LOCUS       sequence-243744-        9271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral plasmid to co-express a guide RNA and 
            CyOFP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Giuliano CJ, Lin A, Girish V, Sheltzer JM
  TITLE     Generating Single Cell-Derived Knockout Clones in Mammalian Cells 
            with CRISPR/Cas9.
  JOURNAL   Curr Protoc Mol Biol. 2019 Sep;128(1):e100. doi: 10.1002/cpmb.100.
  PUBMED    31503414
REFERENCE   2  (bases 1 to 9271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol."; date: "2019-09"; volume: "128(1):e100. doi"; pages: " 
            10.1002/cpmb.100"
COMMENT     Sequence Label: LRG2.1-CyOFP1
FEATURES             Location/Qualifiers
     source          1..9271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     24..41
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    158..179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(194..199,200..217,218..224)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    232..248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     237..259
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     256..272
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     256..272
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     291..311
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        293..311
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        339..565
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             566..746
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    793..918
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1411..1644
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1829..1873
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2051..2299
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2051..2071
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2222..2241
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    4325..4442
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4511..4722
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4742..5443
                     /codon_start=1
                     /product="orange-red fluorescent protein that is excitable 
                     by cyan light and suitable as an acceptor for blue-emitting
                     luciferase (Chu et al., 2016)"
                     /label=CyOFP1
                     /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTN
                     RIKVVEGGPLPFAFDILATHFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
                     VLTATQDTSLQDGELIYNVKVRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
                     KALKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
                     SNLGGGMDELYK"
     misc_feature    5462..6050
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5515..5535)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5933..5944)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6122..6355
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6433..6554
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6470..6489)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6524..6543
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6594..6729
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6749..6768)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6750..6768)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6778..6795)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6778..6794)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6787..6809)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6936..7391
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7023..7042)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7233..7254
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7417..7521
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7522..7590,7591..8382)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7740..7759)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8553..9141
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9042..9061
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
       61 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      121 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      181 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      241 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
      301 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
      361 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
      421 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
      481 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
      541 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
      601 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      661 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
      721 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
      781 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
      841 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
      901 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
      961 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     1021 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     1081 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     1141 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     1201 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     1261 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     1321 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     1381 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     1441 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     1501 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     1561 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     1621 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     1681 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     1741 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     1801 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     1861 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     1921 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     1981 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     2041 gggacccaga gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
     2101 tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
     2161 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
     2221 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
     2281 gtggaaagga cgaaacaccg gagacggttg taaatgagca cacaaaatac acatgctaaa
     2341 atattatatt ctatgacctt tataaaatca accaaaatct tctttttaat aactttagta
     2401 tcaataatta gaatttttat gttccttttt gcaaactttt aataaaaatg agcaaaataa
     2461 aaaaacgcta gttttagtaa ctcgcgttgt tttcttcacc tttaataata gctactccac
     2521 cacttgttcc taagcggtca gctcctgctt caatcatttt ttgagcatct tcaaatgttc
     2581 taactccacc agctgcttta actaaagcat tgtctttaac aactgacttc attagtttaa
     2641 catcttcaaa tgttgcacct gattttgaaa atcctgttga tgttttaaca aattctaatc
     2701 cagcttcaac agctatttca caagctttca tgatttcttc ttttgttaat aaacaatttt
     2761 ccataataca tttaacaaca tgtgatccag ctgctttttt tacagctttc atgtcttcta
