgag gtcaaacatt cagtcccagc gtttttcctg ttctcactgt ttagtcgaat
     4021 ttttatttta ggctttcaac aaatgttcta actgtcttat ttgtgacctc actttttata
     4081 tttttttaat ttttaaaaat attagaagtt tctaggataa ttttttcgac ttttattctc
     4141 tctaccgtcc gcactcttct tacttttaaa ttaaattgtt tttttttcag ttgggaaaca
     4201 ctttgctcat gcaaaagaga atcgccttgt cgtttccaga agaagtactc gagcacgtct
     4261 tctccttcat ccaactcgac aaggaccgta actccgtctc cctcgtctgc aagtcctggt
     4321 acgagatcga gcgttggtgc cgtcgtaagg tcttcatcgg aaactgctac gccgtctccc
     4381 cagccaccgt catccgtcgt ttcccaaagg tccgttccgt cgagctcaag ggaaagccac
     4441 acttcgccga cttcaacctc gtcccagacg gatggggagg atacgtctac ccatggatcg
     4501 aggccatgtc ctcctcctac acctggctcg aggagatccg tctcaagcgt atggtcgtca
     4561 ccgacgactg cctcgagctc atcgccaagt ccttcaagaa cttcaaggtc ctcgtcctct
     4621 cctcctgcga gggattctcc accgacggac tcgccgccat cgccgccacc tgccgtaacc
     4681 tcaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcaggagc
     4741 tcgacctccg tgagtccgac gtcgacgagg tctccggaca ctggctctcc cacttcccag
     4801 acacctacac ctccctcgtc tccctcaaca tctcctgcct cgcctccgag gtctccttct
     4861 ccgccctcga gcgtctcgtc acccgttgcc caaacctcaa gtccctcaag ctcaaccgtg
     4921 ccgtcccact cgagaagctc gccaccctcc tccaacgtgc cccacaactc gaggagctcg
     4981 gaaccggagg atacaccgcc gaggtccgtc cagacgtcta ctccggactc tccgtcgccc
     5041 tctccggatg caaggtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
     5101 ttcaggagct ccgttgcctc tccggattct gggacgccgt cccagcctac ctcccagccg
     5161 tctactccgt ctgctcccgt ctcaccaccc tcaacctctc ctacgccacc gtccaatcct
     5221 acgacctcgt caagctcctc tgccaatgcc caaagctcca acgtctctgg gtcctcgact
     5281 acatcgagga cgccggactc gaggtcctcg cctccacctg caaggacctc cgtgagctcc
     5341 gtgtcttccc atccgagcca ttcgtcatgg agccaaacgt cgccctcacc gagcaaggac
     5401 tcgtctccgt ctccatggga tgcccaaagc tcgagtccgt cctctacttc tgccgtcaaa
     5461 tgaccaacgc cgccctcatc accatcgccc gtaaccgtcc aaacatgacc cgtttccgtc
     5521 tctgcatcat cgagccaaag gccccagact acctcaccct cgagccactc gacatcggat
     5581 tcggagccat cgtcgagcac tgcaaggacc tccgtcgtct ctccctctcc ggactcctca
     5641 ccgacaaggt cttcgagtac atcggaacct acgccaagaa gatggagatg ctctccgtcg
     5701 ccttcgccgg agactccgac ctcggactcc accacgtcct ctccggatgc gactccctcc
     5761 gtaagctcga gatccgtgac tgcccattcg gagacaaggc cctcctcgcc aacgcctcca
     5821 agctcgagac catgcgttcc ctctggatgt cctcctgctc cgtctccttc ggagcctgca
     5881 agctcctcgg acaaaagatg ccaaagctca acgtcgaggt catcgacgag cgtggagccc
     5941 cagactcccg tccagagtcc tgcccagtcg agcgtgtctt catctaccgt accgtcgccg
     6001 gaccacgttt cgacatgcca ggattcgtct ggaacatgga ccaagactcc accatgcgtt
     6061 tctcccgtca aatcatcacc accaacggac tcggaggatc tggaggcggt tctggcggag
     6121 gttctggtat gaactctctt atcaaggaaa acatgcgcat gaaggttgtt cttgagggat
     6181 ctgttaacgg acatcagttc aagtgcactg gagaaggaga aggaaatcca tacatggtat
     6241 gtgatacatg gaaataggca cattcattaa atatttgtat tttcagggaa ctcaaactat
     6301 gcgcatcaag gttatcgaag gaggaccact tccattcgct ttcgacatcc ttgctacctc
     6361 tttcatgtac ggatctcgca ctttcattaa gtatccaaag ggaattccag atttcttcaa
     6421 gcagtctttc ccagaaggat tcacttggga gcgcgttacc cgctacgagg atggaggagt
     6481 tatcaccgtt atgcaagata cctcccttga ggatggatgc cttgtttacc acgctcaagt
     6541 tcgtggagtt aacttcccat ctaacggagc tgttatgcag aagaagacta agggatggga
     6601 accaaacact gaaatgatgt acccagctga tggaggactt cgcggataca cccacatggc
     6661 tcttaaggtt gatggaggag gacacctttc ttgctctttc gttaccacct accgctctaa
     6721 gaagactgtt ggaaatatta agatgccagg aatccatgct gtcgatcacc gtcttgagcg
     6781 tcttgaggag tctgataacg agatgttcgt tgttcaacgt gagcacgctg tcgctaagtt
     6841 cgctggactt ggaggaggat aagagctccg catcggccgc tgtcatcaga tcgccatctc
     6901 gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct
     6961 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt
     7021 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
     7081 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
     7141 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt
     7201 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     7261 atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca
     7321 tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt
     7381 aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg
     7441 cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag
     7501 gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt
