LOCUS       sequence-243916-        9445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tuning expression of PD-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Michaels YS, Barnkob MB, Barbosa H, Baeumler TA, Thompson MK, Andre 
            V, Colin-York H, Fritzsche M, Gileadi U, Sheppard HM, Knapp DJHF, 
            Milne TA, Cerundolo V, Fulga TA
  TITLE     Precise tuning of gene expression levels in mammalian cells.
  JOURNAL   Nat Commun. 2019 Feb 18;10(1):818. doi: 10.1038/s41467-019-08777-y.
  PUBMED    30778069
REFERENCE   2  (bases 1 to 9445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-02-18"; volume: "10(1):818. doi"; pages: " 
            10.1038/s41467-019-08777-y"
COMMENT     Sequence Label: PD-L1-miSFIT-1x
FEATURES             Location/Qualifiers
     source          1..9445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1751..2206)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1888..1909)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2100..2119
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2333..2355
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2347..2364
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2348..2364
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2374..2393
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2374..2392
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2413..2548
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2588..2709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             2781..3014
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3101..3689
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3207..3218
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3616..3636
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     promoter        complement(4693..5203)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4756..4774)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        5231..5269
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    6344..6478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6615..6732
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6999..7043)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7228..7461
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7954..8079
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8126..8306
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8321..8524)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        8525..8904
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(9005..9025)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9005..9023)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9044..9060)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9044..9060)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9057..9079)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9068..9084
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9092..9098,9099..9116,9117..9122))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9137..9158
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9275..9292)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     1801 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     1861 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     1921 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     1981 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     2041 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     2101 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     2161 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     2221 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     2281 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     2341 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     2401 ggtacgtccc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     2461 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     2521 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggata gctagagcca
     2581 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     2641 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     2701 aaacaagttc ctctcactct ctgatattca tttctttgca agttataaat actgaataat
     2761 aagatgacat gaactactac tgctagagat tttccacact gactaaaagg gtctgaggga
     2821 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     2881 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     2941 ctggtctaac cagagagacc cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg
     3001 aattagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt
     3061 gtaagtcatt ggtcttaaag gtaccgagct cgaattccag gcggggaggc ggcccaaagg
     3121 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
     3181 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
     3241 ccgcgcagaa tccaggtggc aacacaggcg agcagccatg gaaaggacgt cagcttcccc
     3301 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
     3361 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
     3421 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
     3481 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     3541 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     3601 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     3661 tcacaaattt tgtaatccag aggttgattg tcgactctag actcgactct agaggatccc
     3721 cctgttccac ctcttgaagg ctatgtaggc cacaaggccc acaaccacag cagccaggat
     3781 ggagcaatag acagggatga ggttgtcggt ggtgcctcgg gtcaccacgg gctgggagct
     3841 gcccatcact gtggtcacca cacctgccac cgtgctggct atgaggtctt gttctggagg
     3901 tgcctcaggc tcctgggtgc tgggggctgt gctgtccgag ccctctgggg gtgtggaccg
     3961 tgtaatccaa cggccaggga tctcctcgca ctcggcgtcg gcccagcgtg tgcactcgcg
     4021 gagctggcgc tcggtgtcct cgcacacggt gcagggcagg cacgggtcca cgtggttggc
     4081 ctcgtcggaa tacgtgccgt cggggcactc ctcgcacacg gtgttctgct tgtcctggca
     4141 ggagaacacg aggcccgagc ccgcctcgca cacgcggcac gcctcgcagc gcccagtcgt
     4201 ctcatcctgg tagtagccgt aggcgcagcg gcacacggcg tcgtcggcct ccacgcacgg
     4261 cgccgacatg ctctggagcc ccacgcactc ggtgcacggc ttgcacggct cggtcgcgct
