LOCUS       sequence-243880-        5561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAGIC donor plasmid (Himar transposon with catP/sfGFP payload).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Ronda C, Chen SP, Cabral V, Yaung SJ, Wang HH
  TITLE     Metagenomic engineering of the mammalian gut microbiome in situ.
  JOURNAL   Nat Methods. 2019 Feb;16(2):167-170. doi: 10.1038/s41592-018-0301-y.
            Epub 2019 Jan 14.
  PUBMED    30643213
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0301-y"; journalName: "Nat Methods"; date: 
            "2019-02"; volume: "16"; issue: "2"; pages: "167-170"
COMMENT     Sequence Label: pGT-Ah7
FEATURES             Location/Qualifiers
     source          1..5561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             979..1692
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGFVTILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1752..1838
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1930..1957
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      3327..3872
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     3773..3792
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3989..4006
                     /label=L4440
                     /note="L4440 vector, forward primer"
     oriT            4142..4251
                     /note="incP origin of transfer"
     CDS             complement(4572..5381)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5295..5314
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(5382..5486)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(5554..5561,1..11)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tatcacgagg ccctttcgtc acgcgtcctc ggtacctaac aggttggatg ataagtcccc
       61 ggtcttcgta tgccgtcttc tgcttggcgc gccctcgagc aattgccgac cgaattttta
      121 tgtcgtaaag aggggctttg cagggggtgg actcagaaag atgagaatag atgactattg
      181 tagttgaaac acgcaagtgt tcaagaagtt attaagtcgg gagtgcagtc gaagtgggca
      241 agttgaaaaa ttcacaaaaa tgtggtataa tatctttgtt cattagagcg ataaacttga
      301 atttgagagg gaacttagat ggtatttgaa aaaattgata aaaatagttg gaacagaaaa
      361 gagtattttg accactactt tgcaagtgta ccttgtacat acagcatgac cgttaaagtg
      421 gatatcacac aaataaagga aaagggaatg aaactatatc ctgcaatgct ttattatatt
      481 gcaatgattg taaaccgcca ttcagagttt aggacggcaa tcaatcaaga tggtgaattg
      541 gggatatatg atgagatgat accaagctat acaatatttc acaatgatac tgaaacattt
      601 tccagccttt ggactgagtg taagtctgac tttaaatcat ttttagcaga ttatgaaagt
      661 gatacgcaac ggtatggaaa caatcataga atggaaggaa agccaaatgc tccggaaaac
      721 atttttaatg tatctatgat accgtggtca accttcgatg gctttaatct gaatttgcag
      781 aaaggatatg attatttgat tcctattttt actatgggga aatattataa agaagataac
      841 aaaattatac ttcctttggc aattcaagtt catcacgcag tatgtgacgg atttcacatt
      901 tgccgttttg taaacgaatt gcaggaattg ataaatagtt aaattatatt taaactcaat
      961 ttaaagtagg gagaatagat gcgtaaaggc gaagagctgt tcactggttt cgtcactatt
     1021 ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa
     1081 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg
     1141 gtaccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat
     1201 ccggaccaca tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag
     1261 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt
     1321 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc
     1381 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc
     1441 gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc
     1501 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg
     1561 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa
     1621 cgcgatcaca tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat
     1681 gaactgtaca aataaaaaca ataggccaca tgcaactgtc tagaatgcga gagtagggaa
     1741 ctgccagaca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1801 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     1861 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc
     1921 aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacct gcagggcgcg
     1981 ccaagcagaa gacggcatac gaagaccggg gacttatcat ccaacctgtt aggatccata
     2041 aggattcttt aaagagagat atagttatgt caaagactgt agaattttta gtaaatcaaa
     2101 ataaaaaaag aggtattaaa tagagtgtat tttaaaggag gagacttcat atggaaaaaa
     2161 aggaatttcg tgttttgata aaatactgtt ttctgaaggg aaaaaataca gtggaagcaa
     2221 aaacttggct tgataatgag tttccggact ctgccccagg gaaatcaaca ataattgatt
     2281 ggtatgcaaa attcaagcgt ggtgaaatga gcacggagga cggtgaacgc agtggacgcc
     2341 cgaaagaggt ggttaccgac gaaaacatca aaaaaatcca caaaatgatt ttgaatgacc
     2401 gtaaaatgaa gttgatcgag atagcagagg ccttaaagat atcaaaggaa cgtgttggtc
     2461 atatcattca tcaatatttg gatatgcgga agctctgtgc gaaatgggtg ccgcgcgagc
     2521 tcacatttga ccaaaaacaa cgacgtgttg atgattctaa gcggtgtttg cagctgttaa
     2581 ctcgtaatac acccgagttt ttccgtcgat atgtgacaat ggatgaaaca tggctccatc
     2641 actacactcc tgagtccaat cgacagtcgg ctgagtggac agcgaccggt gaaccgtctc
     2701 cgaagcgtgg aaagactcaa aagtccgctg gcaaagtaat ggcctctgtt ttttgggatg
     2761 cgcatggaat aatttttatc gattatcttg agaagggaaa aaccatcaac agtgactatt
     2821 atatggcgtt attggagcgt ttgaaggtcg aaatcgcggc aaaacggccc cacatgaaga
     2881 agaaaaaagt gttgttccac caagacaacg caccgtgcca caagtcattg agaacgatgg
     2941 caaaaattca tgaattgggc ttcgaattgc ttccccaccc gccgtattct ccagatctgg
     3001 cccccagcga ctttttcttg ttctcagacc tcaaaaggat gctcgcaggg aaaaaatttg
     3061 gctgcaatga agaggtgatc gccgaaactg aggcctattt tgaggcaaaa ccgaaggagt
     3121 actaccaaaa tggtatcaaa aaattggaag gtcgttataa tcgttgtatc gctcttgaag
     3181 ggaactatgt tgaataagcg gccgccaccg cggttgaggg gaattaacaa cttatatcgt
     3241 atggggctga cttcaggtgc tacatttgaa gagataaatt gcactgaaat ctagaaatat
     3301 tttatctgat taataagatg atcttcttga gatcgttttg gtctgcgcgt aatctcttgc
     3361 tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg agctaccaac
     3421 tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt cctttcagtt
     3481 tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac cagtggctgc
     3541 tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt taccggataa
     3601 ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc
     3661 ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga
     3721 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca gggggaaacg
     3781 cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt
     3841 gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc ctctcacttc
     3901 cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt aagccatttc
     3961 cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatat
     4021 ccagatcctc tacgccggac gcatcgtggc cggatcaatt ccgtgatagg tgggctgccc
     4081 ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta
     4141 gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
     4201 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
     4261 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
     4321 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
     4381 gaaaacggaa ttgatccccc tcgaattgac gcgtatccag caatagacat aagcggctat
     4441 ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc
     4501 atccgcttat tatcacttat tcaggcgtag caaccaggcg tttaagggca ccaataactg
     4561 ccttaaaaaa attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     4621 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     4681 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     4741 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     4801 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt
     4861 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     4921 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     4981 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     5041 gaatcaggat attcttctaa tacctggaat gctgtttttc cggggatcgc agtggtgagt
     5101 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     5161 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     5221 tgtttcagaa acaactctgg cgcatcgggc ttcccataca agcgatagat tgtcgcacct
     5281 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     5341 tttaatcgcg gcctcgacgt ttcccgttga atatggctca tactcttcct ttttcaatat
     5401 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5461 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     5521 gaaaccatta ttatcatgac attaacctat aaaaataggc g
//
