LOCUS       sequence-243867-       17069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses multimeric human VWF-ECFP fusion protein. The cyan 
            fluorescence protein coding region is inserted between the VWF-D'D3 
            and A1 domains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17069)
  AUTHORS   Zhang C, Kelkar A, Neelamegham S
  TITLE     von Willebrand factor self-association is regulated by the 
            shear-dependent unfolding of the A2 domain.
  JOURNAL   Blood Adv. 2019 Apr 9;3(7):957-968. doi: 
            10.1182/bloodadvances.2018030122.
  PUBMED    30936056
REFERENCE   2  (bases 1 to 17069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/bloodadvances"; journalName: "Blood Adv"; date: "2019-04-9-
            9"; volume: "3"; issue: "7"; pages: "957-968"
COMMENT     Sequence Label: pCS-CG-WT-Cer-VWF
FEATURES             Location/Qualifiers
     source          1..17069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2229..2532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2533..2736
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4394..4405
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             join(6615..6617,6618..6620,6621..7133)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(6660..6681)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6921..6940)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7268..7289
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             12086..12106
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             12107..12124
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     LTR             12637..12817
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12843..12860)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12849..13073
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      13119..13547
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13206..13225)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13416..13437
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13556..13576)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13561..13890
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13741..13876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13803..13822
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        13938..13985
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             14004..14378
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    14508..14629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14545..14564)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14599..14618
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(14678..14694)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14678..14694)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14691..14713)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14702..14718
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14726..14732,14733..14750,14751..14756))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14771..14792
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14909..14926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15080..15668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15160..15179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15839..16630,16631..16699))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16462..16481
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16700..16804)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(16879..16898)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcccccggg
     2161 ctgcaggaat tcgattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     2221 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2281 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2341 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2401 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2461 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2521 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2581 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2641 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     2701 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc
     2761 tagcaggcgc gccagtcgac tcccgggatc ccaattcccg cagccctcat ttgcagggga
