LOCUS       sequence-244211-       10698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses gRNAs targeting hid,  eve, and hedgehog.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Buchman A, Shriner I, Yang T, Liu J, Antoshechkin I, Marshall JM, 
            Perry MW, Akbari OS
  TITLE     Engineered reproductively isolated species drive reversible 
            population replacement.
  JOURNAL   Nat Commun. 2021 Jun 2;12(1):3281. doi: 10.1038/s41467-021-23531-z.
  PUBMED    34078888
REFERENCE   2  (bases 1 to 10698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-2"; volume: "12(1):3281. doi"; pages: " 
            10.1038/s41467-021-23531-z"
COMMENT     Sequence Label: OA-1045B
FEATURES             Location/Qualifiers
     source          1..10698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    183..216
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        275..695
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        715..790
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        805..1601
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     misc_RNA        1620..1695
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    1736..2121
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     promoter        2135..2931
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     misc_RNA        2952..3027
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3028..3448
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        3469..3544
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    3613..3682
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(3901..3919)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3940..3956)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3940..3956)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3953..3975)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3964..3980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3988..3994,3995..4012,4013..4018))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4033..4054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4171..4188)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4342..4930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4422..4441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5101..5892,5893..5961))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5724..5743
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5962..6066)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6093..6520
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6180..6199)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6390..6411
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6674..6696
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6688..6705
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6689..6705
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6712..6731
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6712..6730
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6764..6782)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7234..7305,7707..7980,8055..8709,8771..9086,9290..9421,
                     9492..10106)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt tccttgtgta
       61 gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca aaaataacaa
      121 ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg aactaggcta
      181 gcataacttc gtataatgta tgctatacga agttatgcta gcggatccgg gaattgggaa
      241 ttgggcaata tttaaatggc ggcgcgccga attctttttt gctcacctgt gattgctcct
      301 actcaaatac aaaaacatca aattttctgt caataaagca tatttattta tatttatttt
      361 acaggaaaga attcctttta aagtgtattt taacctataa tgaaaaacga ttaaaaaaaa
      421 tacataaaat aattcgaaaa tttttgaata gcccaggttg ataaaaattc atttcatacg
      481 ttttataact tatgccccta agtatttttt gaccatagtg tttcaattct acattaattt
      541 tacagagtag aatgaaacgc cacctactca gccaagaggc gaaaaggtta gctcgccaag
      601 cagagagggc gccagtgctc actacttttt ataattctca acttcttttt ccagactcag
      661 ttcgtatata tagacctatt ttcaatttaa cgtcggatcg tgcggtgctg agaggtttta
      721 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
      781 gagtcggtgc ttttttgttt aaacattttc aacgtcctcg atagtatagt ggttagtatc
      841 cccgcctgtc acgcgggaga ccggggttca attccccgtc ggggagaatc tgtgattctt
      901 tttttttttc ttttactttg ttatataaac aatttttgtt ttaattgaat ctaatttgcc
      961 attgctttta ggaatctcag gcatccagca agcgtttgtc cgccgaatcg cccatcagtg
     1021 aagaagatcc tgtggcggct acgaaaatct ccccggccat gtcggcctcc acctccagcg
     1081 aaaaacccat cagcgagctg gccacctctg tgctgaccca ccgctttcca gactccacct
     1141 cctcacccgg cgaacatggc cttggacgaa tgcagttgtc gatccgctac agcgcccagc
     1201 gtcaaaaact agacgtgacc atacacaaaa tccagaagat accacttcgc gatcccagca
     1261 atatccccga tccgtatgtt aagctgtatc tgttgcctgg acgcaccaag gagtcgaaac
     1321 gcaagacgag cgtgatcaag gacaactgca accccgtcta cgatgcatcc tttgagtacc
     1381 tgatttccat tgccgaactc aggcagacgg aactggaggt gacggtgtgc acccaaaagg
     1441 gattcctatc cggcggtagt cccatcattg gcatggtagg tacccgaaag caacccctta
     1501 gttacagaca cagcgcgtac gtccttcgca tccttatgat tcccaagtac atattctgca
     1561 agagtacagt atatatagga aagatatccg ggtgaacttc ggcacatgca cgtgcatgag
     1621 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     1681 gcaccgagtc ggtgcttttt taattaagga acgcggccgc aacggtacct gcagccacgt