     7561 gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt
     7621 ttactagtga gtcgtattat aagtgcaagt aagatcagtg tttgtttcga tatcagtctg
     7681 tttcgtaacg gtcttctgta taactacaaa aaagaataaa aaaccgtatc acaactgaga
     7741 aaaaagacaa aacttattga acagtgttcc tttcaaaaaa ttattttgta aaaaatcgag
     7801 tttttctcca aaataacgca cgcaatcaaa aacaagaaaa ctaacgtgtt ctcctgggcg
     7861 tggaactcct tatcaagatt accattattg tctgtcagaa ttgatatttc aattatttca
     7921 tatctagaca aacccgggtg tcttgcattt attagtactg tgagtttaca tacaaacgag
     7981 gcttactcag ataacataga gtcacctcta attagcaaaa acccatttct aattagctat
     8041 ttcgaccagt tctaaagtca ccgtaattag aaatggtttt tgtagctttt ctaattagaa
     8101 atgaccccga gataagtgaa atatggcatt ttctgggttt tctagtaaga aatgatcccg
     8161 cacgtagtga ttccgcgtta caagccaaat tcaaattaaa aaagtatatt acacaattca
     8221 agttacatta ttttcaggaa acaatggcga agagcaaagc agagattcaa aaggaggaaa
     8281 gagacaacag atatgaacga agacttcttg aaaagagaaa gagacatgag gtgagtaacc
     8341 atgataataa ataatcaagt aagggctata gatttataaa ttctggagtt aacagtttcg
     8401 aaaaaaagta gttttcatat gtgcgaagga atatcaaaaa taggggaata gcctaaacga
     8461 gtgagaaaat taaatgtttg ttattttttc aaaaatgtct gcagggccgt tatcctttca
     8521 aatgcactct atcaaacata gatcatatcc atcatatctt atccagaatt tatatcatat
     8581 ccaagattga gagcgtttca aggaaaatct caaacaacca tacaattttg tagatcgaca
     8641 acaaggcctc cacttcttca tcgatggcct ctgcagcacc tgcaccacca agtttgtatt
     8701 tttcatatta atataaagtg agccacgcat agtcgttttt aggatttttg ccagatcgat
     8761 gtcgcgcaaa cagactgttt ttttttcttt gaaagccgtg cgtgacatgg gccacggatt
     8821 gtaacgccgc gctggatgga taaaaattta ttcagttttt tagcctttac aaaatatggt
     8881 tcgactactt tctttatttt tacattttta taggtgctca ttgagctgag atattttggg
     8941 tataatcgga aaatcgctga ttttggtcat ttttctgcac taaagattac acaaaaagtt
     9001 cagtatttag attcattgtt gaaaattggg gatgatctac agatacatat cgccgtgttc
     9061 aaaaacacac gtgctgtgac gtcgtgactg gggaaaaccc tggcgttacc caacttaatc
     9121 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     9181 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     9241 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtgca atctgctctg
     9301 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     9361 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     9421 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     9481 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     9541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9601 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     9661 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     9721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     9781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     9841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     9901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     9961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    10021 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    10081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    10141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    10201 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    10261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    10321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    10381 gtatcattgc agcactgggg tcagatggta agccctcccg tatcgtagtt atctacacga
    10441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    10501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    10561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    10621 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    10681 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    10741 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    10801 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    10861 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    10921 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    10981 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    11041 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    11101 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    11161 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    11221 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    11281 ccagcaacgc ggccttttta cggttcctgg tcttttgctg gccttttgct cacatgttct
    11341 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    11401 ccagcggata acaatttcac a
//
