LOCUS       sequence-244298-       12604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FLT4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12604)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-FLT4
FEATURES             Location/Qualifiers
     source          1..12604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7541..7565)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7618..7659
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7680..8247
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7697..8249
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7841..7858)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8068..8087
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8261..8263,8264..8266,8267..8980)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8261..8263,8264..8266,8267..8977)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8306..8327)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8567..8586)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8914..8935
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8994..9582
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9047..9067)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9465..9476)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9657..9890
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10009..10027)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10095..10199
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10200..10268,10269..11060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10418..10437)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11231..11819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11720..11739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11973..11990
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12230..12251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12266..12271,12272..12289,12290..12296)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12304..12320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12309..12331
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12328..12344
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12328..12344
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12394..12413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg cagcggggcg ccgcgctgtg cctgcgactg
     3481 tggctctgcc tgggactcct ggacggcctg gtgagtggct actccatgac ccccccgacc
     3541 ttgaacatca cggaggagtc acacgtcatc gacaccggtg acagcctgtc catctcctgc
     3601 aggggacagc accccctcga gtgggcttgg ccaggagctc aggaggcgcc agccaccgga
     3661 gacaaggaca gcgaggacac gggggtggtg cgagactgcg agggcacaga cgccaggccc
     3721 tactgcaagg tgttgctgct gcacgaggta catgccaacg acacaggcag ctacgtctgc
     3781 tactacaagt acatcaaggc acgcatcgag ggcaccacgg ccgccagctc ctacgtgttc
     3841 gtgagagact ttgagcagcc attcatcaac aagcctgaca cgctcttggt caacaggaag
     3901 gacgccatgt gggtgccctg tctggtgtcc atccccggcc tcaatgtcac gctgcgctcg
     3961 caaagctcgg tgctgtggcc agacgggcag gaggtggtgt gggatgaccg gcggggcatg
     4021 ctcgtgtcca cgccactgct gcacgatgcc ctgtacctgc agtgcgagac cacctgggga
     4081 gaccaggact tcctttccaa ccccttcctg gtgcacatca caggcaacga gctctatgac
     4141 atccagctgt tgcccaggaa gtcgctggag ctgctggtag gggagaagct ggtcctgaac
     4201 tgcaccgtgt gggctgagtt taactcaggt gtcacctttg actgggacta cccagggaag
     4261 caggcagagc ggggtaagtg ggtgcccgag cgacgctccc agcagaccca cacagaactc
     4321 tccagcatcc tgaccatcca caacgtcagc cagcacgacc tgggctcgta tgtgtgcaag
     4381 gccaacaacg gcatccagcg atttcgggag agcaccgagg tcattgtgca tgaaaatccc
     4441 ttcatcagcg tcgagtggct caaaggaccc atcctggagg ccacggcagg agacgagctg
     4501 gtgaagctgc ccgtgaagct ggcagcgtac cccccgcccg agttccagtg gtacaaggat
     4561 ggaaaggcac tgtccgggcg ccacagtcca catgccctgg tgctcaagga ggtgacagag
     4621 gccagcacag gcacctacac cctcgccctg tggaactccg ctgctggcct gaggcgcaac
     4681 atcagcctgg agctggtggt gaatgtgccc ccccagatac atgagaagga ggcctcctcc
     4741 cccagcatct actcgcgtca cagccgccag gccctcacct gcacggccta cggggtgccc
     4801 ctgcctctca gcatccagtg gcactggcgg ccctggacac cctgcaagat gtttgcccag
     4861 cgtagtctcc ggcggcggca gcagcaagac ctcatgccac agtgccgtga ctggagggcg
     4921 gtgaccacgc aggatgccgt gaaccccatc gagagcctgg acacctggac cgagtttgtg
     4981 gagggaaaga ataagactgt gagcaagctg gtgatccaga atgccaacgt gtctgccatg
     5041 tacaagtgtg tggtctccaa caaggtgggc caggatgagc ggctcatcta cttctatgtg
     5101 accaccatcc ccgacggctt caccatcgaa tccaagccat ccgaggagct actagagggc
     5161 cagccggtgc tcctgagctg ccaagccgac agctacaagt acgagcatct gcgctggtac
     5221 cgcctcaacc tgtccacgct gcacgatgcg cacgggaacc cgcttctgct cgactgcaag
     5281 aacgtgcatc tgttcgccac ccctctggcc gccagcctgg aggaggtggc acctggggcg
     5341 cgccacgcca cgctcagcct gagtatcccc cgcgtcgcgc ccgagcacga gggccactat
     5401 gtgtgcgaag tgcaagaccg gcgcagccat gacaagcact gccacaagaa gtacctgtcg
     5461 gtgcaggccc tggaagcccc tcggctcacg cagaacttga ccgacctcct ggtgaacgtg
     5521 agcgactcgc tggagatgca gtgcttggtg gccggagcgc acgcgcccag catcgtgtgg
     5581 tacaaagacg agaggctgct ggaggaaaag tctggagtcg acttggcgga ctccaaccag
     5641 aagctgagca tccagcgcgt gcgcgaggag gatgcgggac gctatctgtg cagcgtgtgc
     5701 aacgccaagg gctgcgtcaa ctcctccgcc agcgtggccg tggaaggctc cgaggataag
     5761 ggcagcatgg agatcgtgat ccttgtcggt accggcgtca tcgctgtctt cttctgggtc
     5821 ctcctcctcc tcatcttctg taacatgagg aggccggccc acgcagacat caagacgggc
     5881 tacctgtcca tcatcatgga ccccggggag gtgcctctgg aggagcaatg cgaatacctg
     5941 tcctacgatg ccagccagtg ggaattcccc cgagagcggc tgcacctggg gagagtgctc
     6001 ggctacggcg ccttcgggaa ggtggtggaa gcctccgctt tcggcatcca caagggcagc
     6061 agctgtgaca ccgtggccgt gaaaatgctg aaagagggcg ccacggccag cgagcaccgc
     6121 gcgctgatgt cggagctcaa gatcctcatt cacatcggca accacctcaa cgtggtcaac
     6181 ctcctcgggg cgtgcaccaa gccgcagggc cccctcatgg tgatcgtgga gttctgcaag
     6241 tacggcaacc tctccaactt cctgcgcgcc aagcgggacg ccttcagccc ctgcgcggag
     6301 aagtctcccg agcagcgcgg acgcttccgc gccatggtgg agctcgccag gctggatcgg
     6361 aggcggccgg ggagcagcga cagggtcctc ttcgcgcggt tctcgaagac cgagggcgga
     6421 gcgaggcggg cttctccaga ccaagaagct gaggacctgt ggctgagccc gctgaccatg
     6481 gaagatcttg tctgctacag cttccaggtg gccagaggga tggagttcct ggcttcccga
     6541 aagtgcatcc acagagacct ggctgctcgg aacattctgc tgtcggaaag cgacgtggtg
     6601 aagatctgtg actttggcct tgcccgggac atctacaaag accccgacta cgtccgcaag
     6661 ggcagtgccc ggctgcccct gaagtggatg gcccctgaaa gcatcttcga caaggtgtac
