LOCUS       sequence-243925-       13440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses multimeric human VWF protein without A1 domain and Lock A2
            domain. This protein has C-terminal c-Myc tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13440)
  AUTHORS   Zhang C, Kelkar A, Neelamegham S
  TITLE     von Willebrand factor self-association is regulated by the 
            shear-dependent unfolding of the A2 domain.
  JOURNAL   Blood Adv. 2019 Apr 9;3(7):957-968. doi: 
            10.1182/bloodadvances.2018030122.
  PUBMED    30936056
REFERENCE   2  (bases 1 to 13440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/bloodadvances"; journalName: "Blood Adv"; date: "2019-04-9-
            9"; volume: "3"; issue: "7"; pages: "957-968"
COMMENT     Sequence Label: pcDNA3.1-deltaA1-Lock-VWF
FEATURES             Location/Qualifiers
     source          1..13440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..445
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        446..649
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     600..620
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     694..713
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        694..712
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2371..2382
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             8636..8665
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(8693..8710)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8699..8923
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8969..9397
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9056..9075)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9266..9287
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9406..9426)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9411..9740
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9591..9726
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9653..9672
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9789..10814
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    10944..11065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10981..11000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11035..11054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11114..11130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11114..11130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11127..11149)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11138..11154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11162..11168,11169..11186,11187..11192))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11207..11228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11345..11362)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11516..12104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11596..11615)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12275..13066,13067..13135))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12898..12917
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13136..13240)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13315..13334)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
       61 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      121 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      181 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      241 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      301 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      361 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      421 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      481 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      541 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      601 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
      661 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
      721 agctggctag cgtttaaact taagctgatc cactagtcca gtgtggtgga attcccgcag
      781 ccctcatttg caggggaaga tgattcctgc cagatttgcc ggggtgctgc ttgctctggc
      841 cctcattttg ccagggaccc tttgtgcaga aggaactcgc ggcaggtcat ccacggcccg
      901 atgcagcctt ttcggaagtg acttcgtcaa cacctttgat gggagcatgt acagctttgc
      961 gggatactgc agttacctcc tggcaggggg ctgccagaaa cgctccttct cgattattgg
     1021 ggacttccag aatggcaaga gagtgagcct ctccgtgtat cttggggaat tttttgacat
     1081 ccatttgttt gtcaatggta ccgtgacaca gggggaccaa agagtctcca tgccctatgc
     1141 ctccaaaggg ctgtatctag aaactgaggc tgggtactac aagctgtccg gtgaggccta
     1201 tggctttgtg gccaggatcg atggcagcgg caactttcaa gtcctgctgt cagacagata
     1261 cttcaacaag acctgcgggc tgtgtggcaa ctttaacatc tttgctgaag atgactttat
     1321 gacccaagaa gggaccttga cctcggaccc ttatgacttt gccaactcat gggctctgag
     1381 cagtggagaa cagtggtgtg aacgggcatc tcctcccagc agctcatgca acatctcctc
