LOCUS       sequence-243795-       11043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector encoding BFP-BSD-HA-Dmap1 resistant against gRNA 
            encoded by pKLV2-U6(gDmap1 v4)--PGK-Puro-BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11043)
  AUTHORS   Yu JSL, Palano G, Lim C, Moggio A, Drowley L, Plowright AT, 
            Bohlooly-Y M, Rosen BS, Hansson EM, Wang QD, Yusa K
  TITLE     CRISPR-KO screen identifies Dmap1 as a regulator of 
            chemically-induced reprogramming and differentiation of cardiac 
            progenitors.
  JOURNAL   Stem Cells. 2019 Apr 1. doi: 10.1002/stem.3012.
  PUBMED    30932271
REFERENCE   2  (bases 1 to 11043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2019 Apr 1. doi: 10.1002/stem.3012."
COMMENT     Sequence Label: pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Dmap1(SDM)
FEATURES             Location/Qualifiers
     source          1..11043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        102..300
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     255..275
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             318..498
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    545..670
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1169..1402
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1587..1631
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1929..2046
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2121..2369
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2121..2141
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2292..2311
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2903..4084
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3134..4076
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4033..4053
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4097..4106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4103..4804
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     CDS             4823..4876
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4883..5278
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             5297..5350
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5357..5383
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6860..6880)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7278..7289)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7741..7758)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7747..7971
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7997..8017)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7997..8015)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8036..8052)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8036..8052)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8049..8071)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8060..8076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8084..8090,8091..8108,8109..8114))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8129..8150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8267..8284)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8438..9026)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8518..8537)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9197..9988,9989..10057))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9820..9839
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10058..10162)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10188..10643)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10325..10346)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10537..10556
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10770..10792
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10784..10801
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10785..10801
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10811..10830
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10811..10829
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
       61 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      121 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      181 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      241 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      301 cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      361 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      421 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      481 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
      541 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      601 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      661 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
      721 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
      781 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
      841 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
      901 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
      961 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgcgc
     1021 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1081 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1141 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1201 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1261 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1321 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1381 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1441 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1501 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1561 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1621 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1681 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     1741 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     1801 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     1861 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     1921 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1981 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2041 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggccctacg
     2101 cgtccaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat ttgcatatac
     2161 gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag
     2221 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
     2281 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
     2341 tatatatctt gtggaaagga cgaaacaccg gagacgcatg actcgtctca gtttaagagc
     2401 tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt
     2461 ggcaccgagt cggtgctttt tttacgcgtg aggggcgcgg aagccggggc ggaggaggag
     2521 ccaaccccgg gctgggctga gacccgcaga ggaagacgct ctagggattt gtcccggact
     2581 agcgagatgg caaggctgag gacgggaggc tgattgagag gcgaaggtac accctaatct
     2641 caatacaacc tttggagcta agccagcaat ggtagaggga agattctgca cgtcccttcc
     2701 aggcggcctc cccgtcacca ccccccccaa cccgccccga ccggagctga gagtaattca
     2761 tacaaaagga ctcgcccctg ccttggggaa tcccagggac cgtcgttaaa ctcccactaa
     2821 cgtagaaccc agagatcgct gcgttcccgc cccctcaccc gcccgctctc gtcatcactg
     2881 aggtggagaa gagcatgcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc
     2941 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaagtg
     3001 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     3061 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     3121 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     3181 tggcccttgc gtgccttgaa ttacttccac gcccctggct gcagtacgtg attcttgatc
     3241 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     3301 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     3361 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     3421 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     3481 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     3541 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     3601 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     3661 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     3721 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     3781 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     3841 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     3901 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     3961 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     4021 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     4081 cgtggaagct tggcgcgcca ccatgggcag cgagctgatt aaggagaaca tgcacatgaa
