LOCUS       sequence-244238-       10776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses gRNAs targeting hedgehog and wingless.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10776)
  AUTHORS   Buchman A, Shriner I, Yang T, Liu J, Antoshechkin I, Marshall JM, 
            Perry MW, Akbari OS
  TITLE     Engineered reproductively isolated species drive reversible 
            population replacement.
  JOURNAL   Nat Commun. 2021 Jun 2;12(1):3281. doi: 10.1038/s41467-021-23531-z.
  PUBMED    34078888
REFERENCE   2  (bases 1 to 10776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-2"; volume: "12(1):3281. doi"; pages: " 
            10.1038/s41467-021-23531-z"
COMMENT     Sequence Label: OA-1045D
FEATURES             Location/Qualifiers
     source          1..10776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1751..2206)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1888..1909)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2100..2119
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2407..2429
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2421..2438
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2422..2438
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2445..2464
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2445..2463
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2497..2515)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2967..3038,3440..3713,3788..4442,4504..4819,5023..5154,
                     5225..5839)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    6614..6647
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6725..7521
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     misc_RNA        7542..7617
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        7618..8038
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        8058..8133
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8171..8556
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     promoter        8570..9366
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     misc_RNA        9387..9462
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        9463..9883
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        9904..9979
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    10048..10117
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(10336..10354)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10375..10391)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10375..10391)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10388..10410)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10399..10415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10423..10429,10430..10447,10448..10453))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10468..10489
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10606..10623)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     1801 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     1861 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     1921 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     1981 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     2041 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     2101 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     2161 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     2221 aggcygcgcw rcksktrrsm assrcrcccg ccgcgcttaa tgcgccgcta cagggcgcgt
     2281 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     2341 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     2401 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
     2461 agggcgaatt gggtacgtac cgggccccta gtatgtatgt aagttaataa aacccatttt
     2521 tgcggaaagt agataaaaaa aacatttttt ttttttactg cactggatat cattgaactt
     2581 atctgatcag ttttaaattt acttcgatcc aagggtattt gatgtaccag gttctttcga
     2641 ttacctctca ctcaaaatga cattccactc aaagtcagcg ctgtttgcct ccttctctgt
     2701 ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg
     2761 tagcgttacg tagcgtcaat gtccgccttc agttgcattt tgtcagcggt ttcgtgacga
     2821 agctccaagc ggtttacgcc atcaattaaa cacaaagtgc tgtgccaaaa ctcctctcgc
     2881 ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga ttggttttgg gtgggtaagc
     2941 aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga tcaggagcta ttaattcgcg
     3001 gaggcagcaa acacccatct gccgagcatc tgaacaatgt gagtagtaca tgtgcataca
     3061 tcttaagttc acttgatcta taggaactgc gattgcaaca tcaaattgtc tgcggcgtga
     3121 gaactgcgac ccacaaaaat cccaaaccgc aattgcacaa acaaatagtg acacgaaaca
     3181 gattattctg gtagctgttc tcgctatata agacaatttt tgagatcata tcatgatcaa
     3241 gacatctaaa ggcattcatt ttcgactata ttctttttta caaaaaatat aacaaccaga
