LOCUS       sequence-243927-       17069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses multimeric human VWF-ECFP fusion protein with A2-Lock 
            domain The cyan fluorescence protein coding region is inserted 
            between the VWF-D'D3 and A1 domains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17069)
  AUTHORS   Zhang C, Kelkar A, Neelamegham S
  TITLE     von Willebrand factor self-association is regulated by the 
            shear-dependent unfolding of the A2 domain.
  JOURNAL   Blood Adv. 2019 Apr 9;3(7):957-968. doi: 
            10.1182/bloodadvances.2018030122.
  PUBMED    30936056
REFERENCE   2  (bases 1 to 17069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/bloodadvances"; journalName: "Blood Adv"; date: "2019-04-9-
            9"; volume: "3"; issue: "7"; pages: "957-968"
COMMENT     Sequence Label: pCS-CG-Lock-Cer-VWF
FEATURES             Location/Qualifiers
     source          1..17069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        70..449
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        451..649
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             667..847
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    894..1019
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1512..1745
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1930..1974
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2298..2601
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2602..2805
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4463..4474
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             join(6684..6686,6687..6689,6690..7202)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(6729..6750)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6990..7009)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7337..7358
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             12155..12175
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             12176..12193
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     LTR             12706..12886
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12912..12929)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12918..13142
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      13188..13616
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13275..13294)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13485..13506
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13625..13645)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13630..13959
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13810..13945
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13872..13891
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        14007..14054
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             14073..14447
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    14577..14698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14614..14633)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14668..14687
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(14747..14763)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14747..14763)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14760..14782)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14771..14787
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14795..14801,14802..14819,14820..14825))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14840..14861
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14978..14995)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15149..15737)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15229..15248)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15908..16699,16700..16768))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16531..16550
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16769..16873)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(16948..16967)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
       61 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      121 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      181 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      241 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      301 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      361 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      421 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      481 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      541 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      601 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc
      661 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
      721 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
