LOCUS       sequence-243917-        4457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Monomeric near-infrared fluorescent protein miRFP670nano in 
            bacterial plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Oliinyk OS, Shemetov AA, Pletnev S, Shcherbakova DM, Verkhusha VV
  TITLE     Smallest near-infrared fluorescent protein evolved from 
            cyanobacteriochrome as versatile tag for spectral multiplexing.
  JOURNAL   Nat Commun. 2019 Jan 17;10(1):279. doi: 10.1038/s41467-018-08050-8.
  PUBMED    30655515
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-17"; volume: "10(1):279. doi"; pages: " 
            10.1038/s41467-018-08050-8"
COMMENT     Sequence Label: pBAD/His-miRFP670nano
FEATURES             Location/Qualifiers
     source          1..4457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        853..1137
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1060..1079
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1180..1197
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1710..1727)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1880..1966
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2058..2085
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2104..2195
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2196..2264,2265..3056)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2414..2433)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3227..3815
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3716..3735
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3969..3986
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4001..4141
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4067..4086)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4227..4249
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(4405..4457,1..826))
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt
       61 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct
      121 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc
      181 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat
      241 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat
      301 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa
      361 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt
      421 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat
      481 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa
      541 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg
      601 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca
      661 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga
      721 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat
      781 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc
      841 cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct
      901 tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac
      961 aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa
     1021 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca
     1081 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc
     1141 cgttttttgg gctaacagga ggaattaacc atggggagcc accatcacca tcaccatggc
     1201 agatctgcag ctggtaccat ggcaaacctg gacaagatgc tgaataccac agtaacagag
     1261 gtgcggcagt tcctgcaggt ggacagagtg tgcgtgttcc agtttgagga ggattatagc
     1321 ggagtggtgg tggtggaggc cgtggacgat aggtggatct ccatcctgaa gacccaggtg
     1381 cgggatagat acttcatgga gacaaggggc gaggagtatt ctcacggccg ctaccaggcc
     1441 atcgccgaca tctacaccgc aaacctgaca gagtgctaca gggatctgct gacacagttt
     1501 caggtgagag caatcctggc cgtgcccatc ctgcagggca agaagctgtg gggcctgttg
     1561 gtggcacacc agctggcggc ccctagacag tggcagacct gggagatcga ctttctgaag
     1621 cagcaggccg tggtggtggg catcgccatc cagcagagct agctgaattc gaagcttggc
     1681 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
     1741 ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1801 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
     1861 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1921 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
     1981 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
     2041 caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac
     2101 tcttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2161 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2221 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2281 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2341 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2401 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     2461 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2521 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2581 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2641 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2701 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2761 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2821 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2881 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     2941 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3001 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3061 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3121 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3181 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3241 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3301 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3361 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3421 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3481 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3541 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3601 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3661 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3721 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3781 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3841 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3901 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3961 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     4021 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     4081 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     4141 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     4201 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     4261 atcaccgaaa cgcgcgaggc agcagatcaa ttcgcgcgcg aaggcgaagc ggcatgcata
     4321 atgtgcctgt caaatggacg aagcagggat tctgcaaacc ctatgctact ccgtcaagcc
     4381 gtcaattgtc tgattcgtta ccaattatga caacttgacg gctacatcat tcactttttc
     4441 ttcacaaccg gcacgga
//