     2821 agatgattcc tgccagattt gccggggtgc tgcttgctct ggccctcatt ttgccaggga
     2881 ccctttgtgc agaaggaact cgcggcaggt catccacggc ccgatgcagc cttttcggaa
     2941 gtgacttcgt caacaccttt gatgggagca tgtacagctt tgcgggatac tgcagttacc
     3001 tcctggcagg gggctgccag aaacgctcct tctcgattat tggggacttc cagaatggca
     3061 agagagtgag cctctccgtg tatcttgggg aattttttga catccatttg tttgtcaatg
     3121 gtaccgtgac acagggggac caaagagtct ccatgcccta tgcctccaaa gggctgtatc
     3181 tagaaactga ggctgggtac tacaagctgt ccggtgaggc ctatggcttt gtggccagga
     3241 tcgatggcag cggcaacttt caagtcctgc tgtcagacag atacttcaac aagacctgcg
     3301 ggctgtgtgg caactttaac atctttgctg aagatgactt tatgacccaa gaagggacct
     3361 tgacctcgga cccttatgac tttgccaact catgggctct gagcagtgga gaacagtggt
     3421 gtgaacgggc atctcctccc agcagctcat gcaacatctc ctctggggaa atgcagaagg
     3481 gcctgtggga gcagtgccag cttctgaaga gcacctcggt gtttgcccgc tgccaccctc
     3541 tggtggaccc cgagcctttt gtggccctgt gtgagaagac tttgtgtgag tgtgctgggg
     3601 ggctggagtg cgcctgccct gccctcctgg agtacgcccg gacctgtgcc caggagggaa
     3661 tggtgctgta cggctggacc gaccacagcg cgtgcagccc agtgtgccct gctggtatgg
     3721 agtataggca gtgtgtgtcc ccttgcgcca ggacctgcca gagcctgcac atcaatgaaa
     3781 tgtgtcagga gcgatgcgtg gatggctgca gctgccctga gggacagctc ctggatgaag
     3841 gcctctgcgt ggagagcacc gagtgtccct gcgtgcattc cggaaagcgc taccctcccg
     3901 gcacctccct ctctcgagac tgcaacacct gcatttgccg aaacagccag tggatctgca
     3961 gcaatgaaga atgtccaggg gagtgccttg tcacaggtca atcacacttc aagagctttg
     4021 acaacagata cttcaccttc agtgggatct gccagtacct gctggcccgg gattgccagg
     4081 accactcctt ctccattgtc attgagactg tccagtgtgc tgatgaccgc gacgctgtgt
     4141 gcacccgctc cgtcaccgtc cggctgcctg gcctgcacaa cagccttgtg aaactgaagc
     4201 atggggcagg agttgccatg gatggccagg acgtccagct ccccctcctg aaaggtgacc
     4261 tccgcatcca gcatacagtg acggcctccg tgcgcctcag ctacggggag gacctgcaga
     4321 tggactggga tggccgcggg aggctgctgg tgaagctgtc ccccgtctat gccgggaaga
     4381 cctgcggcct gtgtgggaat tacaatggca accagggcga cgacttcctt accccctctg
     4441 ggctggcgga gccccgggtg gaggacttcg ggaacgcctg gaagctgcac ggggactgcc
     4501 aggacctgca gaagcagcac agcgatccct gcgccctcaa cccgcgcatg accaggttct
     4561 ccgaggaggc gtgcgcggtc ctgacgtccc ccacattcga ggcctgccat cgtgccgtca
     4621 gcccgctgcc ctacctgcgg aactgccgct acgacgtgtg ctcctgctcg gacggccgcg
     4681 agtgcctgtg cggcgccctg gccagctatg ccgcggcctg cgcggggaga ggcgtgcgcg
     4741 tcgcgtggcg cgagccaggc cgctgtgagc tgaactgccc gaaaggccag gtgtacctgc
     4801 agtgcgggac cccctgcaac ctgacctgcc gctctctctc ttacccggat gaggaatgca
     4861 atgaggcctg cctggagggc tgcttctgcc ccccagggct ctacatggat gagagggggg
     4921 actgcgtgcc caaggcccag tgcccctgtt actatgacgg tgagatcttc cagccagaag
     4981 acatcttctc agaccatcac accatgtgct actgtgagga tggcttcatg cactgtacca
     5041 tgagtggagt ccccggaagc ttgctgcctg acgctgtcct cagcagtccc ctgtctcatc
     5101 gcagcaaaag gagcctatcc tgtcggcccc ccatggtcaa gctggtgtgt cccgctgaca
     5161 acctgcgggc tgaagggctc gagtgtacca aaacgtgcca gaactatgac ctggagtgca
     5221 tgagcatggg ctgtgtctct ggctgcctct gccccccggg catggtccgg catgagaaca
     5281 gatgtgtggc cctggaaagg tgtccctgct tccatcaggg caaggagtat gcccctggag
     5341 aaacagtgaa gattggctgc aacacttgtg tctgtcggga ccggaagtgg aactgcacag
     5401 accatgtgtg tgatgccacg tgctccacga tcggcatggc ccactacctc accttcgacg
     5461 ggctcaaata cctgttcccc ggggagtgcc agtacgttct ggtgcaggat tactgcggca
     5521 gtaaccctgg gacctttcgg atcctagtgg ggaataaggg atgcagccac ccctcagtga
     5581 aatgcaagaa acgggtcacc atcctggtgg agggaggaga gattgagctg tttgacgggg
     5641 aggtgaatgt gaagaggccc atgaaggatg agactcactt tgaggtggtg gagtctggcc
     5701 ggtacatcat tctgctgctg ggcaaagccc tctccgtggt ctgggaccgc cacctgagca
     5761 tctccgtggt cctgaagcag acataccagg agaaagtgtg tggcctgtgt gggaattttg
     5821 atggcatcca gaacaatgac ctcaccagca gcaacctcca agtggaggaa gaccctgtgg
     5881 actttgggaa ctcctggaaa gtgagctcgc agtgtgctga caccagaaaa gtgcctctgg
     5941 actcatcccc tgccacctgc cataacaaca tcatgaagca gacgatggtg gattcctcct
     6001 gtagaatcct taccagtgac gtcttccagg actgcaacaa gctggtggac cccgagccat
     6061 atctggatgt ctgcatttac gacacctgct cctgtgagtc cattggggac tgcgcctgct
