LOCUS       sequence-243923-       13440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses multimeric human VWF protein without A1 domain and a 
            D1498A mutation in A2 domain. This protein has C-terminal c-Myc 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13440)
  AUTHORS   Zhang C, Kelkar A, Neelamegham S
  TITLE     von Willebrand factor self-association is regulated by the 
            shear-dependent unfolding of the A2 domain.
  JOURNAL   Blood Adv. 2019 Apr 9;3(7):957-968. doi: 
            10.1182/bloodadvances.2018030122.
  PUBMED    30936056
REFERENCE   2  (bases 1 to 13440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/bloodadvances"; journalName: "Blood Adv"; date: "2019-04-9-
            9"; volume: "3"; issue: "7"; pages: "957-968"
COMMENT     Sequence Label: pcDNA3.1-deltaA1-D1498A-VWF
FEATURES             Location/Qualifiers
     source          1..13440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        56..435
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        436..639
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     590..610
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     684..703
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        684..702
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2361..2372
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             8626..8655
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(8683..8700)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8689..8913
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8959..9387
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9046..9065)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9256..9277
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9396..9416)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9401..9730
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9581..9716
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9643..9662
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9779..10804
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    10934..11055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10971..10990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11025..11044
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11104..11120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11104..11120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11117..11139)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11128..11144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11152..11158,11159..11176,11177..11182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11197..11218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11335..11352)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11506..12094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11586..11605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12265..13056,13057..13125))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12888..12907
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13126..13230)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13305..13324)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
       61 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      121 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      181 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      241 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      301 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      361 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      421 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      481 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      541 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      601 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
      661 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
      721 cgtttaaact taagctgatc cactagtcca gtgtggtgga attcccgcag ccctcatttg
      781 caggggaaga tgattcctgc cagatttgcc ggggtgctgc ttgctctggc cctcattttg
      841 ccagggaccc tttgtgcaga aggaactcgc ggcaggtcat ccacggcccg atgcagcctt
      901 ttcggaagtg acttcgtcaa cacctttgat gggagcatgt acagctttgc gggatactgc
      961 agttacctcc tggcaggggg ctgccagaaa cgctccttct cgattattgg ggacttccag
     1021 aatggcaaga gagtgagcct ctccgtgtat cttggggaat tttttgacat ccatttgttt
     1081 gtcaatggta ccgtgacaca gggggaccaa agagtctcca tgccctatgc ctccaaaggg
     1141 ctgtatctag aaactgaggc tgggtactac aagctgtccg gtgaggccta tggctttgtg
     1201 gccaggatcg atggcagcgg caactttcaa gtcctgctgt cagacagata cttcaacaag
     1261 acctgcgggc tgtgtggcaa ctttaacatc tttgctgaag atgactttat gacccaagaa
     1321 gggaccttga cctcggaccc ttatgacttt gccaactcat gggctctgag cagtggagaa
