LOCUS       sequence-243816-       10211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Singh A, Sweeney MF, Yu M, Burger A, Greninger P, Benes C, Haber DA,
            Settleman J
  TITLE     TAK1 Inhibition Promotes Apoptosis in KRAS-Dependent Colon Cancers.
  JOURNAL   Cell. 2012 Feb 17;148(4):639-50.
  PUBMED    22341439
REFERENCE   2  (bases 1 to 10211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2012-02-17"; volume: "148(4)"; pages: "639-50"
COMMENT     Sequence Label: pLenti-PGK-ER-KRAS(G12V)
FEATURES             Location/Qualifiers
     source          1..10211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     97..114
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    231..252
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(267..272,273..290,291..297)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    305..321
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     310..332
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     329..345
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     329..345
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     364..384
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        366..384
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        412..638
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             639..819
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    866..991
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1484..1717
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1902..1946
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2213..2330
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2376..2886
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2805..2823
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    2941..2965
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3961..3990,3991..3993,3994..4524)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="TEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKD
                     SEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIR
                     KHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(4526..4550)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4581..5169
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4634..4654)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5052..5063)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5225..5724
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5260..5283)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5657..5676
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5742..6767
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5742..6767
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             7141..7374
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7446..7567
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7483..7502)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7537..7556
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7607..7742
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7762..7781)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7763..7781)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7791..7808)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7791..7807)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7800..7822)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7949..8404
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8036..8055)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8246..8267
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8430..8534
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8535..8603,8604..9395)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8753..8772)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9566..10154
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10055..10074
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
       61 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      121 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
      181 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
      241 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
      301 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
      361 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
      421 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
      481 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
      541 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
      601 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
      661 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      721 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      781 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
      841 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
      901 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
      961 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     1021 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     1081 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     1141 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1201 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1261 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1321 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1381 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1441 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1501 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1561 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1621 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1681 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1741 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1801 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1861 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     1921 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     1981 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     2041 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     2101 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     2161 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
     2221 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2281 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg
     2341 agactagcct cgagaagctt gatatcgaat tccacggggt tggggttgcg ccttttccaa
     2401 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
     2461 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
     2521 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
     2581 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
     2641 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
     2701 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
     2761 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
     2821 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
     2881 ccccaggggg atccaccggt tcgtcgacta gtccagtgtg gtggaattct gcagatatca
     2941 acaagtttgt acaaaaaagc aggcttcacc atgccacgaa atgaaatggg tgcttcagga
     3001 gacatgaggg ctgccaacct ttggccaagc cctcttgtga ttaagcacac taagaagaat
     3061 agccctgcct tgtccttgac agctgaccag atggtcagtg ccttgttgga tgctgaaccg
     3121 cccatgatct attctgaata tgatccttct agacccttca gtgaagcctc aatgatgggc
     3181 ttattgacca acctagcaga tagggagctg gttcatatga tcaactgggc aaagagagtg
     3241 ccaggctttg gggacttgaa tctccatgat caggtccacc ttctcgagtg tgcctggctg
     3301 gagattctga tgattggtct cgtctggcgc tccatggaac acccggggaa gctcctgttt
     3361 gctcctaact tgctcctgga caggaatcaa ggtaaatgtg tggaaggcat ggtggagatc
     3421 tttgacatgt tgcttgctac gtcaagtcgg ttccgcatga tgaacctgca gggtgaagag
     3481 tttgtgtgcc tcaaatccat cattttgctt aattccggag tgtacacgtt tctgtccagc
     3541 accttgaagt ctctggaaga gaaggaccac atccaccgtg tcctggacaa gatcacagac
     3601 actttgatcc acctgatggc caaagctggc ctgactctgc agcagcagca tcgccgccta
     3661 gctcagctcc ttctcattct ttcccatatc cggcatatga gtaacaaacg catggagcat
     3721 ctctacaaca tgaaatgcaa gaacgtggta cccctctatg acctgctcct ggagatgttg
     3781 gatgcccacc gccttcatgc cccagccagt cgcatgggag tgcccccaga ggagcccagc
     3841 cagacccagc tggccaccac cagctccact tcagcacatt ccttacaaac ctactacata
     3901 cccccggaag cagagggctt ccccaacacg atccaagctt cgaattctgc agtcgacgaa
     3961 actgaatata aacttgtggt agttggagct gttggcgtag gcaagagtgc cttgacgata
     4021 cagctaattc agaatcattt tgtggacgaa tatgatccaa caatagagga ttcctacagg
     4081 aagcaagtag taattgatgg agaaacctgt ctcttggata ttctcgacac agcaggtcaa
     4141 gaggagtaca gtgcaatgag ggaccagtac atgaggactg gggagggctt tctttgtgta
     4201 tttgccataa ataatactaa atcatttgaa gatattcacc attatagaga acaaattaaa
     4261 agagttaagg actctgaaga tgtacctatg gtcctagtag gaaataaatg tgatttgcct
     4321 tctagaacag tagacacaaa acaggctcag gacttagcaa gaagttatgg aattcctttt
     4381 attgaaacat cagcaaagac aagacagggt gttgatgatg ccttctatac attagttcga
     4441 gaaattcgaa aacataaaga aaagatgagc aaagacggta aaaagaagaa aaagaagtca
     4501 aagacaaagt gtgtaattat gtaagaccca gctttcttgt acaaagtggt tgatatccag
     4561 cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     4621 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4681 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4741 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4801 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4861 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4921 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     4981 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5041 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5101 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5161 cctccccgcc tggaattctg cagatatccg gactagtgat ctctcgaggt taacgaattc
     5221 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     5281 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     5341 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     5401 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     5461 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     5521 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     5581 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     5641 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     5701 tcttcctcat ctccgggcct ttcgacctgc atcccgccac catgaaaaag cctgaactca
     5761 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     5821 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     5881 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     5941 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     6001 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     6061 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     6121 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     6181 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     6241 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     6301 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     6361 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     6421 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     6481 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     6541 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     6601 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     6661 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     6721 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagagt agatgccgac
     6781 cgaacaagag ctgatttcga gaacgcctca gccagcaact cgcgcgagcc tagcaaggca
     6841 aatgcgagag aacggcctta cgcttggtgg cacagttctc gtccacagtt cgctaagctc
     6901 gctcggctgg gtcgcgggag ggccggtcgc agtgattcag gcccttctgg attgtgttgg
     6961 tccccagggc acgattgtca tgcccacgca ctcgggtgat ctgactgatc ccgcagattg
     7021 gagatcgccg cccgtgcctg ccgattgggt gcagatccgt cgagggcccg gtacctttaa
     7081 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     7141 tggaagggct agctcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     7201 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7261 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7321 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     7381 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     7441 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7501 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7561 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     7621 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7681 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7741 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     7801 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7861 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7921 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     7981 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8041 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8101 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8161 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8221 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8281 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8341 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     8401 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8461 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8521 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8581 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8641 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8701 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8761 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8821 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8881 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8941 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     9001 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     9061 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     9121 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     9181 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9241 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9301 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9361 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9421 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9481 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9541 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9601 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9661 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9721 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9781 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9841 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9901 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9961 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    10021 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    10081 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    10141 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10201 cttttgctca c
//
