LOCUS       sequence-244038-        9443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nesprin-2G force sensor with cerulean-F25-venus TSmodule.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Mayer CR, Arsenovic PT, Bathula K, Denis KB, Conway DE
  TITLE     Characterization of 3D Printed Stretching Devices for Imaging Force 
            Transmission in Live-Cells.
  JOURNAL   Cell Mol Bioeng. 2019 Jul 11;12(4):289-300. doi: 
            10.1007/s12195-019-00579-y. eCollection 2019 Aug.
  PUBMED    31719915
REFERENCE   2  (bases 1 to 9443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00579-y"; journalName: "Cell Mol Bioeng"; date: 
            "2019-07-11- 11"; volume: "12"; issue: "4"; pages: "289-300"
COMMENT     Sequence Label: nesprin TSmod 25
FEATURES             Location/Qualifiers
     source          1..9443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      309..318
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1776..1778,1779..1781,1782..2492)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2568..2570,2571..2573,2574..3284)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4420..4437)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4426..4650
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4696..5124
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4783..4802)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4993..5014
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5133..5153)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5138..5467
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5318..5453
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5380..5399
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5534..6328
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5588..5607)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6198..6217
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6502..6623
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6539..6558)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6593..6612
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6672..6688)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6672..6688)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6685..6707)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6696..6712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6720..6726,6727..6744,6745..6750))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6765..6786
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6903..6920)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7074..7662)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7154..7173)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7833..8624,8625..8693))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8456..8475
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8694..8798)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8873..8892)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9064..9443
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt aaacttaagc
      301 tttctagagc caccatggcc gctagccctg tgctgcccac ggaagatgga gagggtttct
      361 tgggcattga tgatctacac ttttcactgc aagctgagca ggaggacact cagaagaaaa
      421 ccttcacctg ctggataaac tcacagctgg caaagcacac acctccctca gttgtatctg
      481 acttattcgc agacatcaag aaggggcatg tcctcctgga cctgttggaa gtcctctcgg
      541 gacagcagtt gcctcgggat aaaggatcta atacattcca gtgcagaatc aacatagaac
      601 atgccctgac attcctgaaa aacagatcta tcaagctaat aaatatccat gttgctgaca
      661 ttgtagaagg aaacccgtcc attatccttg gcctgatctg gaccatcatc ctgcactttc
      721 atattgagaa gctcgctcag actctgtctt gcgattacaa tcaaccttca ccggaggttg
      781 tgagcgtggc tgcctcatct cctacctcaa gtcctcccac taagaagtgc tccaaggctc
      841 aagctcaggc aagatggcag tggtctgcca agaaggctct tctgcagtgg gctcaggagc
      901 agtgtgccag gtctgagtct gtcaatgtga cagattttaa gtcaagctgg agaaatggga
      961 tggctttttt ggctgtcatt catgccttgc gaccagacct gattgacatg gacagcatga
     1021 ggcacagatc caacaaagac aatctgaaag aggccttcag gatcgcagaa catgagctga
     1081 agatccccaa attgctggaa ccagaagatg tagatgtcgt gaatcctgat gagaagtcca
     1141 tcatgactta cgtggcgcag ttcctgaagt attcgaagga tgcgcctggc cccggggatt
     1201 ccacacaggc caaggtaagg gatgctctgg tctggctaac tctgcaagag aagagattcc
     1261 agaagatgct gaaggactca gcaagtgaga cctactgtaa caagtatcat agtctgctgt
     1321 catttatgga gtcactgaat gaagaaaagg aatccttcat tgatgtcctg tcactgaaag
     1381 ggaggatggg agagctgaat gaagatgaat cacggctgag acaaggctgg accagcctca
     1441 tgcaccaggt cgctgcgtgg agagcccaac tggatgacgc cttgccttca ccgctcaaag
     1501 agaccgaagc gtggctgaag gacatagagg gtgttgtgca agaaggggtg ccgacctccc
     1561 agagttactc tgaagccagg actctgatcc aagggaaact atcttcattc aagagtctga
     1621 tgggcagctt tgactatcat tcggatgttc tcatggcctt tcaatccaat gccgagaaga
     1681 gcctgccagc tgtgcctcct gttaaactgg aggagatgac aagacgaatc aacaacgttt
     1741 tgggtaaaaa cttcatccca ctcctggagc tcgagatggt gagcaagggc gaggagctgt
     1801 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1861 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1921 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg
     1981 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     2041 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     2101 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     2161 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac gccatcagcg
     2221 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
     2281 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     2341 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
     2401 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     2461 ggatcactct cggcatggac gagctgtaca aggggccagg tggtgcaggg cccggtggtg
     2521 caggtccagg tggtgcaggt ccaggtggtg caggtccagg tggtgctatg gtgagcaagg
     2581 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2641 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2701 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     2761 tgggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     2821 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2881 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2941 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3001 actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
     3061 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc
     3121 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
     3181 agtccaaact gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3241 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caaggcggcc gcgcaggagc