     3301 tattttaagc tgatcctaga tgcacaaaaa ataaataaaa gtataaacct acttcgtagg
     3361 atacttcggg gtactttttg ttcggggtta gatgagcata acgcttgtag ttgatatttg
     3421 agatccccta tcattgcagg gtgacagcgg agcggcttcg cagagctgca ttaaccaggg
     3481 cttcgggcag gccaaaaact acggcacgct ccggccaccc agtccgccgg aggactccgg
     3541 ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt
     3601 tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact
     3661 attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga acggtgagtt
     3721 tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt accaatttga
     3781 aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg cagttccggt
     3841 gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg catccaagta
     3901 tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga gatgcaggcc
     3961 aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc cagggaacac
     4021 ctgattttcc aagccatggt gcggatgcca cgacatctga cctatcggca gcgagtggcc
     4081 cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac gatcatcggt
     4141 gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc attcgcctcc
     4201 gaggctctaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg actggactcc
     4261 tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc
     4321 atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa gatccttctg
     4381 atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga cttcttttcc
     4441 tagtgagttc gatgtgttta ttaagggtat ctagtattac ataacatctc aactcctatc
     4501 cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg tacaggtgtt
     4561 ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa
     4621 ttttgccatt agcaaagtag cccgggatat ggagcagttg ttggccacca aaaatctgga
     4681 gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt tcatgcagtt
     4741 ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt
     4801 gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata acgcttacaa
     4861 ttcttggaaa caaattcgct agattttaga tagaattgcc tgattccaca cccttcttag
     4921 tttttttcaa tgagatgtat agtttatagt tttgcagaag ataaataaat ttcatttaac
     4981 tcgcgaatat taatgagatg cgagtaacat tttaatttgc agatggttgc catcttgatt
     5041 ggcctcatct ttttgggcca acaactcacg caagtgggtg tgatgaatat caacggagcc
     5101 atcttcctct tcctgaccaa catgaccttt caaaacgtct ttgccacgat aaatgtaagt
     5161 catgtttaga atacatttgc atttcaataa tttactaact ttctaatgaa tcgattcgat
     5221 ttaggtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc gactttatcg
     5281 ctgtgacaca tactttctgg gcaaaacgat tgccgaattg ccgctttttc tcacagtgcc
     5341 actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag tgctgcactt
     5401 cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct tcggatatct
     5461 aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc cggttatcat
     5521 accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag tatacctcaa
     5581 atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg
     5641 ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc
     5701 gggcaaggtc atcctggaga cgcttaactt ctccgccgcc gatctgccgc tggactacgt
     5761 gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg
     5821 ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt ttttttttac
     5881 cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt aattatattt
     5941 gtgccaataa aaacaagata tgacctatag aatacaagta tttccccttc gaacatcccc
     6001 acaagtagac tttggatttg tcttctaacc aaaagactta cacacctgca taccttacat
     6061 caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata catacttttc
     6121 aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag ttgctctttc
     6181 gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc ttggagcgac
     6241 tgtcgttagt tccgcgcgat tcggttcgct caaatggttc cgagtggttc atttcgtctc
     6301 aatagaaatt agtaataaat atttgtatgt acaatttatt tgctccaata tatttgtata
     6361 tatttccctc acagctatat ttattctaat ttaatattat gactttttaa ggtaattttt
     6421 tgtgacctgt tcggagtgat tagcgttaca atttgaactg aaagtgacat ccagtgtttg
     6481 ttccttgtgt agatgcatct caaaaaaatg gtgggcataa tagtgttgtt tatatatatc
     6541 aaaaataaca actataataa taagaataca tttaatttag aaaatgcttg gatttcactg
     6601 gaactaggct agcataactt cgtataatgt atgctatacg aagttatgct agcggatccg
     6661 ggaattggga attgggcaat atttaaatgg cggcgcgcca gccgatcaat tgagatctga
     6721 attcattttc aacgtcctcg atagtatagt ggttagtatc cccgcctgtc acgcgggaga
     6781 ccggggttca attccccgtc ggggagaatc tgtgattctt tttttttttc ttttactttg
     6841 ttatataaac aatttttgtt ttaattgaat ctaatttgcc attgctttta ggaatctcag
     6901 gcatccagca agcgtttgtc cgccgaatcg cccatcagtg aagaagatcc tgtggcggct
     6961 acgaaaatct ccccggccat gtcggcctcc acctccagcg aaaaacccat cagcgagctg