     1441 tggggaaatg cagaagggcc tgtgggagca gtgccagctt ctgaagagca cctcggtgtt
     1501 tgcccgctgc caccctctgg tggaccccga gccttttgtg gccctgtgtg agaagacttt
     1561 gtgtgagtgt gctggggggc tggagtgcgc ctgccctgcc ctcctggagt acgcccggac
     1621 ctgtgcccag gagggaatgg tgctgtacgg ctggaccgac cacagcgcgt gcagcccagt
     1681 gtgccctgct ggtatggagt ataggcagtg tgtgtcccct tgcgccagga cctgccagag
     1741 cctgcacatc aatgaaatgt gtcaggagcg atgcgtggat ggctgcagct gccctgaggg
     1801 acagctcctg gatgaaggcc tctgcgtgga gagcaccgag tgtccctgcg tgcattccgg
     1861 aaagcgctac cctcccggca cctccctctc tcgagactgc aacacctgca tttgccgaaa
     1921 cagccagtgg atctgcagca atgaagaatg tccaggggag tgccttgtca caggtcaatc
     1981 acacttcaag agctttgaca acagatactt caccttcagt gggatctgcc agtacctgct
     2041 ggcccgggat tgccaggacc actccttctc cattgtcatt gagactgtcc agtgtgctga
     2101 tgaccgcgac gctgtgtgca cccgctccgt caccgtccgg ctgcctggcc tgcacaacag
     2161 ccttgtgaaa ctgaagcatg gggcaggagt tgccatggat ggccaggacg tccagctccc
     2221 cctcctgaaa ggtgacctcc gcatccagca tacagtgacg gcctccgtgc gcctcagcta
     2281 cggggaggac ctgcagatgg actgggatgg ccgcgggagg ctgctggtga agctgtcccc
     2341 cgtctatgcc gggaagacct gcggcctgtg tgggaattac aatggcaacc agggcgacga
     2401 cttccttacc ccctctgggc tggcggagcc ccgggtggag gacttcggga acgcctggaa
     2461 gctgcacggg gactgccagg acctgcagaa gcagcacagc gatccctgcg ccctcaaccc
     2521 gcgcatgacc aggttctccg aggaggcgtg cgcggtcctg acgtccccca cattcgaggc
     2581 ctgccatcgt gccgtcagcc cgctgcccta cctgcggaac tgccgctacg acgtgtgctc
     2641 ctgctcggac ggccgcgagt gcctgtgcgg cgccctggcc agctatgccg cggcctgcgc
     2701 ggggagaggc gtgcgcgtcg cgtggcgcga gccaggccgc tgtgagctga actgcccgaa
     2761 aggccaggtg tacctgcagt gcgggacccc ctgcaacctg acctgccgct ctctctctta
     2821 cccggatgag gaatgcaatg aggcctgcct ggagggctgc ttctgccccc cagggctcta
     2881 catggatgag aggggggact gcgtgcccaa ggcccagtgc ccctgttact atgacggtga
     2941 gatcttccag ccagaagaca tcttctcaga ccatcacacc atgtgctact gtgaggatgg
     3001 cttcatgcac tgtaccatga gtggagtccc cggaagcttg ctgcctgacg ctgtcctcag
     3061 cagtcccctg tctcatcgca gcaaaaggag cctatcctgt cggcccccca tggtcaagct
     3121 ggtgtgtccc gctgacaacc tgcgggctga agggctcgag tgtaccaaaa cgtgccagaa
     3181 ctatgacctg gagtgcatga gcatgggctg tgtctctggc tgcctctgcc ccccgggcat
     3241 ggtccggcat gagaacagat gtgtggccct ggaaaggtgt ccctgcttcc atcagggcaa
     3301 ggagtatgcc cctggagaaa cagtgaagat tggctgcaac acttgtgtct gtcgggaccg
     3361 gaagtggaac tgcacagacc atgtgtgtga tgccacgtgc tccacgatcg gcatggccca
     3421 ctacctcacc ttcgacgggc tcaaatacct gttccccggg gagtgccagt acgttctggt
     3481 gcaggattac tgcggcagta accctgggac ctttcggatc ctagtgggga ataagggatg
     3541 cagccacccc tcagtgaaat gcaagaaacg ggtcaccatc ctggtggagg gaggagagat
     3601 tgagctgttt gacggggagg tgaatgtgaa gaggcccatg aaggatgaga ctcactttga
     3661 ggtggtggag tctggccggt acatcattct gctgctgggc aaagccctct ccgtggtctg
     3721 ggaccgccac ctgagcatct ccgtggtcct gaagcagaca taccaggaga aagtgtgtgg
     3781 cctgtgtggg aattttgatg gcatccagaa caatgacctc accagcagca acctccaagt
     3841 ggaggaagac cctgtggact ttgggaactc ctggaaagtg agctcgcagt gtgctgacac
     3901 cagaaaagtg cctctggact catcccctgc cacctgccat aacaacatca tgaagcagac
     3961 gatggtggat tcctcctgta gaatccttac cagtgacgtc ttccaggact gcaacaagct
     4021 ggtggacccc gagccatatc tggatgtctg catttacgac acctgctcct gtgagtccat
     4081 tggggactgc gcctgcttct gcgacaccat tgctgcctat gcccacgtgt gtgcccagca
     4141 tggcaaggtg gtgacctgga ggacggccac attgtgcccc cagagctgcg aggagaggaa
     4201 tctccgggag aacgggtatg agtgtgagtg gcgctataac agctgtgcac ctgcctgtca
     4261 agtcacgtgt cagcaccctg agccactggc ctgccctgtg cagtgtgtgg agggctgcca
     4321 tgcccactgc cctccaggga aaatcctgga tgagcttttg cagacctgcg ttgaccctga
     4381 agactgtcca gtgtgtgagg tggctggccg gcgttttgcc tcaggaaaga aagtcacctt
     4441 gaatcccagt gaccctgagc actgccagat ttgccactgt gatgttgtca acctcacctg
     4501 tgaagcctgc caggagccgg gaggcctggt ggtgcctccc acagatgccc cggtgagccc
     4561 caccactctg tatgtggagg acatcttcga acccccccac atggcacaag ttaacgtagg
     4621 tccggggctc ttgggggttt cgaccctggg gcccaagagg tgctccatgg ttctggatgt
     4681 ggcgttcgtc ctggaaggat cggacaaaat tggtgaagcc gacttcaaca ggagcaagga
     4741 gttcatggag gaggtgattc agcggatgga tgtgggccag gacagcatcc acgtcacggt
     4801 gctgcagtac tcctacatgg tgaccgtgga gtaccccttc agcgaggcac agtccaaagg
     4861 ggacatcctg cagcgggtgc gagagatccg ctaccagggc ggcaacagga ccaacactgg
     4921 gctggccctg cggtacctct ctgaccacag cttcttggtc agccagggtg accgggagca
     4981 ggcgcccaac ctggtctaca tggtcaccgg aaatcctgcc tctgatgaga tcaagaggct
     5041 gcctggagac atccaggtgg tgcccattgg agtgggccct aatgccaacg tgcaggagct
     5101 ggagaggatt ggctggccca atgcccctat cctcatccag gactttgaga cgctcccccg
