LOCUS       sequence-243842-        6141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene.
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pBabe-GFP-IKBalpha-wt
FEATURES             Location/Qualifiers
     source          1..6141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             23..492
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    557..756
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             957..1373
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1303..1325
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1341..1357
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2389..2409)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2394..2723
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2574..2709
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2732..2748)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2733..2749)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             2754..3473
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     LTR             3650..4119
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4249..4266)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4420..5008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4500..4519)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5168..5959,5960..6028))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5791..5810
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6029..6133)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca gcctgaatat gggccaaaca ggatatctgt ggtaagcagt
       61 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata
      121 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
      181 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
      241 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
      301 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
      361 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
      421 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
      481 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga
      541 cccaccaccg ggaggcaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt
      601 ctatgactga ttttatgcgc ctgcgtcggt actagttagc taactagctc tgtatctggc
      661 ggacccgtgg tggaactgac gagttctgaa cacccggccg caaccctggg agacgtccca
      721 gggactttgg gggccgtttt tgtggcccga cctgaggaag ggagtcgatg tggaatccga
      781 ccccgtcagg atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt
      841 ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca
      901 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc
      961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1021 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccggccgga
     1381 tctctcgagg ttagcttatg ttccaggcgg ccgagcgtcc ccaggagtgg gccatggagg
     1441 gcccccgcga cgggctgaag aaggagcggc tactggacga ccgccacgac agcggcctgg
     1501 actccatgaa agacgaggag tacgagcaga tggtcaagga gctgcaggag atccgcctcg
     1561 agccgcagga ggtgccgcgc ggctcggagc cctggaagca gcagctcacc gaggacgggg
     1621 actcgttcct gcacttggcc atcatccatg aagaaaaggc actgaccatg gaagtgatcc
     1681 gccaggtgaa gggagacctg gccttcctca acttccagaa caacctgcag cagactccac
     1741 tccacttggc tgtgatcacc aaccagccag aaattgctga ggcacttctg ggagctggct
     1801 gtgatcctga gctccgagac tttcgaggaa atacccccct acaccttgcc tgtgagcagg
     1861 gctgcctggc cagcgtggga gtcctgactc agtcctgcac caccccgcac ctccactcca
     1921 tcctgaaggc taccaactac aatggccaca cgtgtctaca cttagcctct atccatggct
     1981 acctgggcat cgtggagctt ttggtgtcct tgggtgctga tgtcaatgct caggagccct
     2041 gtaatggccg gactgccctt cacctcgcag tggacctgca aaatcctgac ctggtgtcac
     2101 tcctgttgaa gtgtggggct gatgtcaaca gagttaccta ccagggctat tctccctacc
     2161 agctcacctg gggccgccca agcacccgga tacagcagca gctgggccag ctgacactag
     2221 aaaaccttca gatgctgcca gagagtgagg atgaggagag ctatgacaca gagtcagagt
     2281 tcacggagtt cacagaggac gagctgccct atgatgactg tgtgtttgga ggccagcgtc
     2341 tgacgttatg aggatcaacg aattcgccag cacagtggtc gaccctgtgg aatgtgtgtc
     2401 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     2461 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     2521 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     2581 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     2641 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     2701 ttggaggcct aggcttttgc aaaaagctta tcgataccgt cgacctcgag accatggtga
     2761 gcaagcagat cctgaagaac accggcctgc aggagatcat gagcttcaag gtgaacctgg
     2821 agggcgtggt gaacaaccac gtgttcacca tggagggctg cggcaagggc aacatcctgt
     2881 tcggcaacca gctggtgcag atccgcgtga ccaagggcgc ccccctgccc ttcgccttcg
     2941 acatcctgag ccccgccttc cagtacggca accgcacctt caccaagtac cccgaggaca
     3001 tcagcgactt cttcatccag agcttccccg ccggcttcgt gtacgagcgc accctgcgct
     3061 acgaggacgg cggcctggtg gagatccgca gcgacatcaa cctgatcgag gagatgttcg
     3121 tgtaccgcgt ggagtacaag ggccgcaact tccccaacga cggccccgtg atgaagaaga
     3181 ccatcaccgg cctgcagccc agcttcgagg tggtgtacat gaacgacggc gtgctggtgg
     3241 gccaggtgat cctggtgtac cgcctgaaca gcggcaagtt ctacagctgc cacatgcgca
     3301 ccctgatgaa gagcaagggc gtggtgaagg acttccccga gtaccacttc atccagcacc
     3361 gcctggagaa gacctacgtg gaggacggcg gcttcgtgga gcagcacgag accgccatcg
     3421 cccagctgac cagcctgggc aagcccctgg gcagcctgca cgagtgggtg taatagggta
     3481 ccttcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     3541 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca
     3601 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg
     3661 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga
     3721 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     3781 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag
     3841 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     3901 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     3961 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat
     4021 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
     4081 cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt
     4141 aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg
     4201 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4261 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4321 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4381 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4441 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4501 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4561 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4621 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4681 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4741 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4801 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4861 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4921 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4981 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5041 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5101 cttcacctag atccttttgc ggccgcaaat caatctaaag tatatatgag taaacttggt
     5161 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5221 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5281 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5341 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5401 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5461 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5521 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5581 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5641 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5701 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5761 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5821 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5881 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5941 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6001 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6061 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6121 taggggttcc gcgcacattt c
//