     4141 gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg
     4201 caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc
     4261 cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca
     4321 cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca catgggagag
     4381 agtcaccaca tacgaggacg ggggcgtgct gaccgctacc caggacacca gcctccagga
     4441 cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt
     4501 gatgcagaag aaaacactcg gctgggaggc cttcaccgag accctgtacc ccgctgacgg
     4561 cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc
     4621 aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt
     4681 ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga cctacgtcga
     4741 gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct
     4801 taatggaggg ggcagcggcg gggaaggaag gggctccctg ctcacctgcg gcgacgtgga
     4861 agagaacccc ggacctgtcg acatggccaa gcctttgtct caagaagaat ccaccctcat
     4921 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     4981 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     5041 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     5101 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     5161 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     5221 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcgg
     5281 agggggcagc ggcggggaag gaaggggctc cctgctcacc tgcggcgacg tggaagagaa
     5341 ccccggacct atgcattacc catacgatgt tccagattac gctatggcta cgggcgcaga
     5401 tgtacgagac attctagaac tcgggggtcc agagggagat gccgcctctg ggaccatcag
     5461 caaaaaggat attatcaacc cggacaagaa aaagtccaag aagtcctcag agacgctgac
     5521 cttcaagagg cctgagggca tgcatcggga ggtctatgct ttgctttact ctgacaaaaa
     5581 ggatgcaccc ccactgctgc ccagtgacac tggtcagggg tatcggacag tgaaggcgaa
     5641 actggggtcc aagaaggttc gcccttggaa atggatgccc ttcaccaatc ccgcccgcaa
     5701 ggacggcgct atgtttttcc actggcgacg agcggcggag gagggcaagg actacccttt
     5761 tgccaggttc aataagacgg tgcaggtgcc cgtgtactca gagcaggagt accaactcta
     5821 ccttcatgat gacgcatgga ctaaggcaga gactgaccac ctatttgacc tcagccgccg
     5881 atttgatctg cgcttcgtag ttattcacga tcggtatgac caccagcagt tcaagaagcg
     5941 ttctgtggag gacctgaaag agaggtacta ccacatttgt gccaagcttg ccaacgtgag
     6001 ggctgtgcca ggcacagatc tcaagatacc agtgtttgat gctgggcatg agagacggcg
     6061 gaaggaacag ctagagcggc tttacaaccg aaccccagag caggtggcag aggaggagta
     6121 cctcctacag gagctgcgta agattgaggc ccggaaaaaa gagcgggaga agcgcagcca
     6181 agacctgcag aagctgatta cagcagcaga caccactgca gagcagcggc gcacggaacg
     6241 caaggctccc aagaagaagc taccccaaaa gaaggaggct gagaagccgg ctgtccctga
     6301 gactgcaggc atcaagtttc cagattttaa gtcggcaggt gtcacgctac ggagccagcg
     6361 gatgaagcta cccagctctg tgggtcagaa gaagatcaag gcgctggaac agatgctgct
     6421 ggaacttggt gtggagctga gccctacccc cacagaggag ctggtgcata tgttcaatga
     6481 gttgcggagc gacctggtgt tactctacga gctcaagcag gcctgtgcca actgtgaata
     6541 tgagctacag atgctgcggc accggcacga ggccctggct cgggcaggag tgctgggggc
     6601 ccctgccgca gcagcagtgg gaccaacccc ggcttctgct gagccaacag tgtctgaatc
     6661 tggacttggt ctggacccca ccaaggatac catcattgat gtcgtgggtg cacccctcac
     6721 acccaattcg cggaaacgac gggaatcagc ctccagctca tcttctgtga agaaagccaa
     6781 gaaaccataa ggatccgcta gcggccgctc aacctctgga ttacaaaatt tgtgaaagat
     6841 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6901 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6961 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7021 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7081 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7141 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7201 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7261 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7321 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7381 gggccgcctc cccgcaggta cctttaagac caatgactta caaggcagct gtagatctta
     7441 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa agaagtcaag
     7501 atctgctttt tgcctgtact gggtctctct ggttagacca gagtctctct ggttagacca
     7561 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7621 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7681 atccctcaga cccttttagt cagtgtggaa aatctctagc agtttaaacc cgctgatcag
     7741 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     7801 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     7861 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     7921 aggattggga agtcaatagc aggcatgctg gggatgcggt gggctctatg ggcggccgtt
     7981 aattaagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     8041 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggga
     8101 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8161 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8221 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8281 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8341 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8401 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8461 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8521 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8581 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8641 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8701 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8761 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8821 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     8881 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8941 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9001 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9061 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9121 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9181 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9241 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9301 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9361 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9421 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9481 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9541 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9601 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9661 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9721 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9781 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9841 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9901 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9961 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10021 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10081 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10141 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
    10201 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
    10261 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
    10321 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
    10381 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
    10441 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
    10501 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
    10561 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
    10621 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
    10681 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    10741 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    10801 tgagcgcgcg taatacgact cactataggg cgaattgact agtacataac ttacggtaaa
    10861 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
    10921 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
    10981 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
    11041 caa
//