     6721 accacgcaga gtgacgtgtg gtcctttggg gtgcttctct gggagatctt ctctctgggg
     6781 gcctccccgt accctggggt gcagatcaat gaggagttct gccagcggct gagagacggc
     6841 acaaggatga gggccccgga gctggccact cccgccatac gccgcatcat gctgaactgc
     6901 tggtccggag accccaaggc gagacctgca ttctcggagc tggtggagat cctgggggac
     6961 ctgctccagg gcaggggcct gcaagaggaa gaggaggtct gcatggcccc gcgcagctct
     7021 cagagctcag aagagggcag cttctcgcag gtgtccacca tggccctaca catcgcccag
     7081 gctgacgctg aggacagccc gccaagcctg cagcgccaca gcctggccgc caggtattac
     7141 aactgggtgt cctttcccgg gtgcctggcc agaggggctg agacccgtgg ttcctccagg
     7201 atgaagacat ttgaggaatt ccccatgacc ccaacgacct acaaaggctc tgtggacaac
     7261 cagacagaca gtgggatggt gctggcctcg gaggagtttg agcagataga gagcaggcat
     7321 agacaagaaa gcggcttcag ctgtaaagga cctggccaga atgtggctgt gaccagggca
     7381 caccctgact cccaagggag gcggcggcgg cctgagcggg gggcccgagg aggccaggtg
     7441 ttttacaaca gcgagtatgg ggagctgtcg gagccaagcg aggaggacca ctgctccccg
     7501 tctgcccgcg tgactttctt cacagacaac agctactaga acccagcttt cttgtacaaa
     7561 gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt
     7621 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagat
     7681 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     7741 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     7801 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     7861 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     7921 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     7981 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     8041 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     8101 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     8161 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     8221 gttttccttt gaaaaacacg atgataatat ggccacacat atggtgagca agggcgagga
     8281 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     8341 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     8401 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     8461 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     8521 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     8581 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     8641 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     8701 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     8761 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     8821 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     8881 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     8941 cgccgggatc actctcggca tggacgagct gtacaagtaa atcgatagat cctaatcaac
     9001 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     9061 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     9121 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     9181 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     9241 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     9301 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     9361 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     9421 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     9481 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     9541 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc
     9601 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     9661 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     9721 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9781 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9841 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     9901 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     9961 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac
    10021 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
    10081 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    10141 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    10201 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    10261 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    10321 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    10381 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    10441 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10501 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10561 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    10621 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    10681 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    10741 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    10801 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    10861 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    10921 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    10981 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    11041 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    11101 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    11161 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    11221 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    11281 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    11341 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    11401 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    11461 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    11521 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    11581 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11641 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11701 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11761 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    11821 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    11881 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    11941 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    12001 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
    12061 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    12121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
    12181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    12241 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    12301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
    12361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    12421 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
    12481 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
    12541 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    12601 tagt
//
