LOCUS       sequence-244150-        5743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes H. sapiens codon optimized Integrase 9..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Gomide MS, Sales TT, Barros LRC, Limia CG, de Oliveira MA, 
            Florentino LH, Barros LMG, Robledo ML, Jose GPC, Almeida MSM, Lima 
            RN, Rehen SK, Lacorte C, Melo EO, Murad AM, Bonamino MH, Coelho CM, 
            Rech E
  TITLE     Genetic switches designed for eukaryotic cells and controlled by 
            serine integrases.
  JOURNAL   Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.
  PUBMED    32444777
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-22"; volume: "3(1):255. doi"; pages: " 
            10.1038/s42003-020-0971-8"
COMMENT     Sequence Label: INCTbiosyn-pUB-HspINT9
FEATURES             Location/Qualifiers
     source          1..5743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        46..1257
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1094..1113
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     complement(2767..2786)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2832..2887
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2886..2905)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3246..3262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3246..3262)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3259..3281)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3270..3286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3294..3300,3301..3318,3319..3324))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3339..3360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3419..3614
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3465..3600
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3620..3754
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3657..3676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3711..3730
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3822..3839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3993..4581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4073..4092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4752..5543,5544..5612))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5375..5394
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5613..5717)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agtaacccgt gtcggctcca gatctggcct ccgcgccggg
       61 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag
      121 ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat
      181 aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg
      241 actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc
      301 tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg
      361 cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg
      421 tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt
      481 ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct
      541 gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc
      601 tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag
      661 gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa
      721 gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc
      781 actgactgga gaactcgggt ttgtcgtctg gttgcggggg cggcagttat gcggtgccgt
      841 tgggcagtgc acccgtacct ttgggagcgc gcgcctcgtc gtgtcgtgac gtcacccgtt
      901 ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc
      961 cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac
     1021 ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat
     1081 cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt
     1141 tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag
     1201 tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgaa
     1261 gcttgggctg caggtcgagc tcaagcttcg aattcatgaa ggtggccatt tacacgcgag
     1321 tgagtacttt ggaacagaag gagaaggggc atagtattga ggaacaggaa cgcaagctgc
     1381 gggcctacag tgacatcaac gattggaaaa tccataaagt atacactgac gcaggataca
     1441 gcggggcgaa aaaggatcgc ccagcacttc aggaaatgtt gaatgagatt gataattttg
     1501 acctcgtcct cgtctacaag ttggataggc ttacaaggag tgtgaaagac ctccttgaga
     1561 ttctcgagct gtttgaaaac aagaacgtcc tgtttagatc agccactgag gtctacgata
     1621 ctacgtcagc tatgggtcgg ctctttgtta ctctggttgg cgctatggct gagtgggaac
     1681 gcacgacaat tcaggagaga actgcgatgg ggcgacgagc gagtgctcgg aaaggacttg
     1741 ccaagactgt tccaccgttt tactacgaca gggtgaatga taagtttgtt cccaacgaat
     1801 ataaaaaggt cctgcgcttc gcggtcgaag aggcaaaaaa agggacaagc ttgcgagaga
     1861 tcaccattaa actgaataac tccaagtata aagctccatt gggcaagaat tggcatcgaa
     1921 gtgtgatagg aaatgcactg acgagcccgg ttgccagggg gcacttggtc tttggcgaca
     1981 tttttgtcga gaacacccat gaggcgatta taagtgaaga agagtacgag gagataaaac
     2041 tcaggatctc tgagaaaacg aatagcacaa tagttaagca caacgccata ttcagatcaa
     2101 aactgctgtg tcccaattgc aaccagaagc tgacactgaa taccgtgaaa cataccccaa
     2161 agaacaagga ggtttggtat tctaaactgt acttctgcag taattgcaag aacacaaaga
     2221 acaaaaatgc atgcaacatt gacgagggag aggtgctgaa gcaattttat aattacctga
     2281 agcaatttga cctgacgtcc tacaaaatag aaaaccagcc gaaagaaatt gaggatgtgg
     2341 ggatcgacat cgaaaagctt cgcaaggagc gggcacgatg ccagacactt ttcattgagg
     2401 gcatgatgga caaagatgaa gcttttccca tcatttcaag aatagataag gagatccatg
     2461 agtatgagaa gcggaaggat aacgacaagg ggaagacgtt caactacgag aagataaaga
     2521 atttcaagta ctcccttctt aatgggtggg aactcatgga agacgagctg aaaacggaat
     2581 tcatcaaaat ggctatcaaa aacatccact tcgagtatgt gaaggggatt aaaggtaaaa
     2641 gacaaaatag tctcaagata accggtatag agttttacta agcggccgca ctcctcaggt
     2701 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     2761 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     2821 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     2881 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     2941 ggtttagagt ttggcaacat atgccatatg ctggctgcca tgaacaaagg tggctataaa
     3001 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
     3061 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     3121 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     3181 tcatagctgt ccctcttctc ttatgaagat ccctcgacct gcagcccaag cttggcgtaa
     3241 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     3301 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3361 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc
     3421 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3481 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3541 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3601 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa
     3661 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3721 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc
     3781 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     3841 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3901 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3961 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4021 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4081 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4141 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     4201 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4261 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4321 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4381 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     4441 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4501 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4561 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4621 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4681 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4741 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4801 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4861 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4921 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4981 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5041 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5101 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5161 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5221 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5281 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5341 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5401 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5461 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5521 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5581 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5641 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5701 caaatagggg ttccgcgcac atttccccga aaagtgccac ctg
//
