LOCUS       sequence-244153-        6147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes H. sapiens codon optimized  Integrase Bxb1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Gomide MS, Sales TT, Barros LRC, Limia CG, de Oliveira MA, 
            Florentino LH, Barros LMG, Robledo ML, Jose GPC, Almeida MSM, Lima 
            RN, Rehen SK, Lacorte C, Melo EO, Murad AM, Bonamino MH, Coelho CM, 
            Rech E
  TITLE     Genetic switches designed for eukaryotic cells and controlled by 
            serine integrases.
  JOURNAL   Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.
  PUBMED    32444777
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-22"; volume: "3(1):255. doi"; pages: " 
            10.1038/s42003-020-0971-8"
COMMENT     Sequence Label: INCTbiosyn-pUB-HspINTBxb1
FEATURES             Location/Qualifiers
     source          1..6147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     996..1015
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1014..1069
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1115..1134
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     promoter        complement(2729..3940)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(2873..2892)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        complement(3959..3977)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3998..4014)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3998..4014)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4011..4033)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4022..4038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4046..4052,4053..4070,4071..4076))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4091..4112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4229..4246)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4400..4988)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4480..4499)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5159..5950,5951..6019))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5782..5801
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6020..6124)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacccatga
      661 ttacgccaag cttgggctgc aggtcgaggg atcttcataa gagaagaggg acagctatga
      721 ctgggagtag tcaggagagg aggaaaaatc tggctagtaa aacatgtaag gaaaatttta
      781 gggatgttaa agaaaaaaat aacacaaaac aaaatataaa aaaaatctaa cctcaagtca
      841 aggcttttct atggaataag gaatggacag cagggggctg tttcatatac tgatgacctc
      901 tttatagcca cctttgttca tggcagccag catatggcat atgttgccaa actctaaacc
      961 aaatactcat tctgatgttt taaatgattt gccctcccat atgtccttcc gagtgagaga
     1021 cacaaaaaat tccaacacac tattgcaatg aaaataaatt tcctttatta gccagaagtc
     1081 agatgctcaa ggggcttcat gatgtcccca taatttttgg cagagggaaa aagatctcag
     1141 tggtatttgt gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc
     1201 acctgaggag tgcggccgcc taagacatcc ctgtatgcag tctctccact acgctaccaa
     1261 gcctcaagtg ttgttcatac tcctgcaaat ctccgaaatc gatagtcctg gtaagccccc
     1321 ctctaacgtc gaacgtcagc cggacgttca tgctcctaag ccacgtattc ttcgccgcag
     1381 tatcttgctc gcgccaccaa tccccgaacc gctggcctgt ttccctccat tcccatccag
     1441 aaggtctagc ttccaacccc tccaattcct cttgtctcgc ggcgagtgcc gctattctgg
     1501 catcaagtgc ctcgcgttgc ggggagccag ctcggtacgc tggagagcca ataagggatg
     1561 tgagatcgac cagttctgca ttgacttcag caagttctac agcggagtcg gacccagcta
     1621 cccatacctt ttccaggcgt tctgcgtcgc caagcaagtc aaggacttgt tcttcgcaga
     1681 atgcatccca ctcagccatc gctactgtac cgtttccaca atgcttcggg aagcccatgc
     1741 tgcggcatct atagcgggga tgttttctcc cacccccggc aaacttgtat gctggctctc
     1801 cacaaacggc gcaaaacaat actcgaagca acaaacttgg tgtgctcacc gccggtttag
     1861 cccgtgatgt tttgaccagc tcagcccgca gtgcttccag ttgctcgcga gtaagaattg
     1921 gttcggcccg aaccaggggc gctccgtcat cgtccctaac ggttttgccg ttaagggttg
     1981 cgtatcccag catggcttcg ctaatcatgg atcttttcag agcagtagca ctccactccc
     2041 gaccctgtgg ttccctccct tgcagctggg cgaaataatc tttcgggctc aaaactcccc
     2101 ggcgattcaa atcatgagct accaggtgaa ggggctcgtg attgtccaca actcgatggt
     2161 acacttcaag gattctttcc cgttggaccg ggtcgggcac caaccgccat tcgccatcca
     2221 ctctagtcgg caggtagccc cagggtggga gactgccgcg gtacttgcct gccctgatgt
