LOCUS       sequence-244039-        8882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Cre enzyme in mammalian cells with floxed genes to achieve 
            somatic knockout. GFP is simultaneously expressed for convenient 
            tracking the transfected cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Geng A, Qiu R, Murai K, Liu J, Wu X, Zhang H, Farhoodi H, Duong N, 
            Jiang M, Yee JK, Tsark W, Lu Q
  TITLE     KIF20A/MKLP2 regulates the division modes of neural progenitor cells
            during cortical development.
  JOURNAL   Nat Commun. 2018 Jul 13;9(1):2707. doi: 10.1038/s41467-018-05152-1.
  PUBMED    30006548
REFERENCE   2  (bases 1 to 8882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-13"; volume: "9(1):2707. doi"; pages: " 
            10.1038/s41467-018-05152-1"
COMMENT     Sequence Label: Hiv7CMV-Cremyc-2A-GFP
FEATURES             Location/Qualifiers
     source          1..8882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      1119..1254
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        1317..1696
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             1935..2115
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2162..2287
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2946..3179
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3260..3377
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3524..3827
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3828..4031
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4109..4128
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4211..5239
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             5240..5269
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5285..5341
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5342..5344,5345..5347,5348..6061)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5342..5344,5345..5347,5348..6058)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5648..5667)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5995..6016
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6108..6696
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6161..6181)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6579..6590)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6787..7020
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7041..7057)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7082..7101)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7083..7101)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7108..7125)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7108..7124)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7117..7139)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(7265..7720)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7402..7423)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7614..7633
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7747..7851
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7852..7920,7921..8712)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8070..8089)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggcctcgagg tcgagatccg
     1081 gtcgaccagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1141 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1201 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1261 tttgcaaaaa gcttcgacgg tatcgattgg ctcatgtcca acattaccgc catgttgaca
     1321 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     1381 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     1441 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     1501 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     1561 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     1621 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     1681 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     1741 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     1801 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     1861 cggtaggcgt gtacggaatt cggagtggcg agccctcaga tcctgcatat aagcagctgc
     1921 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     1981 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     2041 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     2101 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     2161 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     2221 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     2281 gagcgtcagt attaagcggg ggagaattag atcgatggga aaaaattcgg ttaaggccag
     2341 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     2401 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     2461 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     2521 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     2581 tagaggaaga gcaaaacaaa agtaagaaaa aagcacagca agcagcagct gacacaggac
     2641 acagcaatca ggtcagccaa aattacccta tagtgcagaa catccagggg caaatggtac
     2701 atcaggccat atcacctaga actttaaatg catgggtaaa agtagtagaa gagaaggctt
     2761 tcagcccaga agtgataccc atgttttcag cattatcaga aggagccacc ccacaagatt
     2821 taaacaccat gctaaacaca gtggggggac atcaagcagc catgcaaatg ttaaaagaga
     2881 ccatcaatga ggaagctgca ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     2941 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     3001 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     3061 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     3121 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     3181 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gatctacaaa
     3241 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     3301 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     3361 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggggatccc
     3421 ccgggctgca ggaattcgat gatccagtat taccgccatg cattagttat taatagtaat
     3481 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     3541 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3601 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     3661 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3721 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3781 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3841 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3901 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3961 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     4021 taagcagagc tggtttagtg aaccgtcaga tccgctagag cctctgctaa ccatgttcat
     4081 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     4141 tttggcaaag aatttcgagg ggcagagccg atcctgtaca ctttacttaa aaccattatc
     4201 tgagtgtgaa atgtccaatt tactgaccgt acaccaaaat ttgcctgcat taccggtcga
     4261 tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc gccaggcgtt
     4321 ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa
     4381 gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt atcttctata
     4441 tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc agctaaacat
     4501 gcttcatcgt cggtccgggc tgccacgacc aagtgacagc aatgctgttt cactggttat
     4561 gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt
     4621 cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgatc gctgccagga
     4681 tatacgtaat ctggcatttc tggggattgc ttataacacc ctgttacgta tagccgaaat
     4741 tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt taatccatat
     4801 tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta gcctgggggt
     4861 aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc cgaataacta
     4921 cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca gccagctatc
     4981 aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg gcgctaagga
     5041 tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg
     5101 agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg gctggaccaa
     5161 tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg caatggtgcg
     5221 cctgctggaa gatggcgatg aacaaaaact catctcagaa gaggatctgg aattcggatc
     5281 cggagccacg aacttctctc tgttaaagca agcaggagac gtggaagaaa accccggtcc
     5341 tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     5401 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     5461 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     5521 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     5581 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     5641 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     5701 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     5761 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     5821 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     5881 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     5941 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     6001 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtg
     6061 aggatcatca agctgggctg caggaattcg atatcaagct tatcgataat caacctctgg
     6121 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6181 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6241 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6301 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6361 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6421 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6481 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     6541 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6601 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6661 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga ctagccgtac
     6721 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6781 ggggactgga agggctaatt cactcccaaa gaagacaaga tctgcttttt gcctgtactg
     6841 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6901 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6961 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7021 gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccaattc
     7081 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
     7141 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     7201 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     7261 atggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     7321 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     7381 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     7441 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     7501 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     7561 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     7621 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     7681 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     7741 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7801 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7861 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7921 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7981 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8041 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     8101 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8161 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8221 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8281 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8341 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8401 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8461 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8521 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8581 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8641 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8701 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8761 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8821 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8881 tc
//
