LOCUS       sequence-244336-       12996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for C-terminus tagging of 
            human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12996)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-63: ACTN2-mEGFP
FEATURES             Location/Qualifiers
     source          1..12996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        113..492
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    508..629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(519..538)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     573..592
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1732..1749)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1732..1748)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1741..1763)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1957..1976)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2076..2098
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2136..2154)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2222..2326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2327..2395,2396..3187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2545..2564)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3358..3946
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3847..3866
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4100..4117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4234..4255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4270..4275,4276..4293,4294..4300)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4308..4324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4313..4335
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4332..4348
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4332..4348
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4421..4632
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4645..4913
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             join(4947..4949,4950..4952,4953..5663)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             5685..5741
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6570..6626
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(7850..7869)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7915..7970
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7969..7988)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     CDS             complement(join(9308..10021,10022..10024))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    10105..10329
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(10346..10945)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10429..10449)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     10926..10945
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(10946..10999)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(11290..11309)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     11559..11577
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(11784..11803)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          11811..12828
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(11865..11887)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(join(12830..12996,1..111))
                     /label=chicken beta-actin promoter
ORIGIN
        1 cccccgcccc catcgctgca caaaataatt aaaaaataaa taaatacaaa attgggggtg
       61 gggagggggg ggagatgggg agagtgaagc agaacgtggg gctcacctcg accatggtaa
      121 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
      181 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
      241 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
      301 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
      361 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
      421 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca
      481 ataatcaatg tcgagactca gacatgataa gatacattga tgagtttgga caaaccacaa
      541 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
      601 taaccattat aagctgcaat aaacaagtta tcggaatact aatataactt cgtatatcct
      661 acactatacg aagttattgg tatgtcggga acctctccgg gcccagcagt cccgtcgacc
      721 agatcgctct cgccgtacag tgcggaagag aacgcagcgt aatccagtgc accaggcaca
      781 ctgcctgggc ccgagtaggc gggcatcctc ttgatgcagt actgggcctg atccgggggc
      841 agctcccgac gcagctcctc cgccaggatg tatggctggg aaaaaataca cacgtgtttg
      901 cagtcagttg cagaaacacc acaacataaa cagtcttaat acttacatat ttcttgcatc
      961 tgtttaattt tttaaaagga ctaaagaata gggagtctat tggcatagta gtaccagaca
     1021 accaacagaa agccaagttt tacttgtagg agcgtatcta gaagcggcct ggccagcagg
     1081 aagggtgata tgcttcataa aggcaaatgg agcattacta gtagttctca aactgagcca
     1141 caatgcgagg atacgtacta tgatctgcat ttcataccag aaaagatgaa gcttttaaaa
     1201 aaaaagttca gtgggaaata ggacaattcc agccaaatag tctggttggt aaaagtcaaa
     1261 ataagtaaat ctcaaatatc acattataat ttatatcatt atagtttgca tattatataa
     1321 tttatagtag aagtttctta gtggggttgg aggagccatg gtttcttacc ttctaaatca
     1381 gctgggctat ttcccatcca ccctcacccc atgggtaaat gattcatttt aaagaaaaca
     1441 ttattttaaa aatagttggt cttatctgat ttttaaggta ataaatatgg ctctgttact
     1501 gtgttttagc atgctaacct ccttgtggta atgttcatta tcctctcagc cagaaaacag
     1561 ggtaaaataa tcctgtccca aattgaattt atacatagag atgtttttat tgtttggggg
     1621 gtaaaatatt acaattctga actggcaatt aaactcctcc tgaaggtcta ggctacatgt
     1681 aatgaaaagc ttgccaattt tttactgact gaacggctaa cgcgtcaatt cactggccgt
