LOCUS       sequence-244072-        6957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SB-transposon plasmid for stable expression of cpVenus[E172] cDNA as
            a FRET single color control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Havranek O, Xu J, Kohrer S, Wang Z, Becker L, Comer JM, Henderson J,
            Ma W, Man Chun Ma J, Westin JR, Ghosh D, Shinners N, Sun L, Yi AF, 
            Karri AR, Burger JA, Zal T, Davis RE
  TITLE     Tonic B-cell receptor signaling in diffuse large B-cell lymphoma.
  JOURNAL   Blood. 2017 Aug 24;130(8):995-1006. doi: 
            10.1182/blood-2016-10-747303. Epub 2017 Jun 23.
  PUBMED    28646116
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2016-10-747303"; journalName: "Blood"; date: 
            "2017-08-24- 24"; volume: "130"; issue: "8"; pages: "995-1006"
COMMENT     Sequence Label: pSBbi-Pur-cpVenus[E172]
FEATURES             Location/Qualifiers
     source          1..6957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1208..1217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1229..1246
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1769..2503
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     1903..1924
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2030..2051)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2291..2310)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(2557..2574)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2563..2787
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        3219..3323
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3324..3392,3393..4184)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3542..3561)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4355..4943
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4844..4863
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5097..5114
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     5488..5507
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    5506..5561
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     5607..5626
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(5726..6325)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5809..5829)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6306..6325
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        join(6957,1..1182)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
       61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccatactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt aaggtgtcgt gaaaactacc ccaagctggc
     1201 ctctgaggcc accatggtgc ggggttctca tcatcatcat catcatgacg aaaacattgt
     1261 gtgcagggtc atttgtacca cgggtcaaat tcccatccga gatttgtcag ctgatatttc
     1321 acaagtgctt aaggaaaaac gatccataaa gaaagtttgg acatttggta gaaacccagc
     1381 ctgtgactat catttaggaa acatttcaag actgtcaaat aagcatttcc aaatactact
     1441 aggagaagac ggtaaccttt tattgaatga catttccact aatgggacct ggttaaatgg
     1501 gcaaaaagtc gagaagaaca gcaatcagtt actgtctcaa ggtgatgaaa taaccgttgg
     1561 tgtaggcgtg gaatcagata ttttatctct ggtcattttc ataaacgaca aatttaagca
     1621 gtgcctcgag cagaacaaag ttgatcgctc tgcaggtaag ccaggcagcg gcgagggcag
     1681 caccaagggc ctggtcgacc ctcgtccgcg ctcgtgcacc tggccggacc ccaggccgga
     1741 gtttggaggt accggcggca gcgagctcat gggcggcgtg cagctcgccg accactacca
     1801 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
     1861 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     1921 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggag gtaccggtgg
     1981 atctatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2041 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2101 ctacggcaag ctgaccctga agctgatctg caccaccggc aagctgcccg tgccctggcc
     2161 caccctcgtg accaccctgg gctacggcct tcagtgcttc gcccgctacc ccgaccacat
     2221 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2281 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2341 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2401 gcacaagctg gagtacaact acaacagcca caacgtctat atcaccgccg acaagcagaa
     2461 gaacggcatc aaggccaact tcaagatccg ccacaacatc gagtaaggcc tgtcaggcca
     2521 agcttgggga tcaattctct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     2581 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     2641 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     2701 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     2761 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tgggcctact
     2821 agatagcgga ccccttaccg aaacatcgcc gcattctgca gaggagtcga acttagtgta
     2881 tgtaaacttc tgacccactg gaattgtgat acagtgaatt ataagtgaaa taatctgtct
     2941 gtaaacaatt gttggaaaaa tgacttgtgt catgcacaaa gtagatgtcc taactgactt
     3001 gccaaaacta ttgtttgtta acaagaaatt tgtggagtag ttgaaaaacg agttttaatg
     3061 actccaactt aagtgtatgt aaacttccga cttcaactgt atagggatcc aattttaaca
     3121 aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3181 ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3241 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     3301 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     3361 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     3421 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     3481 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     3541 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     3601 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     3661 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     3721 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     3781 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     3841 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     3901 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     3961 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4021 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4081 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4141 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     4201 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     4261 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     4321 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4381 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4441 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4501 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4561 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4621 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4681 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4741 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4801 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4861 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4921 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4981 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5041 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5101 agtcagtgag cgaggaagcg gatccctata cagttgaagt cggaagttta catacacctt
     5161 agccaaatac atttaaactc actttttcac aattcctgac atttaatcct agtaaaaatt
     5221 ccctgtctta ggtcagttag gatcaccact ttattttaag aatgtgaaat atcagaataa
     5281 tagtagagag aatgattcat ttcagctttt atttctttca tcacattccc agtgggtcag
     5341 aagtttacat acactcgact cctctgcaga atgcggcgat gtttcggtaa ggggtcccga
     5401 gtagctagtt catggcagcc agcatatggc atatgttgcc aaactctaaa ccaaatactc
     5461 attctgatgt tttaaatgat ttgccctccc atatgtcctt ccgagtgaga gacacaaaaa
     5521 attccaacac actattgcaa tgaaaataaa tttcctttat tagccagaag tcagatgctc
     5581 aaggggcttc atgatgtccc cataattttt ggcagaggga aaaagatctc agtggtattt
     5641 gtgagccagg gcattggcca caccagccac caccttctga taggcagcct gcacctgagg
     5701 agtgaattca cgacaggcct tcgaatcagg caccgggctt gcgggtcatg caccaggtgc
     5761 gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg
     5821 ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct
     5881 ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc
     5941 cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca
     6001 tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg
     6061 cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt
     6121 cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca
     6181 gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt
     6241 cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc
     6301 gcaccgtggg cttgtactcg gtcatggtgg cctcaggtgc agaggtttct acagggaaag
     6361 agagaaaggc tacagttagt ttgctgtgat gatgctaagt gccgaggtca gatgtctatg
     6421 gattgtacac ggaatcccaa cgcaggaacc aaaaaacact cacccaaaaa aatggcgcta
     6481 aggccgagag aaaggcgaag ttccgtctac ggctatatac cttgaaacag gggcggcaga
     6541 agtgacgcaa cgcggttgcg cggacggctt cctgtgtttt cattggtcga agcgttctgc
     6601 gcgtgcgcag agattcccca gggaaggcgg gccctcttcc gaatgtccaa tccggacatt
     6661 cttccggttg gacctccgcg gtcagcgctg tgcccggcga gagatcacgt ggggcgcgga
     6721 ggcggtgctg ctggggcacg gccgtccagc ctcggcggcc atatttttga ggggctgttc
     6781 atctcgttca cacgctctgt ccgccatgtt tgtgagtgga agcgccatta ccccttcaag
     6841 cgactgaagg ctgcagggcc tctggtggcc cgcatgggga gaccagaccc gccaggcccg
     6901 cctttccgca ctcagtccgg gcttacttta ttttgtgaga cagggtctcg cctagag
//
