LOCUS       sequence-243751-        7479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Chih B, Gollan L, Scheiffele P
  TITLE     Alternative splicing controls selective trans-synaptic interactions 
            of the neuroligin-neurexin complex.
  JOURNAL   Neuron. 2006 Jul 20. 51(2):171-8.
  PUBMED    16846852
REFERENCE   2  (bases 1 to 7479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2006 Jul 20. 51(2):171-8."
COMMENT     Sequence Label: pCAG-NL1AB
FEATURES             Location/Qualifiers
     source          1..7479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          646..1662
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1586..1608
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1670..1689
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1907..1933
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      2389..2398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4359..4368
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4483..4502)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4548..4603
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4602..4621)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4964..4980)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4964..4980)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4977..4999)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4988..5004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5012..5018,5019..5036,5037..5042))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5057..5078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5137..5332
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5183..5318
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5338..5472
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5375..5394)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5429..5448
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5540..5557)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5711..6299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5791..5810)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6470..7261,7262..7330))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7093..7112
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7331..7435)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(7466..7479,1..366)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      481 gggggggggc gcgcgccrgg sggggsgggg sggggsgrgg ggsggggsgg ggsgrggsgg
      541 rgrggkgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc
      661 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
      721 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
      781 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
      841 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg
      901 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
      961 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
     1021 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga
     1081 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt
     1141 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc
     1201 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
     1261 ggggrgggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg
     1321 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
     1381 tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc
     1441 ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
     1501 ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc
     1561 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
     1621 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     1681 gttattgtgc tgtctcatca ttttggcaaa gaattcgcgg ccgctagcgc taccggactc
     1741 agatctcgag ctcaagcttg ggaccatggc acttcccaga tgcatgtggc caaattatgt
     1801 ttggagagct atgatggcat gtgtggtcca caggggatcc ggtgccccat tgactctctg
     1861 cttgttggga tgtttgctac agacttttca cgtactctct caaaagtatc catacgacgt
     1921 tccggactac gcattggatg atgtagaccc attggttact actaactttg gcaagattag
     1981 gggaattaag aaagaactca ataatgaaat tttgggtcct gtcattcagt ttcttggggt
     2041 tccatatgcc gctccaccaa caggagaaca tcgtttccag cctccagaac caccatctcc
     2101 ctggtctgac atccggaacg ccactcagtt tgctcctgta tgtccccaga atatcattga
     2161 tggcagattg cctgaagtta tgcttcctgt gtggttcact aataacttgg atgtggtttc
     2221 atcatacgtc caagaccaga gtgaagactg tctatactta aacatctatg tcccaactga
     2281 agatggtccc cttacaaaga aacacacaga tgatttaggt gataatgacg gtgctgaaga
     2341 tgaagatatt cgggacagtg ggggtcccaa accagtgatg gtgtacatcc atggcggctc
     2401 ttacatggaa ggtactggaa atctgtatga tgggagtgtc ttggcaagct atggcaatgt
     2461 gatcgtcatc acagtcaact atcggcttgg ggtacttggc ttcttgagca caggggatca
     2521 ggctgccaaa ggaaactacg ggctccttga cctcatccag gccctaagat ggaccagcga
     2581 gaacattggg ttctttggtg gtgacccctt gcgaatcact gtgtttggat caggcgctgg
     2641 gggttcatgt gtcaacctgc tgactttatc ccattattct gaaggtaacc gttggagcaa
     2701 ttcaaccaaa ggactttttc aacgagcaat agctcagagt ggaacagccc tttccagctg
     2761 ggctgttagt ttccagcctg caaaatacgc tagaattctg gccacaaaag ttggctgcaa
     2821 tgtttcagat acagtagagt tagtagaatg cctgcagaag aagccttaca aagaacttgt
     2881 tgatcaagat gttcaaccag cccgatacca catagccttt ggacctgtga tcgatggtga
     2941 tgtaatacca gatgaccctc agatactgat ggaacaagga gagttcctca actatgatat
     3001 aatgttggga gttaaccaag gggaagggtt gaagtttgtc gaaaatatag tagatagtga
     3061 tgatggtgta tcagccagtg atttcgactt tgctgtttct aattttgttg ataatttata
     3121 tggatatcct gaaggcaaag atgttttgag agaaaccatt aaattcatgt atactgactg
     3181 ggctgatcgc cataatcctg aaactagaag gaagacattg ttggctttgt ttacggacca
     3241 tcaatgggta gcacctgctg tggccacagc agaccttcac tcgaactttg gctcacctac
     3301 atacttctat gccttttatc atcattgcca aacagatcaa gttccagctt gggctgatgc
     3361 agctcatggg gatgaggttc cctatgtgtt gggaatcccc atgattggcc ctacagagtt
     3421 atttccttgc aatttctcca agaatgatgt gatgttgagt gcagtagtaa tgacatactg
     3481 gacgaatttt gctaaaactg gtgacccaaa tcaaccagtt cctcaagaca caaaattcat
     3541 ccataccaaa cccaaccgct ttgaagaagt agcatggacc agatattccc agaaagacca
     3601 gctttatctc catattggat taaaaccgag agttaaagag cattacagag ccaataaggt
     3661 aaatctctgg ctggagctgg tacctcatct gcataatctc aatgacattt ctcagtatac
     3721 ctcgacaaca actaaagtgc catccacgga catcactctc agacctacaa ggaagaattc
     3781 cacaccagtc acatcagcct ttcccactgc caaacaggat gatcccaagc aacaaccaag
     3841 ccccttctcg gtggatcaga gggactactc cacagagcta agtgtcacta tcgcagtggg
     3901 ggcctctctg ctgtttctca acatcttggc ttttgcagcc ctgtactaca agaaggataa
     3961 gaggagacat gatgtccacc ggaggtgcag ccctcagcgc acgaccacca acgacctaac
     4021 ccatgctcca gaagaggaaa ttatgtctct ccaaatgaag cacactgact tggatcacga
     4081 gtgtgagtcc atccatccac atgaggtggt tcttcggacc gcctgtcccc cagattatac
     4141 tctagctatg aggaggtcac ctgatgatat tccactaatg acacctaaca ccatcacaat
     4201 gattcccaac actataccag ggattcagcc cttacataca ttcaacacat ttactggagg
     4261 acagaataat acactgcccc atccccaccc acacccccat tcacattcaa caaccagggt
     4321 atagccagat gagagagaca aactgattaa tcccgcggcc atggcggccg ggagcatgcg
     4381 acgtcgggcc cggggagctc aattcactcc tcaggtgcag gctgcctatc agaaggtggt
     4441 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
     4501 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     4561 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     4621 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     4681 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     4741 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     4801 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     4861 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     4921 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
     4981 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     5041 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     5101 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat
     5161 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5221 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5281 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
     5341 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5401 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5461 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5521 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5581 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5641 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5701 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5761 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5821 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5881 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     5941 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6001 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6061 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6121 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     6181 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6241 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6301 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6361 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6421 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6481 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6541 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     6601 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     6661 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6721 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6781 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6841 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     6901 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     6961 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7021 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7081 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7141 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7201 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7261 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7321 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7381 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7441 ttccccgaaa agtgccacct gggtcgacat tgattattg
//