      781 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
      841 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
      901 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
      961 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     1021 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     1081 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     1141 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     1201 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     1261 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     1321 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     1381 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     1441 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     1501 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     1561 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     1621 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     1681 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     1741 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     1801 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     1861 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     1921 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     1981 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     2041 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     2101 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     2161 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     2221 tcccccgggc tgcaggaatt cgattaatag taatcaatta cggggtcatt agttcatagc
     2281 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     2341 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2401 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2461 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2521 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2581 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2641 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2701 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2761 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2821 cagatccgct agcaggcgcg ccagtcgact cccgggatcc caattcccgc agccctcatt
     2881 tgcaggggaa gatgattcct gccagatttg ccggggtgct gcttgctctg gccctcattt
     2941 tgccagggac cctttgtgca gaaggaactc gcggcaggtc atccacggcc cgatgcagcc
     3001 ttttcggaag tgacttcgtc aacacctttg atgggagcat gtacagcttt gcgggatact
     3061 gcagttacct cctggcaggg ggctgccaga aacgctcctt ctcgattatt ggggacttcc
     3121 agaatggcaa gagagtgagc ctctccgtgt atcttgggga attttttgac atccatttgt
     3181 ttgtcaatgg taccgtgaca cagggggacc aaagagtctc catgccctat gcctccaaag
     3241 ggctgtatct agaaactgag gctgggtact acaagctgtc cggtgaggcc tatggctttg
     3301 tggccaggat cgatggcagc ggcaactttc aagtcctgct gtcagacaga tacttcaaca
     3361 agacctgcgg gctgtgtggc aactttaaca tctttgctga agatgacttt atgacccaag
     3421 aagggacctt gacctcggac ccttatgact ttgccaactc atgggctctg agcagtggag
     3481 aacagtggtg tgaacgggca tctcctccca gcagctcatg caacatctcc tctggggaaa
     3541 tgcagaaggg cctgtgggag cagtgccagc ttctgaagag cacctcggtg tttgcccgct
     3601 gccaccctct ggtggacccc gagccttttg tggccctgtg tgagaagact ttgtgtgagt
     3661 gtgctggggg gctggagtgc gcctgccctg ccctcctgga gtacgcccgg acctgtgccc
     3721 aggagggaat ggtgctgtac ggctggaccg accacagcgc gtgcagccca gtgtgccctg
     3781 ctggtatgga gtataggcag tgtgtgtccc cttgcgccag gacctgccag agcctgcaca
     3841 tcaatgaaat gtgtcaggag cgatgcgtgg atggctgcag ctgccctgag ggacagctcc
     3901 tggatgaagg cctctgcgtg gagagcaccg agtgtccctg cgtgcattcc ggaaagcgct
     3961 accctcccgg cacctccctc tctcgagact gcaacacctg catttgccga aacagccagt
     4021 ggatctgcag caatgaagaa tgtccagggg agtgccttgt cacaggtcaa tcacacttca
     4081 agagctttga caacagatac ttcaccttca gtgggatctg ccagtacctg ctggcccggg
     4141 attgccagga ccactccttc tccattgtca ttgagactgt ccagtgtgct gatgaccgcg
     4201 acgctgtgtg cacccgctcc gtcaccgtcc ggctgcctgg cctgcacaac agccttgtga
     4261 aactgaagca tggggcagga gttgccatgg atggccagga cgtccagctc cccctcctga
     4321 aaggtgacct ccgcatccag catacagtga cggcctccgt gcgcctcagc tacggggagg
     4381 acctgcagat ggactgggat ggccgcggga ggctgctggt gaagctgtcc cccgtctatg
     4441 ccgggaagac ctgcggcctg tgtgggaatt acaatggcaa ccagggcgac gacttcctta
     4501 ccccctctgg gctggcggag ccccgggtgg aggacttcgg gaacgcctgg aagctgcacg
     4561 gggactgcca ggacctgcag aagcagcaca gcgatccctg cgccctcaac ccgcgcatga
     4621 ccaggttctc cgaggaggcg tgcgcggtcc tgacgtcccc cacattcgag gcctgccatc
     4681 gtgccgtcag cccgctgccc tacctgcgga actgccgcta cgacgtgtgc tcctgctcgg
     4741 acggccgcga gtgcctgtgc ggcgccctgg ccagctatgc cgcggcctgc gcggggagag
     4801 gcgtgcgcgt cgcgtggcgc gagccaggcc gctgtgagct gaactgcccg aaaggccagg
     4861 tgtacctgca gtgcgggacc ccctgcaacc tgacctgccg ctctctctct tacccggatg
