LOCUS       sequence-244292-       10579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes N. meningiditis dCas9 with c-terminal fusion of human p300 
            HAT core (aa 1048-1664) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA3.3-Nm-dCas9-p300 Core
FEATURES             Location/Qualifiers
     source          1..10579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      693..702
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(699..743,744..746,747..2456,2457..2462,2463..2528,
                     2529..2531,2532..3944)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Neisseria meningitidis Type II 
                     CRISPR/Cas system"
                     /label=NmdCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D16A, D587A, 
                     H588A, and N611A"
                     /translation="MAAFKPNPINYILGLAIGIASVGWAMVEIDEDENPICLIDLGVRV
                     FERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGLIK
                     SLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLKGVA
                     DNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEKQKEF
                     GNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAERFIWLT
                     KLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKGLRYGKD
                     NAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTDEDITGRL
                     KDRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYGDHYGKKNT
                     EEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETAREVGKSFKDRK
                     EIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKCLYSGKEINLG
                     RLNEKGYVEIAAALPFSRTWDDSFNNKVLVLGSEAQNKGNQTPYEYFNGKDNSREWQEF
                     KARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVADRMRLTGKGKKR
                     VFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQKITRFVRYKEMNAF
                     DGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFEEADTPEKLRTLLAE
                     KLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLKLKD
                     LEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKAVRVEQVQK
                     TGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKGILPDRAVVQGKDEEDWQ
                     LIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNINIRIHDLDHKIGKNGILE
                     GIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     regulatory      3531..3540
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3957..3977
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3996..5846
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      5610..5619
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5847..5873
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(5916..5935)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5960..6008
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6210..6638
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6297..6316)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6507..6528
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6647..6667)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6652..6981
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6832..6967
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6894..6913
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7048..7842
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7102..7121)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7712..7731
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8018..8139
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8055..8074)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8109..8128
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8188..8204)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8188..8204)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8201..8223)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8212..8228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8236..8242,8243..8260,8261..8266))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8281..8302
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8419..8436)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8590..9178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8670..8689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9349..10140,10141..10209))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9972..9991
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10210..10314)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10389..10408)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttgacagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gactctagag gatcgaaccc ttgccaccat ggccgccttc aagcccaacc
      721 ccatcaacta catcctgggc ctggccatcg gcatcgccag cgtgggctgg gccatggtgg
      781 agatcgacga ggacgagaac cccatctgcc tgatcgacct gggtgtgcgc gtgttcgagc
      841 gcgctgaggt gcccaagact ggtgacagtc tggctatggc tcgccggctt gctcgctctg
      901 ttcggcgcct tactcgccgg cgcgctcacc gccttctgcg cgctcgccgc ctgctgaagc
      961 gcgagggtgt gctgcaggct gccgacttcg acgagaacgg cctgatcaag agcctgccca
     1021 acactccttg gcagctgcgc gctgccgctc tggaccgcaa gctgactcct ctggagtgga
     1081 gcgccgtgct gctgcacctg atcaagcacc gcggctacct gagccagcgc aagaacgagg
     1141 gcgagaccgc cgacaaggag ctgggtgctc tgctgaaggg cgtggccgac aacgcccacg
