LOCUS       sequence-243981-       10119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Luc2, copGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10119)
  TITLE     Oka Lab plasmids
REFERENCE   2  (bases 1 to 10119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCDH-EF1-Luc2-cG-BSD
FEATURES             Location/Qualifiers
     source          1..10119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    75..191
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        311..1489
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          542..1480
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1437..1457
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1502..1511
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1508..3157
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3173..3229
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3254..3916
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             4001..4054
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4055..4453
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    4466..5054
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4519..4539)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4937..4948)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5128..5361
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5433..5554
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5470..5489)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5524..5543
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5594..5729
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5762..5778)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5762..5778)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5775..5797)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5786..5802
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5810..5816,5817..5834,5835..5840))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5855..5876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5993..6010)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6164..6752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6923..7714,7715..7783))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7546..7565
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7784..7888)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7956..7974
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8012..8034)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8134..8153
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8347..8369
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8361..8378
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8362..8378
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8393..8619
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8620..8800
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8847..8972
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9465..9698
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9883..9927
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
       61 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
      121 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
      181 ttcaaaattt tatcgatatc gatagctatc gatatcgata tcgatagctt tgcaaagatg
      241 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
      301 agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
      361 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
      421 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
      481 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
      541 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
      601 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
      661 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
      721 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
      781 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
      841 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
      901 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
      961 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     1021 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     1081 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     1141 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     1201 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     1261 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     1321 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     1381 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     1441 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattggatc
     1501 cgccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga
     1561 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg
     1621 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga
     1681 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat
     1741 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat
     1801 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat
     1861 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa
     1921 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta
     1981 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga
     2041 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag
     2101 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg
     2161 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct
     2221 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg
     2281 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca
     2341 agactataag attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag
     2401 cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc
     2461 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca
     2521 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa
     2581 gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac
     2641 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat
     2701 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct
     2761 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct
     2821 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct
     2881 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg
     2941 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat
     3001 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt
     3061 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat
     3121 tctcattaag gccaagaagg gcggcaagat cgccgtgaag cttggaagcg gagctactaa
     3181 cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cctggaccta tggagagcga
     3241 cgagagcggc ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt
     3301 ggagttcgag ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa
     3361 gatgaagagc accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg
     3421 ctacggcttc taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc
     3481 catcaacaac ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct
     3541 gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt
     3601 gggcaccggc ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc
     3661 caccgtggag cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac
     3721 cttcagcctg cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa
     3781 gagcgccatc caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt
     3841 ggaggagctg cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac
     3901 ccccattgcc ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc
     3961 cggacccggc tccaccggat ctcgcgtcga cggcagtgga gagggcagag gaagtctgct
     4021 aacatgcggt gacgtcgagg agaatcctgg cccaatggcc aagcctttgt ctcaagaaga
     4081 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta
     4141 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata
     4201 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc
     4261 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc
     4321 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa
     4381 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt
     4441 gtgggagggc taagaattcg tcgacaatca acctctggat tacaaaattt gtgaaagatt
     4501 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4561 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4621 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4681 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4741 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4801 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4861 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4921 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4981 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5041 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta
     5101 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagataag
     5161 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5221 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5281 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5341 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     5401 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     5461 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5521 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     5581 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5641 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     5701 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca
     5761 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     5821 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     5881 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     5941 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6001 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6061 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6601 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6901 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6961 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7021 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7081 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7141 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7201 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7261 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7321 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7381 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7441 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7501 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7561 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7621 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7681 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7741 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7801 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7861 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     7921 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     7981 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8041 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8101 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     8161 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     8221 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     8281 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     8341 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctgacg cgtgtagtct
     8401 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     8461 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     8521 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     8581 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     8641 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8701 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8761 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     8821 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     8881 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     8941 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     9001 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     9061 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     9121 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     9181 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     9241 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     9301 cacagcaagc ggccactgat cttcagacct ggaggaggag atatgaggga caattggaga
     9361 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     9421 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     9481 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     9541 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     9601 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     9661 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     9721 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     9781 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     9841 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     9901 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     9961 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
    10021 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
    10081 ctcccaaccc cgaggggacc cgacaggccc gaaggaata
//
