LOCUS       sequence-243850-        9441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tuning expression of PD-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9441)
  AUTHORS   Michaels YS, Barnkob MB, Barbosa H, Baeumler TA, Thompson MK, Andre 
            V, Colin-York H, Fritzsche M, Gileadi U, Sheppard HM, Knapp DJHF, 
            Milne TA, Cerundolo V, Fulga TA
  TITLE     Precise tuning of gene expression levels in mammalian cells.
  JOURNAL   Nat Commun. 2019 Feb 18;10(1):818. doi: 10.1038/s41467-019-08777-y.
  PUBMED    30778069
REFERENCE   2  (bases 1 to 9441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-02-18"; volume: "10(1):818. doi"; pages: " 
            10.1038/s41467-019-08777-y"
COMMENT     Sequence Label: PD-1-miSFIT-1x
FEATURES             Location/Qualifiers
     source          1..9441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1751..2206)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1888..1909)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2100..2119
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2333..2355
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2347..2364
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2348..2364
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2374..2393
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2374..2392
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2413..2548
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2588..2709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             2781..3014
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3101..3689
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3207..3218
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3616..3636
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     promoter        complement(4693..5203)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4756..4774)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        5231..5269
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    6340..6474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6611..6728
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6995..7039)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7224..7457
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7950..8075
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8122..8302
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8317..8520)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        8521..8900
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(9001..9021)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9001..9019)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9040..9056)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9040..9056)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9053..9075)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9064..9080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9088..9094,9095..9112,9113..9118))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9133..9154
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9271..9288)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     1801 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     1861 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     1921 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     1981 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     2041 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     2101 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     2161 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     2221 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     2281 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     2341 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     2401 ggtacgtccc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     2461 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     2521 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggata gctagagcca
     2581 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     2641 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     2701 aaacaagttc ctctcactct ctgatattca tttctttgca agttataaat actgaataat
     2761 aagatgacat gaactactac tgctagagat tttccacact gactaaaagg gtctgaggga
     2821 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     2881 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     2941 ctggtctaac cagagagacc cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg
     3001 aattagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt
     3061 gtaagtcatt ggtcttaaag gtaccgagct cgaattccag gcggggaggc ggcccaaagg
     3121 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
     3181 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
     3241 ccgcgcagaa tccaggtggc aacacaggcg agcagccatg gaaaggacgt cagcttcccc
     3301 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
     3361 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
     3421 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
     3481 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     3541 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     3601 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     3661 tcacaaattt tgtaatccag aggttgattg tcgactctag actcgactct agaggatccc
     3721 cctgttccac ctcttgaagg ctatgtaggc cacaaggccc acaaccacag cagccaggat
     3781 ggagcaatag acagggatga ggttgtcggt ggtgcctcgg gtcaccacgg gctgggagct
     3841 gcccatcact gtggtcacca cacctgccac cgtgctggct atgaggtctt gttctggagg
     3901 tgcctcaggc tcctgggtgc tgggggctgt gctgtccgag ccctctgggg gtgtggaccg
     3961 tgtaatccaa cggccaggga tctcctcgca ctcggcgtcg gcccagcgtg tgcactcgcg
     4021 gagctggcgc tcggtgtcct cgcacacggt gcagggcagg cacgggtcca cgtggttggc
     4081 ctcgtcggaa tacgtgccgt cggggcactc ctcgcacacg gtgttctgct tgtcctggca
     4141 ggagaacacg aggcccgagc ccgcctcgca cacgcggcac gcctcgcagc gcccagtcgt
     4201 ctcatcctgg tagtagccgt aggcgcagcg gcacacggcg tcgtcggcct ccacgcacgg
     4261 cgccgacatg ctctggagcc ccacgcactc ggtgcacggc ttgcacggct cggtcgcgct
