LOCUS       sequence-243725-        9703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LYS2-URA3 ICE plasmid used to facilitate yeast recombination cloning
            and stable genomic Integration after CEN Excision..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 9703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: pICE LYS2-URA3
FEATURES             Location/Qualifiers
     source          1..9703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     387..404
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    521..542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(557..562,563..580,581..587)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    595..611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     600..622
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     619..635
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     619..635
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     654..674
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        656..674
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    729..1232
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     1263..1279
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1263..1279
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1313..1329)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1314..1330)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1354..1373)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1355..1373)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1383..1400)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1383..1399)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1392..1414)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1540..1995
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1627..1646)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1837..1858
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2052..2291
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             2292..6470
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(6871..6890)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6990..7012
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7050..7068)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7107..7322
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             7323..8126
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        8212..8316
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8317..8385,8386..9177)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8535..8554)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9348..9703,1..233)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
       61 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      301 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      361 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      421 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      481 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      541 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      601 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
      661 accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccgc gccacctggg
      721 tccttttcat cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata
      781 aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga
      841 tgtaaaagac tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc
      901 tcaggtatta atgccgaatt gtttcatctt gtctgtgtag aagaccacac acgaaaatcc
      961 tgtgatttta cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt
     1021 gtctaataaa tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat
     1081 ttttttttct tcattccgta actcttctac cttctttatt tactttctaa aatccaaata
     1141 caaaacataa aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat
     1201 acgaggcgcg tgtaagttac aggcaagcga tccgtcctaa gaagcgctaa gagggcggcc
     1261 gctctagaac tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg
     1321 tcgacctcga gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     1381 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     1441 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     1501 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat
     1561 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     1621 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     1681 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     1741 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     1801 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     1861 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     1921 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     1981 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     2041 ccgcatagat caattccact tgcaattaca taaaaaattc cggcggtttt tcgcgtgtga
