LOCUS       sequence-244215-       10820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses gRNAs targeting hid, eve, and wingless.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10820)
  AUTHORS   Buchman A, Shriner I, Yang T, Liu J, Antoshechkin I, Marshall JM, 
            Perry MW, Akbari OS
  TITLE     Engineered reproductively isolated species drive reversible 
            population replacement.
  JOURNAL   Nat Commun. 2021 Jun 2;12(1):3281. doi: 10.1038/s41467-021-23531-z.
  PUBMED    34078888
REFERENCE   2  (bases 1 to 10820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-2"; volume: "12(1):3281. doi"; pages: " 
            10.1038/s41467-021-23531-z"
COMMENT     Sequence Label: OA-1045C
FEATURES             Location/Qualifiers
     source          1..10820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     751..770
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        751..769
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(803..821)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1273..1344,1746..2019,2094..2748,2810..3125,3329..3460,
                     3531..4145)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4920..4953
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5012..5432
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        5452..5527
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        5542..6338
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     misc_RNA        6357..6432
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    6473..6858
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     promoter        6872..7668
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     misc_RNA        7689..7764
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        7765..8185
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        8205..8280
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8349..8418
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(8637..8655)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8676..8692)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8676..8692)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8689..8711)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8700..8716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8724..8730,8731..8748,8749..8754))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8769..8790
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8907..8924)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9078..9666)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9158..9177)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9837..10628,10629..10697))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10460..10479
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10698..10802)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt
      781 acgtaccggg cccctagtat gtatgtaagt taataaaacc catttttgcg gaaagtagat
      841 aaaaaaaaca tttttttttt ttactgcact ggatatcatt gaacttatct gatcagtttt
      901 aaatttactt cgatccaagg gtatttgatg taccaggttc tttcgattac ctctcactca
      961 aaatgacatt ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac agaaatatcg
     1021 ccgtctcttt cgccgctgcg tccgctatct ctttcgccac cgtttgtagc gttacgtagc
     1081 gtcaatgtcc gccttcagtt gcattttgtc agcggtttcg tgacgaagct ccaagcggtt
     1141 tacgccatca attaaacaca aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt
     1201 tgttttttga gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga
     1261 aaaatcccgg caatgggcca agaggatcag gagctattaa ttcgcggagg cagcaaacac
     1321 ccatctgccg agcatctgaa caatgtgagt agtacatgtg catacatctt aagttcactt
     1381 gatctatagg aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac
     1441 aaaaatccca aaccgcaatt gcacaaacaa atagtgacac gaaacagatt attctggtag
     1501 ctgttctcgc tatataagac aatttttgag atcatatcat gatcaagaca tctaaaggca
     1561 ttcattttcg actatattct tttttacaaa aaatataaca accagatatt ttaagctgat
     1621 cctagatgca caaaaaataa ataaaagtat aaacctactt cgtaggatac ttcggggtac
     1681 tttttgttcg gggttagatg agcataacgc ttgtagttga tatttgagat cccctatcat
     1741 tgcagggtga cagcggagcg gcttcgcaga gctgcattaa ccagggcttc gggcaggcca
     1801 aaaactacgg cacgctccgg ccacccagtc cgccggagga ctccggttca gggagcggcc
     1861 aactagccga gaacctcacc tatgcctggc acaatatgga catctttggg gcggtcaatc
     1921 agccgggctc cggatggcgg cagctggtca accggacacg cggactattc tgcaacgagc
     1981 gacacatacc ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta ttcgcagtcg
     2041 gctgatctgt gtgaaatctt aataaagggt ccaattacca atttgaaact cagtttgcgg
     2101 cgtggcctat ccgggcgaac ttttggccgt gatgggcagt tccggtgccg gaaagacgac
     2161 cctgctgaat gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc catccgggat
     2221 gcgactgctc aatggccaac ctgtggacgc caaggagatg caggccaggt gcgcctatgt
     2281 ccagcaggat gacctcttta tcggctccct aacggccagg gaacacctga ttttccaagc
     2341 catggtgcgg atgccacgac atctgaccta tcggcagcga gtggcccgcg tggatcaggt
     2401 gatccaggag ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt
