LOCUS       sequence-244200-       13365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCas9-VP64 driven by the bottleneck promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13365)
  AUTHORS   Buchman A, Shriner I, Yang T, Liu J, Antoshechkin I, Marshall JM, 
            Perry MW, Akbari OS
  TITLE     Engineered reproductively isolated species drive reversible 
            population replacement.
  JOURNAL   Nat Commun. 2021 Jun 2;12(1):3281. doi: 10.1038/s41467-021-23531-z.
  PUBMED    34078888
REFERENCE   2  (bases 1 to 13365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-2"; volume: "12(1):3281. doi"; pages: " 
            10.1038/s41467-021-23531-z"
COMMENT     Sequence Label: OA-986E
FEATURES             Location/Qualifiers
     source          1..13365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             731..778
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(779..802,803..805,806..3361,3362..3364,3365..4879)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4934..4954
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4979..5128
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5150..5203
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5204..5602
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             join(6843..6845,6846..6848,6849..7562)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7149..7168)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7496..7517
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7684..7805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7721..7740)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7775..7794
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8470..8504
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8685..8701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8685..8701)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8698..8720)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8709..8725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8733..8739,8740..8757,8758..8763))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8778..8799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8916..8933)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9087..9675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9167..9186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9846..10637,10638..10706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10469..10488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10707..10811)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10879..10897
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10935..10957)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11057..11076
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11270..11292
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11284..11301
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11285..11301
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   12005..12067
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    13101..13170
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 cggccgcatt agatgaaccc catgggatcc tgacacctca tttcgccttt tgcacagata
       61 ttttcccatt ccatctgtgt ttgattgcgt atttctcttg gaatatatgg aaattgcagt
      121 agtatgctta atgtataaca ccgtttttat ttcgtccctc atttccttct attattttgt
      181 cggctgtgaa ttgcagagtg tcgggtgaat cattaattgc attgattttg attttaacga
      241 gccaggtaat gaaacgtcaa cagtgtaaag tggccataaa cccctgcgga ttgcttgtag
      301 aaaccctgaa catctgtcgc gtttaatgtt tttaagctat ttatgaatcg ataccctcgc
      361 tgatcctttt tggtggtcct tataaaagtg tgcagattag ccgtcagtct tttgccaaaa
      421 attggtgcgc ggaaaacacg caaaactcgc gtgttcgggt tgcaattacc gggaaaacgc
      481 tcctttgaca aatcttcctt ttttactttc atagcttagg tagtggtctc aggtagtttc
      541 ccggaattaa gttaggctgg gtatcgccta tcgggaacgg ctacctgaac ttttggcacc
      601 agctgtcggg ggtgaaaact ggaccaggta gtctttagaa gtgcacctat ataaggtctg
      661 cgttcctggc ctcaacagca cattcgtact tcaaccgtca acgaattcgg ctgtttaaac
      721 tgccaccatg aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaagga
      781 caagaagtac agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac
      841 cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag
      901 catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac
      961 ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct
     1021 gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga
     1081 agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat
     1141 cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact
     1201 ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat
     1261 caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga
     1321 caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa
     1381 cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct
     1441 ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gcaacctgat
     1501 tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc
     1561 caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat
     1621 cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct
     1681 gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat
     1741 caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca
     1801 gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta
     1861 cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa
     1921 gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca
     1981 gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat
     2041 tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa
     2101 gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt
     2161 cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt
     2221 ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct
     2281 gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa
     2341 cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg
     2401 cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa
     2461 gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg
     2521 cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa
     2581 ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac
     2641 cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct
     2701 gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct
     2761 gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt
     2821 cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct
     2881 gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga
     2941 gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa
     3001 ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga
     3061 aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa
     3121 gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga
     3181 aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat
     3241 gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg accacatcgt
     3301 gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa
     3361 ggcccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta
     3421 ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa
     3481 ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt
     3541 ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa
     3601 gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct
     3661 ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca
     3721 ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc
     3781 taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat
     3841 cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat
     3901 catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct
     3961 gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac
     4021 cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac
     4081 aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag
     4141 aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc
     4201 tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga
     4261 gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct
     4321 ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc
     4381 cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa
     4441 gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta
     4501 tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca
     4561 caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct
     4621 ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat
     4681 cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc
     4741 cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt
     4801 gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct
     4861 gtctcagctg ggaggcgaca gcgctggagg aggtggaagc ggaggaggag gaagcggagg
     4921 aggaggtagc ggacctaaga aaaagaggaa ggtggcggcc gctggatccg gacgggctga
     4981 cgcattggac gattttgatc tggatatgct gggaagtgac gccctcgatg attttgacct
     5041 tgacatgctt ggttcggatg cccttgatga ctttgacctc gacatgctcg gcagtgacgc
     5101 ccttgatgat ttcgacctgg acatgctgat taactgtaca ggcagtggag agggcagagg
     5161 aagtctgcta acatgcggtg acgtcgagga gaatcctggc ccaatggcca agcctttgtc
     5221 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     5281 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     5341 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     5401 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     5461 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     5521 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     5581 tggttatgtg tgggagggct aattaattaa ctagaatgaa tcgtttttaa aataacaaat
     5641 caattgtttt ataatattcg tacgattctt tgattatgta ataaaatgtg atcattagga
     5701 agattacgaa aaatataaaa aatatgagtt ctgtgtgtat aacaaatgct gtaaacgcca
     5761 caattgtgtt tgttgcaaat aaacccatga ttatttgatt aaaattgttg ttttctttgt
     5821 tcatagacaa tagtgtgttt tgcctaaacg tgtactgcat aaactccatg cgagtgtata
     5881 gcgagctagt ggctaacgct tgccccacca aagtagattc gtcaaaatcc tcaatttcat
     5941 caccctcctc caagtttaac atttggccgt cggaattaac ttctaaagat gccacataat
     6001 ctaataaatg aaatagagat tcaaacgtgg cgtcatcgtc cgtttcgacc atttccgaaa
     6061 agaactcggg cataaactct atgatttctc tggacgtggt gttgtcgaaa ctctcaaagt
     6121 acgcagtcag gaacgtgcgc gacatgtcgt cgggaaactc gcgcggaaac atgttgttgt
     6181 aaccgaacgg gtcccatagc gccaaaacca aatctgccag cgtcaataga atgagcacga
     6241 tgccgacaat ggagctggct tggatagcga ttcgagttaa cggccggggc gcgcccatac
     6301 tcggtggcct ccccaccacc aacttttttg cactgcaaaa aaacacgctt ttgcacgcgg
     6361 gcccatacat agtacaaact ctacgtttcg tagactattt tacataaata gtctacaccg
     6421 ttgtatacgc tccaaataca ctaccacaca ttgaaccttt ttgcagtgca aaaaagtacg
     6481 tgtcggcagt cacgtaggcc ggccttatcg ggtcgcgtcc tgtcacgtac gaatcacatt
     6541 atcggaccgg acgagtgttg tcttatcgtg acaggacgcc agcttcctgt gttgctaacc
     6601 gcagccggac gcaactcctt atcggaacag gacgcgcctc catatcagcc gcgcgttatc
     6661 tcatgcgcgt gaccggacac gaggcgcccg tcccgcttat cgcgcctata aatacagccc
     6721 gcaacgatct ggtaaacaca gttgaacagc atctgttaca gcgacacaac atgagccggt
     6781 ccaacaacgc caacgcgccc acgccatcca accgccgccg caacctgtct ctggtgctcg
     6841 agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     6901 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     6961 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     7021 ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc gaccacatga
     7081 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     7141 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     7201 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     7261 acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac aagcagaaga
     7321 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     7381 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     7441 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     7501 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     7561 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     7621 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     7681 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7741 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7801 tcttaaagct tggccggccg atctcggatc tgacaatgtt cagtgcagag actcggctac
     7861 gcctcgtgga ctttgaagtt gaccaacaat gtttattctt acctctaata gtcctctgtg