     1381 cagtggtgtg aacgggcatc tcctcccagc agctcatgca acatctcctc tggggaaatg
     1441 cagaagggcc tgtgggagca gtgccagctt ctgaagagca cctcggtgtt tgcccgctgc
     1501 caccctctgg tggaccccga gccttttgtg gccctgtgtg agaagacttt gtgtgagtgt
     1561 gctggggggc tggagtgcgc ctgccctgcc ctcctggagt acgcccggac ctgtgcccag
     1621 gagggaatgg tgctgtacgg ctggaccgac cacagcgcgt gcagcccagt gtgccctgct
     1681 ggtatggagt ataggcagtg tgtgtcccct tgcgccagga cctgccagag cctgcacatc
     1741 aatgaaatgt gtcaggagcg atgcgtggat ggctgcagct gccctgaggg acagctcctg
     1801 gatgaaggcc tctgcgtgga gagcaccgag tgtccctgcg tgcattccgg aaagcgctac
     1861 cctcccggca cctccctctc tcgagactgc aacacctgca tttgccgaaa cagccagtgg
     1921 atctgcagca atgaagaatg tccaggggag tgccttgtca caggtcaatc acacttcaag
     1981 agctttgaca acagatactt caccttcagt gggatctgcc agtacctgct ggcccgggat
     2041 tgccaggacc actccttctc cattgtcatt gagactgtcc agtgtgctga tgaccgcgac
     2101 gctgtgtgca cccgctccgt caccgtccgg ctgcctggcc tgcacaacag ccttgtgaaa
     2161 ctgaagcatg gggcaggagt tgccatggat ggccaggacg tccagctccc cctcctgaaa
     2221 ggtgacctcc gcatccagca tacagtgacg gcctccgtgc gcctcagcta cggggaggac
     2281 ctgcagatgg actgggatgg ccgcgggagg ctgctggtga agctgtcccc cgtctatgcc
     2341 gggaagacct gcggcctgtg tgggaattac aatggcaacc agggcgacga cttccttacc
     2401 ccctctgggc tggcggagcc ccgggtggag gacttcggga acgcctggaa gctgcacggg
     2461 gactgccagg acctgcagaa gcagcacagc gatccctgcg ccctcaaccc gcgcatgacc
     2521 aggttctccg aggaggcgtg cgcggtcctg acgtccccca cattcgaggc ctgccatcgt
     2581 gccgtcagcc cgctgcccta cctgcggaac tgccgctacg acgtgtgctc ctgctcggac
     2641 ggccgcgagt gcctgtgcgg cgccctggcc agctatgccg cggcctgcgc ggggagaggc
     2701 gtgcgcgtcg cgtggcgcga gccaggccgc tgtgagctga actgcccgaa aggccaggtg
     2761 tacctgcagt gcgggacccc ctgcaacctg acctgccgct ctctctctta cccggatgag
     2821 gaatgcaatg aggcctgcct ggagggctgc ttctgccccc cagggctcta catggatgag
     2881 aggggggact gcgtgcccaa ggcccagtgc ccctgttact atgacggtga gatcttccag
     2941 ccagaagaca tcttctcaga ccatcacacc atgtgctact gtgaggatgg cttcatgcac
     3001 tgtaccatga gtggagtccc cggaagcttg ctgcctgacg ctgtcctcag cagtcccctg
     3061 tctcatcgca gcaaaaggag cctatcctgt cggcccccca tggtcaagct ggtgtgtccc
     3121 gctgacaacc tgcgggctga agggctcgag tgtaccaaaa cgtgccagaa ctatgacctg
     3181 gagtgcatga gcatgggctg tgtctctggc tgcctctgcc ccccgggcat ggtccggcat
     3241 gagaacagat gtgtggccct ggaaaggtgt ccctgcttcc atcagggcaa ggagtatgcc
     3301 cctggagaaa cagtgaagat tggctgcaac acttgtgtct gtcgggaccg gaagtggaac
     3361 tgcacagacc atgtgtgtga tgccacgtgc tccacgatcg gcatggccca ctacctcacc
     3421 ttcgacgggc tcaaatacct gttccccggg gagtgccagt acgttctggt gcaggattac
     3481 tgcggcagta accctgggac ctttcggatc ctagtgggga ataagggatg cagccacccc
     3541 tcagtgaaat gcaagaaacg ggtcaccatc ctggtggagg gaggagagat tgagctgttt
     3601 gacggggagg tgaatgtgaa gaggcccatg aaggatgaga ctcactttga ggtggtggag
     3661 tctggccggt acatcattct gctgctgggc aaagccctct ccgtggtctg ggaccgccac
     3721 ctgagcatct ccgtggtcct gaagcagaca taccaggaga aagtgtgtgg cctgtgtggg
     3781 aattttgatg gcatccagaa caatgacctc accagcagca acctccaagt ggaggaagac
     3841 cctgtggact ttgggaactc ctggaaagtg agctcgcagt gtgctgacac cagaaaagtg
     3901 cctctggact catcccctgc cacctgccat aacaacatca tgaagcagac gatggtggat
     3961 tcctcctgta gaatccttac cagtgacgtc ttccaggact gcaacaagct ggtggacccc
     4021 gagccatatc tggatgtctg catttacgac acctgctcct gtgagtccat tggggactgc
     4081 gcctgcttct gcgacaccat tgctgcctat gcccacgtgt gtgcccagca tggcaaggtg
     4141 gtgacctgga ggacggccac attgtgcccc cagagctgcg aggagaggaa tctccgggag
     4201 aacgggtatg agtgtgagtg gcgctataac agctgtgcac ctgcctgtca agtcacgtgt
     4261 cagcaccctg agccactggc ctgccctgtg cagtgtgtgg agggctgcca tgcccactgc
     4321 cctccaggga aaatcctgga tgagcttttg cagacctgcg ttgaccctga agactgtcca
     4381 gtgtgtgagg tggctggccg gcgttttgcc tcaggaaaga aagtcacctt gaatcccagt
     4441 gaccctgagc actgccagat ttgccactgt gatgttgtca acctcacctg tgaagcctgc
     4501 caggagccgg gaggcctggt ggtgcctccc acagatgccc cggtgagccc caccactctg
     4561 tatgtggagg acatcttcga acccccccac atggcacaag ttaacgtagg tccggggctc
     4621 ttgggggttt cgaccctggg gcccaagagg aactccatgg ttctggctgt ggcgttcgtc
     4681 ctggaaggat cggacaaaat tggtgaagcc gacttcaaca ggagcaagga gttcatggag
     4741 gaggtgattc agcggatgga tgtgggccag gacagcatcc acgtcacggt gctgcagtac
     4801 tcctacatgg tgaccgtgga gtaccccttc agcgaggcac agtccaaagg ggacatcctg
     4861 cagcgggtgc gagagatccg ctaccagggc ggcaacagga ccaacactgg gctggccctg
     4921 cggtacctct ctgaccacag cttcttggtc agccagggtg accgggagca ggcgcccaac
     4981 ctggtctaca tggtcaccgg aaatcctgcc tctgatgaga tcaagaggct gcctggagac
     5041 atccaggtgg tgcccattgg agtgggccct aatgccaacg tgcaggagct ggagaggatt
     5101 ggctggccca atgcccctat cctcatccag gactttgaga cgctcccccg agaggctcct
