LOCUS       sequence-244354-        3008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Five prime entry vector containing 349bp cldn15la promoter from 
            zebrafish. Drives expression in intestinal epithelial cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Murdoch CC, Espenschied ST, Matty MA, Mueller O, Tobin DM, Rawls JF
  TITLE     Intestinal Serum amyloid A suppresses systemic neutrophil activation
            and bactericidal activity in response to microbiota colonization.
  JOURNAL   PLoS Pathog. 2019 Mar 7;15(3):e1007381. doi: 
            10.1371/journal.ppat.1007381. eCollection 2019 Mar.
  PUBMED    30845179
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2019-03-7"; volume: "15(3):e1007381. doi"; pages: "
            10.1371/journal.ppat.1007381. eCollection 2019 Mar"
COMMENT     Sequence Label: p5E-0.349cldn15la
FEATURES             Location/Qualifiers
     source          1..3008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1054..1177
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1249..1268)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1250..1268)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1273..1289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1273..1289)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1391..1410)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1402..2211
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1469..1488)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2358..2946
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2847..2866
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggg ccggccgctg ttcacagtga ttccctcact
      721 aatggtagtt tatctggcga ctaagattac ttattttcaa tgcagccata tatcagtttc
      781 aatacaatca gagtgaaact ccaaccatat atgataacca aacatggtaa tggtgttatc
      841 agggtaaatg tgtgtgtctc gctagacaaa atgctcacac ccatcatcaa ctcataagct
      901 ggacagggtg cggcgatggc tttatgaccc tccagaaggt tgaagtcctg cagctcaaca
      961 gaagagcaat ccaaacccat cgatcaggag caagaacagg acacctatac agattcttcc
     1021 cttgaatttt ggatttctga ggttgggcgc gcccaagttt gtacaaaaaa gttgaacgag
     1081 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
     1141 cataatactg taaaacacaa catatgcagt cactatgaac caactactta gatggtatta
     1201 gtgacctgta gaattcgagc tctagagctg cagggcggcc gcgatatccc ctatagtgag
     1261 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     1321 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1381 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     1441 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     1501 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     1561 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     1621 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     1681 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     1741 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     1801 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     1861 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     1921 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     1981 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2041 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2101 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2161 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     2221 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     2281 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     2341 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2401 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2461 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2521 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2581 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2641 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2701 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2761 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2821 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2881 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2941 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3001 cacatgtt
//