     1741 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     1801 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     1861 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     1921 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     1981 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     2041 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     2101 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     2161 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     2221 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2281 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     2341 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     2401 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     2461 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     2521 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     2581 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     2641 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     2701 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     2761 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     2821 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     2881 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     2941 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3001 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3061 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3121 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3181 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     3241 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     3301 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3361 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3421 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3481 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3541 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3601 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3661 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3721 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3781 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3841 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3901 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3961 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4021 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4081 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     4141 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     4201 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     4261 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     4321 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctcggcg cgccattggg
     4381 atgttaacaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     4441 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     4501 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     4561 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     4621 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     4681 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     4741 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     4801 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     4861 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     4921 tgaccggcgc ctacctcgag cccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     4981 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     5041 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     5101 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gtcctggggc gtgcagtgct
     5161 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     5221 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     5281 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     5341 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc gacaacgtct
     5401 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     5461 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     5521 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
     5581 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     5641 tcggcatgga cgagctgtac aagggtgcta gcaccggaag cggagctact aacttcagcc
     5701 tgctgaagca ggctggagac gtggaggaga accctggacc tactatgaaa aagcctgaac
     5761 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     5821 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     5881 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     5941 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga
     6001 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
     6061 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg
     6121 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     6181 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     6241 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     6301 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
     6361 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
     6421 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
     6481 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cggggcgtat
     6541 atgctccgca ttggtctagt ggctcaggcg caacaaactt ctctctcctc aaacaagccg
     6601 gcgatgtcga agagaaccca ggcccaatgg cttcttaccc tggacaccag catgcttctg
     6661 cctttgacca ggctgccaga tccaggggcc actccaacag gagaactgcc ctaagaccca
     6721 gaagacagca ggaagccact gaggtgaggc ctgagcagaa gatgccaacc ctgctgaggg
     6781 tgtacattga tggacctcat ggcatgggca agaccaccac cactcaactg ctggtggcac
     6841 tgggctccag ggatgacatt gtgtatgtgc ctgagccaat gacctactgg agagtgctag
     6901 gagcctctga gaccattgcc aacatctaca ccacccagca caggctggac cagggagaaa
     6961 tctctgctgg agatgctgct gtggtgatga cctctgccca gatcacaatg ggaatgccct
     7021 atgctgtgac tgatgctgtt ctggctcctc acattggagg agaggctggc tcttctcatg
     7081 cccctccacc tgccctgacc ctgatctttg acagacaccc cattgcagcc ctgctgtgct
     7141 acccagcagc aaggtacctc atgggctcca tgaccccaca ggctgtgctg gcttttgtgg
     7201 ccctgatccc tccaaccctc cctggcacca acattgttct gggagcactg cctgaagaca
     7261 gacacattga caggctggca aagaggcaga gacctggaga gagactggac ctggccatgc
     7321 tggctgcaat cagaagggtg tatggactgc tggcaaacac tgtgagatac ctccagtgtg
     7381 gaggctcttg gagagaggac tggggacagc tctctggaac agcagtgccc cctcaaggag
     7441 ctgagcccca gtccaatgct ggtccaagac cccacattgg ggacaccctg ttcaccctgt
     7501 tcagagcccc tgagctgctg gctcccaatg gagacctgta caatgtgttt gcctgggctc
     7561 tggatgttct agccaagagg ctgaggtcca tgcatgtgtt catcctggac tatgaccagt
     7621 cccctgctgg atgcagagat gctctgctgc aactaacctc tggcatggtg cagacccatg
     7681 tgaccacccc tggcagcatc cccaccatct gtgacctagc cagaaccttt gccagggaga
     7741 tgggagaggc caactaagct agctagaccg gtactcctca ggtgcaggct gcctatcaga
     7801 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