     6121 tctgcgacac cattgctgcc tatgcccacg tgtgtgccca gcatggcaag gtggtgacct
     6181 ggaggacggc cacattgtgc ccccagagct gcgaggagag gaatctccgg gagaacgggt
     6241 atgagtgtga gtggcgctat aacagctgtg cacctgcctg tcaagtcacg tgtcagcacc
     6301 ctgagccact ggcctgccct gtgcagtgtg tggagggctg ccatgcccac tgccctccag
     6361 ggaaaatcct ggatgagctt ttgcagacct gcgttgaccc tgaagactgt ccagtgtgtg
     6421 aggtggctgg ccggcgtttt gcctcaggaa agaaagtcac cttgaatccc agtgaccctg
     6481 agcactgcca gatttgccac tgtgatgttg tcaacctcac ctgtgaagcc tgccaggagc
     6541 cgggaggcct ggtggtgcct cccacagatg ccccggtgag ccccaccact ctgtatgtgg
     6601 aggacatctt cgaaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     6661 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     6721 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     6781 tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc
     6841 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     6901 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     6961 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     7021 acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg
     7081 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca
     7141 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     7201 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     7261 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     7321 agttcgaacc gccattgcac gatttctact gcagcaggct actggacctg gtcttcctgc
     7381 tggatggctc ctccaggctg tccgaggctg agtttgaagt gctgaaggcc tttgtggtgg
     7441 acatgatgga gcggctgcgc atctcccaga agtgggtccg cgtggccgtg gtggagtacc
     7501 acgacggctc ccacgcctac atcgggctca aggaccggaa gcgaccgtca gagctgcggc
     7561 gcattgccag ccaggtgaag tatgcgggca gccaggtggc ctccaccagc gaggtcttga
     7621 aatacacact gttccaaatc ttcagcaaga tcgaccgccc tgaagcctcc cgcatcgccc
     7681 tgctcctgat ggccagccag gagccccaac ggatgtcccg gaactttgtc cgctacgtcc
     7741 agggcctgaa gaagaagaag gtcattgtga tcccggtggg cattgggccc catgccaacc
     7801 tcaagcagat ccgcctcatc gagaagcagg cccctgagaa caaggccttc gtgctgagca
     7861 gtgtggatga gctggagcag caaagggacg agatcgttag ctacctctgt gaccttgccc
     7921 ctgaagcccc tcctcctact ctgccccccc acatggcaca agttaacgta ggtccggggc
     7981 tcttgggggt ttcgaccctg gggcccaaga ggaactccat ggttctggat gtggcgttcg
     8041 tcctggaagg atcggacaaa attggtgaag ccgacttcaa caggagcaag gagttcatgg
     8101 aggaggtgat tcagcggatg gatgtgggcc aggacagcat ccacgtcacg gtgctgcagt
     8161 actcctacat ggtgaccgtg gagtacccct tcagcgaggc acagtccaaa ggggacatcc
     8221 tgcagcgggt gcgagagatc cgctaccagg gcggcaacag gaccaacact gggctggccc
     8281 tgcggtacct ctctgaccac agcttcttgg tcagccaggg tgaccgggag caggcgccca
     8341 acctggtcta catggtcacc ggaaatcctg cctctgatga gatcaagagg ctgcctggag
     8401 acatccaggt ggtgcccatt ggagtgggcc ctaatgccaa cgtgcaggag ctggagagga
     8461 ttggctggcc caatgcccct atcctcatcc aggactttga gacgctcccc cgagaggctc
     8521 ctgacctggt gctgcagagt tgttgtaccg gtgaggggct gcagatcccc accctctccc
     8581 ctgcacctga ctgcagccag cccctggacg tgatccttct cctggatggc tcctccagtt
     8641 tcccagcttc ttattttgat gaaatgaaga gtttcgccaa ggctttcatt tcaaaagcca
     8701 atatagggcc tcgtctcact caggtgtcag tgctgcagta tggaagcatc accaccattg
     8761 acgtgccatg gaacgtggtc ccggagaaag cccatttgct gagccttgtg gacgtcatgc
     8821 agcgggaggg aggccccagc caaatcgggg atgccttggg ctttgctgtg cgatacttga
     8881 cttcagaaat gcatggtgcc aggccgggag cctcaaaggc ggtggtcatc ctggtcacgg
     8941 acgtctctgt ggattcagtg gatgcagcag ctgatgccgc caggtccaac agagtgacag
     9001 tgttccctat tggaattgga gatcgctacg atgcagccca gctacggatc ttggcaggcc
     9061 cagcaggcga ctccaacgtg gtgaagctcc agcgaatcga agacctccct accatggtca
     9121 ccttgggcaa ttccttcctc cacaaactgt gctctggatt tgttaggatt tgcatggatg
     9181 aggatgggaa tgagaagagg cccggggacg tctggacctt gccagaccag tgccacaccg
     9241 tgacttgcca gccagatggc cagaccttgc tgaagagtca tcgggtcaac tgtgaccggg
     9301 ggctgaggcc ttcgtgccct aacagccagt cccctgttaa agtggaagag acctgtggct
     9361 gccgctggac ctgcccctgc gtgtgcacag gcagctccac tcggcacatc gtgacctttg
     9421 atgggcagaa tttcaagctg actggcagct gttcttatgt cctatttcaa aacaaggagc
     9481 aggacctgga ggtgattctc cataatggtg cctgcagccc tggagcaagg cagggctgca
     9541 tgaaatccat cgaggtgaag cacagtgccc tctccgtcga gctgcacagt gacatggagg