     1201 ccctgcagac tggtgacttc cgcactcctg ctgagctggc cctgaacaag ttcgagaagg
     1261 agagcggcca catccgcaac cagcgcggcg actacagcca caccttcagc cgcaaggacc
     1321 tgcaggccga gctgatcctg ctgttcgaga agcagaagga gttcggcaac ccccacgtga
     1381 gcggcggcct gaaggagggc atcgagaccc tgctgatgac ccagcgcccc gccctgagcg
     1441 gcgacgccgt gcagaagatg ctgggccact gcaccttcga gccagccgag cccaaggccg
     1501 ccaagaacac ctacaccgcc gagcgcttca tctggctgac caagctgaac aacctgcgca
     1561 tcctggagca gggcagcgag cgccccctga ccgacaccga gcgcgccacc ctgatggacg
     1621 agccctaccg caagagcaag ctgacctacg cccaggcccg caagctgctg ggtctggagg
     1681 acaccgcctt cttcaagggc ctgcgctacg gcaaggacaa cgccgaggcc agcaccctga
     1741 tggagatgaa ggcctaccac gccatcagcc gcgccctgga gaaggagggc ctgaaggaca
     1801 agaagagtcc tctgaacctg agccccgagc tgcaggacga gatcggcacc gccttcagcc
     1861 tgttcaagac cgacgaggac atcaccggcc gcctgaagga ccgcatccag cccgagatcc
     1921 tggaggccct gctgaagcac atcagcttcg acaagttcgt gcagatcagc ctgaaggccc
     1981 tgcgccgcat cgtgcccctg atggagcagg gcaagcgcta cgacgaggcc tgcgccgaga
     2041 tctacggcga ccactacggc aagaagaaca ccgaggagaa gatctacctg cctcctatcc
     2101 ccgccgacga gatccgcaac cccgtggtgc tgcgcgccct gagccaggcc cgcaaggtga
     2161 tcaacggcgt ggtgcgccgc tacggcagcc ccgcccgcat ccacatcgag accgcccgcg
     2221 aggtgggcaa gagcttcaag gaccgcaagg agatcgagaa gcgccaggag gagaaccgca
     2281 aggaccgcga gaaggccgcc gccaagttcc gcgagtactt ccccaacttc gtgggcgagc
     2341 ccaagagcaa ggacatcctg aagctgcgcc tgtacgagca gcagcacggc aagtgcctgt
     2401 acagcggcaa ggagatcaac ctgggccgcc tgaacgagaa gggctacgtg gagatcgccg
     2461 ctgccctgcc cttcagccgc acctgggacg acagcttcaa caacaaggtg ctggtgctgg
     2521 gcagcgaggc tcagaacaag ggcaaccaga ccccctacga gtacttcaac ggcaaggaca
     2581 acagccgcga gtggcaggag ttcaaggccc gcgtggagac cagccgcttc ccccgcagca
     2641 agaagcagcg catcctgctg cagaagttcg acgaggacgg cttcaaggag cgcaacctga
     2701 acgacacccg ctacgtgaac cgcttcctgt gccagttcgt ggccgaccgc atgcgcctga
     2761 ccggcaaggg caagaagcgc gtgttcgcca gcaacggcca gatcaccaac ctgctgcgcg
     2821 gcttctgggg cctgcgcaag gtgcgcgccg agaacgaccg ccaccacgcc ctggacgccg
     2881 tggtggtggc ctgcagcacc gtggccatgc agcagaagat cacccgcttc gtgcgctaca
     2941 aggagatgaa cgccttcgac ggtaaaacca tcgacaagga gaccggcgag gtgctgcacc
     3001 agaagaccca cttcccccag ccctgggagt tcttcgccca ggaggtgatg atccgcgtgt
     3061 tcggcaagcc cgacggcaag cccgagttcg aggaggccga cacccccgag aagctgcgca
     3121 ccctgctggc cgagaagctg agcagccgcc ctgaggccgt gcacgagtac gtgactcctc
     3181 tgttcgtgag ccgcgccccc aaccgcaaga tgagcggtca gggtcacatg gagaccgtga
     3241 agagcgccaa gcgcctggac gagggcgtga gcgtgctgcg cgtgcccctg acccagctga
     3301 agctgaagga cctggagaag atggtgaacc gcgagcgcga gcccaagctg tacgaggccc
     3361 tgaaggcccg cctggaggcc cacaaggacg accccgccaa ggccttcgcc gagcccttct
     3421 acaagtacga caaggccggc aaccgcaccc agcaggtgaa ggccgtgcgc gtggagcagg
     3481 tgcagaagac cggcgtgtgg gtgcgcaacc acaacggcat cgccgacaac gccaccatgg
     3541 tgcgcgtgga cgtgttcgag aagggcgaca agtactacct ggtgcccatc tacagctggc
     3601 aggtggccaa gggcatcctg cccgaccgcg ccgtggtgca gggcaaggac gaggaggact
     3661 ggcagctgat cgacgacagc ttcaacttca agttcagcct gcaccccaac gacctggtgg
     3721 aggtgatcac caagaaggcc cgcatgttcg gctacttcgc cagctgccac cgcggcaccg
     3781 gcaacatcaa catccgcatc cacgacctgg accacaagat cggcaagaac ggcatcctgg
     3841 agggcatcgg cgtgaagacc gccctgagct tccagaagta ccagatcgac gagctgggca
     3901 aggagatccg cccctgccgc ctgaagaagc gccctcctgt gcgcagcagg gctgacccca
     3961 agaagaagag gaaggtggag gccagcgggc gcgccatttt caaaccagaa gaactacgac
     4021 aggcactgat gccaactttg gaggcacttt accgtcagga tccagaatcc cttccctttc
     4081 gtcaacctgt ggaccctcag cttttaggaa tccctgatta ctttgatatt gtgaagagcc
     4141 ccatggatct ttctaccatt aagaggaagt tagacactgg acagtatcag gagccctggc
     4201 agtatgtcga tgatatttgg cttatgttca ataatgcctg gttatataac cggaaaacat
     4261 cacgggtata caaatactgc tccaagctct ctgaggtctt tgaacaagaa attgacccag
     4321 tgatgcaaag ccttggatac tgttgtggca gaaagttgga gttctctcca cagacactgt
     4381 gttgctacgg caaacagttg tgcacaatac ctcgtgatgc cacttattac agttaccaga
     4441 acaggtatca tttctgtgag aagtgtttca atgagatcca aggggagagc gtttctttgg
     4501 gggatgaccc ttcccagcct caaactacaa taaataaaga acaattttcc aagagaaaaa
     4561 atgacacact ggatcctgaa ctgtttgttg aatgtacaga gtgcggaaga aagatgcatc
     4621 agatctgtgt ccttcaccat gagatcatct ggcctgctgg attcgtctgt gatggctgtt
     4681 taaagaaaag tgcacgaact aggaaagaaa ataagttttc tgctaaaagg ttgccatcta
     4741 ccagacttgg cacctttcta gagaatcgtg tgaatgactt tctgaggcga cagaatcacc
     4801 ctgagtcagg agaggtcact gttagagtag ttcatgcttc tgacaaaacc gtggaagtaa
     4861 aaccaggcat gaaagcaagg tttgtggaca gtggagagat ggcagaatcc tttccatacc
     4921 gaaccaaagc cctctttgcc tttgaagaaa ttgatggtgt tgacctgtgc ttctttggca
     4981 tgcatgttca agagtatggc tctgactgcc ctccacccaa ccagaggaga gtatacatat
     5041 cttacctcga tagtgttcat ttcttccgtc ctaaatgctt gaggactgca gtctatcatg
     5101 aaatcctaat tggatattta gaatatgtca agaaattagg ttacacaaca gggcatattt
     5161 gggcatgtcc accaagtgag ggagatgatt atatcttcca ttgccatcct cctgaccaga
     5221 agatacccaa gcccaagcga ctgcaggaat ggtacaaaaa aatgcttgac aaggctgtat
     5281 cagagcgtat tgtccatgac tacaaggata tttttaaaca agctactgaa gatagattaa
     5341 caagtgcaaa ggaattgcct tatttcgagg gtgatttctg gcccaatgtt ctggaagaaa