     7921 gcaaggtcaa gattctgtta gaagccaatg aagaacctgg ttgttcaata acattttgtt
     7981 cgtctaatat ttcactaccg cttgacgttg gctgcacttc atgtacctca tctataaacg
     8041 cttcttctgt atcgctctgg acgtcatctt cacttacgtg atctgatatt tcactgtcag
     8101 aatcctcacc aacaagctcg tcatcgcttt gcagaagagc agagaggata tgctcatcgt
     8161 ctaaagaact acccatttta ttatatatta gtcacgatat ctataacaag aaaatatata
     8221 tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa
     8281 atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt
     8341 tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga
     8401 ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt
     8461 tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaaaa aagatttgcg
     8521 ctttactcga cctaaacttt aaacacgtca tagaatcttc gtttgacaaa aaccacattg
     8581 tggccaagct gtgtgacgcg acgcgcgcta aagaatggca aaccaagtcg cgcgagcgtc
     8641 gactctagag gatccccggg taccgagctc gaattcgtaa tcatgtcata gctgtttcct
     8701 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8761 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8821 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8881 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8941 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9001 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9061 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9121 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9181 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9241 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9301 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9361 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9421 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9481 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9541 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9601 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9661 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9721 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9781 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9841 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9901 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9961 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10021 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10081 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10141 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    10201 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    10261 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10321 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10381 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10441 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10501 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10561 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10621 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10681 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10741 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10801 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    10861 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    10921 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
    10981 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
    11041 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    11101 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
    11161 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    11221 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    11281 cgttgtaaaa cgacggccag tgccaagctt tgtttaaaaa tataacaaaa ttgtgatccc
    11341 acaaaatgaa gtggggcaaa atcaaataat taatagtgtc cgtaaacttg ttggtcttca
    11401 actttttgag gaacacgttg gacggcaaat ccgtgactat aacacaagtt gatttaataa
    11461 ttttagccaa cacgtcgggc tgcgtgtttt ttgccgacgc gtctgtgtac acgttgatta
    11521 actggtcgat taaactgttg aaataattta atttttggtt cttctttaaa tctgtgatga
    11581 aattttttaa aataacttta aattcttcat tggtaaaaaa tgccacgttt tgcaacttgt
    11641 gagggtctaa tatgaggtca aactcagtag gagttttatc caaaaaagaa aacatgatta
    11701 cgtctgtaca cgaacgcgta ttaacgcaga gtgcaaagta taagagggtt aaaaaatata
    11761 ttttacgcac catatacgca tcgggttgat atcgttaata tggatcaatt tgaacagttg
    11821 attaacgtgt ctctgctcaa gtctttgatc aaaacgcaaa tcgacgaaaa tgtgtcggac
    11881 aatatcaagt cgatgagcga aaaactaaaa aggctagaat acgacaatct cacagacagc
    11941 gttgagatat acggtattca cgacagcagg ctgaataata aaaaaattag aaactattat
    12001 ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg cgtaaaattg
    12061 acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa tagatattaa
    12121 gttttattat atttacactt acatactaat aataaattca acaaacaatt tatttatgtt
    12181 tatttattta ttaaaaaaaa acaaaaactc aaaatttctt ctataaagta acaaaacttt
    12241 taaacattct ctcttttaca aaaataaact tattttgtac tttaaaaaca gtcatgttgt
    12301 attataaaat aagtaattag cttaacttat acataataga aacaaattat acttattagt
    12361 cagtcagaaa caactttggc acatatcaat attatgctct cgacaaataa cttttttgca
    12421 ttttttgcac gatgcatttg cctttcgcct tattttagag gggcagtaag tacagtaagt
    12481 acgttttttc attactggct cttcagtact gtcatctgat gtaccaggca cttcatttgg
    12541 caaaatatta gagatattat cgcgcaaata tctcttcaaa gtaggagctt ctaaacgctt
    12601 acgcataaac gatgacgtca ggctcatgta aaggtttctc ataaattttt tgcgactttg
    12661 aaccttttct cccttgctac tgacattatg gctgtatata ataaaagaat ttatgcaggc
    12721 aatgtttatc attccgtaca ataatgccat aggccaccta ttcgtcttcc tactgcaggt
    12781 catcacagaa cacatttggt ctagcgtgtc cactccgcct ttagtttgat tataatacat
    12841 aaccatttgc ggtttaccgg tactttcgtt gatagaagca tcctcatcac aagatgataa
    12901 taagtatacc atcttagctg gcttcggttt atatgagacg agagtaaggg gtccgtcaaa
    12961 acaaaacatc gatgttccca ctggcctgga gcgactgttt ttcagtactt ccggtatctc
    13021 gcgtttgttt gatcgcacgg ttcccacaat ggttaattcg agctcgcccg gggtcctagg
    13081 tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc
    13141 tccccgggcg cgtactccac ctcacccatc tggtccatca tgatgaacgg gtcgaggtgg
    13201 cggtagttga tcccggcgaa cgcgcggcgc accgggaagc cctcgccctc gaaaccgctg
    13261 ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt cggcgggtgc ggatacgcgg
    13321 ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt cgacg
//