     4921 aggaatgcaa tgaggcctgc ctggagggct gcttctgccc cccagggctc tacatggatg
     4981 agagggggga ctgcgtgccc aaggcccagt gcccctgtta ctatgacggt gagatcttcc
     5041 agccagaaga catcttctca gaccatcaca ccatgtgcta ctgtgaggat ggcttcatgc
     5101 actgtaccat gagtggagtc cccggaagct tgctgcctga cgctgtcctc agcagtcccc
     5161 tgtctcatcg cagcaaaagg agcctatcct gtcggccccc catggtcaag ctggtgtgtc
     5221 ccgctgacaa cctgcgggct gaagggctcg agtgtaccaa aacgtgccag aactatgacc
     5281 tggagtgcat gagcatgggc tgtgtctctg gctgcctctg ccccccgggc atggtccggc
     5341 atgagaacag atgtgtggcc ctggaaaggt gtccctgctt ccatcagggc aaggagtatg
     5401 cccctggaga aacagtgaag attggctgca acacttgtgt ctgtcgggac cggaagtgga
     5461 actgcacaga ccatgtgtgt gatgccacgt gctccacgat cggcatggcc cactacctca
     5521 ccttcgacgg gctcaaatac ctgttccccg gggagtgcca gtacgttctg gtgcaggatt
     5581 actgcggcag taaccctggg acctttcgga tcctagtggg gaataaggga tgcagccacc
     5641 cctcagtgaa atgcaagaaa cgggtcacca tcctggtgga gggaggagag attgagctgt
     5701 ttgacgggga ggtgaatgtg aagaggccca tgaaggatga gactcacttt gaggtggtgg
     5761 agtctggccg gtacatcatt ctgctgctgg gcaaagccct ctccgtggtc tgggaccgcc
     5821 acctgagcat ctccgtggtc ctgaagcaga cataccagga gaaagtgtgt ggcctgtgtg
     5881 ggaattttga tggcatccag aacaatgacc tcaccagcag caacctccaa gtggaggaag
     5941 accctgtgga ctttgggaac tcctggaaag tgagctcgca gtgtgctgac accagaaaag
     6001 tgcctctgga ctcatcccct gccacctgcc ataacaacat catgaagcag acgatggtgg
     6061 attcctcctg tagaatcctt accagtgacg tcttccagga ctgcaacaag ctggtggacc
     6121 ccgagccata tctggatgtc tgcatttacg acacctgctc ctgtgagtcc attggggact
     6181 gcgcctgctt ctgcgacacc attgctgcct atgcccacgt gtgtgcccag catggcaagg
     6241 tggtgacctg gaggacggcc acattgtgcc cccagagctg cgaggagagg aatctccggg
     6301 agaacgggta tgagtgtgag tggcgctata acagctgtgc acctgcctgt caagtcacgt
     6361 gtcagcaccc tgagccactg gcctgccctg tgcagtgtgt ggagggctgc catgcccact
     6421 gccctccagg gaaaatcctg gatgagcttt tgcagacctg cgttgaccct gaagactgtc
     6481 cagtgtgtga ggtggctggc cggcgttttg cctcaggaaa gaaagtcacc ttgaatccca
     6541 gtgaccctga gcactgccag atttgccact gtgatgttgt caacctcacc tgtgaagcct
     6601 gccaggagcc gggaggcctg gtggtgcctc ccacagatgc cccggtgagc cccaccactc
     6661 tgtatgtgga ggacatcttc gaaatggtga gcaagggcga ggagctgttc accggggtgg
     6721 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     6781 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     6841 agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca
     6901 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     6961 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     7021 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     7081 aggacggcaa catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata
     7141 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     7201 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     7261 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     7321 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     7381 gcatggacga gttcgaaccg ccattgcacg atttctactg cagcaggcta ctggacctgg
     7441 tcttcctgct ggatggctcc tccaggctgt ccgaggctga gtttgaagtg ctgaaggcct
     7501 ttgtggtgga catgatggag cggctgcgca tctcccagaa gtgggtccgc gtggccgtgg
     7561 tggagtacca cgacggctcc cacgcctaca tcgggctcaa ggaccggaag cgaccgtcag
     7621 agctgcggcg cattgccagc caggtgaagt atgcgggcag ccaggtggcc tccaccagcg
     7681 aggtcttgaa atacacactg ttccaaatct tcagcaagat cgaccgccct gaagcctccc
     7741 gcatcgccct gctcctgatg gccagccagg agccccaacg gatgtcccgg aactttgtcc
     7801 gctacgtcca gggcctgaag aagaagaagg tcattgtgat cccggtgggc attgggcccc
     7861 atgccaacct caagcagatc cgcctcatcg agaagcaggc ccctgagaac aaggccttcg
     7921 tgctgagcag tgtggatgag ctggagcagc aaagggacga gatcgttagc tacctctgtg
     7981 accttgcccc tgaagcccct cctcctactc tgccccccca catggcacaa gttaacgtag
     8041 gtccggggct cttgggggtt tcgaccctgg ggcccaagag gtgctccatg gttctggatg
     8101 tggcgttcgt cctggaagga tcggacaaaa ttggtgaagc cgacttcaac aggagcaagg
     8161 agttcatgga ggaggtgatt cagcggatgg atgtgggcca ggacagcatc cacgtcacgg
     8221 tgctgcagta ctcctacatg gtgaccgtgg agtacccctt cagcgaggca cagtccaaag
     8281 gggacatcct gcagcgggtg cgagagatcc gctaccaggg cggcaacagg accaacactg
     8341 ggctggccct gcggtacctc tctgaccaca gcttcttggt cagccagggt gaccgggagc
     8401 aggcgcccaa cctggtctac atggtcaccg gaaatcctgc ctctgatgag atcaagaggc
     8461 tgcctggaga catccaggtg gtgcccattg gagtgggccc taatgccaac gtgcaggagc
     8521 tggagaggat tggctggccc aatgccccta tcctcatcca ggactttgag acgctccccc
     8581 gagaggctcc tgacctggtg ctgcagagtg gttgtaccgg tgaggggctg cagatcccca
     8641 ccctctcccc tgcacctgac tgcagccagc ccctggacgt gatccttctc ctggatggct
     8701 cctccagttt cccagcttct tattttgatg aaatgaagag tttcgccaag gctttcattt