     3301 ttcacagcaa gctcaggctc aaacagaccg tgcagcagct caagtctgac atcggcagca
     3361 tcgctgcttg gcttgggaaa actgaggccg agctggaagc tctgaagttg gccgagcctc
     3421 cctctgacat ccaggaaata gcgctcaggg tgaagagact gcaagagata ctaaaagcct
     3481 tcgataccta caaggccttg atggtctccg tcaatgtgag ccacaaggaa tatctgccaa
     3541 gccagagccc cgaagccaca gaactccaaa atagactcca tcagctgagc ctgtcctggg
     3601 actcggtcca gggcgtattg gacagctggc gaggagactt acggcagtca ctcatgcagt
     3661 gccaggactt tcaccagctg agtcaagact tgctcctgtg gctagcgaca gcagagagcc
     3721 gcaggcagaa ggcgcatgtc accagcccag aggccgaccg ccaggtcctc ctggagtgtc
     3781 agaaagacct aatgcgccta gaaaaggagt tggtagcccg tcagcctcaa gtcagctcct
     3841 tgcgggagat ttcctccagc cttctggtga aggggcaagg agaagactac attgaggccg
     3901 aagagaaggt ccacgtgatc gagaagaaac tcaagcaact gcaagagcaa gtggctcaag
     3961 atctgatgtc cttgcagaga agcctggatc cggatgcttc tctaaccagc tttgatgagg
     4021 tggactcagg agaacagctc ccagcagcct ttgcaaagag tccaaggccc cgctggacct
     4081 tcttggaaga ggaagaggag gaggaggaga cagacagcag gatgccccac ctcgacagcc
     4141 ccggcagctc ccagccgaga cgctccttcc tctcaagggt gatcagggca gcgctaccgt
     4201 tgcagctgct tctgctgctg ctgctgctcc tggcctgcct gctacctgcc tctgaagatg
     4261 actacagctg cacccaggcc aacaactttg cccgatcctt ctaccccatg ctgcggtaca
     4321 ccaacgggcc acctcccacc tagaccggtg aattctgcag atatccagca cagtggcggc
     4381 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     4441 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4501 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4561 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4621 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     4681 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4741 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4801 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4861 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4921 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4981 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5041 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5101 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     5161 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5221 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5281 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5341 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5401 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5461 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     5521 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5581 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     5641 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     5701 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     5761 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     5821 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     5881 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     5941 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     6001 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     6061 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     6121 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     6181 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     6241 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     6301 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     6361 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6421 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6481 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     6541 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6601 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     6661 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6721 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6781 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6841 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6901 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6961 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7021 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7081 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7141 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7201 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7261 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7321 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7381 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7441 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7501 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7561 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7621 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7681 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7741 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7801 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7861 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7921 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7981 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8041 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8101 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8161 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8221 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8281 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8341 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8401 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8461 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8521 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8581 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8641 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8701 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8761 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8821 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     8881 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     8941 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     9001 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     9061 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     9121 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     9181 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     9241 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     9301 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     9361 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     9421 tattagtcat cgctattacc atg
//
