LOCUS       sequence-244302-        1574 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calmodulin 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Chang JY, Nakahata Y, Hayano Y, Yasuda R
  TITLE     Mechanisms of Ca(2+)/calmodulin-dependent kinase II activation in 
            single dendritic spines.
  JOURNAL   Nat Commun. 2019 Jun 25;10(1):2784. doi: 10.1038/s41467-019-10694-z.
  PUBMED    31239443
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-06-25"; volume: "10(1):2784. doi"; pages: " 
            10.1038/s41467-019-10694-z"
COMMENT     Sequence Label: pCAG-mCherry-CaM
FEATURES             Location/Qualifiers
     source          1..1574
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      57..66
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             63..770
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(208..226)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     476..495
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     670..693
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             795..1244
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPT
                     EAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAA
                     ELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     complement(1271..1290)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1336..1391
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
ORIGIN
        1 ctgggcacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gctagcgcca
       61 ccatggtgag caagggcgag gaggacaaca tggccatcat caaggagttc atgcgcttca
      121 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      181 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
      241 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
      301 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
      361 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
      421 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
      481 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
      541 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
      601 ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
      661 tcaacatcaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
      721 agcgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag tccggactca
      781 gatctcgagc tcaaatggct gatcagctga ctgaagagca gattgctgaa ttcaaggaag
      841 ctttctccct attcgataaa gatggtgacg gcaccatcac aaccaaggaa ctggggaccg
      901 tcatgcggtc actgggtcag aacccaacag aagccgagct gcaggatatg atcaacgaag
      961 tggatgctga tggcaatggc accattgact tcccagagtt cttgactatg atggctagaa
     1021 aaatgaaaga cacagatagc gaagaagaga tccgcgaggc cttccgagtg tttgacaagg
     1081 atgggaatgg ttacatcagt gcggcagaac tgcgccacgt catgacaaac ttaggagaaa
     1141 agctaacaga tgaagaagta gatgaaatga tcagagaagc agatattgat ggcgacggac
     1201 aagtcaacta tgaagaattc gtacagatga tgactgcaaa atgagcggcc gcgactctag
     1261 agtcgacgga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     1321 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     1381 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     1441 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     1501 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     1561 aagccttgac ttga
//