     1741 aataagtgtg cgttgaattt attcgcaaaa acattgcata ttttcggcaa agtaaaattt
     1801 tgttgcatac cttatcaaaa aataagtgct gcatactttt tagagaaacc aaataatttt
     1861 ttattgcata cccgttttta ataaaataca ttgcataccc tcttttaata aaaaatattg
     1921 catactttga cgaaacaaat tttcgttgca tacccaataa aagattatta tattgcatac
     1981 ccgtttttaa taaaatacat tgcataccct cttttaataa agaatattgc atacgttgac
     2041 gaaacaaatt ttcgttgcat acccaataaa agattattat attgcatacc ttttcttgcc
     2101 ataccattta gccgatcaat tgagatctga attcattttc aacgtcctcg atagtatagt
     2161 ggttagtatc cccgcctgtc acgcgggaga ccggggttca attccccgtc ggggagaatc
     2221 tgtgattctt tttttttttc ttttactttg ttatataaac aatttttgtt ttaattgaat
     2281 ctaatttgcc attgctttta ggaatctcag gcatccagca agcgtttgtc cgccgaatcg
     2341 cccatcagtg aagaagatcc tgtggcggct acgaaaatct ccccggccat gtcggcctcc
     2401 acctccagcg aaaaacccat cagcgagctg gccacctctg tgctgaccca ccgctttcca
     2461 gactccacct cctcacccgg cgaacatggc cttggacgaa tgcagttgtc gatccgctac
     2521 agcgcccagc gtcaaaaact agacgtgacc atacacaaaa tccagaagat accacttcgc
     2581 gatcccagca atatccccga tccgtatgtt aagctgtatc tgttgcctgg acgcaccaag
     2641 gagtcgaaac gcaagacgag cgtgatcaag gacaactgca accccgtcta cgatgcatcc
     2701 tttgagtacc tgatttccat tgccgaactc aggcagacgg aactggaggt gacggtgtgc
     2761 acccaaaagg gattcctatc cggcggtagt cccatcattg gcatggtagg tacccgaaag
     2821 caacccctta gttacagaca cagcgcgtac gtccttcgca tccttatgat tcccaagtac
     2881 atattctgca agagtacagt atatatagga aagatatccg ggtgaacttc gtatgccact
     2941 cgacgttcga tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     3001 cttgaaaaag tggcaccgag tcggtgcttt tttgctcacc tgtgattgct cctactcaaa
     3061 tacaaaaaca tcaaattttc tgtcaataaa gcatatttat ttatatttat tttacaggaa
     3121 agaattcctt ttaaagtgta ttttaaccta taatgaaaaa cgattaaaaa aaatacataa
     3181 aataattcga aaatttttga atagcccagg ttgataaaaa ttcatttcat acgttttata
     3241 acttatgccc ctaagtattt tttgaccata gtgtttcaat tctacattaa ttttacagag
     3301 tagaatgaaa cgccacctac tcagccaaga ggcgaaaagg ttagctcgcc aagcagagag
     3361 ggcgccagtg ctcactactt tttataattc tcaacttctt tttccagact cagttcgtat
     3421 atatagacct attttcaatt taacgtcgtt ccacttccct tgcgcatagt tttagagcta
     3481 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     3541 gtgctttttt gcctacctgg agcctgagag ttgttctaga gatccactag tgtcgacgat
     3601 gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg
     3661 cgcgtactcc acctcaccca tctggtccat catgatgaac gggtcgaggt ggcggtagtt
     3721 gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt
     3781 ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt
     3841 cagcgggttc tcgacggtca cggcgggcat gtcgacacta gttctagcca gcttttgttc
     3901 cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     3961 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     4021 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     4081 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     4141 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4201 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4261 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4321 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4381 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4441 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4501 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4561 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4621 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4681 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4741 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     4801 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4861 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4921 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4981 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5041 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5101 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5161 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5221 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5281 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5341 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5401 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5461 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5521 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5581 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5641 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5701 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5761 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5821 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5881 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5941 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     6001 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     6061 tccgcgcaca tttcscckra awgkssmays kgacgcgccc tgtagcggcg cattaagcgc
     6121 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6181 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6241 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6301 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6361 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6421 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6481 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat ykywwmawrm kmykmwmsag