     2461 gaaaggtctg tccggcggag aaaggaagcg tctggcattc gcctccgagg ctctaaccga
     2521 tccgccgctt ctgatctgcg atgagcccac ctccggactg gactccttta ccgcccacag
     2581 cgtcgtccag gtgctgaaga agctgtcgca gaagggcaag accgtcatcc tgaccattca
     2641 tcagccgtct tccgagctgt ttgagctctt tgacaagatc cttctgatgg ccgagggcag
     2701 ggtagctttc ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt gagttcgatg
     2761 tgtttattaa gggtatctag tattacataa catctcaact cctatccagc gtgggtgccc
     2821 agtgtcctac caactacaat ccggcggact tttacgtaca ggtgttggcc gttgtgcccg
     2881 gacgggagat cgagtcccgt gatcggatcg ccaagatatg cgacaatttt gccattagca
     2941 aagtagcccg ggatatggag cagttgttgg ccaccaaaaa tctggagaag ccactggagc
     3001 agccggagaa tgggtacacc tacaaggcca cctggttcat gcagttccgg gcggtcctgt
     3061 ggcgatcctg gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga cttattcaga
     3121 caacggtgag tggttccagt ggaaacaaat gatataacgc ttacaattct tggaaacaaa
     3181 ttcgctagat tttagataga attgcctgat tccacaccct tcttagtttt tttcaatgag
     3241 atgtatagtt tatagttttg cagaagataa ataaatttca tttaactcgc gaatattaat
     3301 gagatgcgag taacatttta atttgcagat ggttgccatc ttgattggcc tcatcttttt
     3361 gggccaacaa ctcacgcaag tgggtgtgat gaatatcaac ggagccatct tcctcttcct
     3421 gaccaacatg acctttcaaa acgtctttgc cacgataaat gtaagtcatg tttagaatac
     3481 atttgcattt caataattta ctaactttct aatgaatcga ttcgatttag gtgttcacct
     3541 cagagctgcc agtttttatg agggaggccc gaagtcgact ttatcgctgt gacacatact
     3601 ttctgggcaa aacgattgcc gaattgccgc tttttctcac agtgccactg gtcttcacgg
     3661 cgattgccta tccgatgatc ggactgcggg ccggagtgct gcacttcttc aactgcctgg
     3721 cgctggtcac tctggtggcc aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca
     3781 gctcctcgac ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca ttcctgctct
     3841 ttggcggctt cttcttgaac tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc
     3901 tctcatggtt ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg gacgtggagc
     3961 cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc
     4021 tggagacgct taacttctcc gccgccgatc tgccgctgga ctacgtgggt ctggccattc
     4081 tcatcgtgag cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg
     4141 agtagccgac atatatccga aataactgct tgtttttttt ttttaccatt attaccatcg
     4201 tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac
     4261 aagatatgac ctatagaata caagtatttc cccttcgaac atccccacaa gtagactttg
     4321 gatttgtctt ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa aactcgttta
     4381 tcgctacata aaacaccggg atatattttt tatatacata cttttcaaat cgcgcgccct
     4441 cttcataatt cacctccacc acaccacgtt tcgtagttgc tctttcgctg tctcccaccc
     4501 gctctccgca acacattcac cttttgttcg acgaccttgg agcgactgtc gttagttccg
     4561 cgcgattcgg ttcgctcaaa tggttccgag tggttcattt cgtctcaata gaaattagta
     4621 ataaatattt gtatgtacaa tttatttgct ccaatatatt tgtatatatt tccctcacag
     4681 ctatatttat tctaatttaa tattatgact ttttaaggta attttttgtg acctgttcgg
     4741 agtgattagc gttacaattt gaactgaaag tgacatccag tgtttgttcc ttgtgtagat
     4801 gcatctcaaa aaaatggtgg gcataatagt gttgtttata tatatcaaaa ataacaacta
     4861 taataataag aatacattta atttagaaaa tgcttggatt tcactggaac taggctagca
     4921 taacttcgta taatgtatgc tatacgaagt tatgctagcg gatccgggaa ttgggaattg
     4981 ggcaatattt aaatggcggc gcgccgaatt cttttttgct cacctgtgat tgctcctact
     5041 caaatacaaa aacatcaaat tttctgtcaa taaagcatat ttatttatat ttattttaca
     5101 ggaaagaatt ccttttaaag tgtattttaa cctataatga aaaacgatta aaaaaaatac
     5161 ataaaataat tcgaaaattt ttgaatagcc caggttgata aaaattcatt tcatacgttt
     5221 tataacttat gcccctaagt attttttgac catagtgttt caattctaca ttaattttac
     5281 agagtagaat gaaacgccac ctactcagcc aagaggcgaa aaggttagct cgccaagcag
     5341 agagggcgcc agtgctcact actttttata attctcaact tctttttcca gactcagttc
     5401 gtatatatag acctattttc aatttaacgt cggatcgtgc ggtgctgaga ggttttagag
     5461 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     5521 tcggtgcttt tttgtttaaa cattttcaac gtcctcgata gtatagtggt tagtatcccc
     5581 gcctgtcacg cgggagaccg gggttcaatt ccccgtcggg gagaatctgt gattcttttt
     5641 ttttttcttt tactttgtta tataaacaat ttttgtttta attgaatcta atttgccatt
     5701 gcttttagga atctcaggca tccagcaagc gtttgtccgc cgaatcgccc atcagtgaag
     5761 aagatcctgt ggcggctacg aaaatctccc cggccatgtc ggcctccacc tccagcgaaa
     5821 aacccatcag cgagctggcc acctctgtgc tgacccaccg ctttccagac tccacctcct
     5881 cacccggcga acatggcctt ggacgaatgc agttgtcgat ccgctacagc gcccagcgtc
     5941 aaaaactaga cgtgaccata cacaaaatcc agaagatacc acttcgcgat cccagcaata
     6001 tccccgatcc gtatgttaag ctgtatctgt tgcctggacg caccaaggag tcgaaacgca
     6061 agacgagcgt gatcaaggac aactgcaacc ccgtctacga tgcatccttt gagtacctga
     6121 tttccattgc cgaactcagg cagacggaac tggaggtgac ggtgtgcacc caaaagggat
     6181 tcctatccgg cggtagtccc atcattggca tggtaggtac ccgaaagcaa ccccttagtt
     6241 acagacacag cgcgtacgtc cttcgcatcc ttatgattcc caagtacata ttctgcaaga
     6301 gtacagtata tataggaaag atatccgggt gaacttcggc acatgcacgt gcatgagttt
