LOCUS       sequence-243976-        9640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Nanoluc, copGFP .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9640)
  TITLE     Oka Lab plasmids
REFERENCE   2  (bases 1 to 9640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCDH-EF1-N-cG-Hph
FEATURES             Location/Qualifiers
     source          1..9640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    94..210
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        352..1530
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          583..1521
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1478..1498
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1543..1552
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1549..2061
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             2077..2133
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2158..2820
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             2905..2961
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2968..3993
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    4006..4594
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4059..4079)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4477..4488)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4668..4901
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4973..5094
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5010..5029)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5064..5083
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5134..5269
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5302..5318)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5302..5318)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5315..5337)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5326..5342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5350..5356,5357..5374,5375..5380))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5395..5416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5533..5550)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5704..6292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6463..7254,7255..7323))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7086..7105
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7324..7428)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7496..7514
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7552..7574)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7674..7693
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7887..7909
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7901..7918
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7902..7918
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7933..8159
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8160..8340
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8387..8512
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9005..9238
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9423..9467
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
       61 attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
      121 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
      181 acaaaaacaa attacaaaat tcaaaatttt atcgatatcg atagctatcg atatcgatat
      241 cgatagctat cgatatcgat atcgatagct ttgcaaagat ggataaagtt ttaaacagag
      301 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
      361 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
      421 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
      481 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
      541 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
      601 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
      661 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
      721 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
      781 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
      841 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
      901 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
      961 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     1021 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     1081 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     1141 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     1201 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     1261 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     1321 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     1381 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     1441 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     1501 agtttttttc ttccatttca ggtgtcgtga ggaattggat ccgccaccat ggtcttcaca
     1561 ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga ccaagtcctt
     1621 gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac tccgatccaa
     1681 aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat catcccgtat
     1741 gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct
     1801 gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat cgacggggtt
     1861 acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc
     1921 aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg
     1981 atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg
     2041 ctgtgcgaac gcattctggc gaagcttgga agcggagcta ctaacttcag cctgctgaag
     2101 caggctggag acgtggagga gaaccctgga cctatggaga gcgacgagag cggcctgccc
     2161 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg
     2221 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa
     2281 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac
     2341 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc
     2401 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc
     2461 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc
     2521 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg
     2581 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac
     2641 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc
     2701 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc
     2761 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat tgccttcgcc
     2821 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc
     2881 ggatctcgcg tcgacggaag cggagctact aacttcagcc tgctgaagca ggctggagac
     2941 gtggaggaga accctggacc tagatctatg aagaaacctg aactgacagc aacttctgtt
     3001 gagaagtttc tcattgaaaa atttgattct gtttctgatc tcatgcagct gtctgaaggt
     3061 gaagaaagca gagccttttc ttttgatgtt ggaggaagag gttatgttct gagggtcaat
     3121 tcttgtgctg atggttttta caaagacaga tatgtttaca gacactttgc ctctgctgct
     3181 ctgccaattc cagaagttct ggacattgga gaattttctg aatctctcac ctactgcatc
     3241 agcagaagag cacaaggagt cactctccag gatctccctg aaactgagct gccagctgtt
     3301 ctgcaacctg ttgctgaagc aatggatgcc attgcagcag ctgatctgag ccaaacctct
     3361 ggatttggtc cttttggtcc ccaaggcatt ggtcagtaca ccacttggag ggatttcatt
     3421 tgtgccattg ctgatcctca tgtctatcac tggcagactg tgatggatga cacagtttct
     3481 gcttctgttg ctcaggcact ggatgaactc atgctgtggg cagaagattg tcctgaagtc
     3541 agacacctgg tccatgctga ttttggaagc aacaatgttc tgacagacaa tggcagaatc
     3601 actgcagtca ttgactggtc tgaagccatg tttggagatt ctcaatatga ggttgccaac
     3661 attttttttt ggagaccttg gctggcttgc atggaacaac aaacaagata ttttgaaaga
     3721 agacacccag aactggctgg ttcccccaga ctgagagcct acatgctcag aattggcctg
     3781 gaccaactgt atcaatctct ggttgatgga aactttgatg atgctgcttg ggcacaagga
     3841 agatgtgatg ccattgtgag gtctggtgct ggaactgttg gaagaactca aattgcaaga
     3901 aggtctgctg ctgtttggac tgatggatgt gttgaagttc tggctgactc tggaaacagg
     3961 agaccctcca caagacccag agccaaggaa tgagaattcg tcgacaatca acctctggat
     4021 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     4081 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     4141 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     4201 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     4261 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     4321 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     4381 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     4441 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     4501 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     4561 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     4621 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     4681 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     4741 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4801 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4861 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     4921 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     4981 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5041 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     5101 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5161 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5221 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     5281 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5341 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5401 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5461 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5521 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5581 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5641 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5701 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5761 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5821 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5881 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5941 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6001 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6061 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6121 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6181 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6241 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6301 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6361 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6421 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6481 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6541 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6601 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6661 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6721 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6781 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6841 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6901 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6961 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7021 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7081 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7141 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7201 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7261 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7321 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7381 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7441 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     7501 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     7561 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     7621 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     7681 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     7741 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     7801 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     7861 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
     7921 caagctgacg cgtgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     7981 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     8041 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     8101 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     8161 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8221 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8281 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8341 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     8401 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     8461 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     8521 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     8581 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     8641 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     8701 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     8761 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     8821 aaacaaaagt aagaccaccg cacagcaagc ggccactgat cttcagacct ggaggaggag
     8881 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     8941 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     9001 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     9061 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     9121 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     9181 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     9241 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     9301 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     9361 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     9421 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     9481 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     9541 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     9601 cattatcgtt tcagacccac ctcccaaccc cgaggggacc
//