     5401 gcattaagga actggaacag gaggaagaag agagaaaacg agaggaaaac accagcaatg
     5461 aaagcacaga tgtgaccaag ggagacagca aaaatgctaa aaagaagaat aataagaaaa
     5521 ccagcaaaaa taagagcagc ctgagtaggg gcaacaagaa gaaacccggg atgcccaatg
     5581 tatctaacga cctctcacag aaactatatg ccaccatgga gaagcataaa gaggtcttct
     5641 ttgtgatccg cctcattgct ggccctgctg ccaactccct gcctcccatt gttgatcctg
     5701 atcctctcat cccctgcgat ctgatggatg gtcgggatgc gtttctcacg ctggcaaggg
     5761 acaagcacct ggagttctct tcactccgaa gagcccagtg gtccaccatg tgcatgctgg
     5821 tggagctgca cacgcagagc caggactacc cgtacgacgt tccggactac gcttcttgaa
     5881 ccggttagta atgagtttaa acgggggagg ctaactgaaa cacggaagga gacaataccg
     5941 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc
     6001 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     6061 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     6121 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcagatct
     6181 gcgcagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     6241 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6301 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6361 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6421 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6481 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6541 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6601 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     6661 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6721 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6781 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6841 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6901 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     6961 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     7021 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     7081 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     7141 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     7201 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     7261 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     7321 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     7381 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     7441 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     7501 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     7561 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     7621 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     7681 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     7741 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     7801 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     7861 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     7921 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     7981 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     8041 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8101 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     8161 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8221 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8281 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8341 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8401 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8461 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8521 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8581 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8641 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8701 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8761 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8821 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8881 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8941 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9001 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9061 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9121 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9181 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9241 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9301 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9361 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9421 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9481 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9541 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9601 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9661 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9721 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9781 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9841 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9901 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9961 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10021 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10081 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10141 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10201 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10261 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10321 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
    10381 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
    10441 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
    10501 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
    10561 cgggccagat atacgcgtt
//