     2101 ctcaatgtcg aaatacctgc ctaatgaaca tgaacatcgc ccaaatgtat ttgaagaccc
     2161 gctgggagaa gttcaagata tataagtaac aagcagccaa tagtataaaa aaaaatctga
     2221 gtttattacc tttcctggaa tttcagtgaa aaactgctaa ttatagagag atatcacaga
     2281 gttactcact aatgactaac gaaaaggtct ggatagagaa gttggataat ccaactcttt
     2341 cagtgttacc acatgacttt ttacgcccac aacaagaacc ttatacgaaa caagctacat
     2401 attcgttaca gctacctcag ctcgatgtgc ctcatgatag tttttctaac aaatacgctg
     2461 tcgctttgag tgtatgggct gcattgatat atagagtaac cggtgacgat gatattgttc
     2521 tttatattgc gaataacaaa atcttaagat tcaatattca accaacgtgg tcatttaatg
     2581 agctgtattc tacaattaac aatgagttga acaagctcaa ttctattgag gccaattttt
     2641 cctttgacga gctagctgaa aaaattcaaa gttgccaaga tctggaaagg acccctcagt
     2701 tgttccgttt ggcctttttg gaaaaccaag atttcaaatt agacgagttc aagcatcatt
     2761 tagtggactt tgctttgaat ttggatacca gtaataatgc gcatgttttg aacttaattt
     2821 ataacagctt actgtattcg aatgaaagag taaccattgt tgcggaccaa tttactcaat
     2881 atttgactgc tgcgctaagc gatccatcca attgcataac taaaatctct ctgatcaccg
     2941 catcatccaa ggatagttta cctgatccaa ctaagaactt gggctggtgc gatttcgtgg
     3001 ggtgtattca cgacattttc caggacaatg ctgaagcctt cccagagaga acctgtgttg
     3061 tggagactcc aacactaaat tccgacaagt cccgttcttt cacttatcgc gacatcaacc
     3121 gcacttctaa catagttgcc cattatttga ttaaaacagg tatcaaaaga ggtgatgtag
     3181 tgatgatcta ttcttctagg ggtgtggatt tgatggtatg tgtgatgggt gtcttgaaag
     3241 ccggcgcaac cttttcagtt atcgaccctg catatccccc agccagacaa accatttact
     3301 taggtgttgc taaaccacgt gggttgattg ttattagagc tgctggacaa ttggatcaac
     3361 tagtagaaga ttacatcaat gatgaattgg agattgtttc aagaatcaat tccatcgcta
     3421 ttcaagaaaa tggtaccatt gaaggtggca aattggacaa tggcgaggat gttttggctc
     3481 catatgatca ctacaaagac accagaacag gtgttgtagt tggaccagat tccaacccaa
     3541 ccctatcttt cacatctggt tccgaaggta ttcctaaggg tgttcttggt agacattttt
     3601 ccttggctta ttatttcaat tggatgtcca aaaggttcaa cttaacagaa aatgataaat
     3661 tcacaatgct gagcggtatt gcacatgatc caattcaaag agatatgttt acaccattat
     3721 ttttaggtgc ccaattgtat gtccctactc aagatgatat tggtacaccg ggccgtttag
     3781 cggaatggat gagtaagtat ggttgcacag ttacccattt aacacctgcc atgggtcaat
     3841 tacttactgc ccaagctact acaccattcc ctaagttaca tcatgcgttc tttgtgggtg
     3901 acattttaac aaaacgtgat tgtctgaggt tacaaacctt ggcagaaaat tgccgtattg
     3961 ttaatatgta cggtaccact gaaacacagc gtgcagtttc ttatttcgaa gttaaatcaa
     4021 aaaatgacga tccaaacttt ttgaaaaaat tgaaagatgt catgcctgct ggtaaaggta
     4081 tgttgaacgt tcagctacta gttgttaaca ggaacgatcg tactcaaata tgtggtattg
     4141 gcgaaatagg tgagatttat gttcgtgcag gtggtttggc cgaaggttat agaggattac
     4201 cagaattgaa taaagaaaaa tttgtgaaca actggtttgt tgaaaaagat cactggaatt
     4261 atttggataa ggataatggt gaaccttgga gacaattctg gttaggtcca agagatagat
     4321 tgtacagaac gggtgattta ggtcgttatc taccaaacgg tgactgtgaa tgttgcggta
     4381 gggctgatga tcaagttaaa attcgtgggt tcagaatcga attaggagaa atagatacgc
     4441 acatttccca acatccattg gtaagagaaa acattacttt agttcgcaaa aatgccgaca
     4501 atgagccaac attgatcaca tttatggtcc caagatttga caagccagat gacttgtcta
     4561 agttccaaag tgatgttcca aaggaggttg aaactgaccc tatagttaag ggcttaatcg
     4621 gttaccatct tttatccaag gacatcagga ctttcttaaa gaaaagattg gctagctatg
     4681 ctatgccttc cttgattgtg gttatggata aactaccatt gaatccaaat ggtaaagttg
     4741 ataagcctaa acttcaattc ccaactccca agcaattaaa tttggtagct gaaaatacag
     4801 tttctgaaac tgacgactct cagtttacca atgttgagcg cgaggttaga gacttatggt
     4861 taagtatatt acctaccaag ccagcatctg tatcaccaga tgattcgttt ttcgatttag
     4921 gtggtcattc tatcttggct accaaaatga tttttacctt aaagaaaaag ctgcaagttg
     4981 atttaccatt gggcacaatt ttcaagtatc caacgataaa ggcctttgcc gcggaaattg
     5041 acagaattaa atcatcgggt ggatcatctc aaggtgaggt cgtcgaaaat gtcactgcaa
     5101 attatgcgga agacgccaag aaattggttg agacgctacc aagttcgtac ccctctcgag
     5161 aatattttgt tgaacctaat agtgccgaag gaaaaacaac aattaatgtg tttgttaccg
     5221 gtgtcacagg atttctgggc tcctacatcc ttgcagattt gttaggacgt tctccaaaga
     5281 actacagttt caaagtgttt gcccacgtca gggccaagga tgaagaagct gcatttgcaa
     5341 gattacaaaa ggcaggtatc acctatggta cttggaacga aaaatttgcc tcaaatatta
     5401 aagttgtatt aggcgattta tctaaaagcc aatttggtct ttcagatgag aagtggatgg
     5461 atttggcaaa cacagttgat ataattatcc ataatggtgc gttagttcac tgggtttatc
     5521 catatgccaa attgagggat ccaaatgtta tttcaactat caatgttatg agcttagccg
     5581 ccgtcggcaa gccaaagttc tttgactttg tttcctccac ttctactctt gacactgaat
     5641 actactttaa tttgtcagat aaacttgtta gcgaagggaa gccaggcatt ttagaatcag
