LOCUS       sequence-243727-       10745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fusion of disordered protein TAF15(1-208), fluorescent 
            protein mCherry, and SspB which upon light activation binds to 
            iLID..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10745)
  AUTHORS   Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen
            NS, Haataja M, Brangwynne CP
  TITLE     Liquid Nuclear Condensates Mechanically Sense and Restructure the 
            Genome.
  JOURNAL   Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 
            10.1016/j.cell.2018.10.057.
  PUBMED    30500535
REFERENCE   2  (bases 1 to 10745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.10"; journalName: "Cell"; date: "2018-11-29- 
            29"; volume: "175"; issue: "6"; pages: "1481-1491.e13"
COMMENT     Sequence Label: pHR-pSFFV-TAF15N-mCherry-sspB
FEATURES             Location/Qualifiers
     source          1..10745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1854..2183
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     misc_feature    2290..2878
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2343..2363)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2761..2772)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2881..2897)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2882..2898)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2988..3221
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3246..3264)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3247..3264)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3554..3573)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3787..3806)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3906..3928
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3966..3984)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4052..4156
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4157..4225,4226..5017)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4375..4394)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5188..5776
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5677..5696
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5930..5947
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6017..6037)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6022..6351
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6202..6337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7485..7550
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7680..7700
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8125..8259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8162..8181)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8216..8235
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8427..9060
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9107..9232
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9729..9962
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10147..10191
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10476..10593
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag
      421 ctctcgagaa ttctcacgcg tcaagtggag caaggcaggt ggacagtgga tccggagcta
      481 ccatgtcgga ttctggaagt tacggtcagt ctgggggtga gcagcaaagt tattctacct
      541 atggaaatcc aggcagccaa ggctatggac aagcatcaca aagctattct ggctatgggc
      601 aaacgactga ttcctcttat ggacagaact acagcggtta ctccagttat ggacaaagtc
      661 agtcaggtta ttcacagtcc tatggtggtt atgagaatca aaagcagagc tcatatagcc
      721 agcaaccata taataaccag ggacagcagc aaaacatgga atcatcagga agccaaggtg
      781 gaagagcacc ttcctatgac cagccagact atggtcaaca agattcatat gaccagcagt
      841 caggctatga tcaacatcaa ggctcatatg atgagcagtc aaattatgat cagcagcatg
      901 attcctatag tcaaaaccag cagtcctatc attcacaaag ggaaaactac agccaccaca
      961 cacaagatga ccgtcgtgat gtgagtaggt atggagaaga taatagagga tatggcgggt
     1021 cacagggagg aggtagaggg cgtgggggat atgacaagga tggaagaggt cctatgacag
     1081 gatcaagtgg tggtgaccgc ggtggcggtg gaggaatggt gtctaaaggc gaggaggata
     1141 acatggctat aatcaaggaa tttatgaggt tcaaggtgca tatggaggga tctgtgaacg
     1201 gtcacgagtt cgaaatcgag ggcgaaggcg aggggcgacc ctatgaaggg acacagacgg
     1261 ccaaactgaa ggtgaccaag ggtggccccc tgcctttcgc ctgggacatc ctgtcacccc
     1321 agtttatgta cggctctaag gcttacgtta agcaccctgc cgatattccg gactacttga
     1381 aactgagctt ccctgaaggg ttcaagtggg aacgggtaat gaattttgag gatggcggtg
     1441 tagtcacagt tacccaggac agctctcttc aggacggaga atttatctat aaggttaaac
     1501 ttcggggcac taacttccca tcagacggcc ccgtgatgca gaagaaaact atgggctggg
     1561 aagccagctc agaacgcatg tatcccgagg acggagcact gaagggagaa atcaagcagc
     1621 gactcaagct gaaggatggg ggacattatg atgctgaggt caaaaccacc tataaggcca
     1681 agaaacccgt tcagcttcct ggggcataca acgtgaatat caaactggat attacatccc
     1741 ataacgaaga ctataccatc gtggaacagt acgagcgggc cgagggcaga catagcacag
     1801 ggggcatgga tgaattgtac aaggcggccg gatccggtgg tacctctgga ggtagctccc
     1861 cgaaacgccc taagctgctg cgtgaatatt acgattggct ggttgataac agctttaccc
     1921 catatctggt ggtggatgcc acatacctgg gcgtgaacgt gcccgtggag tatgtgaaag
     1981 acggtcagat cgtgctgaat ctgtctgcaa gtgcgaccgg caacctgcaa ctgacaaatg
     2041 attttatcca gttcaacgcc cgctttaagg gcgtgtctcg tgaactgtat atcccgatgg
     2101 gtgccgctct ggccatttac gctcgcgaga acggcgatgg tgtgatgttc gaaccagaag
     2161 aaatctatga cgagctgaat attggttaag gatcggccgc aactcccacc tgcaacatgc
     2221 gtgactgact gaggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag
     2281 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     2341 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     2401 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     2461 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     2521 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     2581 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     2641 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     2701 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     2761 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     2821 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     2881 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
     2941 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     3001 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     3061 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     3121 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     3181 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     3241 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     3301 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     3361 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     3421 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     3481 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     3541 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     3601 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     3661 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     3721 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     3781 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     3841 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     3901 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     3961 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     4021 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4081 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4141 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4201 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4261 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4321 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     4381 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4441 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4501 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4561 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4621 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4681 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4741 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4801 aggaacactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4861 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4921 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4981 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5041 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5101 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5161 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5221 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5281 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     5341 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5401 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5461 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5521 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5581 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5641 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5701 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5761 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5821 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5881 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     5941 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6001 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     6061 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     6121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     6181 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6241 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6301 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
     6361 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
     6421 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     6481 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     6541 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     6601 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     6661 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     6721 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     6781 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
     6841 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     6901 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
     6961 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     7021 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
     7081 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     7141 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
     7201 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     7261 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
     7321 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
     7381 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg
     7441 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     7501 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     7561 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     7621 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     7681 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     7741 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     7801 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     7861 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     7921 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     7981 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     8041 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     8101 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     8161 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8221 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     8281 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     8341 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     8401 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
     8461 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     8521 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     8581 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     8641 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     8701 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     8761 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     8821 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     8881 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8941 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9001 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9061 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     9121 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     9181 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     9241 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     9301 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     9361 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     9421 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     9481 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     9541 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
     9601 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     9661 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     9721 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     9781 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     9841 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     9901 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     9961 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    10021 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    10081 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    10141 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    10201 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    10261 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    10321 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    10381 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
    10441 cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
    10501 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
    10561 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
    10621 ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa taaaagattt
    10681 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
    10741 ctgca
//