     4321 caccacgtcg gagaacgtca cgctgtccag gcagggctca cacacggtct ggttggctcc
     4381 acaaggctgg gccacaccct cgcccaggtt gcaggctttg cagcactcac cgctgtgtgt
     4441 gtacaggcct gtggggcatg cctccttggc acctccaagg gacaccccca gaagcagcaa
     4501 cagcagcagg cgcggcccgt ccatggcgcg gccggtggca cctgccccca tcgcccgcct
     4561 cccgcggcag cgctcgactt ccagctcggt ccgctttgcg gactgatggg gctgcgctgc
     4621 gctgcgctcc agcgcccccc ctgcccgccg gagctggccg cggcccgaat tcctgcagcc
     4681 cgggggatcc ccctggggag agaggtcggt gattcggtca acgagggagc cgactgccga
     4741 cgtgcgctcc ggaggcttgc agaatgcgga acaccgcgcg ggcaggaaca gggcccacac
     4801 taccgcccca caccccgcct cccgcaccgc cccttcccgg ccgctgctct cggcgcgccc
     4861 tgctgagcag ccgctattgg ccacagccca tcgcggtcgg cgcgctgcca ttgctccctg
     4921 gcgctgtccg tctgcgaggg tactagtgag acgtgcggct tccgtttgtc acgtccggca
     4981 cgccgcgaac cgcaaggaac cttcccgact taggggcgga gcaggaagcg tcgccggggg
     5041 gcccacaagg gtagcggcga agatccgggt gacgctgcga acggacgtga agaatgtgcg
     5101 agacccaggg tcggcgccgc tgcgtttccc ggaaccacgc ccagagcagc cgcgtccctg
     5161 cgcaaaccca gggctgcctt ggaaaaggcg caaccccaac cccgtggaat tcgatatcaa
     5221 gcttctcgag ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
     5281 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggag
     5341 ctcctgcagg tgccgccatg cagatcccac aggcgccctg gccagtcgtc tgggcggtgc
     5401 tacaactggg ctggcggcca ggatggttct tagactcccc agacaggccc tggaaccccc
     5461 ccaccttctc cccagccctg ctcgtggtga ccgaagggga caacgccacc ttcacctgca
     5521 gcttctccaa cacatcggag agcttcgtgc taaactggta ccgcatgagc cccagcaacc
     5581 agacggacaa gctggccgcc ttccccgagg accgcagcca gcccggccag gactgccgct
     5641 tccgtgtcac acaactgccc aacgggcgtg acttccacat gagcgtggtc agggcccggc
     5701 gcaatgacag cggcacctac ctctgtgggg ccatctccct ggcccccaag gcgcagatca
     5761 aagagagcct gcgggcagag ctcagggtga cagagagaag ggcagaagtg cccacagccc
     5821 accccagccc ctcacccagg ccagccggcc agttccaaac cctggtggtt ggtgtcgtgg
     5881 gcggcctgct gggcagcctg gtgctgctag tctgggtcct ggccgtcatc tgctcccggg
     5941 ccgcacgagg gacaatagga gccaggcgca ccggccagcc cctgaaggag gacccctcag
     6001 ccgtgcctgt gttctctgtg gactatgggg agctggattt ccagtggcga gagaagaccc
     6061 cggagccccc cgtgccctgt gtccctgagc agacggagta tgccaccatt gtctttccta
     6121 gcggaatggg cacctcatcc cccgcccgca ggggctcagc cgacggccct cggagtgccc
     6181 agccactgag gcctgaggat ggacactgct cttggcccct ctgaggggta gcacgtgagt
     6241 gctagcctac ctgcactgta agcactttga ccggtactag cttagtcatg caccgggctg
     6301 caggaattcg atgacaggcg cagcttccga gggatttgag atccagacat gataagatac
     6361 attgatgagt ttggacaaac caaaactaga atgcagtgaa aaaaatgcct tatttgtgaa
     6421 atttgtgatg ctattgcctt atttgtaacc attataagct gcaataaaca agttaacaac
     6481 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     6541 aagtaaaacc tctacaaatg tggtatggct gattatgatc agatctctcg aggctagtct
     6601 cgtgatcgat aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt
     6661 ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc
     6721 ttttaaaagt taaccgatac cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt
     6781 ctctctctcc accttcttct tctattcctt cgggcctgtc gggtcccctc ggggttggga
     6841 ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta
     6901 cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat
     6961 accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca
     7021 ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta
     7081 agcttgtgta attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct
     7141 gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt
     7201 ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca
     7261 ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg
     7321 cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg
     7381 cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc
     7441 caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg
     7501 ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta tataattcac
     7561 ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc cgcttgctgt
     7621 gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct
     7681 tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta
     7741 agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct
     7801 tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct gcttgcccat
     7861 actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat
     7921 cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag
     7981 cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg
     8041 cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc
     8101 aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     8161 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     8221 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     8281 tctggtctaa ccagagagac cccggttcac taaacgagct ctgcttatat agacctccca
     8341 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
     8401 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     8461 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
     8521 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     8581 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     8641 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     8701 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     8761 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     8821 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     8881 actagtcaat aatcaatgtc aacatggcgg taatgttgga catgagccaa tataaatgta
     8941 catattatga tatggataca acgtatgcaa tggccaagct tgcagctcca gcttttgttc
     9001 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     9061 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     9121 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     9181 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     9241 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9301 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9361 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9421 aaaggccgcg ttgctggcgt t
//