     2821 aaactaattc ataatttttg tcttttaatg caccaatatt taataccata tcaatttctg
     2881 ttgcaccatc tttaattgct tcagaaactt cgaatgcttt tgtagctgtt gtgcatgcac
     2941 ctagaggaaa acctacaaca tttgttattc ctacatttgt gccttttaat aattctttac
     3001 aatagcttgt tcaatatgaa ttaacacaaa ctgttgcaaa atcaaattca attgcttcat
     3061 cacataattg tttaatttca gctttcgtag catcttgttt taataatgtg tgatctatat
     3121 atttgtttag tttcattttt tctcctatat attcattttt aattttaatt ctttaataat
     3181 ttcgtctact ttaactttag cgttttgaac agattcacca acacctataa aataaatttt
     3241 tagtttaggt tcagttccac ttgggcgaac agcaaatcat gacttatctt ctaaataaaa
     3301 ttttagtaag tcttgtcctg gcatattata cattccatcg atgtagtctt caacattaac
     3361 aactttaagt ccagcaattt gagttaaggg tgttgctctc aatgatttca ttaatggttc
     3421 aatttttaat ttcttttctt ctggtttaaa attcaagttt aaagtgaaag tgtaatatgc
     3481 acccatttct ttaaataaat cttctaaata gtctactaat gttttatttt gttttttata
     3541 aaatcaagca gcctctgcta ttaatataga agcttgtatt ccatctttat ctctagctga
     3601 gtcatcaatt acatatccat aactttcttc ataagcaaaa acaaaattta atccgttatc
     3661 ttcttcttta gcaatttctc tacccattca tttaaatcca gttaaagttt ttacaatatt
     3721 aactccatat ttttcatgag cgattctatc acccaaatca cttgttacaa aacttgaata
     3781 tagagccgga ttttttggaa tgctatttaa gcgttttaga tttgataatt ttcaatcaat
     3841 taaaattggt cctgtttgat ttccatctaa tcttacaaaa tgaccatcat gttttattgc
     3901 cattccaaat ctgtcagcat ctgggtcatt cataataata atatctgcat catgtttaat
     3961 accatattca agcggtattt ttcatgcagg atcaaattct ggatttggat ttacaacatt
     4021 tttaaatgtt tcatcttcaa atgcatgctc ttcaacctca ataacgttat atcctgattc
     4081 acgtaatatt tttggggtaa atttagttcc tgttccatta actgcgctaa aaataatttt
     4141 taaatctttt ttagcttctt gctctttttt gtacgtctct gtttaagagc tatgctggaa
     4201 acagcatagc aagtttaaat aaggctagtc cgttatcaac tttgctggaa acagcaaagt
     4261 ggcaccgagt cggtgctttt tttgaattct agatcttgag acaaatggca gtattcatcc
     4321 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     4381 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     4441 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag tggctccggt
     4501 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     4561 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     4621 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     4681 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtgtcgtg acgcgggatc
     4741 catggtgagc aagggcgagg agctgatcaa ggagaacatg agaagcaagc tgtacctgga
     4801 aggcagcgtg aacggccacc agttcaagtg cacccacgaa ggggagggca agccctacga
     4861 gggcaagcag accaacagga tcaaggtggt ggagggaggc cccctgccgt tcgcattcga
     4921 catcctggcc acccacttta tgtacgggag caaggtgttc atcaagtacc ccgccgacct
     4981 ccccgattat tttaagcagt ccttccctga gggcttcaca tgggagagag tcatggtgtt
     5041 cgaagacggg ggcgtgctga ccgccaccca ggacaccagc ctccaggacg gcgagctcat
     5101 ctacaacgtc aaggtcagag gggtgaactt cccagccaac ggccccgtga tgcagaagaa
     5161 aacactgggc tgggagccca gcaccgagac catgtacccc gctgacggcg gcctggaagg
     5221 cagatgcgac aaggccctga agctcgtggg cgggggccac ctgcacgtca acttcaagac
     5281 cacatacaag tccaagaaac ccgtgaagat gcccggcgtc cactacgtgg accgcagact
     5341 ggaaagaatc aaggaggccg acaacgagac ctacgtcgag cagtacgagc acgctgtggc
     5401 cagatactcc aacctgggcg gaggcatgga cgagctgtac aagtagacgc gttaagtcga
     5461 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5521 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5581 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     5641 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     5701 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     5761 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     5821 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     5881 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     5941 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6001 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     6061 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6121 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     6181 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6241 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6301 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg
     6361 tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6421 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6481 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6541 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     6601 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6661 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     6721 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     6781 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     6841 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     6901 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     6961 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     7021 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     7081 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     7141 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     7201 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     7261 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     7321 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     7381 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7441 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7501 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7561 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7621 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7681 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7741 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     7801 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     7861 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7921 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7981 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     8041 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     8101 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     8161 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8221 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8281 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8341 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8401 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     8461 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8521 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8581 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8641 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     8701 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8761 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     8821 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8881 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8941 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9001 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9061 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9121 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9181 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9241 attaccgcct ttgagtgagc tgataccgct c
//
