LOCUS       sequence-244189-        4060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Full MCS, lacks araC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Santos-Moreno J, Schaerli Y
  TITLE     A Framework for the Modular and Combinatorial Assembly of Synthetic 
            Gene Circuits.
  JOURNAL   ACS Synth Biol. 2019 Jul 19;8(7):1691-1697. doi: 
            10.1021/acssynbio.9b00174. Epub 2019 Jun 24.
  PUBMED    31185158
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00174"; journalName: "ACS Synth Biol"; date: 
            "2019-07-19- 19"; volume: "8"; issue: "7"; pages: "1691-1697"
COMMENT     Sequence Label: pC-0_v4
FEATURES             Location/Qualifiers
     source          1..4060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(975..993)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      989..1036
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1045..1131
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1223..1250
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             1753..1944
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1938..1960)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2046..2185
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2101..2120
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2200..2217)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2371..2959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2451..2470)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3130..3921,3922..3990))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3753..3772
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(3991..4060,1..35))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
       61 gaattcatag gatagattct ggaaacttta ccgtccgagc tcaggcttac cttactcgag
      121 caataaacag ttgatagggc ttctccgtta ccatggttca gccaaaaaac ttaagaccgc
      181 cggtcttgtc cactaccttg cagtaatgcg gtggacagga tcggcggttt tcttttctct
      241 tctcaattct tctgacctgt aacgaataat agatagtaaa gtagtctccg attgagtttt
      301 ctctgccgag tcccacccag ttctgtgatt tcagtaagtt ggtaattgat acactgttgc
      361 gagaactgct gcctggtagt agataggttg ttattgagta agaaggtaaa gtgaacgaaa
      421 tccctgaaac tgagactgta gaaaataagc ttgtccagac tattggatcc aagagatttc
      481 tacacgattg agcactgtct cctgcaggct cggtaccaaa ttccagaaaa gaggcctccc
      541 gaaagggggg ccttttttcg ttttggtcct aatagataaa ggataggtct ggtagtgttg
      601 ttcgttctcg caggtaaatc aataatactc agcagttccg tagacttttc agtgggacag
      661 ggtagcgata acagatagat tgtaataaga cacagtaggt gctcgtagtt gcgtgaagag
      721 aaccgctcag gaaatccagt cagaagtatt ggtaatcgtt gaaaactcag tcgacgcact
      781 tactgaagac gtcctattac actcgtcgtt ggaaactgaa gatgcggccg cggaaacaca
      841 gaaaaaagcc cgcacctgac agtgcgggct ttttttttcg accaaaggta gcgaacgacg
      901 agtcactgtt gaggataaat actttctcta ctaggcgcct gttacacagg tcctcagcgg
      961 cgcgcctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     1021 cttgaggggt tttttgccag gcatcaaata aaacgaaagg ctcagtcgga agactgggcc
     1081 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
     1141 gcggatttga acgttgtgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
     1201 actgccaggc atcaaactaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcag
     1261 atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg
     1321 acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag
     1381 cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc
     1441 ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg
     1501 ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta
     1561 acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc
     1621 agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc
     1681 gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac
     1741 acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga
     1801 agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac
     1861 atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc
     1921 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     1981 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     2041 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     2101 cggcatcaga gcagattgta ctgagagtgc accattgcgg tgtgaaatac cgcacagatg
     2161 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2221 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2281 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2341 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2401 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2461 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2521 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2581 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2641 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2701 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2761 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     2821 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2881 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2941 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3001 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3061 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3121 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3181 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3241 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3301 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3361 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3421 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
     3481 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3541 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3601 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3661 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3721 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     3781 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3841 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3901 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3961 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4021 ttatcagggt tattgtctca tgagcggata catatttgaa
//
