LOCUS       sequence-243891-       12608 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA in Aedes aegypti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12608)
  AUTHORS   Li M, Yang T, Kandul NP, Bui M, Gamez S, Raban R, Bennett J, Sanchez
            C HM, Lanzaro GC, Schmidt H, Lee Y, Marshall JM, Akbari OS
  TITLE     Development of a confinable gene drive system in the human disease 
            vector Aedes aegypti.
  JOURNAL   Elife. 2020 Jan 21;9. pii: 51701. doi: 10.7554/eLife.51701.
  PUBMED    31960794
REFERENCE   2  (bases 1 to 12608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-01-21"; volume: "9. pii: 51701. doi"; pages: " 
            10.7554/eLife.51701"
COMMENT     Sequence Label: U6b
FEATURES             Location/Qualifiers
     source          1..12608
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2998..3060
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      7081..7090
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8639..8760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             join(10502..10504,10505..10507,10508..11182)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     polyA_signal    11296..11417
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   12085..12119
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(12301..12317)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12301..12317)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12314..12336)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12325..12341
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12349..12355,12356..12373,12374..12379))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12394..12415
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12532..12549)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaatat aacaaaattg
     2341 tgatcccaca aaatgaagtg gggcaaaatc aaataattaa ctagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcg tgactataac acaagttgat
     2461 ttaataattt tagccaacac gtcgggctgc gtgttttttg cgctctgtgt acacgttgat
     2521 taactggtcg attaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     2581 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     2641 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     2701 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     2761 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     2821 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     2881 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     2941 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     3001 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     3061 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     3121 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     3181 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     3241 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     3301 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     3361 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
     3421 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
     3481 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
     3541 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
     3601 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
     3661 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
     3721 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
     3781 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
     3841 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
     3901 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
     3961 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
     4021 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccggggtcct aggcctcacg
     4081 ccactcgtag aatgaacccg attgaatcat ttttaaaccg aaaaaaaaat cctgccggtg
     4141 ttctctgagt tttctgagga ttctgatcgg gatcccagag gaacctggga attgctattg
     4201 gattcctaac agactcttga gaattaccat cagagtttcg tacattttta acaagatttt
     4261 ttgtggaatc atcaccaaac catgaaaatt tctagcaggc ttttggggtt cctcagtggg
     4321 atcatgaaac tccgttacat cgtttttgct atgagattac agaaaaaata acttaagtaa
     4381 acagtttttc tgtaaaagat atttcactca cttagctttt gaacgcagtg gaattttaga
     4441 ccattgtttg cttgactttt tttttaatca taatttaaat gttagagtgt tagaaaccgt
     4501 gttcttataa acaatattct ccttttcttc ttccctgtct tgcgaaacaa acgatagaga
     4561 gaaacttatc agtgtacctg gcgtctaagc tgcgaatcaa ggaactacga ttttccagcc
     4621 acttcctatg taaatcaatc gaagtatcgt ttagtacatt acatttaata catttcttct
     4681 tcatctatgc atctttgact tttccaaaat gaatatccta tttcattata cattttttct
     4741 tttacttcca gcgctacagc accagcagtt atcaggatgt agatgaaggt atcaacagtt
     4801 cgttcggcag caatgacaaa tccacgctga ttcaggtttg gaaacccaaa agttatggcg