     4321 caccacgtcg gagaacgtca cgctgtccag gcagggctca cacacggtct ggttggctcc
     4381 acaaggctgg gccacaccct cgcccaggtt gcaggctttg cagcactcac cgctgtgtgt
     4441 gtacaggcct gtggggcatg cctccttggc acctccaagg gacaccccca gaagcagcaa
     4501 cagcagcagg cgcggcccgt ccatggcgcg gccggtggca cctgccccca tcgcccgcct
     4561 cccgcggcag cgctcgactt ccagctcggt ccgctttgcg gactgatggg gctgcgctgc
     4621 gctgcgctcc agcgcccccc ctgcccgccg gagctggccg cggcccgaat tcctgcagcc
     4681 cgggggatcc ccctggggag agaggtcggt gattcggtca acgagggagc cgactgccga
     4741 cgtgcgctcc ggaggcttgc agaatgcgga acaccgcgcg ggcaggaaca gggcccacac
     4801 taccgcccca caccccgcct cccgcaccgc cccttcccgg ccgctgctct cggcgcgccc
     4861 tgctgagcag ccgctattgg ccacagccca tcgcggtcgg cgcgctgcca ttgctccctg
     4921 gcgctgtccg tctgcgaggg tactagtgag acgtgcggct tccgtttgtc acgtccggca
     4981 cgccgcgaac cgcaaggaac cttcccgact taggggcgga gcaggaagcg tcgccggggg
     5041 gcccacaagg gtagcggcga agatccgggt gacgctgcga acggacgtga agaatgtgcg
     5101 agacccaggg tcggcgccgc tgcgtttccc ggaaccacgc ccagagcagc cgcgtccctg
     5161 cgcaaaccca gggctgcctt ggaaaaggcg caaccccaac cccgtggaat tcgatatcaa
     5221 gcttctcgag ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
     5281 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggag
     5341 ctcctgcagg ccgccatgag gatatttgct gtctttatat tcatgaccta ctggcatttg
     5401 ctgaacgcat ttactgtcac ggttcccaag gacctatatg tggtagagta tggtagcaat
     5461 atgacaattg aatgcaaatt cccagtagaa aaacaattag acctggctgc actaattgtc
     5521 tattgggaaa tggaggataa gaacattatt caatttgtgc atggagagga agacctgaag
     5581 gttcagcata gtagctacag acagagggcc cggctgttga aggaccagct ctccctggga
     5641 aatgctgcac ttcagatcac agatgtgaaa ttgcaggatg caggggtgta ccgctgcatg
     5701 atcagctatg gtggtgccga ctacaagcga attactgtga aagtcaatgc cccatacaac
     5761 aaaatcaacc aaagaatttt ggttgtggat ccagtcacct ctgaacatga actgacatgt
     5821 caggctgagg gctaccccaa ggccgaagtc atctggacaa gcagtgacca tcaagtcctg
     5881 agtggtaaga ccaccaccac caattccaag agagaggaga agcttttcaa tgtgaccagc
     5941 acactgagaa tcaacacaac aactaatgag attttctact gcacttttag gagattagat
     6001 cctgaggaaa accatacagc tgaattggtc atcccagaac tacctctggc acatcctcca
     6061 aatgaaagga ctcacttggt aattctggga gccatcttat tatgccttgg tgtagcactg
     6121 acattcatct tccgtttaag aaaagggaga atgatggatg tgaaaaaatg tggcatccaa
     6181 gatacaaact caaagaagca aagtgataca catttggagg agacctaagg ggtagcacgt
     6241 gagtgctagc ctacctgcac tgtaagcact ttgaccggta ctagcttagt catgcaccgg
     6301 gctgcaggaa ttcgatgaca ggcgcagctt ccgagggatt tgagatccag acatgataag
     6361 atacattgat gagtttggac aaaccaaaac tagaatgcag tgaaaaaaat gccttatttg
     6421 tgaaatttgt gatgctattg ccttatttgt aaccattata agctgcaata aacaagttaa
     6481 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     6541 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagatct ctcgaggcta
     6601 gtctcgtgat cgataaaatt ttgaattttt gtaatttgtt tttgtaattc tttagtttgt
     6661 atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc aatcccccct
     6721 tttcttttaa aagttaaccg ataccgtcga gatccgttca ctaatcgaat ggatctgtct
     6781 ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt
     6841 gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa
     6901 agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat gaataatttt
     6961 atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat
     7021 tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt
     7081 attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa
     7141 atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg
     7201 agttttccag agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca
     7261 gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt
     7321 tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt
     7381 ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag
     7441 aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc
     7501 tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat
     7561 tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag cggccgcttg
     7621 ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt
     7681 gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga
     7741 tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca
     7801 gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc
     7861 ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc
     7921 ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt
     7981 ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc
     8041 cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc
     8101 tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg
     8161 agggatctct agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa
     8221 ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct
     8281 cagatctggt ctaaccagag agaccccggt tcactaaacg agctctgctt atatagacct
     8341 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
     8401 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
     8461 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
     8521 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
     8581 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     8641 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     8701 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
     8761 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     8821 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
     8881 aataactagt caataatcaa tgtcaacatg gcggtaatgt tggacatgag ccaatataaa
     8941 tgtacatatt atgatatgga tacaacgtat gcaatggcca agcttgcagc tccagctttt
     9001 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     9061 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9121 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9181 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9241 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9301 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9421 gtaaaaaggc cgcgttgctg gcgtt
//