     6361 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
     6421 ccgagtcggt gcttttttaa ttaaggaacg cggccgcaac ggtacctgca gccacgtaat
     6481 aagtgtgcgt tgaatttatt cgcaaaaaca ttgcatattt tcggcaaagt aaaattttgt
     6541 tgcatacctt atcaaaaaat aagtgctgca tactttttag agaaaccaaa taatttttta
     6601 ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa aatattgcat
     6661 actttgacga aacaaatttt cgttgcatac ccaataaaag attattatat tgcatacccg
     6721 tttttaataa aatacattgc ataccctctt ttaataaaga atattgcata cgttgacgaa
     6781 acaaattttc gttgcatacc caataaaaga ttattatatt gcataccttt tcttgccata
     6841 ccatttagcc gatcaattga gatctgaatt cattttcaac gtcctcgata gtatagtggt
     6901 tagtatcccc gcctgtcacg cgggagaccg gggttcaatt ccccgtcggg gagaatctgt
     6961 gattcttttt ttttttcttt tactttgtta tataaacaat ttttgtttta attgaatcta
     7021 atttgccatt gcttttagga atctcaggca tccagcaagc gtttgtccgc cgaatcgccc
     7081 atcagtgaag aagatcctgt ggcggctacg aaaatctccc cggccatgtc ggcctccacc
     7141 tccagcgaaa aacccatcag cgagctggcc acctctgtgc tgacccaccg ctttccagac
     7201 tccacctcct cacccggcga acatggcctt ggacgaatgc agttgtcgat ccgctacagc
     7261 gcccagcgtc aaaaactaga cgtgaccata cacaaaatcc agaagatacc acttcgcgat
     7321 cccagcaata tccccgatcc gtatgttaag ctgtatctgt tgcctggacg caccaaggag
     7381 tcgaaacgca agacgagcgt gatcaaggac aactgcaacc ccgtctacga tgcatccttt
     7441 gagtacctga tttccattgc cgaactcagg cagacggaac tggaggtgac ggtgtgcacc
     7501 caaaagggat tcctatccgg cggtagtccc atcattggca tggtaggtac ccgaaagcaa
     7561 ccccttagtt acagacacag cgcgtacgtc cttcgcatcc ttatgattcc caagtacata
     7621 ttctgcaaga gtacagtata tataggaaag atatccgggt gaacttcgat gaggttgcgc
     7681 aaataatcgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
     7741 gaaaaagtgg caccgagtcg gtgctttttt gctcacctgt gattgctcct actcaaatac
     7801 aaaaacatca aattttctgt caataaagca tatttattta tatttatttt acaggaaaga
     7861 attcctttta aagtgtattt taacctataa tgaaaaacga ttaaaaaaaa tacataaaat
     7921 aattcgaaaa tttttgaata gcccaggttg ataaaaattc atttcatacg ttttataact
     7981 tatgccccta agtatttttt gaccatagtg tttcaattct acattaattt tacagagtag
     8041 aatgaaacgc cacctactca gccaagaggc gaaaaggtta gctcgccaag cagagagggc
     8101 gccagtgctc actacttttt ataattctca acttcttttt ccagactcag ttcgtatata
     8161 tagacctatt ttcaatttaa cgtcggaaat ggaaaaactc tgccgtttta gagctagaaa
     8221 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     8281 ttttttgcct acctggagcc tgagagttgt tctagagatc cactagtgtc gacgatgtag
     8341 gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg
     8401 tactccacct cacccatctg gtccatcatg atgaacgggt cgaggtggcg gtagttgatc
     8461 ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga aaccgctggg cgcggtggtc
     8521 acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg atacgcgggg cagcgtcagc
     8581 gggttctcga cggtcacggc gggcatgtcg acactagttc tagccagctt ttgttccctt
     8641 tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     8701 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     8761 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     8821 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8881 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8941 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9001 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9061 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9121 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9181 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9241 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9301 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9361 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9421 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9481 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9541 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9601 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9661 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9721 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9781 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9841 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9901 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9961 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10021 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10081 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10141 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10201 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10261 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10321 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10381 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10441 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10501 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10561 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10621 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10681 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10741 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10801 cgsamatkty smmkactcat
//