     4861 ccgtcaaggg acaaattcca cagcacgaac gactcacata cacatggaag gagatcgatg
     4921 tgtttggaga gacgccaggt gataccaaaa aggaacccct gtgcagtaga ttgtgctgtt
     4981 gtttctcccg ccagaagaaa gacttcaatc cccgcaaaca cctactgaag aatgtgaccg
     5041 ggatggctaa gagtggagag ttactggctg tgatgggtag ttctggcgcc ggtaaaacaa
     5101 ccttgctgaa cgctttgcta gctcatcaca aacgaaacta ttataatcgt cttcagggga
     5161 acggtagcat catatctgtc accagtcagt cggattcctt tctttcggca taaattatgc
     5221 tcgggtgacg agacgagttg aaaatcccgt aaattaaaac atataccaca ctcgccgtcc
     5281 tagacgacga cgacgacgac gattcccaag gaattgaacc cttcatttgc atttcactac
     5341 cgctctgaga gatgcaaagc gcgccagaca gtcgatcgaa tctgcagacc agagagtcac
     5401 atcaagaatg ttgacattcc accagaagac tctaattatt cttcgctcga ctgggaaaac
     5461 tgttaacaac tgctcgagtg tggtcggagc ggctcacagt gcgttttccc gtagacaaaa
     5521 atcaaatttc atggtatttt tcggcgataa acagtaagtt aaagggaacc cgaatttagt
     5581 gctatataat ttaattccac tagagtttgt atcctttgat agatacgcgt atttcgacct
     5641 caactgcaag gccgtcgtgt actagacttg actaatccag actggtcttt tagttatgac
     5701 ttctgtccac atctccatac attcaacgca ctgtgcggct gtgctgtgcg actccgtcga
     5761 gtcgaccaac atagttgaaa caaattgaat atttaattga tcgttatagg aatggtgtta
     5821 gatgagtcat cctttacagt aagcacatac agtattataa ttgaagatcg tcggcagata
     5881 ggtgtgtagg gtagagtatc agcaataagt tgggacgttt gactttttgt aggtagacaa
     5941 aaactaaact ttttttcgct tctctatgtg tgcccctggg tagcgttccg ttccgattgg
     6001 ggtgcgaacg aatgaaatcg cccatcgagt tgatacgtcc atccatcgct agaaccgcgt
     6061 tcgctgtaga agactatata agagcagagg caagagtagt gaaatggtgc gatcttgacc
     6121 cccgggtctt agagctatgc tggaaacagc atagcaagtt aagataaggc tagtccgtta
     6181 tcaacttgaa aaagtggcac cgagtcggtg ctttttttgg tggttgttca tcgattccta
     6241 atattgcagt gaagcagttt gttgtgcatg gcgaagataa ggttagggcg aggaatgcct
     6301 tggaaataag cggcactggg ccgtatgaat aaaaagcgtt gaactttact acatgtagca
     6361 tctactcaaa aacaaaatcc acaaagttta ctacactttt ggtatgaata aaaacctttt
     6421 cgaacatgta gtacctacta ctttaagaaa attctattca aagtacagag taatgctgca
     6481 tgttaagaaa attccattca gagtgactct tgaaatcaat aaaatcatgc atatatgcca
     6541 aatttgtcac ttattcacgt gacaaattat gtgaaaatat gaatattact acattcccgc
     6601 tatgcatcgc gtaatcgatc atgaaattta tgttggttat gtaactttac agtattccct
     6661 ttatgtgcat gtgaatttta taatgatttg acaacaagga ttttccttag aattttcgaa
     6721 ttttagctag aaatctgtat cctatgaatg aatcttgaga taacagcaat tgtaacatta
     6781 taaagttcct aacattttca agattccagt attgagttaa ttaattcgag ctcgcccggg
     6841 gatctaattc aattagagac taattcaatt agagctaatt caattaggat ccaagcttat
     6901 cgatttcgaa ccctcgaccg ccggagtata aatagaggcg cttcgtctac ggagcgacaa
     6961 ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc
     7021 gcagctgaac aagctaaaca atcggggtac cgctagagtc gacggtacga tccaccggtc
     7081 gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc
     7141 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     7201 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     7261 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
     7321 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     7381 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
     7441 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
     7501 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
     7561 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
     7621 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
     7681 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
     7741 gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc
     7801 ggcaccgcct cctccgagga caacaacatg gccgttatca aggaatttat gcgcttcaaa
     7861 gttaggatgg agggatccat gaacggacat gagttcgaga tcgagggaga gggcgaggga
     7921 cgcccgtatg aaggcacaca aacagccaaa ctcaaggtca ccaagggcgg accactgccc
     7981 ttcgcctggg atatcctgag tccccagttt atgtacggca gcaaggccta cgttaagcac
     8041 cccgctgaca taccggacta caaaaagctg tcctttccgg aaggcttcaa gtgggagcgc
     8101 gtgatgaatt tcgaagacgg aggactggtc actgtgaccc aagatagcag tttgcaggac
     8161 ggtacactga tctataaggt taaaatgcgc ggcactaact ttccgccaga tggcccagtg
     8221 atgcagaaga agaccatggg ttgggaggca tccaccgaac gtctgtaccc tcgagacgga
     8281 gtgctcaagg gcgagatcca tcaggccctc aaactgaaag atggtggtca ctacctggtc
     8341 gaatttaaga ccatttacat ggccaagaag ccggttcagc tgcccggata ttattatgtg
     8401 gatacgaaac tggatataac ttcgcataac gaagactaca ccattgtcga gcagtatgag
     8461 cgcagcgaag gccgacatca cctgttcctc tacggcatgg acgagctgta caagtaggcg
     8521 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     8581 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     8641 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8701 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8761 aagcttgttt aaactcaaga tcgcaccgac ttcagtacgt gccctaaacg gaatcccagt