     2281 taaagtgcgc ggcgctccga tttcgctctt taatagcttc caattccatt tgtgcaacgg
     2341 tacccatcag agctatgaca actgctgcaa acggtgtagt tgtatcaaag tgtgcttccg
     2401 tagcggagac aaccaatttt ttgtgatctt ctgcccaatg aaccaattgt tgaagatggc
     2461 gaatacttct tgtcaacctg tcgactcgat aggcaacgat cacatcgaat ggttgctctt
     2521 cgaaagcgag ccatcgagcc aaattcgggc gccgcttcct atcgaaggga tctactgcac
     2581 cggaaacatc gaggtcttcc gccactccaa cgacgtccca gccccgttgc gcacagagct
     2641 gctggcagct ttcaagttgc ctctcagggc tggtcgtagc gtcagtaact ctggagagcc
     2701 taattaccac cagtgcccgc atgaattcgt ctaacaaaaa agccaaaaac ggccagaatt
     2761 tagcggacaa tttactagtc taacactgaa aattacatat tgacccaaat gattacattt
     2821 caaaaggtgc ctaaaaaact tcacaaaaca cactcgccaa ccccgagcgc atagttcaaa
     2881 accggagctt cagctactta agaagatagg tacataaaac cgaccaaaga aactgacgcc
     2941 tcacttatcc ctcccctcac cagaggtccg gcgcctgtcg attcaggaga gcctacccta
     3001 ggcccgaacc ctgcgtcctg cgacggagaa aagcctaccg cacacctacc ggcaggtggc
     3061 cccaccctgc attataagcc aacagaacgg gtgacgtcac gacacgacga ggcgcgcgct
     3121 cccaaaggta cgggtgcact gcccaacggc accgcataac tgccgccccc gcaaccagac
     3181 gacaaacccg agttctccag tcagtgacaa acttcacgtc agggtcccca gatggtgccc
     3241 cagcccatct cacccgaata agagctttcc cgcattagcg aaggcctcaa gaccttgggt
     3301 tcttgccgcc caccatgccc cccaccttgt ttcaacgacc tcacagcccg cctcacaagc
     3361 gtcttccatt caagactcgg gaacagccgc cattttgctg cgctcccccc aacccccagt
     3421 tcagggcaac cttgctcgcg gacccagact acagcccttg gcggtctctc cacacgcttc
     3481 cgtcccaccg agcggcccgg cggccacgaa agccccggcc agcccagcag cccgctactc
     3541 accaagtgac gatcacagcg atccacaaac aagaaccgcg acccaaatcc cggctgcgac
     3601 ggaactagct gtgccacacc cggcgcgtcc ttatataatc atcggcgttc accgccccac
     3661 ggagatccct ccgcagaatc gccgagaagg gactactttt cctcgcctgt tccgctctct
     3721 ggaaagaaaa ccagtgccct agagtcaccc aagtcccgtc ctaaaatgtc cttctgctga
     3781 tactggggtt ctaaggccga gtcttatgag cagcgggccg ctgtcctgag cgtccgggcg
     3841 gaaggatcag gacgctcgct gcgcccttcg tctgacgtgg cagcgctcgc cgtgaggagg
     3901 ggggcgcccg cgggaggcgc caaaacccgg cgcggaggcc gagctccagc ttttgttccc
     3961 tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     4021 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     4081 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     4141 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     4201 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4261 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4321 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4381 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4441 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4501 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4561 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4621 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4681 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4741 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4801 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     4861 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4921 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4981 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5041 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5101 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5161 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5221 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5281 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5341 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5401 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5461 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5521 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5581 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5641 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5701 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5761 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5821 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5881 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5941 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6001 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6061 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6121 cgcgcacatt tccccgaaaa gtgccac
//
