LOCUS       sequence-243856-        7547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PP7 coat protein fused to mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Coulon A, Ferguson ML, de Turris V, Palangat M, Chow CC, Larson DR
  TITLE     Kinetic competition during the transcription cycle results in 
            stochastic RNA processing.
  JOURNAL   Elife. 2014 Oct 1;3. doi: 10.7554/eLife.03939.
  PUBMED    25271374
REFERENCE   2  (bases 1 to 7547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3. doi"; pages: " 10.7554/eLife.03939"
COMMENT     Sequence Label: PP7_mCherry
FEATURES             Location/Qualifiers
     source          1..7547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        128..507
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        509..707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     662..682
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             725..905
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    952..1077
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1574..1807
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1992..2036
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2334..2451
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2519..2539
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2946..2970
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3412..3436)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3438..4145
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPYDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3580..3598)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3848..3867
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(4147..4167)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     4235..4251
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4326..4914
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4379..4399)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4797..4808)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4917..4933)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             5443..5623
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5685..6273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5765..5784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6444..7235,7236..7304))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7067..7086
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7305..7409)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7484..7503)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
       61 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
      121 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      181 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      241 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      301 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      361 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      421 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      481 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      541 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      601 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      661 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg
      721 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      781 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      841 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      901 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      961 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     1021 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     1081 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     1141 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     1201 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     1261 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     1321 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     1381 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
     1441 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1501 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1561 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1621 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1681 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1741 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1801 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1861 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1921 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1981 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2041 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2101 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2161 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2221 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2281 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2341 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2401 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2461 tacagggaca gcagagatcc agtttggtta gtaccgggcc cgctctagat tgaattatca
     2521 actttgtata gaaaagttgg cgactagtgg cctccgcgcc gggttttggc gcctcccgcg
     2581 ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct
     2641 gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa
     2701 ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt
     2761 ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag
     2821 ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag
     2881 ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt
     2941 cacttacaac tttgtacaaa aaagttggca ccatgtcgcg gaggcggcac agctacgaga
     3001 acgatggtgg acaacctcac aaaaggagga agacgtctat gctagcctcc aaaaccatcg
     3061 ttctttcggt cggcgaggct actcgcactc tgactgagat ccagtccacc gcagaccgtc
     3121 agatcttcga agagaaggtc gggcctctgg tgggtcggct gcgcctcacg gcttcgctcc
     3181 gtcaaaacgg agccaagacc gcgtatcgcg tcaacctaaa actggatcag gcggacgtcg
     3241 ttgattccgg acttccgaaa gtgcgctaca ctcaggtatg gtcgcacgac gtgacaatcg
     3301 ttgcgaatag caccgaggcc tcgcgcaaat cgttgtacga tttgaccaag tccctcgtcg
     3361 cgacctcgca ggtcgaagat cttgtcgtca accttgtgcc gctgggccgt tcttcaactt
     3421 tcttgtacaa agtgggcgtg agcaagggcg aggaggataa catggccatc atcaaggagt
     3481 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     3541 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     3601 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     3661 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     3721 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     3781 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     3841 acgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     3901 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggag
     3961 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     4021 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     4081 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     4141 agtaatcaac tttattatac atagttgata attcaaggga tctagggtgt ggaaagtccc
     4201 caggctcccc agcaggcaga agtatgcaaa gcatcgaggt cgacggtatc gataagctcg
     4261 cttcacgaga tcatgtttaa gggttccggt tccactaggt acaattcgat atcaagctta
     4321 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4381 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4441 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4501 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4561 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4621 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4681 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     4741 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4801 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4861 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     4921 accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     4981 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     5041 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5101 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     5161 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     5221 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     5281 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     5341 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     5401 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     5461 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5521 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5581 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg agcaaaaggc
     5641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5881 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5941 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6001 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6061 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6121 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6181 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6241 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6301 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6361 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6421 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6481 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6541 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6601 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6661 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6721 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6781 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6841 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6901 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6961 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7021 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7081 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7141 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7201 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7261 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7321 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7381 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     7441 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     7501 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtc
//