     8821 caacgcggaa cagttgcgag cacgttgcgc ctatgtgcaa caggacgatc ttttcattcc
     8881 cgcgcttaca actcgagaac acctcgtatt ccacgcaatg ctgcgtatgg gtaaagatgt
     8941 acccaagagt gtcaagatga atcgagtcaa cgaggtgctt caggagcttt ccttagcgaa
     9001 atgcgcagac acgatcattg gagcgccagg tcgaatgaaa ggcctgtccg gaggtgaacg
     9061 gaaacggttg gcctttgcat cggaaaccct gaccgatcct cacctgttgc tttgtgatga
     9121 acccacttcc gggttggatt cgttcatggc tcacagtgtt ctacaggtgc tgaagggaat
     9181 ggccctcaaa ggaaagacga ttatactgac catacatcaa ccttcgtcag aactgtattg
     9241 cttgttcgac aaaattctcc tagtggcaga aggacgcgtg gctttccttg gatctcccta
     9301 tcaggcttcg gaattctttt ctcagtgagt agacttgtga aatcctgacg attttgcttt
     9361 actagcaggg cttgtggtgt cttgcctctt aaaacaccga acatgaaacc caaaagccaa
     9421 acgcaatagg aatcagacga tgagagttct tcatagatct gaccagacat gtctctaaga
     9481 atatttctaa gacttttttt ttggaaattc atcgcgatct tgacattacg cgtaatccgc
     9541 gtaatcatcg tatttttcca aagttacatt cagatattac ggcagaaatt ctttaggaga
     9601 tttcatcagg agataacata ttggtgaatt acggattcgt tgtatttatc ttcaagaata
     9661 ctacctgatt ttagtttagc aattacttca gagatttcat ttagaattca agattcatgc
     9721 agaaatattc tttcattgat agaacttcct tctatgcaga acatctacca gaattcaacc
     9781 agaaatttct acttatatac ctctaacacg gccaattgcg gccagacagt aaaataatag
     9841 tggccttagt aactaaaatg atcaaaattg tgacccggta aagtgattta ataaaacagt
     9901 ttttagtcca aacaatatta ggggtcatcc aaaaacgact tcaatcatta ggggcgcgcc
     9961 catactcggt ggcctcccca ccaccaactt ttttgcactg caaaaaaaca cgcttttgca
    10021 cgcgggccca tacatagtac aaactctacg tttcgtagac tattttacat aaatagtcta
    10081 caccgttgta tacgctccaa atacactacc acacattgaa cctttttgca gtgcaaaaaa
    10141 gtacgtgtcg gcagtcacgt aggccggcct tatcgggtcg cgtcctgtca cgtacgaatc
    10201 acattatcgg accggacgag tgttgtctta tcgtgacagg acgccagctt cctgtgttgc
    10261 taaccgcagc cggacgcaac tccttatcgg aacaggacgc gcctccatat cagccgcgcg
    10321 ttatctcatg cgcgtgaccg gacacgaggc gcccgtcccg cttatcgcgc ctataaatac
    10381 agcccgcaac gatctggtaa acacagttga acagcatctg ttacagcgac acaacatgag
    10441 ccggtccaac aacgccaacg cgcccacgcc atccaaccgc cgccgcaacc tgtctctggt
    10501 gatggtgcgc tcctccaaga acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga
    10561 gggcaccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
    10621 gggccacaac accgtgaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
    10681 catcctgtcc ccccagttcc agtacggctc caaggtgtac gtgaagcacc ccgccgacat
    10741 ccccgactac aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
    10801 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gctgcttcat
    10861 ctacaaggtg aagttcatcg gcgtgaactt cccctccgac ggccccgtaa tgcagaagaa
    10921 gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg
    10981 cgagatccac aaggccctga agctgaagga cggcggccac tacctggtgg agttcaagtc
    11041 catctacatg gccaagaagc ccgtgcagct gcccggctac tactacgtgg actccaagct
    11101 ggacatcacc tcccacaacg aggactacac catcgtggag cagtacgagc gcaccgaggg
    11161 ccgccaccac ctgttcctgt aggactctag atcataatca gccataccac atttgtagag
    11221 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    11281 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    11341 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    11401 ctcatcaatg tatcttaaag cttctaggcc ggccgatctc ggatctgaca atgttcagtg
    11461 cagagactcg gctacgcctc gtggactttg aagttgacca acaatgttta ttcttacctc
    11521 taatagtcct ctgtggcaag gtcaagattc tgttagaagc caatgaagaa cctggttgtt
    11581 caataacatt ttgttcgtct aatatttcac taccgcttga cgttggctgc acttcatgta
    11641 cctcatctat aaacgcttct tctgtatcgc tctggacgtc atcttcactt acgtgatctg
    11701 atatttcact gtcagaatcc tcaccaacaa gctcgtcatc gctttgcaga agagcagaga
    11761 ggatatgctc atcgtctaaa gaactaccca ttttattata tattagtcac gatatctata
    11821 acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata acaatataat
    11881 tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca
    11941 cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg
    12001 agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa
    12061 agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt
    12121 taaaaaagat ttgcgcttta ctcgacctaa actttaaaca cgtcatagaa tcttcgtttg
    12181 acaaaaacca cattgtggcc aagctgtgtg acgcgacgcg cgctaaagaa tggcaaacca
    12241 agtcgcgcga gcgtcgactc tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg
    12301 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    12361 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    12421 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    12481 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    12541 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    12601 aatacggt
//
