LOCUS       sequence-24375-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  TITLE     T4 Lysozyme Plasmids - Tray 5
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme L133F WT*
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(710..732)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    721..737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(745..751,752..767,768..772))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 tacgaaaagc ccgctcatta ggcgggctca gatctgctca tgtttgacag cttatcatcg
       61 ataagcttcg gcaataaatt cttgatatgt tttcatatgt ttcctttaaa tattttaatt
      121 agtaattgtc tattcaagta attgtgaata tactatcaca attccaagag aaagtaaaca
      181 gctttataga tttttatacg cgtcccaagt gccagttcta aacgttgtaa tgactcgttt
      241 tgcgcgatta ggtgtttgat tataccatct agatttagca aagttaactg ctgcttcatc
      301 ccagcgtttt tgttgaagca tacgtaaaga gttagtaaat cctgccacac cggtttctcc
      361 catttggaaa accatattaa tcaatgcagc gcgacgaacc gcatcaagag aatcataaac
      421 cggttttaat ttagcatttc tcagaattcc gcgaacagca gcatcaacat cctgattaaa
      481 gagtttttca gcctcatctt ttgtaattac accattagta ttacgcccaa tagctttatc
      541 taattcagat ttagcagcat taagtgatgg actttttgta agcaaatgac cgatgccaat
      601 agtgtaatag ccttctgtgt ctttatagat tttaagtcta agaccttcat ctatacgtaa
      661 catttcaaat atattcataa tacctcctaa gcatcgatgg atcctgtttc ctgtgtgaaa
      721 ttgttatccg ctcacaattc cacacattat acgagccgat gattaattgt cacaggggga
      781 tggggagtag ctgatcctgt tc
//