     5161 agaggctcct gacctggtgc tgcagagtgg ttgtaccggt gaggggctgc agatccccac
     5221 cctctcccct gcacctgact gcagccagcc cctggacgtg atccttctcc tggatggctc
     5281 ctccagtttc ccagcttctt attttgatga aatgaagagt ttcgccaagg ctttcatttc
     5341 aaaagccaat atagggcctc gtctcactca ggtgtcagtg ctgcagtatg gaagcatcac
     5401 caccattgac gtgccatgga acgtggtccc ggagaaagcc catttgctga gccttgtgga
     5461 cgtcatgcag cgggagggag gccccagcca aatcggggat gccttgggct ttgctgtgcg
     5521 atacttgact tcagaaatgc atggtgccag gccgggagcc tcaaaggcgg tggtcatcct
     5581 ggtcacggac gtctctgtgg attcagtgga tgcagcagct gatgccgcca ggtccaacag
     5641 agtgacagtg ttccctattg gaattggaga tcgctacgat gcagcccagc tacggatctt
     5701 ggcaggccca gcaggcgact ccaacgtggt gaagctccag cgaatcgaag acctccctac
     5761 catggtcacc ttgggcaatt ccttcctcca caaactgtgc tctggatttg ttaggatttg
     5821 catggatgag gatgggaatg agaagaggcc cggggacgtc tggaccttgc cagaccagtg
     5881 ccacaccgtg acttgccagc cagatggcca gaccttgctg aagagtcatc gggtcaactg
     5941 tgaccggggg ctgaggcctt cgtgccctaa cagccagtcc cctgttaaag tggaagagac
     6001 ctgtggctgc cgctggacct gcccctgcgt gtgcacaggc agctccactc ggcacatcgt
     6061 gacctttgat gggcagaatt tcaagctgac tggcagctgt tcttatgtcc tatttcaaaa
     6121 caaggagcag gacctggagg tgattctcca taatggtgcc tgcagccctg gagcaaggca
     6181 gggctgcatg aaatccatcg aggtgaagca cagtgccctc tccgtcgagc tgcacagtga
     6241 catggaggtg acggtgaatg ggagactggt ctctgttcct tacgtgggtg ggaacatgga
     6301 agtcaacgtt tatggtgcca tcatgcatga ggtcagattc aatcaccttg gtcacatctt
     6361 cacattcact ccacaaaaca atgagttcca actgcagctc agccccaaga cttttgcttc
     6421 aaagacgtat ggtctgtgtg ggatctgtga tgagaacgga gccaatgact tcatgctgag
     6481 ggatggcaca gtcaccacag actggaaaac acttgttcag gaatggactg tgcagcggcc
     6541 agggcagacg tgccagccca tcctggagga gcagtgtctt gtccccgaca gctcccactg
     6601 ccaggtcctc ctcttaccac tgtttgctga atgccacaag gtcctggctc cagccacatt
     6661 ctatgccatc tgccagcagg acagttgcca ccaggagcaa gtgtgtgagg tgatcgcctc
     6721 ttatgcccac ctctgtcgga ccaacggggt ctgcgttgac tggaggacac ctgatttctg
     6781 tgctatgtca tgcccaccat ctctggtcta caaccactgt gagcatggct gtccccggca
     6841 ctgtgatggc aacgtgagct cctgtgggga ccatccctcc gaaggctgtt tctgccctcc
     6901 agataaagtc atgttggaag gcagctgtgt ccctgaagag gcctgcactc agtgcattgg
     6961 tgaggatgga gtccagcacc agttcctgga agcctgggtc ccggaccacc agccctgtca
     7021 gatctgcaca tgcctcagcg ggcggaaggt caactgcaca acgcagccct gccccacggc
     7081 caaagctccc acgtgtggcc tgtgtgaagt agcccgcctc cgccagaatg cagaccagtg
     7141 ctgccccgag tatgagtgtg tgtgtgaccc agtgagctgt gacctgcccc cagtgcctca
     7201 ctgtgaacgt ggcctccagc ccacactgac caaccctggc gagtgcagac ccaacttcac
     7261 ctgcgcctgc aggaaggagg agtgcaaaag agtgtcccca ccctcctgcc ccccgcaccg
     7321 tttgcccacc cttcggaaga cccagtgctg tgatgagtat gagtgtgcct gcaactgtgt
     7381 caactccaca gtgagctgtc cccttgggta cttggcctca accgccacca atgactgtgg
     7441 ctgtaccaca accacctgcc ttcccgacaa ggtgtgtgtc caccgaagca ccatctaccc
     7501 tgtgggccag ttctgggagg agggctgcga tgtgtgcacc tgcaccgaca tggaggatgc
     7561 cgtgatgggc ctccgcgtgg cccagtgctc ccagaagccc tgtgaggaca gctgtcggtc
     7621 gggcttcact tacgttctgc atgaaggcga gtgctgtgga aggtgcctgc catctgcctg
     7681 tgaggtggtg actggctcac cgcgggggga ctcccagtct tcctggaaga gtgtcggctc
     7741 ccagtgggcc tccccggaga acccctgcct catcaatgag tgtgtccgag tgaaggagga
     7801 ggtctttata caacaaagga acgtctcctg cccccagctg gaggtccctg tctgcccctc
     7861 gggctttcag ctgagctgta agacctcagc gtgctgccca agctgtcgct gtgagcgcat
     7921 ggaggcctgc atgctcaatg gcactgtcat tgggcccggg aagactgtga tgatcgatgt
     7981 gtgcacgacc tgccgctgca tggtgcaggt gggggtcatc tctggattca agctggagtg
     8041 caggaagacc acctgcaacc cctgccccct gggttacaag gaagaaaata acacaggtga
     8101 atgttgtggg agatgtttgc ctacggcttg caccattcag ctaagaggag gacagatcat
     8161 gacactgaag cgtgatgaga cgctccagga tggctgtgat actcacttct gcaaggtcaa
     8221 tgagagagga gagtacttct gggagaagag ggtcacaggc tgcccaccct ttgatgaaca
     8281 caagtgtctg gctgagggag gtaaaattat gaaaattcca ggcacctgct gtgacacatg
     8341 tgaggagcct gagtgcaacg acatcactgc caggctgcag tatgtcaagg tgggaagctg
     8401 taagtctgaa gtagaggtgg atattcacta ctgccagggc aaatgtgcca gcaaagccat
     8461 gtactccatt gacatcaacg atgtgcagga ccagtgctcc tgctgctctc cgacacggac
     8521 ggagcccatg caggtggccc tgcactgcac caatggctct gttgtgtacc atgaggttct
     8581 caatgccatg gagtgcaaat gctcccccag gaagtgcagc aaggatatcg ctagcgaaca
     8641 gaagctaata agtgaagagg acctaatctg agaattcaaa cccgctgatc agcctcgact
     8701 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     8761 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     8821 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     8881 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     8941 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     9001 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9061 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9121 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9181 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9241 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9301 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9361 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     9421 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9481 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     9541 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     9601 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9661 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9721 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     9781 agcacgtgat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     9841 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     9901 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     9961 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
    10021 ttgacattgg ggagttcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
    10081 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
    10141 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
    10201 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
    10261 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
    10321 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
    10381 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
    10441 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
    10501 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
    10561 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
    10621 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
    10681 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
    10741 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
    10801 gggcaaagga atagcacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
    10861 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
    10921 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
    10981 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    11041 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
    11101 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    11161 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    11221 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    11281 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    11341 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    11401 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    11461 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    11521 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    11581 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    11641 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    11701 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    11761 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    11821 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    11881 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    11941 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    12001 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    12061 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    12121 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    12181 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    12241 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    12301 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    12361 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    12421 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    12481 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    12541 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    12601 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    12661 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    12721 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    12781 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    12841 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    12901 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    12961 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    13021 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    13081 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    13141 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    13201 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    13261 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
    13321 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
    13381 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
//