     5701 acgatttaat gaactctgca agcgggctca ctggtggata tggtcagtcc aaatgggctg
     5761 ctgagtacat cattagacgt gcaggtgaaa ggggcctacg tgggtgtatt gtcagaccag
     5821 gttacgtaac aggtgcctct gccaatggtt cttcaaacac agatgatttc ttattgagat
     5881 ttttgaaagg ttcagtccaa ttaggtaaga ttccagatat cgaaaattcc gtgaatatgg
     5941 ttccagtaga tcatgttgct cgtgttgttg ttgctacgtc tttgaatcct cccaaagaaa
     6001 atgaattggc cgttgctcaa gtaacgggtc acccaagaat attattcaaa gactacttgt
     6061 atactttaca cgattatggt tacgatgtcg aaatcgaaag ctattctaaa tggaagaaat
     6121 cattggaggc gtctgttatt gacaggaatg aagaaaatgc gttgtatcct ttgctacaca
     6181 tggtcttaga caacttacct gaaagtacca aagctccgga actagacgat aggaacgccg
     6241 tggcatcttt aaagaaagac accgcatgga caggtgttga ttggtctaat ggaataggtg
     6301 ttactccaga agaggttggt atatatattg catttttaaa caaggttgga tttttacctc
     6361 caccaactca taatgacaaa cttccactgc caagtataga actaactcaa gcgcaaataa
     6421 gtctagttgc ttcaggtgct ggtgctcgtg gaagctccgc agcagcttaa ggttgagcat
     6481 tacgtatgat atgtccatgt acaataatta aatatgaatt aggagaaaga cttagcttct
     6541 tttcgggtga tgtcacttaa aaactccgag aataatatat aataagagaa taaaatatta
     6601 gttattgaat aagaactgta aatcagctgg cgttagtctg ctaatggcag cttcatcttg
     6661 gtttattgta gcatgaatca tatttgcctt tttttcctgt aattcaatga ttcttgcttc
     6721 tatactatcc tcaatgcaaa accttgtgat cttcacaggt cgatactgac caattctatg
     6781 aactctatca ccactttgcc attcaacact agggttccac catgggtcta aaatgaatac
     6841 ttgcgaagct gatctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     6901 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     6961 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     7021 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     7081 tgtcatgatg caccatacca cagcttttca attcatcatt ttttttttat tctttttttt
     7141 gatttcggtt tccttgaaat ttttttgatt cggtaatctc cgaacagaag gaagaacgaa
     7201 ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa gaaacatgaa
     7261 attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa cgaagataaa
     7321 tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct gttgctgcca
     7381 agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta
     7441 ccaccaagga attactggag ttagttgaag cattaggtcc caaaatttgt ttactaaaaa
     7501 cacatgtgga tatcttgact gatttttcca tggagggcac agttaagccg ctaaaggcat
     7561 tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac attggtaata
     7621 cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca gacattacga
     7681 atgcacacgg tgtggtgggc ccaggtattg ttagcggttt gaagcaggcg gcagaagaag
     7741 taacaaagga acctagaggc cttttgatgt tagcagaatt gtcatgcaag ggctccctat
     7801 ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa gattttgtta
     7861 tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat tggttgatta
     7921 tgacacccgg tgtgggttta gatgacaagg gagacgcatt gggtcaacag tatagaaccg
     7981 tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga ctatttgcaa
     8041 agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg gaagcatatt
     8101 tgagaagatg tggccagcaa aactaaaaaa ctgtattata agtaaatgca tgtatactaa
     8161 actcacaaat tagagcttca gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     8221 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8281 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8341 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8401 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     8461 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     8521 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     8581 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     8641 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8701 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     8761 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8821 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     8881 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     8941 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9001 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9061 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9121 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9181 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9241 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9301 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9361 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9421 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9481 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9541 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9601 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9661 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaa
//