     6541 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     6601 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     6661 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac
     6721 tcactatagg gcgaattggg tacgtaccgg gcccctagta tgtatgtaag ttaataaaac
     6781 ccatttttgc ggaaagtaga taaaaaaaac attttttttt tttactgcac tggatatcat
     6841 tgaacttatc tgatcagttt taaatttact tcgatccaag ggtatttgat gtaccaggtt
     6901 ctttcgatta cctctcactc aaaatgacat tccactcaaa gtcagcgctg tttgcctcct
     6961 tctctgtcca cagaaatatc gccgtctctt tcgccgctgc gtccgctatc tctttcgcca
     7021 ccgtttgtag cgttacgtag cgtcaatgtc cgccttcagt tgcattttgt cagcggtttc
     7081 gtgacgaagc tccaagcggt ttacgccatc aattaaacac aaagtgctgt gccaaaactc
     7141 ctctcgcttc ttatttttgt ttgttttttg agtgattggg gtggtgattg gttttgggtg
     7201 ggtaagcagg ggaaagtgtg aaaaatcccg gcaatgggcc aagaggatca ggagctatta
     7261 attcgcggag gcagcaaaca cccatctgcc gagcatctga acaatgtgag tagtacatgt
     7321 gcatacatct taagttcact tgatctatag gaactgcgat tgcaacatca aattgtctgc
     7381 ggcgtgagaa ctgcgaccca caaaaatccc aaaccgcaat tgcacaaaca aatagtgaca
     7441 cgaaacagat tattctggta gctgttctcg ctatataaga caatttttga gatcatatca
     7501 tgatcaagac atctaaaggc attcattttc gactatattc ttttttacaa aaaatataac
     7561 aaccagatat tttaagctga tcctagatgc acaaaaaata aataaaagta taaacctact
     7621 tcgtaggata cttcggggta ctttttgttc ggggttagat gagcataacg cttgtagttg
     7681 atatttgaga tcccctatca ttgcagggtg acagcggagc ggcttcgcag agctgcatta
     7741 accagggctt cgggcaggcc aaaaactacg gcacgctccg gccacccagt ccgccggagg
     7801 actccggttc agggagcggc caactagccg agaacctcac ctatgcctgg cacaatatgg
     7861 acatctttgg ggcggtcaat cagccgggct ccggatggcg gcagctggtc aaccggacac
     7921 gcggactatt ctgcaacgag cgacacatac cggcgcccag gaaacatttg ctcaagaacg
     7981 gtgagtttct attcgcagtc ggctgatctg tgtgaaatct taataaaggg tccaattacc
     8041 aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa cttttggccg tgatgggcag
     8101 ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat
     8161 ccaagtatcg ccatccggga tgcgactgct caatggccaa cctgtggacg ccaaggagat
     8221 gcaggccagg tgcgcctatg tccagcagga tgacctcttt atcggctccc taacggccag
     8281 ggaacacctg attttccaag ccatggtgcg gatgccacga catctgacct atcggcagcg
     8341 agtggcccgc gtggatcagg tgatccagga gctttcgctc agcaaatgtc agcacacgat
     8401 catcggtgtg cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt
     8461 cgcctccgag gctctaaccg atccgccgct tctgatctgc gatgagccca cctccggact
     8521 ggactccttt accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa
     8581 gaccgtcatc ctgaccattc atcagccgtc ttccgagctg tttgagctct ttgacaagat
     8641 ccttctgatg gccgagggca gggtagcttt cttgggcact cccagcgaag ccgtcgactt
     8701 cttttcctag tgagttcgat gtgtttatta agggtatcta gtattacata acatctcaac
     8761 tcctatccag cgtgggtgcc cagtgtccta ccaactacaa tccggcggac ttttacgtac
     8821 aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc gccaagatat
     8881 gcgacaattt tgccattagc aaagtagccc gggatatgga gcagttgttg gccaccaaaa
     8941 atctggagaa gccactggag cagccggaga atgggtacac ctacaaggcc acctggttca
     9001 tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg
     9061 taaaagtgcg acttattcag acaacggtga gtggttccag tggaaacaaa tgatataacg
     9121 cttacaattc ttggaaacaa attcgctaga ttttagatag aattgcctga ttccacaccc
     9181 ttcttagttt ttttcaatga gatgtatagt ttatagtttt gcagaagata aataaatttc
     9241 atttaactcg cgaatattaa tgagatgcga gtaacatttt aatttgcaga tggttgccat
     9301 cttgattggc ctcatctttt tgggccaaca actcacgcaa gtgggtgtga tgaatatcaa
     9361 cggagccatc ttcctcttcc tgaccaacat gacctttcaa aacgtctttg ccacgataaa
     9421 tgtaagtcat gtttagaata catttgcatt tcaataattt actaactttc taatgaatcg
     9481 attcgattta ggtgttcacc tcagagctgc cagtttttat gagggaggcc cgaagtcgac
     9541 tttatcgctg tgacacatac tttctgggca aaacgattgc cgaattgccg ctttttctca
     9601 cagtgccact ggtcttcacg gcgattgcct atccgatgat cggactgcgg gccggagtgc
     9661 tgcacttctt caactgcctg gcgctggtca ctctggtggc caatgtgtca acgtccttcg
     9721 gatatctaat atcctgcgcc agctcctcga cctcgatggc gctgtctgtg ggtccgccgg
     9781 ttatcatacc attcctgctc tttggcggct tcttcttgaa ctcgggctcg gtgccagtat
     9841 acctcaaatg gttgtcgtac ctctcatggt tccgttacgc caacgagggt ctgctgatta
     9901 accaatgggc ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc
     9961 ccagttcggg caaggtcatc ctggagacgc ttaacttctc cgccgccgat ctgccgctgg
    10021 actacgtggg tctggccatt ctcatcgtga gcttccgggt gctcgcatat ctggctctaa
    10081 gacttcgggc ccgacgcaag gagtagccga catatatccg aaataactgc ttgttttttt
    10141 tttttaccat tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat
    10201 tatatttgtg ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa
    10261 catccccaca agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac
    10321 cttacatcaa aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat
    10381 acttttcaaa tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg
    10441 ctctttcgct gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg
    10501 gagcgactgt cgttagttcc gcgcgattcg gttcgctcaa atggttccga gtggttcatt
    10561 tcgtctcaat agaaattagt aataaatatt tgtatgtaca atttatttgc tccaatatat
    10621 ttgtatatat ttccctcaca gctatattta ttctaattta atattatgac tttttaaggt
    10681 aattttttgt gacctgtt
//
