LOCUS       sequence-243905-        5855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vimentin labeling with near-infrared fluorescent protein 
            miRFP670nano.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Oliinyk OS, Shemetov AA, Pletnev S, Shcherbakova DM, Verkhusha VV
  TITLE     Smallest near-infrared fluorescent protein evolved from 
            cyanobacteriochrome as versatile tag for spectral multiplexing.
  JOURNAL   Nat Commun. 2019 Jan 17;10(1):279. doi: 10.1038/s41467-018-08050-8.
  PUBMED    30655515
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-17"; volume: "10(1):279. doi"; pages: " 
            10.1038/s41467-018-08050-8"
COMMENT     Sequence Label: pVimentin-miRFP670nano
FEATURES             Location/Qualifiers
     source          1..5855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        221..424
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     375..395
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2499..2620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2536..2555)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2590..2609
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2627..3082)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2764..2785)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2976..2995
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3109..3213
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3215..3572
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3215..3406
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3238..3258)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3423..3558
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3607..4401
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3607..4401
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3661..3680)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4271..4290
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4589..4608)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4633..4680
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5009..5597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5498..5517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        join(5772..5855,1..220)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
       61 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      121 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      181 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      241 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      301 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      361 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      421 agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag
      481 cttcgaattc ccatgtccac caggtccgtg tcctcgtcct cctaccgcag gatgttcggc
      541 ggcccgggca ccgcgagccg gccgagctcc agccggagct acgtgactac gtccacccgc
      601 acctacagcc tgggcagcgc gctgcgcccc agcaccagcc gcagcctcta cgcctcgtcc
      661 ccgggcggcg tgtatgccac gcgctcctct gccgtgcgcc tgcggagcag cgtgcccggg
      721 gtgcggctcc tgcaggactc ggtggacttc tcgctggccg acgccatcaa caccgagttc
      781 aagaacaccc gcaccaacga gaaggtggag ctgcaggagc tgaatgaccg cttcgccaac
      841 tacatcgaca aggtgcgctt cctggagcag cagaataaga tcctgctggc cgagctcgag
      901 cagctcaagg gccaaggcaa gtcgcgcctg ggggacctct acgaggagga gatgcgggag
      961 ctgcgccggc aggtggacca gctaaccaac gacaaagccc gcgtcgaggt ggagcgcgac
     1021 aacctggccg aggacatcat gcgcctccgg gagaaattgc aggaggagat gcttcagaga
     1081 gaggaagccg aaaacaccct gcaatctttc agacaggatg ttgacaatgc gtctctggca
     1141 cgtcttgacc ttgaacgcaa agtggaatct ttgcaagaag agattgcctt tttgaagaaa
     1201 ctccacgaag aggaaatcca ggagctgcag gctcagattc aggaacagca tgtccaaatc
     1261 gatgtggatg tttccaagcc tgacctcacg gctgccctgc gtgacgtacg tcagcaatat
     1321 gaaagtgtgg ctgccaagaa cctgcaggag gcagaagaat ggtacaaatc caagtttgct
     1381 gacctctctg aggctgccaa ccggaacaat gacgccctgc gccaggcaaa gcaggagtcc
     1441 actgagtacc ggagacaggt gcagtccctc acctgtgaag tggatgccct taaaggaacc
     1501 aatgagtccc tggaacgcca gatgcgtgaa atggaagaga actttgccgt tgaagctgct
     1561 aactaccaag acactattgg ccgcctgcag gatgagattc agaatatgaa ggaggaaatg
     1621 gctcgtcacc ttcgtgaata ccaagacctg ctcaatgtta agatggccct tgacattgag
     1681 attgccacct acaggaagct gctggaaggc gaggagagca ggatttctct gcctcttcca
     1741 aacttttcct ccctgaacct gagggaaact aatctggatt cactccctct ggttgatacc
     1801 cactcaaaaa ggacacttct gattaagacg gttgaaacta gagatggaca ggttatcaac
     1861 gaaacttctc agcatcacga tgaccttgaa ggggatccac cggtaatggc aaacctggac
     1921 aagatgctga ataccacagt aacagaggtg cggcagttcc tgcaggtgga cagagtgtgc
     1981 gtgttccagt ttgaggagga ttatagcgga gtggtggtgg tggaggccgt ggacgatagg
     2041 tggatctcca tcctgaagac ccaggtgcgg gatagatact tcatggagac aaggggcgag
     2101 gagtattctc acggccgcta ccaggccatc gccgacatct acaccgcaaa cctgacagag
     2161 tgctacaggg atctgctgac acagtttcag gtgagagcaa tcctggccgt gcccatcctg
     2221 cagggcaaga agctgtgggg cctgttggtg gcacaccagc tggcggcccc tagacagtgg
     2281 cagacctggg agatcgactt tctgaagcag caggccgtgg tggtgggcat cgccatccag
     2341 cagagctagc tgaattctgc agatatccag cacagtggcg gccgcgactc tagatcataa
     2401 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     2461 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     2521 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     2581 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt
     2641 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     2701 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     2761 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     2821 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     2881 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     2941 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     3001 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     3061 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3121 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3181 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
     3241 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3301 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     3361 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     3421 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     3481 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     3541 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
     3601 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     3661 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     3721 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     3781 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     3841 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     3901 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     3961 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     4021 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     4081 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     4141 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     4201 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     4261 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     4321 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     4381 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     4441 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     4501 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     4561 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     4621 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
     4681 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     4741 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     4801 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
     4861 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4921 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4981 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5041 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5101 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5161 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5221 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5281 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5341 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5401 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5461 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5521 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5581 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5641 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5701 ccgccatgca ttagttatta atagtaatca attacggggt cattagttca tagcccatat
     5761 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     5821 ccccgcccat tgacgtcaat aatgacgtat gttcc
//
