LOCUS       sequence-244151-        5782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes H. sapiens codon optimized Integrase 13..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Gomide MS, Sales TT, Barros LRC, Limia CG, de Oliveira MA, 
            Florentino LH, Barros LMG, Robledo ML, Jose GPC, Almeida MSM, Lima 
            RN, Rehen SK, Lacorte C, Melo EO, Murad AM, Bonamino MH, Coelho CM, 
            Rech E
  TITLE     Genetic switches designed for eukaryotic cells and controlled by 
            serine integrases.
  JOURNAL   Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.
  PUBMED    32444777
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-22"; volume: "3(1):255. doi"; pages: " 
            10.1038/s42003-020-0971-8"
COMMENT     Sequence Label: INCTbiosyn-pUB-HspINT13
FEATURES             Location/Qualifiers
     source          1..5782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        46..1257
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1094..1113
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     complement(2806..2825)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2871..2926
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2925..2944)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3285..3301)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3285..3301)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3298..3320)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3309..3325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3333..3339,3340..3357,3358..3363))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3378..3399
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3458..3653
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3504..3639
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3659..3793
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3696..3715)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3750..3769
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3861..3878)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4032..4620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4112..4131)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4791..5582,5583..5651))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5414..5433
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5652..5756)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agtaacccgt gtcggctcca gatctggcct ccgcgccggg
       61 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag
      121 ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat
      181 aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg
      241 actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc
      301 tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg
      361 cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg
      421 tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt
      481 ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct
      541 gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc
      601 tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag
      661 gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa
      721 gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc
      781 actgactgga gaactcgggt ttgtcgtctg gttgcggggg cggcagttat gcggtgccgt
      841 tgggcagtgc acccgtacct ttgggagcgc gcgcctcgtc gtgtcgtgac gtcacccgtt
      901 ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc
      961 cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac
     1021 ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat
     1081 cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt
     1141 tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag
     1201 tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgaa
     1261 gcttgggctg caggtcgagc tcaagcttcg aattcatggc agtcggaatt tatattcgag
     1321 ttagtaccca agaacaggcg tccgaaggcc actctataga atcccaaaaa aagaagctgg
     1381 ccagctactg cgagattcag ggatgggacg attatcgctt ctacatcgag gaaggtatct
     1441 ccgggaaaaa cacaaatcgg ccaaagttga aactgctcat ggaacatatt gaaaagggaa
     1501 agattaatat tctcctcgta tatcgcctcg accgactcac acgctctgtg atcgatttgc
     1561 acaagctgct taactttttg caggagcatg ggtgtgcatt taaatcagcg acagagacct
     1621 atgatacgac gacagcgaat ggtcgcatgt ccatgggtat tgtcagtctc ttggcccaat
     1681 gggaaacaga gaatatgtcc gaacggataa aacttaacct tgaacacaag gtgctcgtcg
     1741 aaggggagag agtcggagcg atcccctacg gattcgacct ctccgacgac gaaaagcttg
     1801 ttaagaacga aaaatctgcc atattgctgg acatggtgga aagagtcgag aacgggtggt
     1861 cagtcaatcg gattgtcaac tatttgaatc ttactaataa tgatcggaac tggtccccga
     1921 acggtgttct gcgcctgctg cggaacccgg ccctgtacgg cgccacacgg tggaacgaca
     1981 agatcgctga aaacacgcac gaaggcatta tctctaaaga aagatttaat cggcttcagc
     2041 agatattggc agaccgctcc atacaccacc gaagggacgt gaaaggtact tatatatttc
     2101 aaggggtctt gcgatgtccc gtctgcgacc aaaccctgag tgtaaaccgc tttattaaaa
     2161 aacggaagga tgggacggag tattgcggcg tcctgtatag atgccaacca tgcataaaac
     2221 agaataaata caatcttgcc atcggagaag ctcgattcct gaaggctctc aatgaatata
     2281 tgtccacagt agagtttcag acggttgagg atgaagtaat acccaaaaaa agtgagcgag
     2341 aaatgctcga gtcacagctt caacagatcg ctagaaagcg agagaagtac caaaaggcgt
     2401 gggcctcaga ccttatgagt gacgatgagt ttgaaaagct gatggtagaa acgagagaaa
     2461 cgtatgatga gtgtaaacaa aagcttgagt cttgtgaaga cccaataaag atcgacgaga
     2521 cttaccttaa agagatagtt tatatgttcc accagacgtt caacgatctg gagtctgaga
     2581 agcaaaaaga atttatctca aagtttattc gcacgattcg atacactgta aaggagcaac
     2641 aaccaattcg gccggataaa tctaagacgg ggaaagggaa gcagaaagtt atcataacgg
     2701 aggttgagtt ctatcagtaa gcggccgcac tcctcaggtg caggctgcct atcagaaggt
     2761 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc
     2821 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     2881 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     2941 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
     3001 tgccatatgc tggctgccat gaacaaaggt ggctataaag aggtcatcag tatatgaaac
     3061 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     3121 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     3181 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     3241 tatgaagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
     3301 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3361 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3421 gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca
     3481 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3541 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     3601 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa
     3661 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3721 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3781 tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3841 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3901 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3961 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4021 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4081 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4141 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4201 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     4261 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4321 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4381 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4441 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     4501 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4561 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4621 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4681 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4741 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4801 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4861 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4921 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4981 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5041 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5101 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5161 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5221 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5281 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5341 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5401 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5461 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5521 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5581 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5641 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5701 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5761 tttccccgaa aagtgccacc tg
//