     7021 gccacctctg tgctgaccca ccgctttcca gactccacct cctcacccgg cgaacatggc
     7081 cttggacgaa tgcagttgtc gatccgctac agcgcccagc gtcaaaaact agacgtgacc
     7141 atacacaaaa tccagaagat accacttcgc gatcccagca atatccccga tccgtatgtt
     7201 aagctgtatc tgttgcctgg acgcaccaag gagtcgaaac gcaagacgag cgtgatcaag
     7261 gacaactgca accccgtcta cgatgcatcc tttgagtacc tgatttccat tgccgaactc
     7321 aggcagacgg aactggaggt gacggtgtgc acccaaaagg gattcctatc cggcggtagt
     7381 cccatcattg gcatggtagg tacccgaaag caacccctta gttacagaca cagcgcgtac
     7441 gtccttcgca tccttatgat tcccaagtac atattctgca agagtacagt atatatagga
     7501 aagatatccg ggtgaacttc gatgaggttg cgcaaataat cgttttagag ctagaaatag
     7561 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     7621 tttgctcacc tgtgattgct cctactcaaa tacaaaaaca tcaaattttc tgtcaataaa
     7681 gcatatttat ttatatttat tttacaggaa agaattcctt ttaaagtgta ttttaaccta
     7741 taatgaaaaa cgattaaaaa aaatacataa aataattcga aaatttttga atagcccagg
     7801 ttgataaaaa ttcatttcat acgttttata acttatgccc ctaagtattt tttgaccata
     7861 gtgtttcaat tctacattaa ttttacagag tagaatgaaa cgccacctac tcagccaaga
     7921 ggcgaaaagg ttagctcgcc aagcagagag ggcgccagtg ctcactactt tttataattc
     7981 tcaacttctt tttccagact cagttcgtat atatagacct attttcaatt taacgtcgga
     8041 aatggaaaaa ctctgccgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
     8101 tatcaacttg aaaaagtggc accgagtcgg tgcttttttg cctacctgga gcctgagagt
     8161 tgttcatatg cacgtaataa gtgtgcgttg aatttattcg caaaaacatt gcatattttc
     8221 ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata ctttttagag
     8281 aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca taccctcttt
     8341 taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc aataaaagat
     8401 tattatattg catacccgtt tttaataaaa tacattgcat accctctttt aataaagaat
     8461 attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc
     8521 ataccttttc ttgccatacc atttagccga tcaattgaga tctgaattca ttttcaacgt
     8581 cctcgatagt atagtggtta gtatccccgc ctgtcacgcg ggagaccggg gttcaattcc
     8641 ccgtcgggga gaatctgtga ttcttttttt ttttctttta ctttgttata taaacaattt
     8701 ttgttttaat tgaatctaat ttgccattgc ttttaggaat ctcaggcatc cagcaagcgt
     8761 ttgtccgccg aatcgcccat cagtgaagaa gatcctgtgg cggctacgaa aatctccccg
     8821 gccatgtcgg cctccacctc cagcgaaaaa cccatcagcg agctggccac ctctgtgctg
     8881 acccaccgct ttccagactc cacctcctca cccggcgaac atggccttgg acgaatgcag
     8941 ttgtcgatcc gctacagcgc ccagcgtcaa aaactagacg tgaccataca caaaatccag
     9001 aagataccac ttcgcgatcc cagcaatatc cccgatccgt atgttaagct gtatctgttg
     9061 cctggacgca ccaaggagtc gaaacgcaag acgagcgtga tcaaggacaa ctgcaacccc
     9121 gtctacgatg catcctttga gtacctgatt tccattgccg aactcaggca gacggaactg
     9181 gaggtgacgg tgtgcaccca aaagggattc ctatccggcg gtagtcccat cattggcatg
     9241 gtaggtaccc gaaagcaacc ccttagttac agacacagcg cgtacgtcct tcgcatcctt
     9301 atgattccca agtacatatt ctgcaagagt acagtatata taggaaagat atccgggtga
     9361 acttcgtatg ccactcgacg ttcgatgttt tagagctaga aatagcaagt taaaataagg
     9421 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgc tcacctgtga
     9481 ttgctcctac tcaaatacaa aaacatcaaa ttttctgtca ataaagcata tttatttata
     9541 tttattttac aggaaagaat tccttttaaa gtgtatttta acctataatg aaaaacgatt
     9601 aaaaaaaata cataaaataa ttcgaaaatt tttgaatagc ccaggttgat aaaaattcat
     9661 ttcatacgtt ttataactta tgcccctaag tattttttga ccatagtgtt tcaattctac
     9721 attaatttta cagagtagaa tgaaacgcca cctactcagc caagaggcga aaaggttagc
     9781 tcgccaagca gagagggcgc cagtgctcac tactttttat aattctcaac ttctttttcc
     9841 agactcagtt cgtatatata gacctatttt caatttaacg tcgttccact tcccttgcgc
     9901 atagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     9961 agtggcaccg agtcggtgct tttttgccta cctggagcct gagagttgtt ctagagatcc
    10021 actagtgtcg acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc
    10081 cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga tgaacgggtc
    10141 gaggtggcgg tagttgatcc cggcgaacgc gcggcgcacc gggaagccct cgccctcgaa
    10201 accgctgggc gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg cgggtgcgga
    10261 tacgcggggc agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga cactagttct
    10321 agccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
    10381 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    10441 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    10501 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    10561 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    10621 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    10681 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    10741 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtt
//
