LOCUS       sequence-243887-        5904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV control with intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5904)
  TITLE     Oka Lab plasmids
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AV0.1-C346BE2.8-2
FEATURES             Location/Qualifiers
     source          1..5904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   3135..3275
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3135..3264
                     /label=AAV2 ITR
     rep_origin      3350..3805
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3437..3456)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3647..3668
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3822..3841)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3941..3963
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4001..4019)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4087..4191
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4192..4260,4261..5052)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4410..4429)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5223..5811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5712..5731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(5873..5904,1..98)
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gaggccgccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg
       61 cagagaggga gtggccaact ccatcactag gggttcctgc ggccgtggag tcgtgacgtg
      121 aattacgtca tagggttagg gaggtcctgt attagaggtc acgtgagtgt tttgcgacat
      181 tttgcgacac catgtggtca cgctgggtat ttaagcccga gtgagcacgc agggtctcca
      241 tacgcgccga cgcgcatgct cctctagact cgaggaattc tgggctgtgg cattttgagc
      301 agcagcatgc tgttccaaaa tgtcgtcgat cagcctcaag ttgcacaccc agtcttcatc
      361 tgggctcaca caggagcctt tcaagagagc ttcaatgaaa tctacctcat tgcagtcagg
      421 tgacgaaatc agatcattta gtgggggttg gggctggcgc aaaaagtcgg caggtggcag
      481 ctcaggggga atatccgttc tgtcgaacgg acctgggaac tggctggcag caacggcaga
      541 agcagcagca gcggtggcag cagcagccac atagcttggt ggctcgatgc cctgtatggg
      601 gctcagggga ctaaagctgg ccataccctg ctggaggaac ttggtggtgt ttgctacagg
      661 caccgggccc tgtaccgggc tctgcctgag gctctggctg cccagcaggc tgaagctggg
      721 gttgttggcc aggggcactt gtgttcccat cgcagcgggc acttgtgcct cccaatcaga
      781 tggcctctga aggcaggcct ggccagaagg tgagtgctgc tgaacgctat tatccacttg
      841 gctgaggggt gttttccccg aaactgctgt ggtcacagct gctgccgctg tgacccatgc
      901 agcattgttg aacgcagtgg gcattcttgg cacactaggc cgtctgagct ggtggggact
      961 caaggactgg gtgcccaggg agctgggaca gaacccaggc aggggcactt ctggtggggt
     1021 ggccttgggg ctctgcatat gctggcagac agagtcaagt ctgcccaggg gagtctggcc
     1081 tgagtgtgag aggatgggac actgggggct ggaggtgaaa attccttgcc gcttccccag
     1141 agttggtgag atcactccca tgccctcgca gctctggtgc ctggtgagtg ggatcattcc
     1201 tggactcaga ttgttctgaa gaagcccagt tctgggtggc atcaagtgct tgctagatgg
     1261 ggggcttgcc ttgatccggc tacacttgga ggtgacttgt tcttggacgg ctacatacag
     1321 aaagagagaa gtggggatga gttccaaagg catcctcgac ttcggctgtg gccaccggag
     1381 ggtagctcct ggcccaacac ggacttctca cctcccgccc ttggctctct actgagctcc
     1441 cccctgctcc ccaattcctc gccattcccc tcatttctct gccctcagcc tggactgcag
     1501 ttcttctggg aagctgcccc aactccctag gtctgtgctc accaagagca gatcacactg
     1561 gactgaaatg ccagctgatt tgtctcttca agaaaattgg aagctcctgg aggtcagggt
     1621 ccatgtctgc ttttacactc agtgctctgt atgcaggcct ggcactgccc accctttgac
     1681 aggtggtgca tattttgtag aaggaaggaa ggggccaggt ggggtgggct gggctggtgg
     1741 cgggagctag ctcagcctct tagattctct acccgatgga tgtgacctgg gacagcaagt
     1801 gagtgtggtg agtgagtgca gacggtgctt tgttcccctc ttgtctcata gcctagatgg
     1861 cctctgagcc cagatctggg gctcagacaa catttgttca actgaacggt aatgggtttc
     1921 ctttctgaag gctgaaatct gggagctgac attctggact ccctgagttc tgaagagcct
     1981 ggggatggag agacacggag cagaagatgg aaggtagagt cccaggtgcc taagatgggg
     2041 aatacatctc ccctcattgt catgagagtc cactctagct gatatctact gtggccaata
     2101 tctaccggta cttttttggg gtggacactg agtcatgcag cagtcttatg gtttacccaa
     2161 ggtcaggtag gggagacagt gcagtcagag cacaagccca gtgtgtctga cccacccaag
     2221 aatccatgct cgtatctaca aaaatgattt tttctcttgt aatggtgcct aggttctttt
     2281 attatcatgg catgtgtatg tttttcaact aggttacaat ctggccttat aaggttaacc
     2341 tcctggaggc caccagcctt cctgaaactt gtctgtgctg tccctgcaac tggagtgtgc
     2401 ctgatgtggc actccagcct ggacaagtgg gacacagact ccgctgttat caggcccaaa
     2461 gatgtcttcc ataagaccag aagagcaatg gtgtagaggt gtcatgggct acaataaaga
     2521 tgctgacctc ctgtctgagg gcaagcagcc tcttctggcc ctcagacaaa tgctgagtgt
     2581 tcccaagact accctcggcc tggtccaatc tcatcccact ggtgcgtaag ggttgctgaa
     2641 ctcatgactt cttggctagc ctgcaacctc cacggagtgg gaactacatc aggcattttg
     2701 ctaactgctg tatcctaggc caataaatgt tgatcacatt tatagctgcc atggtagggt
     2761 ggggacccct gctatctatc tgtggaggct ctgggagccc ctgacacaaa ctttctgaag
     2821 cagagcctcc ccaacccctt ttccattccc tatacctgac agatggccca ggaacccatt
     2881 agaaatggaa ggtcactgca gcagtatgtg aatgtgcgtg tgggagaagg gcaggatcag
     2941 agccctgggg gtgtggcagc ccccaagtga ttctaatcca gatcctaggg ttgtttccct
     3001 gtcccattga aatagctgct ttaaggggcc tgactcaggg aaatcagtct cttgaattaa
     3061 gtggtgattt tggagtcatt tagaccaggc cttcaattgg gatccggaga gctcgtcgac
     3121 ggcgcgtcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     3181 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     3241 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     3301 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     3361 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3421 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     3481 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     3541 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     3601 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     3661 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
     3721 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     3781 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     3841 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     3901 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     3961 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     4021 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4081 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4141 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4201 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4261 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4321 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4381 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4441 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4501 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4561 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4621 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4681 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4741 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4801 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4861 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4921 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4981 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5041 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5101 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5161 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5221 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5281 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5341 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5401 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5461 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5521 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5581 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5641 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5701 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5761 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5821 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gtcctgcagg
     5881 cagctgcgcg ctcgctcgct cact
//
