LOCUS       sequence-243895-       10386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tuning expression of cytosolic Ovalbumin coupled to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10386)
  AUTHORS   Michaels YS, Barnkob MB, Barbosa H, Baeumler TA, Thompson MK, Andre 
            V, Colin-York H, Fritzsche M, Gileadi U, Sheppard HM, Knapp DJHF, 
            Milne TA, Cerundolo V, Fulga TA
  TITLE     Precise tuning of gene expression levels in mammalian cells.
  JOURNAL   Nat Commun. 2019 Feb 18;10(1):818. doi: 10.1038/s41467-019-08777-y.
  PUBMED    30778069
REFERENCE   2  (bases 1 to 10386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-02-18"; volume: "10(1):818. doi"; pages: " 
            10.1038/s41467-019-08777-y"
COMMENT     Sequence Label: OVA-miSFIT-8-G
FEATURES             Location/Qualifiers
     source          1..10386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1751..2206)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1888..1909)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2100..2119
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2333..2355
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2347..2364
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2348..2364
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2374..2393
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2374..2392
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2413..2548
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2588..2709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             2781..3014
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3101..3689
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3207..3218
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3616..3636
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     promoter        complement(4693..5203)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4756..4774)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        5231..5269
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             5988..6011
                     /codon_start=1
                     /product="antigenic CD8+ T cell epitope from chicken 
                     ovalbumin"
                     /label=OVA peptide
                     /translation="SIINFEKL"
     CDS             6390..6443
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6444..6446,6447..7157)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6444..6446,6447..7157)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6486..6507)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6747..6766)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7094..7115
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7285..7419
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    7556..7673
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7940..7984)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8169..8402
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8895..9020
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9067..9247
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(9262..9465)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        9466..9845
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(9946..9966)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9946..9964)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9985..10001)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9985..10001)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9998..10020)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10009..10025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10033..10039,10040..10057,10058..10063))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10078..10099
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10216..10233)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     1801 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     1861 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     1921 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     1981 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     2041 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     2101 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     2161 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     2221 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     2281 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     2341 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     2401 ggtacgtccc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     2461 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     2521 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggata gctagagcca
     2581 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     2641 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     2701 aaacaagttc ctctcactct ctgatattca tttctttgca agttataaat actgaataat
     2761 aagatgacat gaactactac tgctagagat tttccacact gactaaaagg gtctgaggga
     2821 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     2881 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     2941 ctggtctaac cagagagacc cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg
     3001 aattagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt
     3061 gtaagtcatt ggtcttaaag gtaccgagct cgaattccag gcggggaggc ggcccaaagg
     3121 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
     3181 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
     3241 ccgcgcagaa tccaggtggc aacacaggcg agcagccatg gaaaggacgt cagcttcccc
     3301 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
     3361 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
     3421 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
     3481 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     3541 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     3601 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     3661 tcacaaattt tgtaatccag aggttgattg tcgactctag actcgactct agaggatccc
     3721 cctgttccac ctcttgaagg ctatgtaggc cacaaggccc acaaccacag cagccaggat
     3781 ggagcaatag acagggatga ggttgtcggt ggtgcctcgg gtcaccacgg gctgggagct
     3841 gcccatcact gtggtcacca cacctgccac cgtgctggct atgaggtctt gttctggagg
     3901 tgcctcaggc tcctgggtgc tgggggctgt gctgtccgag ccctctgggg gtgtggaccg
     3961 tgtaatccaa cggccaggga tctcctcgca ctcggcgtcg gcccagcgtg tgcactcgcg
     4021 gagctggcgc tcggtgtcct cgcacacggt gcagggcagg cacgggtcca cgtggttggc
     4081 ctcgtcggaa tacgtgccgt cggggcactc ctcgcacacg gtgttctgct tgtcctggca
     4141 ggagaacacg aggcccgagc ccgcctcgca cacgcggcac gcctcgcagc gcccagtcgt
     4201 ctcatcctgg tagtagccgt aggcgcagcg gcacacggcg tcgtcggcct ccacgcacgg
     4261 cgccgacatg ctctggagcc ccacgcactc ggtgcacggc ttgcacggct cggtcgcgct
     4321 caccacgtcg gagaacgtca cgctgtccag gcagggctca cacacggtct ggttggctcc
     4381 acaaggctgg gccacaccct cgcccaggtt gcaggctttg cagcactcac cgctgtgtgt