     8761 caaaagccaa tatagggcct cgtctcactc aggtgtcagt gctgcagtat ggaagcatca
     8821 ccaccattga cgtgccatgg aacgtggtcc cggagaaagc ccatttgctg agccttgtgg
     8881 acgtcatgca gcgggaggga ggccccagcc aaatcgggga tgccttgggc tttgctgtgc
     8941 gatacttgac ttcagaaatg catggtgcca ggccgggagc ctcaaaggcg gtggtcatcc
     9001 tggtcacgga cgtctctgtg gattcagtgg atgcagcagc tgatgccgcc aggtccaaca
     9061 gagtgacagt gttccctatt ggaattggag atcgctacga tgcagcccag ctacggatct
     9121 tggcaggccc agcaggcgac tccaacgtgg tgaagctcca gcgaatcgaa gacctcccta
     9181 ccatggtcac cttgggcaat tccttcctcc acaaactgtg ctctggattt gttaggattt
     9241 gcatggatga ggatgggaat gagaagaggc ccggggacgt ctggaccttg ccagaccagt
     9301 gccacaccgt gacttgccag ccagatggcc agaccttgct gaagagtcat cgggtcaact
     9361 gtgaccgggg gctgaggcct tcgtgcccta acagccagtc ccctgttaaa gtggaagaga
     9421 cctgtggctg ccgctggacc tgcccctgcg tgtgcacagg cagctccact cggcacatcg
     9481 tgacctttga tgggcagaat ttcaagctga ctggcagctg ttcttatgtc ctatttcaaa
     9541 acaaggagca ggacctggag gtgattctcc ataatggtgc ctgcagccct ggagcaaggc
     9601 agggctgcat gaaatccatc gaggtgaagc acagtgccct ctccgtcgag ctgcacagtg
     9661 acatggaggt gacggtgaat gggagactgg tctctgttcc ttacgtgggt gggaacatgg
     9721 aagtcaacgt ttatggtgcc atcatgcatg aggtcagatt caatcacctt ggtcacatct
     9781 tcacattcac tccacaaaac aatgagttcc aactgcagct cagccccaag acttttgctt
     9841 caaagacgta tggtctgtgt gggatctgtg atgagaacgg agccaatgac ttcatgctga
     9901 gggatggcac agtcaccaca gactggaaaa cacttgttca ggaatggact gtgcagcggc
     9961 cagggcagac gtgccagccc atcctggagg agcagtgtct tgtccccgac agctcccact
    10021 gccaggtcct cctcttacca ctgtttgctg aatgccacaa ggtcctggct ccagccacat
    10081 tctatgccat ctgccagcag gacagttgcc accaggagca agtgtgtgag gtgatcgcct
    10141 cttatgccca cctctgtcgg accaacgggg tctgcgttga ctggaggaca cctgatttct
    10201 gtgctatgtc atgcccacca tctctggtct acaaccactg tgagcatggc tgtccccggc
    10261 actgtgatgg caacgtgagc tcctgtgggg accatccctc cgaaggctgt ttctgccctc
    10321 cagataaagt catgttggaa ggcagctgtg tccctgaaga ggcctgcact cagtgcattg
    10381 gtgaggatgg agtccagcac cagttcctgg aagcctgggt cccggaccac cagccctgtc
    10441 agatctgcac atgcctcagc gggcggaagg tcaactgcac aacgcagccc tgccccacgg
    10501 ccaaagctcc cacgtgtggc ctgtgtgaag tagcccgcct ccgccagaat gcagaccagt
    10561 gctgccccga gtatgagtgt gtgtgtgacc cagtgagctg tgacctgccc ccagtgcctc
    10621 actgtgaacg tggcctccag cccacactga ccaaccctgg cgagtgcaga cccaacttca
    10681 cctgcgcctg caggaaggag gagtgcaaaa gagtgtcccc accctcctgc cccccgcacc
    10741 gtttgcccac ccttcggaag acccagtgct gtgatgagta tgagtgtgcc tgcaactgtg
    10801 tcaactccac agtgagctgt ccccttgggt acttggcctc aaccgccacc aatgactgtg
    10861 gctgtaccac aaccacctgc cttcccgaca aggtgtgtgt ccaccgaagc accatctacc
    10921 ctgtgggcca gttctgggag gagggctgcg atgtgtgcac ctgcaccgac atggaggatg
    10981 ccgtgatggg cctccgcgtg gcccagtgct cccagaagcc ctgtgaggac agctgtcggt
    11041 cgggcttcac ttacgttctg catgaaggcg agtgctgtgg aaggtgcctg ccatctgcct
    11101 gtgaggtggt gactggctca ccgcgggggg actcccagtc ttcctggaag agtgtcggct
    11161 cccagtgggc ctccccggag aacccctgcc tcatcaatga gtgtgtccga gtgaaggagg
    11221 aggtctttat acaacaaagg aacgtctcct gcccccagct ggaggtccct gtctgcccct
    11281 cgggctttca gctgagctgt aagacctcag cgtgctgccc aagctgtcgc tgtgagcgca
    11341 tggaggcctg catgctcaat ggcactgtca ttgggcccgg gaagactgtg atgatcgatg
    11401 tgtgcacgac ctgccgctgc atggtgcagg tgggggtcat ctctggattc aagctggagt
    11461 gcaggaagac cacctgcaac ccctgccccc tgggttacaa ggaagaaaat aacacaggtg
    11521 aatgttgtgg gagatgtttg cctacggctt gcaccattca gctaagagga ggacagatca
    11581 tgacactgaa gcgtgatgag acgctccagg atggctgtga tactcacttc tgcaaggtca
    11641 atgagagagg agagtacttc tgggagaaga gggtcacagg ctgcccaccc tttgatgaac
    11701 acaagtgtct ggctgaggga ggtaaaatta tgaaaattcc aggcacctgc tgtgacacat
    11761 gtgaggagcc tgagtgcaac gacatcactg ccaggctgca gtatgtcaag gtgggaagct
    11821 gtaagtctga agtagaggtg gatatccact actgccaggg caaatgtgcc agcaaagcca
    11881 tgtactccat tgacatcaac gatgtgcagg accagtgctc ctgctgctct ccgacacgga
    11941 cggagcccat gcaggtggcc ctgcactgca ccaatggctc tgttgtgtac catgaggttc
    12001 tcaatgccat ggagtgcaaa tgctccccca ggaagtgcag caagtgagaa ttgctagcgc
    12061 cgccaccatg attcctgcca gatttgccgg ggtgctgctt gctctggccc tcattttgcc
    12121 agggaccctt tgtaccggtc cgcgggttaa cttcgaaaac ctgtacttcc agggccatca
    12181 ccatcaccat cactgactcg agacctagaa aaacatggag caatcacaag tagcaataca
    12241 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
    12301 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
    12361 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
    12421 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
    12481 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
    12541 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
    12601 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
    12661 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
    12721 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    12781 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    12841 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
    12901 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
    12961 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
    13021 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
    13081 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
    13141 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
    13201 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
    13261 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
    13321 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
    13381 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
    13441 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
    13501 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
    13561 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
    13621 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
    13681 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
    13741 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
    13801 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    13861 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
    13921 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
    13981 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
    14041 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
    14101 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
    14161 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
    14221 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
    14281 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
    14341 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
    14401 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
    14461 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
    14521 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
    14581 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    14641 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    14701 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
    14761 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    14821 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    14881 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    14941 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    15001 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    15061 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    15121 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    15181 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    15241 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    15301 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    15361 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    15421 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    15481 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    15541 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    15601 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    15661 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    15721 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    15781 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    15841 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    15901 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    15961 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    16021 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    16081 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    16141 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    16201 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    16261 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    16321 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    16381 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    16441 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    16501 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    16561 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    16621 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    16681 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    16741 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    16801 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    16861 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    16921 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    16981 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
    17041 tacaacaagg caaggcttga ccgacaatt
//