     9601 tgacggtgaa tgggagactg gtctctgttc cttacgtggg tgggaacatg gaagtcaacg
     9661 tttatggtgc catcatgcat gaggtcagat tcaatcacct tggtcacatc ttcacattca
     9721 ctccacaaaa caatgagttc caactgcagc tcagccccaa gacttttgct tcaaagacgt
     9781 atggtctgtg tgggatctgt gatgagaacg gagccaatga cttcatgctg agggatggca
     9841 cagtcaccac agactggaaa acacttgttc aggaatggac tgtgcagcgg ccagggcaga
     9901 cgtgccagcc catcctggag gagcagtgtc ttgtccccga cagctcccac tgccaggtcc
     9961 tcctcttacc actgtttgct gaatgccaca aggtcctggc tccagccaca ttctatgcca
    10021 tctgccagca ggacagttgc caccaggagc aagtgtgtga ggtgatcgcc tcttatgccc
    10081 acctctgtcg gaccaacggg gtctgcgttg actggaggac acctgatttc tgtgctatgt
    10141 catgcccacc atctctggtc tacaaccact gtgagcatgg ctgtccccgg cactgtgatg
    10201 gcaacgtgag ctcctgtggg gaccatccct ccgaaggctg tttctgccct ccagataaag
    10261 tcatgttgga aggcagctgt gtccctgaag aggcctgcac tcagtgcatt ggtgaggatg
    10321 gagtccagca ccagttcctg gaagcctggg tcccggacca ccagccctgt cagatctgca
    10381 catgcctcag cgggcggaag gtcaactgca caacgcagcc ctgccccacg gccaaagctc
    10441 ccacgtgtgg cctgtgtgaa gtagcccgcc tccgccagaa tgcagaccag tgctgccccg
    10501 agtatgagtg tgtgtgtgac ccagtgagct gtgacctgcc cccagtgcct cactgtgaac
    10561 gtggcctcca gcccacactg accaaccctg gcgagtgcag acccaacttc acctgcgcct
    10621 gcaggaagga ggagtgcaaa agagtgtccc caccctcctg ccccccgcac cgtttgccca
    10681 cccttcggaa gacccagtgc tgtgatgagt atgagtgtgc ctgcaactgt gtcaactcca
    10741 cagtgagctg tccccttggg tacttggcct caaccgccac caatgactgt ggctgtacca
    10801 caaccacctg ccttcccgac aaggtgtgtg tccaccgaag caccatctac cctgtgggcc
    10861 agttctggga ggagggctgc gatgtgtgca cctgcaccga catggaggat gccgtgatgg
    10921 gcctccgcgt ggcccagtgc tcccagaagc cctgtgagga cagctgtcgg tcgggcttca
    10981 cttacgttct gcatgaaggc gagtgctgtg gaaggtgcct gccatctgcc tgtgaggtgg
    11041 tgactggctc accgcggggg gactcccagt cttcctggaa gagtgtcggc tcccagtggg
    11101 cctccccgga gaacccctgc ctcatcaatg agtgtgtccg agtgaaggag gaggtcttta
    11161 tacaacaaag gaacgtctcc tgcccccagc tggaggtccc tgtctgcccc tcgggctttc
    11221 agctgagctg taagacctca gcgtgctgcc caagctgtcg ctgtgagcgc atggaggcct
    11281 gcatgctcaa tggcactgtc attgggcccg ggaagactgt gatgatcgat gtgtgcacga
    11341 cctgccgctg catggtgcag gtgggggtca tctctggatt caagctggag tgcaggaaga
    11401 ccacctgcaa cccctgcccc ctgggttaca aggaagaaaa taacacaggt gaatgttgtg
    11461 ggagatgttt gcctacggct tgcaccattc agctaagagg aggacagatc atgacactga
    11521 agcgtgatga gacgctccag gatggctgtg atactcactt ctgcaaggtc aatgagagag
    11581 gagagtactt ctgggagaag agggtcacag gctgcccacc ctttgatgaa cacaagtgtc
    11641 tggctgaggg aggtaaaatt atgaaaattc caggcacctg ctgtgacaca tgtgaggagc
    11701 ctgagtgcaa cgacatcact gccaggctgc agtatgtcaa ggtgggaagc tgtaagtctg
    11761 aagtagaggt ggatatccac tactgccagg gcaaatgtgc cagcaaagcc atgtactcca
    11821 ttgacatcaa cgatgtgcag gaccagtgct cctgctgctc tccgacacgg acggagccca
    11881 tgcaggtggc cctgcactgc accaatggct ctgttgtgta ccatgaggtt ctcaatgcca
    11941 tggagtgcaa atgctccccc aggaagtgca gcaagtgaga attgctagcg ccgccaccat
    12001 gattcctgcc agatttgccg gggtgctgct tgctctggcc ctcattttgc cagggaccct
    12061 ttgtaccggt ccgcgggtta acttcgaaaa cctgtacttc cagggccatc accatcacca
    12121 tcactgactc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
    12181 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
    12241 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
    12301 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
    12361 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
    12421 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
    12481 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
    12541 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
    12601 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
    12661 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
    12721 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    12781 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
    12841 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
    12901 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
    12961 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
    13021 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
    13081 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
    13141 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
    13201 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
    13261 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
    13321 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    13381 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    13441 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
    13501 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
    13561 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    13621 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
    13681 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
    13741 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    13801 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
    13861 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
    13921 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
    13981 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
    14041 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
    14101 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
    14161 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
    14221 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
    14281 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
    14341 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
    14401 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
    14461 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
    14521 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    14581 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
    14641 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    14701 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    14761 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    14821 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    14881 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    14941 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    15001 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    15061 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    15121 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    15181 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    15241 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    15301 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    15361 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    15421 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    15481 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    15541 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    15601 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    15661 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    15721 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    15781 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    15841 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    15901 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    15961 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    16021 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    16081 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    16141 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    16201 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    16261 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    16321 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    16381 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    16441 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    16501 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    16561 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    16621 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    16681 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    16741 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    16801 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
    16861 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
    16921 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
    16981 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
    17041 tcgcgatgta cgggccagat atacgcgtt
//