     4441 gtacaggcct gtggggcatg cctccttggc acctccaagg gacaccccca gaagcagcaa
     4501 cagcagcagg cgcggcccgt ccatggcgcg gccggtggca cctgccccca tcgcccgcct
     4561 cccgcggcag cgctcgactt ccagctcggt ccgctttgcg gactgatggg gctgcgctgc
     4621 gctgcgctcc agcgcccccc ctgcccgccg gagctggccg cggcccgaat tcctgcagcc
     4681 cgggggatcc ccctggggag agaggtcggt gattcggtca acgagggagc cgactgccga
     4741 cgtgcgctcc ggaggcttgc agaatgcgga acaccgcgcg ggcaggaaca gggcccacac
     4801 taccgcccca caccccgcct cccgcaccgc cccttcccgg ccgctgctct cggcgcgccc
     4861 tgctgagcag ccgctattgg ccacagccca tcgcggtcgg cgcgctgcca ttgctccctg
     4921 gcgctgtccg tctgcgaggg tactagtgag acgtgcggct tccgtttgtc acgtccggca
     4981 cgccgcgaac cgcaaggaac cttcccgact taggggcgga gcaggaagcg tcgccggggg
     5041 gcccacaagg gtagcggcga agatccgggt gacgctgcga acggacgtga agaatgtgcg
     5101 agacccaggg tcggcgccgc tgcgtttccc ggaaccacgc ccagagcagc cgcgtccctg
     5161 cgcaaaccca gggctgcctt ggaaaaggcg caaccccaac cccgtggaat tcgatatcaa
     5221 gcttctcgag ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
     5281 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggag
     5341 ctcctgcagg tgccgccatg agcaccagga cacaaataaa taaggttgtt cgctttgata
     5401 aacttccagg attcggagac agtattgaag ctcagtgtgg cacatctgta aacgttcact
     5461 cttcacttag agacatcctc aaccaaatca ccaaaccaaa tgatgtttat tcgttcagcc
     5521 ttgccagtag actttatgct gaagagagat acccaatcct gccagaatac ttgcagtgtg
     5581 tgaaggaact gtatagagga ggcttggaac ctatcaactt tcaaacagct gcagatcaag
     5641 ccagagagct catcaattcc tgggtagaaa gtcagacaaa tggaattatc agaaatgtcc
     5701 ttcagccaag ctccgtggat tctcaaactg caatggttct ggttaatgcc attgtcttca
     5761 aaggactgtg ggagaaagca tttaaggatg aagacacaca agcaatgcct ttcagagtga
     5821 ctgagcaaga aagcaaacct gtgcagatga tgtaccagat tggtttattt agagtggcat
     5881 caatggcttc tgagaaaatg aagatcctgg agcttccatt tgccagtggg acaatgagca
     5941 tgttggtgct gttgcctgat gaagtctcag gccttgagca gcttgagagt ataatcaact
     6001 ttgaaaaact gactgaatgg accagttcta atgttatgga agagaggaag atcaaagtgt
     6061 acttacctcg catgaagatg gaggaaaaat acaacctcac atctgtctta atggctatgg
     6121 gcattactga cgtgtttagc tcttcagcca atctgtctgg catctcctca gcagagagcc
     6181 tgaagatatc tcaagctgtc catgcagcac atgcagaaat caatgaagca ggcagagagg
     6241 tggtagggtc agcagaggct ggagtggatg ctgcaagcgt ctctgaagaa tttagggctg
     6301 accatccatt cctcttctgt atcaagcaca tcgcaaccaa cgccgttctc ttctttggca
     6361 gatgtgtttc ccctgaattc ggcagtggag agggcagagg aagtctgcta acatgcggtg
     6421 acgtcgagga gaatcctggc ccagtgagca agggcgagga gctgttcacc ggggtggtgc
     6481 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     6541 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     6601 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     6661 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     6721 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     6781 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     6841 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     6901 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     6961 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     7021 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     7081 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     7141 tggacgagct gtacaaggaa ttctaactag agctcgcacg tgagtgctag cctacctgga
     7201 ctgtaagcac tttgaccggt actagcttag tcatgcaccg ggctgcagga attcgatgac
     7261 aggcgcagct tccgagggat ttgagatcca gacatgataa gatacattga tgagtttgga
     7321 caaaccaaaa ctagaatgca gtgaaaaaaa tgccttattt gtgaaatttg tgatgctatt
     7381 gccttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     7441 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     7501 aaatgtggta tggctgatta tgatcagatc tctcgaggct agtctcgtga tcgataaaat
     7561 tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt
     7621 ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta aaagttaacc
     7681 gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
     7741 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
     7801 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
     7861 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
     7921 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
     7981 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
     8041 taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat
     8101 tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc
     8161 aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga
     8221 gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca
     8281 gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg
     8341 tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc
     8401 ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta
     8461 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
     8521 tcatatctcc tcctccaggt ctgaagatca gcggccgctt gctgtgcggt ggtcttactt
     8581 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
     8641 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
     8701 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
     8761 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
     8821 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
     8881 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
     8941 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
     9001 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
     9061 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
     9121 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
     9181 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
     9241 gagaccccgg ttcactaaac gagctctgct tatatagacc tcccaccgta cacgcctacc
     9301 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
     9361 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
     9421 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
     9481 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
     9541 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
     9601 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
     9661 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
     9721 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
     9781 atatgggcta tgaactaatg accccgtaat tgattactat taataactag tcaataatca
     9841 atgtcaacat ggcggtaatg ttggacatga gccaatataa atgtacatat tatgatatgg
     9901 atacaacgta tgcaatggcc aagcttgcag ctccagcttt tgttcccttt agtgagggtt
     9961 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    10021 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    10081 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    10141 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    10201 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    10261 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    10321 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    10381 ggcgtt
//