     7861 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     7921 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     7981 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     8041 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     8101 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     8161 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     8221 ctgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     8281 ttctcttatg gagatcgtta acatttaaat ctctcctact ctaactctgg cttctcctta
     8341 accggtcata aaactagagg cataaggaag tcctgactgg agagctagaa ggatactggt
     8401 ttctgacttg ccttgtgatg aaccagtcag tactacagaa acctgcttat ttataaacag
     8461 agcactttta tgtggtcatc tcttgagaac ctgtactaat aagctctaac aaaaagcatg
     8521 ggaacctgga atcaataaat aatcctaaaa acctcatttt acctagtgat tgcatccttc
     8581 tctctggcaa ttaaactgac ttccacgtgt ccttaggtct ctattctgga ggataagaga
     8641 agcatcaaaa aatcactgac tgagttgacc atccagccct aaataatgaa gagatgacat
     8701 aaactatttt aaaaattgta agtgccaaaa atcttttggg tagtcttttt aatccccggc
     8761 agttccatct cacaatatta aattcatttg aagatacaat agtaatcttt ggataagcat
     8821 gaaaataaaa cattagaatc ccaaatatac tcccctaggg gcaatcacat aagcatatta
     8881 gagataaatg aaatttttta caaatagcaa acctttattt accaatttgt ggttttttcc
     8941 ccccggtctt aaatcttggg tattttgtat tttcacattt ttcactttcc ttctgtccct
     9001 agatcctcct aatttcctat tactcaagta atttgttctg tttaatcaaa gaaatcaacc
     9061 tgatatttca ttacattttg ctaaaaactc agaaataaac ctatttatga agcgcactat
     9121 gtcatatact gtaaaaactt cattttagcc gtgttatatt gctggcaatt actgaactac
     9181 gaaaggcttt tttttttccc ctctctctct ctcaacttaa taaagcttgt caaagtttcc
     9241 aggaaacaaa ctgtgacttc cgcttttatt gcattctgat gccatgagtg attacagaag
     9301 ctcagcatca cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     9361 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgggtgc
     9421 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     9481 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     9541 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     9601 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     9661 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     9721 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     9781 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     9841 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     9901 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     9961 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
    10021 tcacagtagc aatggatccg ggcccggaga ggttcccgac atacctatat aacttcgtat
    10081 atcctacact atacgaagtt atttccatag agcccaccgc atccccagca tgcctgctat
    10141 tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac
    10201 acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg
    10261 caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag
    10321 aaggcacagg agtacctcac ttaagtcagg caccgggctt gcgggtcatg caccaggtgc
    10381 gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg
    10441 ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct
    10501 ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagactc
    10561 cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca
    10621 tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg
    10681 cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt
    10741 cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca
    10801 gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt
    10861 cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc
    10921 gcaccgtggg cttgtactcg gtcattgggc caggattctc ctcgacgtca ccgcatgtga
    10981 gcagacttcc tctgccctcc ttaagtgcgg ccgctttata gagttcatcc attccgccgg
    11041 tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg
    11101 aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg
    11161 ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct
    11221 gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc
    11281 agcccatggt cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct
    11341 tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc
    11401 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca
    11461 agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag
    11521 gggacaggat gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttggcgg
    11581 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt
    11641 tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat
    11701 cttcttctcc tttggagacc atggtaagct cttcgaacgg tggatccccg gtggcggaat
    11761 tctttgccaa aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa
    11821 aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag
    11881 ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg acagccccga
    11941 ggctggagag ggagaagggg acggcggcgc ggcgacgcac gaaggccctc cccgcccatt
    12001 tccttcctgc cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt gcggcggcgc
    12061 ctcccagatt tcggctccgc acagatttgg gacaaaggaa gtccctgcgc cctctcgcac
    12121 gattaccata aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc ggcgctccgg
    12181 gggccgccgc gccccccccc cgagcccccc ccggcccgag gcggccccgc cccgcccggc
    12241 acccccacct gccgccaccc cccgcccggc acggcgagcc ccgcgccacg ccccgtacgg
    12301 agccccgcac ccgaagccgg gccgtgctca gcaactcggg gaggggggtg cagggggggt
    12361 tgcagcccga ccgacgcgcc cacaccccct gctcaccccc ccacgcacac accccgcacg
    12421 cagcctttgt tcccctcgca gccccccccg caccgcgggg caccgccccc ggccgcgctc
    12481 ccctcgcgca cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg ctcacagccg
    12541 ccgggcagcg cggagccgca cgcggcgctc cccacgcaca cacacacgca cgcacccccc
    12601 gagccgctcc ccccgcacaa agggccctcc cggagcccct caaggctttc acgcagccac
    12661 agaaaagaaa caagccgtca ttaaaccaag cgctaattac agcccggagg agaagggccg
    12721 tcccgcccgc tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg gcggcgcgag
    12781 gcggcggcgg agcggggcac ggggcgaagg cagcgtcgca gcgactcccc gcccgccgcg
    12841 cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga aactttcgga
    12901 gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc cgcccctcgc
    12961 cccgccccgc cccgcctggc gcgcgccccc cccccc
//