     5161 gacctggtgc tgcagagttg ttgtaccggt gaggggctgc agatccccac cctctcccct
     5221 gcacctgact gcagccagcc cctggacgtg atccttctcc tggatggctc ctccagtttc
     5281 ccagcttctt attttgatga aatgaagagt ttcgccaagg ctttcatttc aaaagccaat
     5341 atagggcctc gtctcactca ggtgtcagtg ctgcagtatg gaagcatcac caccattgac
     5401 gtgccatgga acgtggtccc ggagaaagcc catttgctga gccttgtgga cgtcatgcag
     5461 cgggagggag gccccagcca aatcggggat gccttgggct ttgctgtgcg atacttgact
     5521 tcagaaatgc atggtgccag gccgggagcc tcaaaggcgg tggtcatcct ggtcacggac
     5581 gtctctgtgg attcagtgga tgcagcagct gatgccgcca ggtccaacag agtgacagtg
     5641 ttccctattg gaattggaga tcgctacgat gcagcccagc tacggatctt ggcaggccca
     5701 gcaggcgact ccaacgtggt gaagctccag cgaatcgaag acctccctac catggtcacc
     5761 ttgggcaatt ccttcctcca caaactgtgc tctggatttg ttaggatttg catggatgag
     5821 gatgggaatg agaagaggcc cggggacgtc tggaccttgc cagaccagtg ccacaccgtg
     5881 acttgccagc cagatggcca gaccttgctg aagagtcatc gggtcaactg tgaccggggg
     5941 ctgaggcctt cgtgccctaa cagccagtcc cctgttaaag tggaagagac ctgtggctgc
     6001 cgctggacct gcccctgcgt gtgcacaggc agctccactc ggcacatcgt gacctttgat
     6061 gggcagaatt tcaagctgac tggcagctgt tcttatgtcc tatttcaaaa caaggagcag
     6121 gacctggagg tgattctcca taatggtgcc tgcagccctg gagcaaggca gggctgcatg
     6181 aaatccatcg aggtgaagca cagtgccctc tccgtcgagc tgcacagtga catggaggtg
     6241 acggtgaatg ggagactggt ctctgttcct tacgtgggtg ggaacatgga agtcaacgtt
     6301 tatggtgcca tcatgcatga ggtcagattc aatcaccttg gtcacatctt cacattcact
     6361 ccacaaaaca atgagttcca actgcagctc agccccaaga cttttgcttc aaagacgtat
     6421 ggtctgtgtg ggatctgtga tgagaacgga gccaatgact tcatgctgag ggatggcaca
     6481 gtcaccacag actggaaaac acttgttcag gaatggactg tgcagcggcc agggcagacg
     6541 tgccagccca tcctggagga gcagtgtctt gtccccgaca gctcccactg ccaggtcctc
     6601 ctcttaccac tgtttgctga atgccacaag gtcctggctc cagccacatt ctatgccatc
     6661 tgccagcagg acagttgcca ccaggagcaa gtgtgtgagg tgatcgcctc ttatgcccac
     6721 ctctgtcgga ccaacggggt ctgcgttgac tggaggacac ctgatttctg tgctatgtca
     6781 tgcccaccat ctctggtcta caaccactgt gagcatggct gtccccggca ctgtgatggc
     6841 aacgtgagct cctgtgggga ccatccctcc gaaggctgtt tctgccctcc agataaagtc
     6901 atgttggaag gcagctgtgt ccctgaagag gcctgcactc agtgcattgg tgaggatgga
     6961 gtccagcacc agttcctgga agcctgggtc ccggaccacc agccctgtca gatctgcaca
     7021 tgcctcagcg ggcggaaggt caactgcaca acgcagccct gccccacggc caaagctccc
     7081 acgtgtggcc tgtgtgaagt agcccgcctc cgccagaatg cagaccagtg ctgccccgag
     7141 tatgagtgtg tgtgtgaccc agtgagctgt gacctgcccc cagtgcctca ctgtgaacgt
     7201 ggcctccagc ccacactgac caaccctggc gagtgcagac ccaacttcac ctgcgcctgc
     7261 aggaaggagg agtgcaaaag agtgtcccca ccctcctgcc ccccgcaccg tttgcccacc
     7321 cttcggaaga cccagtgctg tgatgagtat gagtgtgcct gcaactgtgt caactccaca
     7381 gtgagctgtc cccttgggta cttggcctca accgccacca atgactgtgg ctgtaccaca
     7441 accacctgcc ttcccgacaa ggtgtgtgtc caccgaagca ccatctaccc tgtgggccag
     7501 ttctgggagg agggctgcga tgtgtgcacc tgcaccgaca tggaggatgc cgtgatgggc
     7561 ctccgcgtgg cccagtgctc ccagaagccc tgtgaggaca gctgtcggtc gggcttcact
     7621 tacgttctgc atgaaggcga gtgctgtgga aggtgcctgc catctgcctg tgaggtggtg
     7681 actggctcac cgcgggggga ctcccagtct tcctggaaga gtgtcggctc ccagtgggcc
     7741 tccccggaga acccctgcct catcaatgag tgtgtccgag tgaaggagga ggtctttata
     7801 caacaaagga acgtctcctg cccccagctg gaggtccctg tctgcccctc gggctttcag
     7861 ctgagctgta agacctcagc gtgctgccca agctgtcgct gtgagcgcat ggaggcctgc
     7921 atgctcaatg gcactgtcat tgggcccggg aagactgtga tgatcgatgt gtgcacgacc
     7981 tgccgctgca tggtgcaggt gggggtcatc tctggattca agctggagtg caggaagacc
     8041 acctgcaacc cctgccccct gggttacaag gaagaaaata acacaggtga atgttgtggg
     8101 agatgtttgc ctacggcttg caccattcag ctaagaggag gacagatcat gacactgaag
     8161 cgtgatgaga cgctccagga tggctgtgat actcacttct gcaaggtcaa tgagagagga
     8221 gagtacttct gggagaagag ggtcacaggc tgcccaccct ttgatgaaca caagtgtctg
     8281 gctgagggag gtaaaattat gaaaattcca ggcacctgct gtgacacatg tgaggagcct
     8341 gagtgcaacg acatcactgc caggctgcag tatgtcaagg tgggaagctg taagtctgaa
     8401 gtagaggtgg atattcacta ctgccagggc aaatgtgcca gcaaagccat gtactccatt
     8461 gacatcaacg atgtgcagga ccagtgctcc tgctgctctc cgacacggac ggagcccatg
     8521 caggtggccc tgcactgcac caatggctct gttgtgtacc atgaggttct caatgccatg
     8581 gagtgcaaat gctcccccag gaagtgcagc aaggatatcg ctagcgaaca gaagctaata
     8641 agtgaagagg acctaatctg agaattcaaa cccgctgatc agcctcgact gtgccttcta
     8701 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     8761 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     8821 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     8881 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     8941 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     9001 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     9061 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     9121 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     9181 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     9241 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     9301 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     9361 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     9421 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9481 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9541 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     9601 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9661 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9721 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgat
     9781 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     9841 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     9901 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     9961 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
    10021 ggagttcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
    10081 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
    10141 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
    10201 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
    10261 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
    10321 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
    10381 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
    10441 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
    10501 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
    10561 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
    10621 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
    10681 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
    10741 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
    10801 atagcacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    10861 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    10921 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    10981 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    11041 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
    11101 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    11161 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    11221 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    11281 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    11341 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    11401 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    11461 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    11521 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    11581 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    11641 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    11701 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    11761 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    11821 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    11881 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    11941 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    12001 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    12061 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    12121 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    12181 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    12241 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    12301 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    12361 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    12421 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    12481 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    12541 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    12601 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    12661 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    12721 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    12781 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    12841 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    12901 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    12961 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    13021 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    13081 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    13141 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    13201 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    13261 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
    13321 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
    13381 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
//
